Toxicity intraperitoneal and intragastric route of Bacillus thuringiensis and Melia azedarach in mice
Autor(a) principal: | |
---|---|
Data de Publicação: | 2012 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Arquivos do instituto biológico (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1808-16572012000400007 |
Resumo: | The aim of this investigation was the assessment of toxicity of two new isolates of Bacillus thuringiensis, and the aqueous extract of Melia azedarach through in vivo assays in CF1 mice. Bt 1958-2, Bt 2014-2 and the BTh Thuricide 63 standard isolates were grown in liquid usual glicosed medium, and Cry proteins were purified by centrifugation on a sucrose gradient. The supernatant was autoclaved at 121º C, 15min. to maintain the exotoxins. Dehydrated leaves of M. azedarach were used to prepare a 10% aqueous extract. Mice were treated either orally or intraperitoneally with a whole bacterial suspension (1.10(10) UFC/mL), a culture supernatant or purified crystal protein (50 µg/mL), and with the plant extract (50 µg/mL). The stomachs of the mice were collected and observed in stereomicroscopy, and the stomach contents were analyzed in 10% SDS-PAGE. Results showed that none of the oral treatments were toxic to mice, but intraperitoneal bacterial suspensions were lethal to the animals 6 - 24 hours after injection. In conclusion, the Cry proteins of the new B. thuringiensis isolates must be evaluated for their use as tools in the biotechnology field, since they do not show toxicity against mammals, intragastrically or peritoneally, just like the M. azedarach aqueous extract (10%), with those being indicated for the biological control of pest insects. |
id |
IBIO-1_c0e84ba05baa3b0a065586f4878506b0 |
---|---|
oai_identifier_str |
oai:scielo:S1808-16572012000400007 |
network_acronym_str |
IBIO-1 |
network_name_str |
Arquivos do instituto biológico (Online) |
repository_id_str |
|
spelling |
Toxicity intraperitoneal and intragastric route of Bacillus thuringiensis and Melia azedarach in miceBacteriummammalianMeliaceaetoxicologyThe aim of this investigation was the assessment of toxicity of two new isolates of Bacillus thuringiensis, and the aqueous extract of Melia azedarach through in vivo assays in CF1 mice. Bt 1958-2, Bt 2014-2 and the BTh Thuricide 63 standard isolates were grown in liquid usual glicosed medium, and Cry proteins were purified by centrifugation on a sucrose gradient. The supernatant was autoclaved at 121º C, 15min. to maintain the exotoxins. Dehydrated leaves of M. azedarach were used to prepare a 10% aqueous extract. Mice were treated either orally or intraperitoneally with a whole bacterial suspension (1.10(10) UFC/mL), a culture supernatant or purified crystal protein (50 µg/mL), and with the plant extract (50 µg/mL). The stomachs of the mice were collected and observed in stereomicroscopy, and the stomach contents were analyzed in 10% SDS-PAGE. Results showed that none of the oral treatments were toxic to mice, but intraperitoneal bacterial suspensions were lethal to the animals 6 - 24 hours after injection. In conclusion, the Cry proteins of the new B. thuringiensis isolates must be evaluated for their use as tools in the biotechnology field, since they do not show toxicity against mammals, intragastrically or peritoneally, just like the M. azedarach aqueous extract (10%), with those being indicated for the biological control of pest insects.Instituto Biológico2012-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1808-16572012000400007Arquivos do Instituto Biológico v.79 n.4 2012reponame:Arquivos do instituto biológico (Online)instname:Instituto Biológico (IB)instacron:IBIO10.1590/S1808-16572012000400007info:eu-repo/semantics/openAccessBerlitz,D.L.Giovenardi,M.Charles,J.-F.Fiúza,L.M.eng2013-06-03T00:00:00Zoai:scielo:S1808-16572012000400007Revistahttp://www.biologico.sp.gov.br/arquivos_bio.phphttps://old.scielo.br/oai/scielo-oai.php||arquivos@biologico.sp.gov.br1808-16570020-3653opendoar:2013-06-03T00:00Arquivos do instituto biológico (Online) - Instituto Biológico (IB)false |
