A procedure for correcting the position of plant tissue samples in accidentally polymerized glycol methacrylate resin

Detalhes bibliográficos
Autor(a) principal: Leitão,Carlos André Espolador
Data de Publicação: 2019
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Hoehnea
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2236-89062019000100204
Resumo: ABSTRACT Glycol methacrylate resin is a very convenient medium used for inclusions of various types of tissue for histological and cytological studies. It provides excellent mechanical support in the sectioning of samples, even the ones with big differences in hardness. Additionally, it allows a large range of stain techniques and histochemical tests. During the infiltration stage, especially if it lasts for several days, the resin may be accidentally polymerized, which may give the impression that the sample has been lost. The present work aims to provide a technique for correcting the position of plant tissues samples embedded in accidentally polymerized glycol methacrylate resin. For this purpose, blocks of resin accidentally polymerized containing plant samples were trimmed and reshaped appropriately. The results were satisfactory and it was possible to obtain good sections of three micrometers thick.
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spelling A procedure for correcting the position of plant tissue samples in accidentally polymerized glycol methacrylate resinembeddinghistologyhistoresinplant anatomytechnique in microscopyABSTRACT Glycol methacrylate resin is a very convenient medium used for inclusions of various types of tissue for histological and cytological studies. It provides excellent mechanical support in the sectioning of samples, even the ones with big differences in hardness. Additionally, it allows a large range of stain techniques and histochemical tests. During the infiltration stage, especially if it lasts for several days, the resin may be accidentally polymerized, which may give the impression that the sample has been lost. The present work aims to provide a technique for correcting the position of plant tissues samples embedded in accidentally polymerized glycol methacrylate resin. For this purpose, blocks of resin accidentally polymerized containing plant samples were trimmed and reshaped appropriately. The results were satisfactory and it was possible to obtain good sections of three micrometers thick.Instituto de Pesquisas Ambientais2019-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S2236-89062019000100204Hoehnea v.46 n.1 2019reponame:Hoehneainstname:Instituto de Botânica (IBT)instacron:IBT10.1590/2236-8906-69/2017info:eu-repo/semantics/openAccessLeitão,Carlos André Espoladoreng2019-03-29T00:00:00Zoai:scielo:S2236-89062019000100204Revistahttp://www.ibot.sp.gov.br/publicacoes/hoehnea/sobre_hoehnea.phpPUBhttps://old.scielo.br/oai/scielo-oai.php||hoehneaibt@gmail.com2236-89060073-2877opendoar:2019-03-29T00:00Hoehnea - Instituto de Botânica (IBT)false
dc.title.none.fl_str_mv A procedure for correcting the position of plant tissue samples in accidentally polymerized glycol methacrylate resin
title A procedure for correcting the position of plant tissue samples in accidentally polymerized glycol methacrylate resin
spellingShingle A procedure for correcting the position of plant tissue samples in accidentally polymerized glycol methacrylate resin
Leitão,Carlos André Espolador
embedding
histology
historesin
plant anatomy
technique in microscopy
title_short A procedure for correcting the position of plant tissue samples in accidentally polymerized glycol methacrylate resin
title_full A procedure for correcting the position of plant tissue samples in accidentally polymerized glycol methacrylate resin
title_fullStr A procedure for correcting the position of plant tissue samples in accidentally polymerized glycol methacrylate resin
title_full_unstemmed A procedure for correcting the position of plant tissue samples in accidentally polymerized glycol methacrylate resin
title_sort A procedure for correcting the position of plant tissue samples in accidentally polymerized glycol methacrylate resin
author Leitão,Carlos André Espolador
author_facet Leitão,Carlos André Espolador
author_role author
dc.contributor.author.fl_str_mv Leitão,Carlos André Espolador
dc.subject.por.fl_str_mv embedding
histology
historesin
plant anatomy
technique in microscopy
topic embedding
histology
historesin
plant anatomy
technique in microscopy
description ABSTRACT Glycol methacrylate resin is a very convenient medium used for inclusions of various types of tissue for histological and cytological studies. It provides excellent mechanical support in the sectioning of samples, even the ones with big differences in hardness. Additionally, it allows a large range of stain techniques and histochemical tests. During the infiltration stage, especially if it lasts for several days, the resin may be accidentally polymerized, which may give the impression that the sample has been lost. The present work aims to provide a technique for correcting the position of plant tissues samples embedded in accidentally polymerized glycol methacrylate resin. For this purpose, blocks of resin accidentally polymerized containing plant samples were trimmed and reshaped appropriately. The results were satisfactory and it was possible to obtain good sections of three micrometers thick.
publishDate 2019
dc.date.none.fl_str_mv 2019-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2236-89062019000100204
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2236-89062019000100204
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/2236-8906-69/2017
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Pesquisas Ambientais
publisher.none.fl_str_mv Instituto de Pesquisas Ambientais
dc.source.none.fl_str_mv Hoehnea v.46 n.1 2019
reponame:Hoehnea
instname:Instituto de Botânica (IBT)
instacron:IBT
instname_str Instituto de Botânica (IBT)
instacron_str IBT
institution IBT
reponame_str Hoehnea
collection Hoehnea
repository.name.fl_str_mv Hoehnea - Instituto de Botânica (IBT)
repository.mail.fl_str_mv ||hoehneaibt@gmail.com
_version_ 1754193648028745728

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