Factors involved in the apoptotic cell death mechanism in yellow fever hepatitis
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/4577 |
Resumo: | Yellow fever (YF), a non-contagious infectious disease, is endemic or enzootic to the tropical regions of the Americas and Africa. Periodic outbreaks or epidemics have a significant impact on public health. Programmed cell death, or apoptosis, is generally characterised by distinct morphological changes and energy-dependent biochemical pathways. In this study, we performed immunohistochemistry analysis to identify and quantify proteases and protein targets involved in the cascade that triggers apoptosis in YF virus (YFV)-infected human hepatocytes. Liver tissue samples were collected from 26 individuals, among whom 21 were diagnosed as YF-positive, and five were flavivirus-negative and died due to other causes. The histopathological alterations in YFV-positive cases were characterised by the presence of apoptotic bodies, steatosis, cellular swelling, and extensive necrosis and haemorrhage in the hepatic lobules. Additionally, we observed an abundance of inflammatory infiltrates in the portal tract. The expression of various apoptotic markers in the hepatic parenchyma, including CASPASE 3, CASPASE 8, BAX, FAS, FASL, GRANZYME B, and SURVIVIN, differed between YFV-positive cases and controls. Collectively, this study confirmed the complexity of YFV infection-induced apoptosis in situ. However, our data suggest that apoptosis in liver parenchyma lesions may significantly contribute to the pathogenesis of fatal YF in humans. |
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Lopes, Jeferson da CostaFalcão, Luiz Fábio MagnoMartins Filho, Arnaldo JorgeCarvalho, Marcos Luiz GaiaMendes, Caio Cesar HenriquesOlímpio, Fábio AlvesMiranda, Vanessa do Socorro CabralSantos, Lais Carneiro dosChiang, Jannifer OliveiraCruz, Ana Cecília RibeiroGalúcio, Vanessa Costa AlvesAzevedo, Raimunda do Socorro da SilvaMartins, Lívia CaricioDuarte, Maria Irma SeixasSousa, Jorge Rodrigues deVasconcelos, Pedro Fernando da CostaQuaresma, Juarez Antônio Simões2022-07-04T14:11:36Z2022-07-04T14:11:36Z2022LOPES, Jeferson da Costa et al. Factors involved in the apoptotic cell death mechanism in yellow fever hepatitis. Viruses, v. 14, n. 6, p. 1-10, June 2022.1999-4915https://patua.iec.gov.br/handle/iec/457710.3390/v14061204Yellow fever (YF), a non-contagious infectious disease, is endemic or enzootic to the tropical regions of the Americas and Africa. Periodic outbreaks or epidemics have a significant impact on public health. Programmed cell death, or apoptosis, is generally characterised by distinct morphological changes and energy-dependent biochemical pathways. In this study, we performed immunohistochemistry analysis to identify and quantify proteases and protein targets involved in the cascade that triggers apoptosis in YF virus (YFV)-infected human hepatocytes. Liver tissue samples were collected from 26 individuals, among whom 21 were diagnosed as YF-positive, and five were flavivirus-negative and died due to other causes. The histopathological alterations in YFV-positive cases were characterised by the presence of apoptotic bodies, steatosis, cellular swelling, and extensive necrosis and haemorrhage in the hepatic lobules. Additionally, we observed an abundance of inflammatory infiltrates in the portal tract. The expression of various apoptotic markers in the hepatic parenchyma, including CASPASE 3, CASPASE 8, BAX, FAS, FASL, GRANZYME B, and SURVIVIN, differed between YFV-positive cases and controls. Collectively, this study confirmed the complexity of YFV infection-induced apoptosis in situ. However, our data suggest that apoptosis in liver parenchyma lesions may significantly contribute to the pathogenesis of fatal YF in humans.Brazilian Council of Scientific and Technological Development Agency for financial support to PFCV (grants 457664/2013-4 and 303999/2016-0Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, BrasilState University of Para. Department of