First isolation and genome sequence analysis of West Nile Virus in mosquitoes in Brazil

• Autor(a) principal: Nunes Neto, Joaquim Pinto
• Data de Publicação: 2023
• Outros Autores: Reis, Lúcia Aline Moura, Freitas, Maria Nazaré Oliveira, Nascimento, Bruna Laís Sena do, Chagas, Liliane Leal das, Costa, Hernan Hermes Monteiro da, Rodrigues, Jéssica Cecília Pinheiro, Braga, Camila Margalho, Silva, Eliana Vieira Pinto da, Silva, Sandro Patroca da, Martins, Lívia Caricio
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
• Texto Completo: https://patua.iec.gov.br/handle/iec/6817
• topic: Culex / anatomia & histologia; Vírus do Nilo Ocidental / isolamento & purificação; Febre do Nilo Ocidental / virologia; Vetores de Doenças

West Nile virus is a flavivirus transmitted by mosquitoes, mainly of the genus Culex. In Brazil, serological studies have already indicated the circulation of the virus since 2003, with the first human case detected in 2014. The objective of the present paper is to report the first isolation of WNV in a Culex (Melanoconion) mosquito. Arthropods were collected by protected human attraction and CDC light bait, and taxonomically identified and analyzed by viral isolation, complement fixation and genomic sequencing tests. WNV was isolated from samples of Culex (Melanoconion) mosquitoes, and the sequencing analysis demonstrated that the isolated strain belonged to lineage 1a. The finding of the present study presents the first evidence of the isolation and genome sequencing of WNV in arthropods in Brazil.