Longevity of Apis mellifera workers fed on a diet incorporating entomopathogens
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Arquivos do instituto biológico (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1808-16572019000100224 |
Resumo: | ABSTRACT: The present study had the objective of evaluating the longevity of A. mellifera workers fed on a diet incorporating commercial entomopathogens, Beauveria bassiana, and Bacillus thuringiensis. It also aimed at verifying possible morphological alterations in the midgut. To this purpose, the entomopathogens used were B. bassiana (Product A) (5.0 × 1011 viable conidia.kg-1), B. thuringiensis (Product B) (2.5 × 109 viable spores.g-1), and B. thuringiensis (Product C) (1.0 × 109 viable spores.g-1); and two controls: T1: sterilized distilled water, and T2: sterilized distilled water + Tween 80® (0.01%). For the bioassays, 2 mL of each treatment were incorporated into Candy paste. For each treatment, 80 bees were individually in flat bottom glass tubes (2.5 cm Ø) covered with voile, containing a piece of cotton soaked in water and Candy paste. These tubes were stored in a B.O.D (30 ± 2°C, R.H 70% ± 10%, 12 h), and mortality was evaluated every six hours, for 10 days. Soon after verifying mortality, two bees per treatment were selected for the removal of their midgut. Midgut samples were processed using standard methodology for Scanning Electron Microscopy (SEM). It was verified that products A, B, and C reduced the longevity of bees when compared to T1 and T2 controls. In the qualitative analyses carried out using SEM, it was not possible to observe external or internal morphological alterations to midgut tissues. Although products A, B, and C cause a reduction in longevity, their presence was not verified when tissues were analyzed using SEM. |
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Longevity of Apis mellifera workers fed on a diet incorporating entomopathogensAfricanized beebiological insecticidesselectivityABSTRACT: The present study had the objective of evaluating the longevity of A. mellifera workers fed on a diet incorporating commercial entomopathogens, Beauveria bassiana, and Bacillus thuringiensis. It also aimed at verifying possible morphological alterations in the midgut. To this purpose, the entomopathogens used were B. bassiana (Product A) (5.0 × 1011 viable conidia.kg-1), B. thuringiensis (Product B) (2.5 × 109 viable spores.g-1), and B. thuringiensis (Product C) (1.0 × 109 viable spores.g-1); and two controls: T1: sterilized distilled water, and T2: sterilized distilled water + Tween 80® (0.01%). For the bioassays, 2 mL of each treatment were incorporated into Candy paste. For each treatment, 80 bees were individually in flat bottom glass tubes (2.5 cm Ø) covered with voile, containing a piece of cotton soaked in water and Candy paste. These tubes were stored in a B.O.D (30 ± 2°C, R.H 70% ± 10%, 12 h), and mortality was evaluated every six hours, for 10 days. Soon after verifying mortality, two bees per treatment were selected for the removal of their midgut. Midgut samples were processed using standard methodology for Scanning Electron Microscopy (SEM). It was verified that products A, B, and C reduced the longevity of bees when compared to T1 and T2 controls. In the qualitative analyses carried out using SEM, it was not possible to observe external or internal morphological alterations to midgut tissues. Although products A, B, and C cause a reduction in longevity, their presence was not verified when tissues were analyzed using SEM.Instituto Biológico2019-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1808-16572019000100224Arquivos do Instituto Biológico v.86 2019reponame:Arquivos do instituto biológico (Online)instname:Instituto Biológico (IB)instacron:IBIO10.1590/1808-1657000492017info:eu-repo/semantics/openAccessColombo,Fernanda CarolineMaciel,Rodrigo Mendes AntunesLibardoni,GabrielaRaulino,FernandaLozano,Everton RicardiMaia,Fabiana Martins CostaFreitas,Patrícia FranchiNeves,Pedro Manuel Oliveira JaneiroPotrich,Micheleeng2019-10-16T00:00:00Zoai:scielo:S1808-16572019000100224Revistahttp://www.biologico.sp.gov.br/arquivos_bio.phphttps://old.scielo.br/oai/scielo-oai.php||arquivos@biologico.sp.gov.br1808-16570020-3653opendoar:2019-10-16T00:00Arquivos do instituto biológico (Online) - Instituto Biológico (IB)false |
