Rotavirus analyses by SYBR Green real-time PCR and microbiological contamination in bivalves cultivated in coastal water of Amazonian Brazil

Detalhes bibliográficos
Autor(a) principal: Marinho, A. N. R
Data de Publicação: 2018
Outros Autores: Rocha, D. C. C, Kanai, Y. K, Alves, C. M, Costa, D. C, Sousa, A. H, Barros, B. C. V, Bonfim, M. C. M. S, Mascarenhas, Joana D'Arc Pereira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
Texto Completo: https://patua.iec.gov.br/handle/iec/3600
Resumo: The contamination of mussels and oysters by viruses and bacteria is often associated with water contamination and gastroenteritis in humans. The present study evaluated viral and bacterial contamination in 380 samples, from nine mollusk-producing regions in coastal water north of the Brazilian Amazon. Rotavirus contamination was studied for groups A to H, using a two-step SYBR Green RT-qPCR (quantitative reverse transcription polymerase chain reaction), and bacterial families Enterobacteriaceae, Vibrionaceae, and Aeromonadaceae by classical and molecular methods. From the 19 pools analyzed, 26.3% (5/19) were positive for group A Rotavirus, I2 genotype for VP6 region, without amplifications for groups B–H. Bacteriological analysis identified Escherichia coli isolates in 89.5% (17/19) with identification of atypical enteropathogenic E. coli aEPEC in 10.5% (2/19), Salmonella (Groups C1 and G) (10.5%, 2/19), Vibrio alginolyticus (57.9%, 11/19) V. parahaemolyticus (63.2%, 12/19), V. fluvialis (42.1%, 8/19), V. vulnificus (10.5%, 2/19), V. cholerae non-O1, non O139(10.5%, 2/19) and Aeromonas salmonicida (52.6%, 10/19). All the samples investigated presented some level of contamination by enterobacteria, rotavirus, or both, and these results may reflect the level of contamination in the Northern Amazon Region, due to the natural maintenance of some of these agents or by the proximity with human populations and their sewer.
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spelling Marinho, A. N. RRocha, D. C. CKanai, Y. KAlves, C. MCosta, D. CSousa, A. HBarros, B. C. VBonfim, M. C. M. SMascarenhas, Joana D'Arc Pereira2019-02-20T18:00:29Z2019-02-20T18:00:29Z2018MARINHO, A. N. R. et al. Rotavirus analyses by SYBR Green real-time PCR and microbiological contamination in bivalves cultivated in coastal water of Amazonian Brazil. Journal of Water and Health, v. 16, n. 6 , p. 970-979, Dec. 2018.1477-8920https://patua.iec.gov.br/handle/iec/360010.2166/wh.2018.130The contamination of mussels and oysters by viruses and bacteria is often associated with water contamination and gastroenteritis in humans. The present study evaluated viral and bacterial contamination in 380 samples, from nine mollusk-producing regions in coastal water north of the Brazilian Amazon. Rotavirus contamination was studied for groups A to H, using a two-step SYBR Green RT-qPCR (quantitative reverse transcription polymerase chain reaction), and bacterial families Enterobacteriaceae, Vibrionaceae, and Aeromonadaceae by classical and molecular methods. From the 19 pools analyzed, 26.3% (5/19) were positive for group A Rotavirus, I2 genotype for VP6 region, without amplifications for groups B–H. Bacteriological analysis identified Escherichia coli isolates in 89.5% (17/19) with identification of atypical enteropathogenic E. coli aEPEC in 10.5% (2/19), Salmonella (Groups C1 and G) (10.5%, 2/19), Vibrio alginolyticus (57.9%, 11/19) V. parahaemolyticus (63.2%, 12/19), V. fluvialis (42.1%, 8/19), V. vulnificus (10.5%, 2/19), V. cholerae non-O1, non O139(10.5%, 2/19) and Aeromonas salmonicida (52.6%, 10/19). All the samples investigated presented some level of contamination by enterobacteria, rotavirus, or both, and these results may reflect the level of contamination in the Northern Amazon Region, due to the natural maintenance of some of these agents or by the proximity with human populations and their sewer.