Changes in hippocampal astrocyte morphology of Ruddy turnstone (Arenaria interpres) during the wintering period at the mangroves of Amazon
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/4105 |
Resumo: | Astrocytes are essential for lipid neuronal metabolism in long-distance uninterrupted migratory flights, when glucose is not available as the main source of energy. We previously demonstrated in Calidris pusilla that after uninterrupted 5 days transatlantic flight, astrocytes shrink and reduce its number in the hippocampal formation. Here we shifted our attention to the wintering period and tested the hypothesis that hippocampal astrocyte morphology of A interpres will change as the wintering period progresses towards the premigration window. To that end we used Arenaria interpres, which also crosses the Atlantic Ocean and reaches the mangroves of the Amazon River estuary for wintering. Birds were captured in September/October (closer to the arrival in the coast of Bragança, Para, Brazil for wintering) and in April/May (closer to the departure towards the breeding sites) and had their brains processed for selective GFAP-astrocyte immunolabeling. Three-dimensional reconstructions of the immunostained astrocytes were performed and morphological classification was done based on hierarchical cluster and discriminant analysis of multimodal morphometric features. We found two morphological phenotypes of astrocytes in the newcomers which differentially increased its morphological complexities as wintering period progresses towards the pre-migration window. Taken together, our findings demonstrate that the long-distance non-stop flight and wintering period differentially affected the two astrocytes morphotypes, suggesting distinct physiological roles for these cells. We suggest that morphological changes during the wintering period, may be part of the adaptive plasticity of the local hippocampal circuits of A. interpres in preparation for the long journey back to their breeding sites in the north hemisphere. |
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Costa, Emanuel Ramos daHenrique, Ediely PereiraSilva, João Batista daPereira, Patrick Douglas CorrêaAbreu, Cintya Castro deFernandes, Taiany NogueiraMagalhães, Nara Gyzely de MoraisSilva, Anderson de Jesus Falcão daGuerreiro, Luma Cristina FerreiraDiniz, Cristovam GuerreiroDiniz, Cristovam Wanderley PicançoDiniz, Daniel Guerreiro2020-06-30T14:13:15Z2020-06-30T14:13:15Z2020COSTA, Emanuel Ramos da et al. Changes in hippocampal astrocyte morphology of Ruddy turnstone (Arenaria interpres) during the wintering period at the mangroves of Amazon. Journal of Chemical Neuroanatomy, v. 108, n. xx, 101805, Oct. 2020. DOI: https://doi.org/10.1016/j.jchemneu.2020.101805.1873-6300https://patua.iec.gov.br/handle/iec/410510.1016/j.jchemneu.2020.101805Astrocytes are essential for lipid neuronal metabolism in long-distance uninterrupted migratory flights, when glucose is not available as the main source of energy. We previously demonstrated in Calidris pusilla that after uninterrupted 5 days transatlantic flight, astrocytes shrink and reduce its number in the hippocampal formation. Here we shifted our attention to the wintering period and tested the hypothesis that hippocampal astrocyte morphology of A interpres will change as the wintering period progresses towards the premigration window. To that end we used Arenaria interpres, which also crosses the Atlantic Ocean and reaches the mangroves of the Amazon River estuary for wintering. Birds were captured in September/October (closer to the arrival in the coast of Bragança, Para, Brazil for wintering) and in April/May (closer to the departure towards the breeding sites) and had their brains processed for selective GFAP-astrocyte immunolabeling. Three-dimensional reconstructions of the immunostained astrocytes were performed and morphological classification was done based on hierarchical cluster and discriminant analysis of multimodal morphometric features. We found two morphological phenotypes of astrocytes in the newcomers which differentially increased its morphological complexities as wintering period progresses towards the pre-migration window. Taken together, our findings demonstrate that the long-distance non-stop flight and wintering period differentially affected the two astrocytes morphotypes, suggesting distinct physiological roles for these cells. We suggest that morphological changes during the wintering period, may be part of the adaptive plasticity of the local hippocampal circuits of A. interpres in preparation for the long journey back to their breeding sites in the north hemisphere.Universidade Federal do Pará. Instituto de Ciências Biológicas. Hospital Universitário João de Barros Barreto. Laboratório de Investigações em Neurodegeneração e Infecção. Belém, PA, Brazil.Instituto