Genetic sequence characterization and naturally acquired immune response to Plasmodium vivax Rhoptry Neck Protein 2 (PvRON2)
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/3522 |
Resumo: | BACKGROUND: The genetic diversity of malaria antigens often results in allele variant-specific immunity, imposing a great challenge to vaccine development. Rhoptry Neck Protein 2 (PvRON2) is a blood-stage antigen that plays a key role during the erythrocyte invasion of Plasmodium vivax. This study investigates the genetic diversity of PvRON2 and the naturally acquired immune response to P. vivax isolates. RESULTS: Here, the genetic diversity of PvRON21828-2080 and the naturally acquired humoral immune response against PvRON21828-2080 in infected and non-infected individuals from a vivax malaria endemic area in Brazil was reported. The diversity analysis of PvRON21828-2080 revealed that the protein is conserved in isolates in Brazil and worldwide. A total of 18 (19%) patients had IgG antibodies to PvRON21828-2080. Additionally, the analysis of the antibody response in individuals who were not acutely infected with malaria, but had been infected with malaria in the past indicated that 32 patients (33%) exhibited an IgG immune response against PvRON2. CONCLUSIONS: PvRON2 was conserved among the studied isolates. The presence of naturally acquired antibodies to this protein in the absence of the disease suggests that PvRON2 induces a long-term antibody response. These results indicate that PvRON2 is a potential malaria vaccine candidate. |
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Bittencourt, Najara CLeite, Juliana ASilva, Ana Beatriz I. EPimenta, Tamirys SSilva Filho, João LuizCassiano, Gustavo CapattiLopes, Stefanie C. PSantos, João Conrado Khouri dosBourgard, CatarinaNakaya, Helder IVentura, Ana Maria Revorêdo da SilvaLacerda, Marcus Vinícius Guimarães deFerreira, Marcelo UrbanoMachado, Ricardo L. DAlbrecht, LetusaCosta, Fabio T. M2018-11-12T17:05:57Z2018-11-12T17:05:57Z2018BITTENCOURT, Najara C. et al. Genetic sequence characterization and naturally acquired immune response to Plasmodium vivax Rhoptry Neck Protein 2 (PvRON2). Malaria Journal, v. 17, n. 1, p. 401, Oct. 2018. DOI: https://doi.org/10.1186/s12936-018-2543-7. Disponível em: https://malariajournal.biomedcentral.com/articles/10.1186/s12936-018-2543-7.1475-2875https://patua.iec.gov.br/handle/iec/352210.1186/s12936-018-2543-7BACKGROUND: The genetic diversity of malaria antigens often results in allele variant-specific immunity, imposing a great challenge to vaccine development. Rhoptry Neck Protein 2 (PvRON2) is a blood-stage antigen that plays a key role during the erythrocyte invasion of Plasmodium vivax. This study investigates the genetic diversity of PvRON2 and the naturally acquired immune response to P. vivax isolates. RESULTS: Here, the genetic diversity of PvRON21828-2080 and the naturally acquired humoral immune response against PvRON21828-2080 in infected and non-infected individuals from a vivax malaria endemic area in Brazil was reported. The diversity analysis of PvRON21828-2080 revealed that the protein is conserved in isolates in Brazil and worldwide. A total of 18 (19%) patients had IgG antibodies to PvRON21828-2080. Additionally, the analysis of the antibody response in individuals who were not acutely infected with malaria, but had been infected with malaria in the past indicated that 32 patients (33%) exhibited an IgG immune response against PvRON2. CONCLUSIONS: PvRON2 was conserved among the studied isolates. The presence of naturally acquired antibodies to this protein in the absence of the disease suggests that PvRON2 induces a long-term antibody response. These results indicate that PvRON2 is a potential malaria vaccine candidate.This work was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Grant 2012/16525-2, and fellowships grants 2015/02808-0 (to NB), 2013/25807-4 (to JAL), 2016/12855-9 (to JLS-F), 2015/20774-6 (to GCC), 2013/20509-5 (to CB). Evandro Chagas core grant (to RLDM). MUF, MVGL and FTMC are Conselho Nacional do Desenvolvimento Científico e Tecnológico (CNPq) research fellows.University of Campinas-UNICAMP. Department of Genetics, Evolution, Microbiology and Immunology. Laboratory of Tropical Diseases Prof. Dr. Luiz Jacintho da Silva. Campinas, SP, Brazil.University of Campinas-UNICAMP. Department of Genetics, Evolution, Microbiology and Immunology. Laboratory of Tropical Diseases Prof. Dr. Luiz Jacintho da Silva. Campinas, SP, Brazil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Ensaios Clínicos e Imunogenética em Malária. Ananindeua, PA, Brasil.University of Campinas-UNICAMP. Department