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.engIWA PublishingRotavirus analyses by SYBR Green real-time PCR and microbiological contamination in bivalves cultivated in coastal water of Amazonian Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleÁgua Costeira / análisePoluição Costeira / análiseBivalves / crescimento & desenvolvimentoAnálise MicrobiológicaContaminação de AlimentosMicrorganismos Aquáticos / análiseReação em Cadeia da Polimerase em Tempo Real / métodosRegião Norte (BR)Amazônia Brasileirainfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALRotavirus analyses by SYBR Green real-time PCR and microbiological contamination in bivalves cultivated in coastal water of Amazonian Brazil.pdfRotavirus analyses by SYBR Green real-time PCR and microbiological contamination in bivalves cultivated in coastal water of Amazonian Brazil.pdfapplication/pdf624050https://patua.iec.gov.br/bitstreams/fcf2d61d-d006-4a7d-8bd2-b88391614d70/download00f51b8af5800f9c24e04bcdccaf9e65MD51TEXTRotavirus analyses by SYBR Green real-time PCR and microbiological contamination in bivalves cultivated in coastal water of Amazonian Brazil.pdf.txtRotavirus analyses by SYBR Green real-time PCR and microbiological contamination in bivalves cultivated in coastal water of Amazonian Brazil.pdf.txtExtracted texttext/plain34609https://patua.iec.gov.br/bitstreams/b31cd4ac-13d1-4843-a13c-b4d82500395c/download31655c8eb9edf1a49a45a9934d5a0c7eMD55THUMBNAILRotavirus analyses by SYBR Green real-time PCR and microbiological contamination in bivalves cultivated in coastal water of Amazonian Brazil.pdf.jpgRotavirus analyses by SYBR Green real-time PCR and microbiological contamination in bivalves cultivated in coastal water of Amazonian Brazil.pdf.jpgGenerated Thumbnailimage/jpeg5372https://patua.iec.gov.br/bitstreams/4dd3d3ea-8f3f-4858-a73f-7372816990e7/download7f8a579265be0dbe1479622c7a9f7a1aMD56LICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv Rotavirus analyses by SYBR Green real-time PCR and microbiological contamination in bivalves cultivated in coastal water of Amazonian Brazil
title Rotavirus analyses by SYBR Green real-time PCR and microbiological contamination in bivalves cultivated in coastal water of Amazonian Brazil
spellingShingle Rotavirus analyses by SYBR Green real-time PCR and microbiological contamination in bivalves cultivated in coastal water of Amazonian Brazil
Marinho, A. N. R
Água Costeira / análise
Poluição Costeira / análise
Bivalves / crescimento & desenvolvimento
Análise Microbiológica
Contaminação de Alimentos
Microrganismos Aquáticos / análise
Reação em Cadeia da Polimerase em Tempo Real / métodos
Região Norte (BR)
Amazônia Brasileira
title_short Rotavirus analyses by SYBR Green real-time PCR and microbiological contamination in bivalves cultivated in coastal water of Amazonian Brazil
title_full Rotavirus analyses by SYBR Green real-time PCR and microbiological contamination in bivalves cultivated in coastal water of Amazonian Brazil
title_fullStr Rotavirus analyses by SYBR Green real-time PCR and microbiological contamination in bivalves cultivated in coastal water of Amazonian Brazil
title_full_unstemmed Rotavirus analyses by SYBR Green real-time PCR and microbiological contamination in bivalves cultivated in coastal water of Amazonian Brazil
title_sort Rotavirus analyses by SYBR Green real-time PCR and microbiological contamination in bivalves cultivated in coastal water of Amazonian Brazil
author Marinho, A. N. R
