Dengue virus serotype 2 genotype III evolution during the 2019 outbreak in Mato Grosso, Midwestern Brazil
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/6924 |
Resumo: | DENV-2 was the main responsible for a 70% increase in dengue incidence in Brazil during 2019. That year, our metagenomic study by Illumina NextSeq on serum samples from acute febrile patients (n = 92) with suspected arbovirus infection, sampled in 22 cities of the state of Mato Grosso (MT), in the middle west of Brazil, revealed eight complete genomes and two near-complete sequences of DENV-2 genotype III, one Human parvovirus B19 genotype I (5,391 nt) and one Coxsackievirus A6 lineage D (4,514 nt). These DENV-2 sequences share the aminoacidic identities of BR4 lineage on E protein domains I, II and III, and were included in a clade with sequences of the same lineage circulating in the southeast of Brazil in the same year. Nevertheless, 11/34 non-synonymous mutations are unique to three strains inthis study, distributed in the E (n = 6), NS3 (n = 2) and NS5 (n = 3) proteins. Other 14 aa changes on C (n = 1), E (n = 3), NS1 (n = 2), NS2A (n = 1) and NS5 (n = 7) were first reported in a genotype III lineage, having been already reported only in other DENV-2 genotypes. All 10 sequences have mutations in the NS5 protein (14 different aa changes). Nine E protein aa changes found in two sequences, six of which are unique, are in the ectodomain; where the E:M272T change is on the hinge of the E protein at domain II, in a region critical for the anchoring to the host cell receptor. The NS5:G81R mutation, in the methyltransferase domain, was found in one strain of this study. Altogether, these data points to an important evolution of DENV-2 genotype III lineage BR4 during this outbreak in 2019 in MT. Genomic surveillance is essential to detect virus etiology and evolution, possibly related to immune evasion and viral fitness changes leading to future novel outbreaks. |
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Santos, Marcelo Adriano Mendes dosPavon, Janeth Aracely RamirezDias, Lucas SilvaViniski, Ana ElisaSouza, Claudio Luis CamposOliveira, Elaine Cristina deAzevedo, Vergínia Correa deSilva, Sandro Patroca daCruz, Ana Cecília RibeiroMedeiros, Daniele Barbosa de AlmeidaNunes, Marcio Roberto TeixeiraSlhessarenko, Renata Dezengrini2023-09-04T17:22:05Z2023-09-04T17:22:05Z2023SANTOS, Marcelo Adriano Mendes dos et al. Dengue virus serotype 2 genotype III evolution during the 2019 outbreak in Mato Grosso, Midwestern Brazil. Infection, Genetics and Evolution, v. 113, n. 105487, Sept. 2023. DOI: https://doi.org/10.1016/j.meegid.2023.105487.1567-1348https://patua.iec.gov.br/handle/iec/692410.1016/j.meegid.2023.105487DENV-2 was the main responsible for a 70% increase in dengue incidence in Brazil during 2019. That year, our metagenomic study by Illumina NextSeq on serum samples from acute febrile patients (n = 92) with suspected arbovirus infection, sampled in 22 cities of the state of Mato Grosso (MT), in the middle west of Brazil, revealed eight complete genomes and two near-complete sequences of DENV-2 genotype III, one Human parvovirus B19 genotype I (5,391 nt) and one Coxsackievirus A6 lineage D (4,514 nt). These DENV-2 sequences share the aminoacidic identities of BR4 lineage on E protein domains I, II and III, and were included in a clade with sequences of the same lineage circulating in the southeast of Brazil in the same year. Nevertheless, 11/34 non-synonymous mutations are unique to three strains inthis study, distributed in the E (n = 6), NS3 (n = 2) and NS5 (n = 3) proteins. Other 14 aa changes on C (n = 1), E (n = 3), NS1 (n = 2), NS2A (n = 1) and NS5 (n = 7) were first reported in a genotype III lineage, having been already reported only in other DENV-2 genotypes. All 10 sequences have mutations in the NS5 protein (14 different aa changes). Nine E protein aa changes found in two sequences, six of which are unique, are in the ectodomain; where the E:M272T change is on the hinge of the E protein at domain II, in a region critical for the anchoring to the host cell receptor. The NS5:G81R mutation, in the methyltransferase domain, was found in one strain of this study. Altogether, these data points to an important evolution of DENV-2 genotype III lineage BR4 during this outbreak in 2019 in MT. Genomic surveillance is essential to detect virus etiology