Pathology. Belem. PA, BrazilMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, BrasilMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, BrasilState University of Para. Department of Pathology. Belem. PA, BrazilFederal University of Para. Tripical Medicine Unit. Belem, PA, BrazilMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, BrasilMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, BrasilMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, BrasilMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, BrasilState University of Para. Department of Pathology. Belem. PA, BrazilMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, BrasilMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, BrasilSao Paulo University. Schhol of Medicine. Sao Paulo, SP, BrazilMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil / State University of Para. Department of Pathology. Belem. PA, BrazilState University of Para. Department of Pathology. Belem. PA, Brazil /State University of Para. Department of Pathology. Belem. PA, Brazil / Federal University of Para. Tripical Medicine Unit. Belem, PA, Brazil / Sao Paulo University. Schhol of Medicine. Sao Paulo, SP, BrazilengMDPIFactors involved in the apoptotic cell death mechanism in yellow fever hepatitisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFebre Amarela / patologiaApoptoseProteínas Reguladoras de ApoptoseHepatite / patologiaMorte Celular / imunologiainfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALFactors involved in the apoptotic cell death mechanism in yellow fever hepatitis.pdfFactors involved in the apoptotic cell death mechanism in yellow fever hepatitis.pdfapplication/pdf5667018https://patua.iec.gov.br/bitstreams/4ee7e2b1-b319-4236-b587-98d6da85f5aa/downloadca269710bc84ac983c4639089b442cddMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82182https://patua.iec.gov.br/bitstreams/ef0db514-729d-448b-9306-ff859a2b8fb9/download11832eea31b16df8613079d742d61793MD52TEXTFactors involved in the apoptotic cell death mechanism in yellow fever hepatitis.pdf.txtFactors involved in the apoptotic cell death mechanism in yellow fever hepatitis.pdf.txtExtracted texttext/plain40349https://patua.iec.gov.br/bitstreams/b7418cf6-155e-441f-9177-aac5af78d042/download04c48f83ba100d63e2543ac0e8fb20bbMD55THUMBNAILFactors involved in the apoptotic cell death mechanism in yellow fever hepatitis.pdf.jpgFactors involved in the apoptotic cell death mechanism in yellow fever hepatitis.pdf.jpgGenerated Thumbnailimage/jpeg5821https://patua.iec.gov.br/bitstreams/1c55503f-656a-4907-bd52-f39afc67a255/downloade0c610f8285606615ac19b70aefc62d8MD56iec/45772022-10-21 00:06:24.896oai:patua.iec.gov.br:iec/4577https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-21T00:06:24Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)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 |
dc.title.pt_BR.fl_str_mv |
Factors involved in the apoptotic cell death mechanism in yellow fever hepatitis |
title |
Factors involved in the apoptotic cell death mechanism in yellow fever hepatitis |
spellingShingle |
Factors involved in the apoptotic cell death mechanism in yellow fever hepatitis Lopes, Jeferson da Costa Febre Amarela / patologia Apoptose Proteínas Reguladoras de Apoptose Hepatite / patologia Morte Celular / imunologia |
title_short |
Factors involved in the apoptotic cell death mechanism in yellow fever hepatitis |
title_full |
Factors involved in the apoptotic cell death mechanism in yellow fever hepatitis |
title_fullStr |
Factors involved in the apoptotic cell death mechanism in yellow fever hepatitis |
title_full_unstemmed |
Factors involved in the apoptotic cell death mechanism in yellow fever hepatitis |
title_sort |
Factors involved in the apoptotic cell death mechanism in yellow fever hepatitis |
author |
Lopes, Jeferson da Costa |
author_facet |
Lopes, Jeferson da Costa Falcão, Luiz Fábio Magno Martins Filho, Arnaldo Jorge Carvalho, Marcos Luiz Gaia Mendes, Caio Cesar Henriques Olímpio, Fábio Alves Miranda, Vanessa do Socorro Cabral Santos, Lais Carneiro dos Chiang, Jannifer Oliveira Cruz, Ana Cecília Ribeiro Galúcio, Vanessa Costa Alves Azevedo, Raimunda do Socorro da Silva Martins, Lívia Caricio Duarte, Maria Irma Seixas Sousa, Jorge Rodrigues de Vasconcelos, Pedro Fernando da Costa Quaresma, Juarez Antônio Simões |