author_facet Marinho, A. N. R
Rocha, D. C. C
Kanai, Y. K
Alves, C. M
Costa, D. C
Sousa, A. H
Barros, B. C. V
Bonfim, M. C. M. S
Mascarenhas, Joana D'Arc Pereira
author_role author
author2 Rocha, D. C. C
Kanai, Y. K
Alves, C. M
Costa, D. C
Sousa, A. H
Barros, B. C. V
Bonfim, M. C. M. S
Mascarenhas, Joana D'Arc Pereira
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Marinho, A. N. R
Rocha, D. C. C
Kanai, Y. K
Alves, C. M
Costa, D. C
Sousa, A. H
Barros, B. C. V
Bonfim, M. C. M. S
Mascarenhas, Joana D'Arc Pereira
dc.subject.decsPrimary.pt_BR.fl_str_mv Água Costeira / análise
Poluição Costeira / análise
Bivalves / crescimento & desenvolvimento
Análise Microbiológica
Contaminação de Alimentos
Microrganismos Aquáticos / análise
Reação em Cadeia da Polimerase em Tempo Real / métodos
Região Norte (BR)
Amazônia Brasileira
topic Água Costeira / análise
Poluição Costeira / análise
Bivalves / crescimento & desenvolvimento
Análise Microbiológica
Contaminação de Alimentos
Microrganismos Aquáticos / análise
Reação em Cadeia da Polimerase em Tempo Real / métodos
Região Norte (BR)
Amazônia Brasileira
description The contamination of mussels and oysters by viruses and bacteria is often associated with water contamination and gastroenteritis in humans. The present study evaluated viral and bacterial contamination in 380 samples, from nine mollusk-producing regions in coastal water north of the Brazilian Amazon. Rotavirus contamination was studied for groups A to H, using a two-step SYBR Green RT-qPCR (quantitative reverse transcription polymerase chain reaction), and bacterial families Enterobacteriaceae, Vibrionaceae, and Aeromonadaceae by classical and molecular methods. From the 19 pools analyzed, 26.3% (5/19) were positive for group A Rotavirus, I2 genotype for VP6 region, without amplifications for groups B–H. Bacteriological analysis identified Escherichia coli isolates in 89.5% (17/19) with identification of atypical enteropathogenic E. coli aEPEC in 10.5% (2/19), Salmonella (Groups C1 and G) (10.5%, 2/19), Vibrio alginolyticus (57.9%, 11/19) V. parahaemolyticus (63.2%, 12/19), V. fluvialis (42.1%, 8/19), V. vulnificus (10.5%, 2/19), V. cholerae non-O1, non O139(10.5%, 2/19) and Aeromonas salmonicida (52.6%, 10/19). All the samples investigated presented some level of contamination by enterobacteria, rotavirus, or both, and these results may reflect the level of contamination in the Northern Amazon Region, due to the natural maintenance of some of these agents or by the proximity with human populations and their sewer.
publishDate 2018
dc.date.issued.fl_str_mv 2018
dc.date.accessioned.fl_str_mv 2019-02-20T18:00:29Z
dc.date.available.fl_str_mv 2019-02-20T18:00:29Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MARINHO, A. N. R. et al. Rotavirus analyses by SYBR Green real-time PCR and microbiological contamination in bivalves cultivated in coastal water of Amazonian Brazil. Journal of Water and Health, v. 16, n. 6 , p. 970-979, Dec. 2018.
dc.identifier.uri.fl_str_mv https://patua.iec.gov.br/handle/iec/3600
dc.identifier.issn.-.fl_str_mv 1477-8920
dc.identifier.doi.-.fl_str_mv 10.2166/wh.2018.130
identifier_str_mv MARINHO, A. N. R. et al. Rotavirus analyses by SYBR Green real-time PCR and microbiological contamination in bivalves cultivated in coastal water of Amazonian Brazil. Journal of Water and Health, v. 16, n. 6 , p. 970-979, Dec. 2018.
1477-8920
10.2166/wh.2018.130
url https://patua.iec.gov.br/handle/iec/3600
dc.language.iso.fl_str_mv eng
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dc.publisher.none.fl_str_mv IWA Publishing
publisher.none.fl_str_mv IWA Publishing
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