and evolution, possibly related to immune evasion and viral fitness changes leading to future novel outbreaks.DECIT/SCTIE/MS, CNPq, FAPEMATand SES-MT PPSUS 003/2017 grant (FAPEMAT.0290384/2018)Universidade Federal de Mato Grosso. Faculdade de Medicina. Programa de Pós-Graduação em Ciências da Saúde. Cuiaba, MT, Brazil / Universidade do Estado de Mato Grosso. Faculdade de Medicina. Caceres, MT, Brazil.Universidade Federal de Mato Grosso. Faculdade de Medicina. Programa de Pós-Graduação em Ciências da Saúde. Cuiaba, MT, Brazil.Universidade Federal de Mato Grosso. Faculdade de Medicina. Curso de Graduação em Medicina. Cuiaba, MT, Brazil.Secretaria de Estado da Saúde. Laboratório Central de Saúde Pública do Estado de Mato Grosso. Cuiaba, MT, Brazil.Secretaria de Estado da Saúde. Laboratório Central de Saúde Pública do Estado de Mato Grosso. Cuiaba, MT, Brazil.Secretaria de Estado da Saúde. Laboratório Central de Saúde Pública do Estado de Mato Grosso. Cuiaba, MT, Brazil.Secretaria de Estado da Saúde. Laboratório Central de Saúde Pública do Estado de Mato Grosso. Cuiaba, MT, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Universidade Federal de Mato Grosso. Faculdade de Medicina. Programa de Pós-Graduação em Ciências da Saúde. Cuiaba, MT, Brazil.engElsevierDengue virus serotype 2 genotype III evolution during the 2019 outbreak in Mato Grosso, Midwestern Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleDengue / classificaçãoDengue / genéticaVírus da Dengue / genéticaMetagenômicaMato Grosso (MT)Surtos de Doençasinfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALDengue virus serotype 2 genotype III evolution during the 2019 outbreak in Mato Grosso, Midwestern Brazil.pdfDengue virus serotype 2 genotype III evolution during the 2019 outbreak in Mato Grosso, Midwestern Brazil.pdfapplication/pdf5565349https://patua.iec.gov.br/bitstreams/6fa06d0c-f2bb-4739-9d7d-6383df2e7761/download40df049eb35899548b687cafc910e117MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82182https://patua.iec.gov.br/bitstreams/ec39aadf-e3db-46f8-bdcf-ceb843c38a54/download11832eea31b16df8613079d742d61793MD52TEXTDengue virus serotype 2 genotype III evolution during the 2019 outbreak in Mato Grosso, Midwestern Brazil.pdf.txtDengue virus serotype 2 genotype III evolution during the 2019 outbreak in Mato Grosso, Midwestern Brazil.pdf.txtExtracted texttext/plain61382https://patua.iec.gov.br/bitstreams/bd7a7156-2299-47e6-9d7b-52ffc4b2e6d5/download29ed0d1995f5df39a149132cdb985433MD53THUMBNAILDengue virus serotype 2 genotype III evolution during the 2019 outbreak in Mato Grosso, Midwestern Brazil.pdf.jpgDengue virus serotype 2 genotype III evolution during the 2019 outbreak in Mato Grosso, Midwestern Brazil.pdf.jpgGenerated Thumbnailimage/jpeg5714https://patua.iec.gov.br/bitstreams/938dff6c-9c1e-4aea-8277-b908e977f7a9/downloadfbdb0c453f24e5127ccbe3ed236b347fMD54iec/69242023-09-04 17:54:21.653oai:patua.iec.gov.br:iec/6924https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2023-09-04T17:54:21Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)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 |
dc.title.pt_BR.fl_str_mv |
Dengue virus serotype 2 genotype III evolution during the 2019 outbreak in Mato Grosso, Midwestern Brazil |
title |
Dengue virus serotype 2 genotype III evolution during the 2019 outbreak in Mato Grosso, Midwestern Brazil |
spellingShingle |
Dengue virus serotype 2 genotype III evolution during the 2019 outbreak in Mato Grosso, Midwestern Brazil Santos, Marcelo Adriano Mendes dos Dengue / classificação Dengue / genética Vírus da Dengue / genética Metagenômica Mato Grosso (MT) Surtos de Doenças |
title_short |
Dengue virus serotype 2 genotype III evolution during the 2019 outbreak in Mato Grosso, Midwestern Brazil |
title_full |
Dengue virus serotype 2 genotype III evolution during the 2019 outbreak in Mato Grosso, Midwestern Brazil |
title_fullStr |
Dengue virus serotype 2 genotype III evolution during the 2019 outbreak in Mato Grosso, Midwestern Brazil |
title_full_unstemmed |
Dengue virus serotype 2 genotype III evolution during the 2019 outbreak in Mato Grosso, Midwestern Brazil |
title_sort |
Dengue virus serotype 2 genotype III evolution during the 2019 outbreak in Mato Grosso, Midwestern Brazil |
author |
Santos, Marcelo Adriano Mendes dos |
author_facet |