author_role |
author |
author2 |
Falcão, Luiz Fábio Magno Martins Filho, Arnaldo Jorge Carvalho, Marcos Luiz Gaia Mendes, Caio Cesar Henriques Olímpio, Fábio Alves Miranda, Vanessa do Socorro Cabral Santos, Lais Carneiro dos Chiang, Jannifer Oliveira Cruz, Ana Cecília Ribeiro Galúcio, Vanessa Costa Alves Azevedo, Raimunda do Socorro da Silva Martins, Lívia Caricio Duarte, Maria Irma Seixas Sousa, Jorge Rodrigues de Vasconcelos, Pedro Fernando da Costa Quaresma, Juarez Antônio Simões |
author2_role |
author author author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Lopes, Jeferson da Costa Falcão, Luiz Fábio Magno Martins Filho, Arnaldo Jorge Carvalho, Marcos Luiz Gaia Mendes, Caio Cesar Henriques Olímpio, Fábio Alves Miranda, Vanessa do Socorro Cabral Santos, Lais Carneiro dos Chiang, Jannifer Oliveira Cruz, Ana Cecília Ribeiro Galúcio, Vanessa Costa Alves Azevedo, Raimunda do Socorro da Silva Martins, Lívia Caricio Duarte, Maria Irma Seixas Sousa, Jorge Rodrigues de Vasconcelos, Pedro Fernando da Costa Quaresma, Juarez Antônio Simões |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Febre Amarela / patologia Apoptose Proteínas Reguladoras de Apoptose Hepatite / patologia Morte Celular / imunologia |
topic |
Febre Amarela / patologia Apoptose Proteínas Reguladoras de Apoptose Hepatite / patologia Morte Celular / imunologia |
description |
Yellow fever (YF), a non-contagious infectious disease, is endemic or enzootic to the tropical regions of the Americas and Africa. Periodic outbreaks or epidemics have a significant impact on public health. Programmed cell death, or apoptosis, is generally characterised by distinct morphological changes and energy-dependent biochemical pathways. In this study, we performed immunohistochemistry analysis to identify and quantify proteases and protein targets involved in the cascade that triggers apoptosis in YF virus (YFV)-infected human hepatocytes. Liver tissue samples were collected from 26 individuals, among whom 21 were diagnosed as YF-positive, and five were flavivirus-negative and died due to other causes. The histopathological alterations in YFV-positive cases were characterised by the presence of apoptotic bodies, steatosis, cellular swelling, and extensive necrosis and haemorrhage in the hepatic lobules. Additionally, we observed an abundance of inflammatory infiltrates in the portal tract. The expression of various apoptotic markers in the hepatic parenchyma, including CASPASE 3, CASPASE 8, BAX, FAS, FASL, GRANZYME B, and SURVIVIN, differed between YFV-positive cases and controls. Collectively, this study confirmed the complexity of YFV infection-induced apoptosis in situ. However, our data suggest that apoptosis in liver parenchyma lesions may significantly contribute to the pathogenesis of fatal YF in humans. |
publishDate |
2022 |
dc.date.accessioned.fl_str_mv |
2022-07-04T14:11:36Z |
dc.date.available.fl_str_mv |
2022-07-04T14:11:36Z |
dc.date.issued.fl_str_mv |
2022 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
LOPES, Jeferson da Costa et al. Factors involved in the apoptotic cell death mechanism in yellow fever hepatitis. Viruses, v. 14, n. 6, p. 1-10, June 2022. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/4577 |
dc.identifier.issn.-.fl_str_mv |
1999-4915 |
dc.identifier.doi.-.fl_str_mv |
10.3390/v14061204 |
identifier_str_mv |
LOPES, Jeferson da Costa et al. Factors involved in the apoptotic cell death mechanism in yellow fever hepatitis. Viruses, v. 14, n. 6, p. 1-10, June 2022. 1999-4915 10.3390/v14061204 |
url |
https://patua.iec.gov.br/handle/iec/4577 |
dc.language.iso.fl_str_mv |
eng |
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eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
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collection |
Repositório Digital do Instituto Evandro Chagas (Patuá) |
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