dc.title.none.fl_str_mv |
Toxicity intraperitoneal and intragastric route of Bacillus thuringiensis and Melia azedarach in mice |
title |
Toxicity intraperitoneal and intragastric route of Bacillus thuringiensis and Melia azedarach in mice |
spellingShingle |
Toxicity intraperitoneal and intragastric route of Bacillus thuringiensis and Melia azedarach in mice Berlitz,D.L. Bacterium mammalian Meliaceae toxicology |
title_short |
Toxicity intraperitoneal and intragastric route of Bacillus thuringiensis and Melia azedarach in mice |
title_full |
Toxicity intraperitoneal and intragastric route of Bacillus thuringiensis and Melia azedarach in mice |
title_fullStr |
Toxicity intraperitoneal and intragastric route of Bacillus thuringiensis and Melia azedarach in mice |
title_full_unstemmed |
Toxicity intraperitoneal and intragastric route of Bacillus thuringiensis and Melia azedarach in mice |
title_sort |
Toxicity intraperitoneal and intragastric route of Bacillus thuringiensis and Melia azedarach in mice |
author |
Berlitz,D.L. |
author_facet |
Berlitz,D.L. Giovenardi,M. Charles,J.-F. Fiúza,L.M. |
author_role |
author |
author2 |
Giovenardi,M. Charles,J.-F. Fiúza,L.M. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Berlitz,D.L. Giovenardi,M. Charles,J.-F. Fiúza,L.M. |
dc.subject.por.fl_str_mv |
Bacterium mammalian Meliaceae toxicology |
topic |
Bacterium mammalian Meliaceae toxicology |
description |
The aim of this investigation was the assessment of toxicity of two new isolates of Bacillus thuringiensis, and the aqueous extract of Melia azedarach through in vivo assays in CF1 mice. Bt 1958-2, Bt 2014-2 and the BTh Thuricide 63 standard isolates were grown in liquid usual glicosed medium, and Cry proteins were purified by centrifugation on a sucrose gradient. The supernatant was autoclaved at 121º C, 15min. to maintain the exotoxins. Dehydrated leaves of M. azedarach were used to prepare a 10% aqueous extract. Mice were treated either orally or intraperitoneally with a whole bacterial suspension (1.10(10) UFC/mL), a culture supernatant or purified crystal protein (50 µg/mL), and with the plant extract (50 µg/mL). The stomachs of the mice were collected and observed in stereomicroscopy, and the stomach contents were analyzed in 10% SDS-PAGE. Results showed that none of the oral treatments were toxic to mice, but intraperitoneal bacterial suspensions were lethal to the animals 6 - 24 hours after injection. In conclusion, the Cry proteins of the new B. thuringiensis isolates must be evaluated for their use as tools in the biotechnology field, since they do not show toxicity against mammals, intragastrically or peritoneally, just like the M. azedarach aqueous extract (10%), with those being indicated for the biological control of pest insects. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1808-16572012000400007 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1808-16572012000400007 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1808-16572012000400007 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Biológico |
publisher.none.fl_str_mv |
Instituto Biológico |
dc.source.none.fl_str_mv |
Arquivos do Instituto Biológico v.79 n.4 2012 reponame:Arquivos do instituto biológico (Online) instname:Instituto Biológico (IB) instacron:IBIO |
instname_str |
Instituto Biológico (IB) |
instacron_str |
IBIO |
institution |
IBIO |
reponame_str |
Arquivos do instituto biológico (Online) |
collection |
Arquivos do instituto biológico (Online) |
repository.name.fl_str_mv |
Arquivos do instituto biológico (Online) - Instituto Biológico (IB) |
repository.mail.fl_str_mv |
||arquivos@biologico.sp.gov.br |
_version_ |
1754193668668915712 |