Simultaneous occurrence of Epstein-Barr virus (EBV) in periodontal pockets and in oral squamous cell carcinoma: a cross-sectional study

• Autor(a) principal: Santos, Humberto Jácome
• Data de Publicação: 2021
• Outros Autores: Silva, Naira da Silva e, Resende, Renata Gonçalves, Pinheiro, Helder Henrique Costa, Machado, Luiz Fernando Almeida, Silva, Guilherme de Souza, Costa, Fernando de Oliveira, Costa, Igor Brasil, Alves, Ana Cláudia Braga Amoras, Mesquita, Ricardo Alves, Alves Júnior, Sérgio de Melo
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
• DOI: 10.1007/s00784-021-04258-z
• Texto Completo: https://patua.iec.gov.br/handle/iec/4478
• topic: Periodontite / virologia; Herpesvirus Humano 4; Infecções por Vírus Epstein-Barr / virologia; Neoplasias Bucais; Bolsa Periodontal

Objectives: This study aimed to investigate the detection of Epstein-Barr virus (EBV) in oral squamous cell carcinoma (OSCC) and to verify the concordance of EBV-DNA frequency in subgingival sites and in the OSCC. Methods: A cross-sectional study with 30 OSCC patients, aged from 44 to 88 years old, was conducted. Samples were collected in subgingival sites and at the OSCC, then submitted to DNA isolation, qPCR, and genotyping. Descriptive statistic was performed to report the frequency of EBV-DNA in all samples, and McNemar test was applied to verify the concordance among the EBV-DNA frequency in both sites. Results: The individuals presented 62 years old in average, and the majority were male (66.6%). EBV-DNA was detected in 56.7% OSCC lesions. Among the subgroup of 19 dentate individuals, high concordance (73.7%) in both EBV-DNA detection and the absence in subgingival sites and OSCC was observed, and it was statistically significant (p < 0.05). Conclusions: We report the notable occurrence of EBV-DNA in OSCC; also, the presence of EBV in periodontal sites may contribute to find it in OSCC, although the possible contribution of EBV in the OSCC remains to be investigated.