of Genetics, Evolution, Microbiology and Immunology. Laboratory of Tropical Diseases Prof. Dr. Luiz Jacintho da Silva. Campinas, SP, Brazil.University of Campinas-UNICAMP. Department of Genetics, Evolution, Microbiology and Immunology. Laboratory of Tropical Diseases Prof. Dr. Luiz Jacintho da Silva. Campinas, SP, Brazil.Fundação Oswaldo Cruz. Instituto Leônidas & Maria Deane. Manaus, AM, Brazil / Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brazil.University of Campinas-UNICAMP. Department of Genetics, Evolution, Microbiology and Immunology. Laboratory of Tropical Diseases Prof. Dr. Luiz Jacintho da Silva. Campinas, SP, Brazil.University of Campinas-UNICAMP. Department of Genetics, Evolution, Microbiology and Immunology. Laboratory of Tropical Diseases Prof. Dr. Luiz Jacintho da Silva. Campinas, SP, Brazil.University of São Paulo. School of Pharmaceutical Sciences. São Paulo, SP, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Ensaios Clínicos e Imunogenética em Malária. Ananindeua, PA, Brasil.Fundação Oswaldo Cruz. Instituto Leônidas & Maria Deane. Manaus, AM, Brazil / Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brazil.University of São Paulo. Institute of Biomedical Sciences. Department of Parasitology. São Paulo, SP, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Ensaios Clínicos e Imunogenética em Malária. Ananindeua, PA, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brazil.University of Campinas-UNICAMP. Department of Genetics, Evolution, Microbiology and Immunology. Laboratory of Tropical Diseases Prof. Dr. Luiz Jacintho da Silva. Campinas, SP, Brazil.engBMCGenetic sequence characterization and naturally acquired immune response to Plasmodium vivax Rhoptry Neck Protein 2 (PvRON2)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleMalária vivax / parasitologiaPlasmodium vivax / parasitologiaReações Antígeno-AnticorpoAntígenosVariação Genética / imunologiaVacinas / imunologiainfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALGenetic sequence characterization and naturally acquired immune response to Plasmodium vivax Rhoptry Neck Protein 2 (PvRON2).pdfGenetic sequence characterization and naturally acquired immune response to Plasmodium vivax Rhoptry Neck Protein 2 (PvRON2).pdfapplication/pdf2033030https://patua.iec.gov.br/bitstreams/4c36c08d-7521-4ebe-916e-89e16f26313e/downloadc7dacab25816a40a38ab7945ede57e8bMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-871https://patua.iec.gov.br/bitstreams/dcfc2697-da9f-45fc-bd06-5baaac4797c6/download52f1732ea66fbd1123abe39f5373b797MD52TEXTGenetic sequence characterization and naturally acquired immune response to Plasmodium vivax Rhoptry Neck Protein 2 (PvRON2).pdf.txtGenetic sequence characterization and naturally acquired immune response to Plasmodium vivax Rhoptry Neck Protein 2 (PvRON2).pdf.txtExtracted texttext/plain58839https://patua.iec.gov.br/bitstreams/c9ce4de9-206e-42ab-a8b8-17bb32e91322/downloadcbcd9956e1acdb0e77a8beb0f3cb1a91MD55THUMBNAILGenetic sequence characterization and naturally acquired immune response to Plasmodium vivax Rhoptry Neck Protein 2 (PvRON2).pdf.jpgGenetic sequence characterization and naturally acquired immune response to Plasmodium vivax Rhoptry Neck Protein 2 (PvRON2).pdf.jpgGenerated Thumbnailimage/jpeg6253https://patua.iec.gov.br/bitstreams/fd906c48-7d28-4442-8a36-9042db23fa77/download227878ae4637a9c390d9b8bb84ec765aMD56iec/35222022-10-20 22:24:15.273oai:patua.iec.gov.br:iec/3522https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-20T22:24:15Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)falseVG9kb3Mgb3MgZG9jdW1lbnRvcyBkZXNzYSBjb2xlw6fDo28gc2VndWVtIGEgTGljZW7Dp2EgQ3JlYXRpdmUgY29tbW9ucy4= |
dc.title.pt_BR.fl_str_mv |
Genetic sequence characterization and naturally acquired immune response to Plasmodium vivax Rhoptry Neck Protein 2 (PvRON2) |
title |
Genetic sequence characterization and naturally acquired immune response to Plasmodium vivax Rhoptry Neck Protein 2 (PvRON2) |
spellingShingle |
Genetic sequence characterization and naturally acquired immune response to Plasmodium vivax Rhoptry Neck Protein 2 (PvRON2) Bittencourt, Najara C Malária vivax / parasitologia Plasmodium vivax / parasitologia Reações Antígeno-Anticorpo Antígenos Variação Genética / imunologia Vacinas / imunologia |
title_short |
Genetic sequence characterization and naturally acquired immune response to Plasmodium vivax Rhoptry Neck Protein 2 (PvRON2) |
title_full |
Genetic sequence characterization and naturally acquired immune response to Plasmodium vivax Rhoptry Neck Protein 2 (PvRON2) |
title_fullStr |