Federal de Educação. Ciência e Tecnologia do Pará. Laboratório de Biologia Molecular e Neuroecologia. Bragança, PA, Brazil.Instituto Federal de Educação. Ciência e Tecnologia do Pará. Laboratório de Biologia Molecular e Neuroecologia. Bragança, PA, Brazil.Instituto Federal de Educação. Ciência e Tecnologia do Pará. Laboratório de Biologia Molecular e Neuroecologia. Bragança, PA, Brazil.Instituto Federal de Educação. Ciência e Tecnologia do Pará. Laboratório de Biologia Molecular e Neuroecologia. Bragança, PA, Brazil.Instituto Federal de Educação. Ciência e Tecnologia do Pará. Laboratório de Biologia Molecular e Neuroecologia. Bragança, PA, Brazil.Instituto Federal de Educação. Ciência e Tecnologia do Pará. Laboratório de Biologia Molecular e Neuroecologia. Bragança, PA, Brazil.Instituto Federal de Educação. Ciência e Tecnologia do Pará. Laboratório de Biologia Molecular e Neuroecologia. Bragança, PA, Brazil.Universidade Federal do Pará. Instituto de Ciências Biológicas. Hospital Universitário João de Barros Barreto. Laboratório de Investigações em Neurodegeneração e Infecção. Belém, PA, Brazil.Instituto Federal de Educação. Ciência e Tecnologia do Pará. Laboratório de Biologia Molecular e Neuroecologia. Bragança, PA, Brazil.Universidade Federal do Pará. Instituto de Ciências Biológicas. Hospital Universitário João de Barros Barreto. Laboratório de Investigações em Neurodegeneração e Infecção. Belém, PA, Brazil.Universidade Federal do Pará. Instituto de Ciências Biológicas. Hospital Universitário João de Barros Barreto. Laboratório de Investigações em Neurodegeneração e Infecção. Belém, PA, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Microscopia Eletrônica. Belém, PA, Brasil.engElsevierChanges in hippocampal astrocyte morphology of Ruddy turnstone (Arenaria interpres) during the wintering period at the mangroves of Amazoninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCharadriiformes / anatomia & histologiaAvesSistema Nervoso / análiseAstrócitos / citologiaHipocampo / citologiaVoo AnimalRuddy turnstone (Arenaria interpres)info:eu-repo/semantics/embargoedAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECTEXTChanges in hippocampal astrocyte morphology of Ruddy turnstone (Arenaria interpres) during the wintering period at the mangroves of Amazon.pdf.txtChanges in hippocampal astrocyte morphology of Ruddy turnstone (Arenaria interpres) during the wintering period at the mangroves of Amazon.pdf.txtExtracted texttext/plain2https://patua.iec.gov.br/bitstreams/42d57398-69ef-4bf4-9f3e-4b20e85bebd1/downloade1c06d85ae7b8b032bef47e42e4c08f9MD55THUMBNAILChanges in hippocampal astrocyte morphology of Ruddy turnstone (Arenaria interpres) during the wintering period at the mangroves of Amazon.pdf.jpgChanges in hippocampal astrocyte morphology of Ruddy turnstone (Arenaria interpres) during the wintering period at the mangroves of Amazon.pdf.jpgGenerated Thumbnailimage/jpeg3095https://patua.iec.gov.br/bitstreams/7722db63-b5dd-4633-8439-d72f870db59d/download71859d578212107f7f8c49a4ce09d9eeMD56ORIGINALChanges in hippocampal astrocyte morphology of Ruddy turnstone (Arenaria interpres) during the wintering period at the mangroves of Amazon.pdfChanges in hippocampal astrocyte morphology of Ruddy turnstone (Arenaria interpres) during the wintering period at the mangroves of Amazon.pdfapplication/pdf551083https://patua.iec.gov.br/bitstreams/1efc4e43-059d-4e06-b52e-1f32fa47c7c7/downloadc9a9c128e29cac82a5d7fdf3f4e6da73MD51LICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv |
Changes in hippocampal astrocyte morphology of Ruddy turnstone (Arenaria interpres) during the wintering period at the mangroves of Amazon |
title |
Changes in hippocampal astrocyte morphology of Ruddy turnstone (Arenaria interpres) during the wintering period at the mangroves of Amazon |
spellingShingle |
Changes in hippocampal astrocyte morphology of Ruddy turnstone (Arenaria interpres) during the wintering period at the mangroves of Amazon Costa, Emanuel Ramos da Charadriiformes / anatomia & histologia Aves Sistema Nervoso / análise Astrócitos / citologia Hipocampo / citologia Voo Animal Ruddy turnstone (Arenaria interpres) |
title_short |
Changes in hippocampal astrocyte morphology of Ruddy turnstone (Arenaria interpres) during the wintering period at the mangroves of Amazon |
title_full |
Changes in hippocampal astrocyte morphology of Ruddy turnstone (Arenaria interpres) during the wintering period at the mangroves of Amazon |
title_fullStr |
Changes in hippocampal astrocyte morphology of Ruddy turnstone (Arenaria interpres) during the wintering period at the mangroves of Amazon |
title_full_unstemmed |
Changes in hippocampal astrocyte morphology of Ruddy turnstone (Arenaria interpres) during the wintering period at the mangroves of Amazon |
title_sort |