Santos, Marcelo Adriano Mendes dos Pavon, Janeth Aracely Ramirez Dias, Lucas Silva Viniski, Ana Elisa Souza, Claudio Luis Campos Oliveira, Elaine Cristina de Azevedo, Vergínia Correa de Silva, Sandro Patroca da Cruz, Ana Cecília Ribeiro Medeiros, Daniele Barbosa de Almeida Nunes, Marcio Roberto Teixeira Slhessarenko, Renata Dezengrini |
author_role |
author |
author2 |
Pavon, Janeth Aracely Ramirez Dias, Lucas Silva Viniski, Ana Elisa Souza, Claudio Luis Campos Oliveira, Elaine Cristina de Azevedo, Vergínia Correa de Silva, Sandro Patroca da Cruz, Ana Cecília Ribeiro Medeiros, Daniele Barbosa de Almeida Nunes, Marcio Roberto Teixeira Slhessarenko, Renata Dezengrini |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Santos, Marcelo Adriano Mendes dos Pavon, Janeth Aracely Ramirez Dias, Lucas Silva Viniski, Ana Elisa Souza, Claudio Luis Campos Oliveira, Elaine Cristina de Azevedo, Vergínia Correa de Silva, Sandro Patroca da Cruz, Ana Cecília Ribeiro Medeiros, Daniele Barbosa de Almeida Nunes, Marcio Roberto Teixeira Slhessarenko, Renata Dezengrini |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Dengue / classificação Dengue / genética Vírus da Dengue / genética Metagenômica Mato Grosso (MT) |
topic |
Dengue / classificação Dengue / genética Vírus da Dengue / genética Metagenômica Mato Grosso (MT) Surtos de Doenças |
dc.subject.decsPrimary.none.fl_str_mv |
Surtos de Doenças |
description |
DENV-2 was the main responsible for a 70% increase in dengue incidence in Brazil during 2019. That year, our metagenomic study by Illumina NextSeq on serum samples from acute febrile patients (n = 92) with suspected arbovirus infection, sampled in 22 cities of the state of Mato Grosso (MT), in the middle west of Brazil, revealed eight complete genomes and two near-complete sequences of DENV-2 genotype III, one Human parvovirus B19 genotype I (5,391 nt) and one Coxsackievirus A6 lineage D (4,514 nt). These DENV-2 sequences share the aminoacidic identities of BR4 lineage on E protein domains I, II and III, and were included in a clade with sequences of the same lineage circulating in the southeast of Brazil in the same year. Nevertheless, 11/34 non-synonymous mutations are unique to three strains inthis study, distributed in the E (n = 6), NS3 (n = 2) and NS5 (n = 3) proteins. Other 14 aa changes on C (n = 1), E (n = 3), NS1 (n = 2), NS2A (n = 1) and NS5 (n = 7) were first reported in a genotype III lineage, having been already reported only in other DENV-2 genotypes. All 10 sequences have mutations in the NS5 protein (14 different aa changes). Nine E protein aa changes found in two sequences, six of which are unique, are in the ectodomain; where the E:M272T change is on the hinge of the E protein at domain II, in a region critical for the anchoring to the host cell receptor. The NS5:G81R mutation, in the methyltransferase domain, was found in one strain of this study. Altogether, these data points to an important evolution of DENV-2 genotype III lineage BR4 during this outbreak in 2019 in MT. Genomic surveillance is essential to detect virus etiology and evolution, possibly related to immune evasion and viral fitness changes leading to future novel outbreaks. |
publishDate |
2023 |
dc.date.accessioned.fl_str_mv |
2023-09-04T17:22:05Z |
dc.date.available.fl_str_mv |
2023-09-04T17:22:05Z |
dc.date.issued.fl_str_mv |
2023 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
SANTOS, Marcelo Adriano Mendes dos et al. Dengue virus serotype 2 genotype III evolution during the 2019 outbreak in Mato Grosso, Midwestern Brazil. Infection, Genetics and Evolution, v. 113, n. 105487, Sept. 2023. DOI: https://doi.org/10.1016/j.meegid.2023.105487. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/6924 |
dc.identifier.issn.-.fl_str_mv |
1567-1348 |
dc.identifier.doi.pt_BR.fl_str_mv |
10.1016/j.meegid.2023.105487 |
identifier_str_mv |
SANTOS, Marcelo Adriano Mendes dos et al. Dengue virus serotype 2 genotype III evolution during the 2019 outbreak in Mato Grosso, Midwestern Brazil. Infection, Genetics and Evolution, v. 113, n. 105487, Sept. 2023. DOI: https://doi.org/10.1016/j.meegid.2023.105487. 1567-1348 10.1016/j.meegid.2023.105487 |
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https://patua.iec.gov.br/handle/iec/6924 |
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Elsevier |
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Elsevier |
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