Identification of French Guiana sand flies using MALDI-TOF mass spectrometry with a new mass spectra library
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/3614 |
Resumo: | Phlebotomine sand flies are insects that are highly relevant in medicine, particularly as the sole proven vectors of leishmaniasis. Accurate identification of sand fly species is an essential prerequisite for eco-epidemiological studies aiming to better understand the disease. Traditional morphological identification is painstaking and time-consuming, and molecular methods for extensive screening remain expensive. Recent studies have shown that matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a promising tool for rapid and cost-effective identification of arthropod vectors, including sand flies. The aim of this study was to validate the use of MALDI-TOF MS for the identification of Northern Amazonian sand flies. We constituted a MALDI-TOF MS reference database comprising 29 species of sand flies that were field-collected in French Guiana, which are expected to cover many of the more common species of the Northern Amazonian region, including known vectors of leishmaniasis. Carrying out a blind test, all the sand flies tested (n = 157) with a log (score) threshold greater than 1.7 were correctly identified at the species level. We confirmed that MALDI-TOF MS protein profiling is a useful tool for the study of sand flies, including neotropical species, known for their great diversity. An application that includes the spectra generated here will be available to the scientific community in the near future via an online platform. |
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Chavy, AgatheNabet, CécileNormand, Anne CécileKocher, ArthurGinouves, MarinePrévot, GhislaineSantos, Thiago Vasconcelos dosDemar, MagaliePiarroux, RenaudThoisy, Benoît de2019-02-26T12:42:18Z2019-02-26T12:42:18Z2019CHAVY, Agathe et al. Identification of French Guiana sand flies using MALDI-TOF mass spectrometry with a new mass spectra library. PLoS Neglected Tropical Diseases, v. 13, n. 2, e0007031, p. 1-19, Feb. 2019.1935-2727https://patua.iec.gov.br/handle/iec/361410.1371/journal.pntd.0007031Phlebotomine sand flies are insects that are highly relevant in medicine, particularly as the sole proven vectors of leishmaniasis. Accurate identification of sand fly species is an essential prerequisite for eco-epidemiological studies aiming to better understand the disease. Traditional morphological identification is painstaking and time-consuming, and molecular methods for extensive screening remain expensive. Recent studies have shown that matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a promising tool for rapid and cost-effective identification of arthropod vectors, including sand flies. The aim of this study was to validate the use of MALDI-TOF MS for the identification of Northern Amazonian sand flies. We constituted a MALDI-TOF MS reference database comprising 29 species of sand flies that were field-collected in French Guiana, which are expected to cover many of the more common species of the Northern Amazonian region, including known vectors of leishmaniasis. Carrying out a blind test, all the sand flies tested (n = 157) with a log (score) threshold greater than 1.7 were correctly identified at the species level. We confirmed that MALDI-TOF MS protein profiling is a useful tool for the study of sand flies, including neotropical species, known for their great diversity. An application that includes the spectra generated here will be available to the scientific community in the near future via an online platform.This study was conducted within the RESERVOIRS program supported by European (ERDF/FEDER) funds and assistance from Collectivité Territoriale de la Guyane and Direction Régionale pour la Recherche et la Technologie, and the MicroBIOME project granted by Laboratoire d’Excellence CEBA “Investissement d’Avenir” and managed by the Agence Nationale de la Recherche (CEBA, Ref. ANR-10-LABEX-25-01). AC benefits from a PhD grant from the Université de Guyane. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Institut Pasteur de la Guyane. Laboratoire des Interactions Virus-Hôtes. Cayenne, French Guiana / Université de Guyane. Medicine Department. Laboratoire des Ecosystèmes Amazoniens et Pathologie Tropicale. Cayenne, French Guiana.Sorbonne Université. INSERM, Institut Pierre-Louis d’Epidemiologie et de Santé´ Publique. AP-HP, Groupe Hospitalier Pitié´-Salpêtrière. Service de Parasitologie-Mycologie. Paris, France.Sorbonne Université. INSERM, Institut Pierre-Louis d’Epidemiologie et de Santé´ Publique. AP-HP, Groupe Hospitalier Pitié´-Salpêtrière. Service de Parasitologie-Mycologie. Paris, France.Universite´ Toulouse III Paul Sabatier. CNRS. ENFA. UMR5174 EDB (Laboratoire Evolution et Diversite´ Biologique). Toulouse, France.Université de Guyane. Medicine Department. Laboratoire des Ecosystèmes Amazoniens et Pathologie Tropicale. Cayenne, French Guiana.Université de Guyane. Medicine Department. Laboratoire des Ecosystèmes Amazoniens et Pathologie Tropicale. Cayenne, French Guiana.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Universitaire de Parasitologie-Mycologie. Centre Hospitalier Andrée Rosemon. Laboratoire Hospitalo. Laboratoire Associe´ du CNR Leishmaniose. Cayenne, French Guiana.Sorbonne Université. INSERM, Institut Pierre-Louis d’Epidemiologie et de Santé´ Publique. AP-HP, Groupe Hospitalier Pitié´-Salpêtrière. Service de Parasitologie-Mycologie. Paris, France.Institut Pasteur de la Guyane. Laboratoire des Interactions Virus-Hôtes. Cayenne, French Guiana.engPublic Library of ScienceIdentification of French Guiana sand flies using MALDI-TOF mass spectrometry with a new mass spectra libraryinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePsychodidae / parasitologiaLeishmaniose / transmissãoPhlebotomus / parasitologiaVetores de DoençasEspectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz / métodosGuiana Francesa (GF)Região Amazônica (BR)info:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALIdentification of French Guiana sand flies using MALDI-TOF mass spectrometry with a new mass spectra library.pdfIdentification of French Guiana sand flies using MALDI-TOF mass spectrometry with a new mass spectra library.pdfapplication/pdf2645733https://patua.iec.gov.br/bitstreams/8b21dd84-4250-44f1-87a7-5fd1f290f26f/download019e725296eb5f687fb8796cdf3532e9MD51LICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv |
Identification of French Guiana sand flies using MALDI-TOF mass spectrometry with a new mass spectra library |
title |
Identification of French Guiana sand flies using MALDI-TOF mass spectrometry with a new mass spectra library |
spellingShingle |
Identification of French Guiana sand flies using MALDI-TOF mass spectrometry with a new mass spectra library Chavy, Agathe Psychodidae / parasitologia Leishmaniose / transmissão Phlebotomus / parasitologia Vetores de Doenças Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz / métodos Guiana Francesa (GF) Região Amazônica (BR) |
title_short |
Identification of French Guiana sand flies using MALDI-TOF mass spectrometry with a new mass spectra library |
title_full |
Identification of French Guiana sand flies using MALDI-TOF mass spectrometry with a new mass spectra library |
title_fullStr |
Identification of French Guiana sand flies using MALDI-TOF mass spectrometry with a new mass spectra library |
title_full_unstemmed |
Identification of French Guiana sand flies using MALDI-TOF mass spectrometry with a new mass spectra library |
title_sort |
Identification of French Guiana sand flies using MALDI-TOF mass spectrometry with a new mass spectra library |
author |
Chavy, Agathe |
author_facet |
Chavy, Agathe Nabet, Cécile Normand, Anne Cécile Kocher, Arthur Ginouves, Marine Prévot, Ghislaine Santos, Thiago Vasconcelos dos Demar, Magalie Piarroux, Renaud Thoisy, Benoît de |
author_role |
author |
author2 |
Nabet, Cécile Normand, Anne Cécile Kocher, Arthur Ginouves, Marine Prévot, Ghislaine Santos, Thiago Vasconcelos dos Demar, Magalie Piarroux, Renaud Thoisy, Benoît de |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Chavy, Agathe Nabet, Cécile Normand, Anne Cécile Kocher, Arthur Ginouves, Marine Prévot, Ghislaine Santos, Thiago Vasconcelos dos Demar, Magalie Piarroux, Renaud Thoisy, Benoît de |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Psychodidae / parasitologia Leishmaniose / transmissão Phlebotomus / parasitologia Vetores de Doenças Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz / métodos Guiana Francesa (GF) Região Amazônica (BR) |
topic |
Psychodidae / parasitologia Leishmaniose / transmissão Phlebotomus / parasitologia Vetores de Doenças Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz / métodos Guiana Francesa (GF) Região Amazônica (BR) |
description |
Phlebotomine sand flies are insects that are highly relevant in medicine, particularly as the sole proven vectors of leishmaniasis. Accurate identification of sand fly species is an essential prerequisite for eco-epidemiological studies aiming to better understand the disease. Traditional morphological identification is painstaking and time-consuming, and molecular methods for extensive screening remain expensive. Recent studies have shown that matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a promising tool for rapid and cost-effective identification of arthropod vectors, including sand flies. The aim of this study was to validate the use of MALDI-TOF MS for the identification of Northern Amazonian sand flies. We constituted a MALDI-TOF MS reference database comprising 29 species of sand flies that were field-collected in French Guiana, which are expected to cover many of the more common species of the Northern Amazonian region, including known vectors of leishmaniasis. Carrying out a blind test, all the sand flies tested (n = 157) with a log (score) threshold greater than 1.7 were correctly identified at the species level. We confirmed that MALDI-TOF MS protein profiling is a useful tool for the study of sand flies, including neotropical species, known for their great diversity. An application that includes the spectra generated here will be available to the scientific community in the near future via an online platform. |
publishDate |
2019 |
dc.date.accessioned.fl_str_mv |
2019-02-26T12:42:18Z |
dc.date.available.fl_str_mv |
2019-02-26T12:42:18Z |
dc.date.issued.fl_str_mv |
2019 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
CHAVY, Agathe et al. Identification of French Guiana sand flies using MALDI-TOF mass spectrometry with a new mass spectra library. PLoS Neglected Tropical Diseases, v. 13, n. 2, e0007031, p. 1-19, Feb. 2019. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/3614 |
dc.identifier.issn.-.fl_str_mv |
1935-2727 |
dc.identifier.doi.pt_BR.fl_str_mv |
10.1371/journal.pntd.0007031 |
identifier_str_mv |
CHAVY, Agathe et al. Identification of French Guiana sand flies using MALDI-TOF mass spectrometry with a new mass spectra library. PLoS Neglected Tropical Diseases, v. 13, n. 2, e0007031, p. 1-19, Feb. 2019. 1935-2727 10.1371/journal.pntd.0007031 |
url |
https://patua.iec.gov.br/handle/iec/3614 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Public Library of Science |
publisher.none.fl_str_mv |
Public Library of Science |
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IEC |
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IEC |
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Repositório Digital do Instituto Evandro Chagas (Patuá) |
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Repositório Digital do Instituto Evandro Chagas (Patuá) |
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