Changes in hippocampal astrocyte morphology of Ruddy turnstone (Arenaria interpres) during the wintering period at the mangroves of Amazon |
author |
Costa, Emanuel Ramos da |
author_facet |
Costa, Emanuel Ramos da Henrique, Ediely Pereira Silva, João Batista da Pereira, Patrick Douglas Corrêa Abreu, Cintya Castro de Fernandes, Taiany Nogueira Magalhães, Nara Gyzely de Morais Silva, Anderson de Jesus Falcão da Guerreiro, Luma Cristina Ferreira Diniz, Cristovam Guerreiro Diniz, Cristovam Wanderley Picanço Diniz, Daniel Guerreiro |
author_role |
author |
author2 |
Henrique, Ediely Pereira Silva, João Batista da Pereira, Patrick Douglas Corrêa Abreu, Cintya Castro de Fernandes, Taiany Nogueira Magalhães, Nara Gyzely de Morais Silva, Anderson de Jesus Falcão da Guerreiro, Luma Cristina Ferreira Diniz, Cristovam Guerreiro Diniz, Cristovam Wanderley Picanço Diniz, Daniel Guerreiro |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Costa, Emanuel Ramos da Henrique, Ediely Pereira Silva, João Batista da Pereira, Patrick Douglas Corrêa Abreu, Cintya Castro de Fernandes, Taiany Nogueira Magalhães, Nara Gyzely de Morais Silva, Anderson de Jesus Falcão da Guerreiro, Luma Cristina Ferreira Diniz, Cristovam Guerreiro Diniz, Cristovam Wanderley Picanço Diniz, Daniel Guerreiro |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Charadriiformes / anatomia & histologia Aves Sistema Nervoso / análise Astrócitos / citologia Hipocampo / citologia Voo Animal Ruddy turnstone (Arenaria interpres) |
topic |
Charadriiformes / anatomia & histologia Aves Sistema Nervoso / análise Astrócitos / citologia Hipocampo / citologia Voo Animal Ruddy turnstone (Arenaria interpres) |
description |
Astrocytes are essential for lipid neuronal metabolism in long-distance uninterrupted migratory flights, when glucose is not available as the main source of energy. We previously demonstrated in Calidris pusilla that after uninterrupted 5 days transatlantic flight, astrocytes shrink and reduce its number in the hippocampal formation. Here we shifted our attention to the wintering period and tested the hypothesis that hippocampal astrocyte morphology of A interpres will change as the wintering period progresses towards the premigration window. To that end we used Arenaria interpres, which also crosses the Atlantic Ocean and reaches the mangroves of the Amazon River estuary for wintering. Birds were captured in September/October (closer to the arrival in the coast of Bragança, Para, Brazil for wintering) and in April/May (closer to the departure towards the breeding sites) and had their brains processed for selective GFAP-astrocyte immunolabeling. Three-dimensional reconstructions of the immunostained astrocytes were performed and morphological classification was done based on hierarchical cluster and discriminant analysis of multimodal morphometric features. We found two morphological phenotypes of astrocytes in the newcomers which differentially increased its morphological complexities as wintering period progresses towards the pre-migration window. Taken together, our findings demonstrate that the long-distance non-stop flight and wintering period differentially affected the two astrocytes morphotypes, suggesting distinct physiological roles for these cells. We suggest that morphological changes during the wintering period, may be part of the adaptive plasticity of the local hippocampal circuits of A. interpres in preparation for the long journey back to their breeding sites in the north hemisphere. |
publishDate |
2020 |
dc.date.accessioned.fl_str_mv |
2020-06-30T14:13:15Z |
dc.date.available.fl_str_mv |
2020-06-30T14:13:15Z |
dc.date.issued.fl_str_mv |
2020 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
COSTA, Emanuel Ramos da et al. Changes in hippocampal astrocyte morphology of Ruddy turnstone (Arenaria interpres) during the wintering period at the mangroves of Amazon. Journal of Chemical Neuroanatomy, v. 108, n. xx, 101805, Oct. 2020. DOI: https://doi.org/10.1016/j.jchemneu.2020.101805. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/4105 |
dc.identifier.issn.-.fl_str_mv |
1873-6300 |
dc.identifier.doi.pt_BR.fl_str_mv |
10.1016/j.jchemneu.2020.101805 |
identifier_str_mv |
COSTA, Emanuel Ramos da et al. Changes in hippocampal astrocyte morphology of Ruddy turnstone (Arenaria interpres) during the wintering period at the mangroves of Amazon. Journal of Chemical Neuroanatomy, v. 108, n. xx, 101805, Oct. 2020. DOI: https://doi.org/10.1016/j.jchemneu.2020.101805. 1873-6300 10.1016/j.jchemneu.2020.101805 |
url |
https://patua.iec.gov.br/handle/iec/4105 |
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eng |
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eng |
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Elsevier |
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Elsevier |
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