Genetic sequence characterization and naturally acquired immune response to Plasmodium vivax Rhoptry Neck Protein 2 (PvRON2) |
title_full_unstemmed |
Genetic sequence characterization and naturally acquired immune response to Plasmodium vivax Rhoptry Neck Protein 2 (PvRON2) |
title_sort |
Genetic sequence characterization and naturally acquired immune response to Plasmodium vivax Rhoptry Neck Protein 2 (PvRON2) |
author |
Bittencourt, Najara C |
author_facet |
Bittencourt, Najara C Leite, Juliana A Silva, Ana Beatriz I. E Pimenta, Tamirys S Silva Filho, João Luiz Cassiano, Gustavo Capatti Lopes, Stefanie C. P Santos, João Conrado Khouri dos Bourgard, Catarina Nakaya, Helder I Ventura, Ana Maria Revorêdo da Silva Lacerda, Marcus Vinícius Guimarães de Ferreira, Marcelo Urbano Machado, Ricardo L. D Albrecht, Letusa Costa, Fabio T. M |
author_role |
author |
author2 |
Leite, Juliana A Silva, Ana Beatriz I. E Pimenta, Tamirys S Silva Filho, João Luiz Cassiano, Gustavo Capatti Lopes, Stefanie C. P Santos, João Conrado Khouri dos Bourgard, Catarina Nakaya, Helder I Ventura, Ana Maria Revorêdo da Silva Lacerda, Marcus Vinícius Guimarães de Ferreira, Marcelo Urbano Machado, Ricardo L. D Albrecht, Letusa Costa, Fabio T. M |
author2_role |
author author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Bittencourt, Najara C Leite, Juliana A Silva, Ana Beatriz I. E Pimenta, Tamirys S Silva Filho, João Luiz Cassiano, Gustavo Capatti Lopes, Stefanie C. P Santos, João Conrado Khouri dos Bourgard, Catarina Nakaya, Helder I Ventura, Ana Maria Revorêdo da Silva Lacerda, Marcus Vinícius Guimarães de Ferreira, Marcelo Urbano Machado, Ricardo L. D Albrecht, Letusa Costa, Fabio T. M |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Malária vivax / parasitologia Plasmodium vivax / parasitologia Reações Antígeno-Anticorpo Antígenos Variação Genética / imunologia Vacinas / imunologia |
topic |
Malária vivax / parasitologia Plasmodium vivax / parasitologia Reações Antígeno-Anticorpo Antígenos Variação Genética / imunologia Vacinas / imunologia |
description |
BACKGROUND: The genetic diversity of malaria antigens often results in allele variant-specific immunity, imposing a great challenge to vaccine development. Rhoptry Neck Protein 2 (PvRON2) is a blood-stage antigen that plays a key role during the erythrocyte invasion of Plasmodium vivax. This study investigates the genetic diversity of PvRON2 and the naturally acquired immune response to P. vivax isolates. RESULTS: Here, the genetic diversity of PvRON21828-2080 and the naturally acquired humoral immune response against PvRON21828-2080 in infected and non-infected individuals from a vivax malaria endemic area in Brazil was reported. The diversity analysis of PvRON21828-2080 revealed that the protein is conserved in isolates in Brazil and worldwide. A total of 18 (19%) patients had IgG antibodies to PvRON21828-2080. Additionally, the analysis of the antibody response in individuals who were not acutely infected with malaria, but had been infected with malaria in the past indicated that 32 patients (33%) exhibited an IgG immune response against PvRON2. CONCLUSIONS: PvRON2 was conserved among the studied isolates. The presence of naturally acquired antibodies to this protein in the absence of the disease suggests that PvRON2 induces a long-term antibody response. These results indicate that PvRON2 is a potential malaria vaccine candidate. |
publishDate |
2018 |
dc.date.accessioned.fl_str_mv |
2018-11-12T17:05:57Z |
dc.date.available.fl_str_mv |
2018-11-12T17:05:57Z |
dc.date.issued.fl_str_mv |
2018 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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dc.identifier.citation.fl_str_mv |
BITTENCOURT, Najara C. et al. Genetic sequence characterization and naturally acquired immune response to Plasmodium vivax Rhoptry Neck Protein 2 (PvRON2). Malaria Journal, v. 17, n. 1, p. 401, Oct. 2018. DOI: https://doi.org/10.1186/s12936-018-2543-7. Disponível em: https://malariajournal.biomedcentral.com/articles/10.1186/s12936-018-2543-7. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/3522 |
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1475-2875 |
dc.identifier.doi.pt_BR.fl_str_mv |
10.1186/s12936-018-2543-7 |
identifier_str_mv |
BITTENCOURT, Najara C. et al. Genetic sequence characterization and naturally acquired immune response to Plasmodium vivax Rhoptry Neck Protein 2 (PvRON2). Malaria Journal, v. 17, n. 1, p. 401, Oct. 2018. DOI: https://doi.org/10.1186/s12936-018-2543-7. Disponível em: https://malariajournal.biomedcentral.com/articles/10.1186/s12936-018-2543-7. 1475-2875 10.1186/s12936-018-2543-7 |
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