dc.title.none.fl_str_mv |
Longevity of Apis mellifera workers fed on a diet incorporating entomopathogens |
title |
Longevity of Apis mellifera workers fed on a diet incorporating entomopathogens |
spellingShingle |
Longevity of Apis mellifera workers fed on a diet incorporating entomopathogens Colombo,Fernanda Caroline Africanized bee biological insecticides selectivity |
title_short |
Longevity of Apis mellifera workers fed on a diet incorporating entomopathogens |
title_full |
Longevity of Apis mellifera workers fed on a diet incorporating entomopathogens |
title_fullStr |
Longevity of Apis mellifera workers fed on a diet incorporating entomopathogens |
title_full_unstemmed |
Longevity of Apis mellifera workers fed on a diet incorporating entomopathogens |
title_sort |
Longevity of Apis mellifera workers fed on a diet incorporating entomopathogens |
author |
Colombo,Fernanda Caroline |
author_facet |
Colombo,Fernanda Caroline Maciel,Rodrigo Mendes Antunes Libardoni,Gabriela Raulino,Fernanda Lozano,Everton Ricardi Maia,Fabiana Martins Costa Freitas,Patrícia Franchi Neves,Pedro Manuel Oliveira Janeiro Potrich,Michele |
author_role |
author |
author2 |
Maciel,Rodrigo Mendes Antunes Libardoni,Gabriela Raulino,Fernanda Lozano,Everton Ricardi Maia,Fabiana Martins Costa Freitas,Patrícia Franchi Neves,Pedro Manuel Oliveira Janeiro Potrich,Michele |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Colombo,Fernanda Caroline Maciel,Rodrigo Mendes Antunes Libardoni,Gabriela Raulino,Fernanda Lozano,Everton Ricardi Maia,Fabiana Martins Costa Freitas,Patrícia Franchi Neves,Pedro Manuel Oliveira Janeiro Potrich,Michele |
dc.subject.por.fl_str_mv |
Africanized bee biological insecticides selectivity |
topic |
Africanized bee biological insecticides selectivity |
description |
ABSTRACT: The present study had the objective of evaluating the longevity of A. mellifera workers fed on a diet incorporating commercial entomopathogens, Beauveria bassiana, and Bacillus thuringiensis. It also aimed at verifying possible morphological alterations in the midgut. To this purpose, the entomopathogens used were B. bassiana (Product A) (5.0 × 1011 viable conidia.kg-1), B. thuringiensis (Product B) (2.5 × 109 viable spores.g-1), and B. thuringiensis (Product C) (1.0 × 109 viable spores.g-1); and two controls: T1: sterilized distilled water, and T2: sterilized distilled water + Tween 80® (0.01%). For the bioassays, 2 mL of each treatment were incorporated into Candy paste. For each treatment, 80 bees were individually in flat bottom glass tubes (2.5 cm Ø) covered with voile, containing a piece of cotton soaked in water and Candy paste. These tubes were stored in a B.O.D (30 ± 2°C, R.H 70% ± 10%, 12 h), and mortality was evaluated every six hours, for 10 days. Soon after verifying mortality, two bees per treatment were selected for the removal of their midgut. Midgut samples were processed using standard methodology for Scanning Electron Microscopy (SEM). It was verified that products A, B, and C reduced the longevity of bees when compared to T1 and T2 controls. In the qualitative analyses carried out using SEM, it was not possible to observe external or internal morphological alterations to midgut tissues. Although products A, B, and C cause a reduction in longevity, their presence was not verified when tissues were analyzed using SEM. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1808-16572019000100224 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1808-16572019000100224 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1808-1657000492017 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Biológico |
publisher.none.fl_str_mv |
Instituto Biológico |
dc.source.none.fl_str_mv |
Arquivos do Instituto Biológico v.86 2019 reponame:Arquivos do instituto biológico (Online) instname:Instituto Biológico (IB) instacron:IBIO |
instname_str |
Instituto Biológico (IB) |
instacron_str |
IBIO |
institution |
IBIO |
reponame_str |
Arquivos do instituto biológico (Online) |
collection |
Arquivos do instituto biológico (Online) |
repository.name.fl_str_mv |
Arquivos do instituto biológico (Online) - Instituto Biológico (IB) |
repository.mail.fl_str_mv |
||arquivos@biologico.sp.gov.br |
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1754193670384386048 |