Susceptibility of cebus apella Monkey (Primates: Cebidae) to experimental Leishmania (L) infantum chagasi-infection

Detalhes bibliográficos
Autor(a) principal: Carneiro, Liliane Almeida
Data de Publicação: 2011
Outros Autores: Silveira, Fernando Tobias, Campos, Marliane Batista, Brígido, Maria do Carmo de Oliveira, Gomes, Claudia Maria de Castro, Corbett, Carlos Eduardo Pereira, Laurenti, Márcia Dalastra
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
Texto Completo: https://patua.iec.gov.br/handle/iec/697
Resumo: In Amazonian Brazil, the Cebus apella monkey (Primates: Cebidae) has been associated with the enzootic cycle of Leishmania (V.) shawi, a dermotropic parasite causing American cutaneous leishmaniasis (ACL). It has also been successfully used as animal model for studying cutaneous leishmaniasis. In this work, there has been investigated its susceptibility to experimental Leishmania (L.) infantum chagasi-infection, the etiologic agent of American visceral leishmaniasis (AVL). There were used ten C. apella specimens, eight adult and two young, four males and six females, all born and raised in captivity. Two experimental infection protocols were performed: i) six monkeys were inoculated, intra-dermal via (ID), into the base of the tail with 2 x 106 promastigotes forms from the stationary phase culture medium; ii) other four monkeys were inoculated with 3 x 107 amastigotes forms from the visceral infection of infected hamsters by two different via: a) two by intravenous via (IV) and, b) other two by intra-peritoneal via (IP). The parameters of infection evaluation included: a) clinical: physical exam of abdomen, weigh and body temperature; b) parasitological: needle aspiration of the bone-marrow for searching of amastigotes (Giemsa-stained smears) and promastigotes forms (culture medium); c) immunological: Indirect fluorescence antibody test (IFAT) and, Delayed-type hypersensitivity (DTH). In the six monkeys ID inoculated (promastigotes forms) all parameters of infection evaluation were negative during the 12 months period of follow-up. Among the four monkeys inoculated with amastigotes forms, two IV inoculated showed the parasite in the bone-marrow from the first toward to the sixth month p.i. and following that they cleared the infection, whereas the other two IP inoculated were totally negative. These four monkeys showed specific IgG-antibody response since the third month p.i. (IP: 1/80 and IV: 1/320 IgG) toward to the 12th month (IP: 1/160 and IV: 1/5120). The DTH-conversion occurred in only one IV inoculated monkey with a strong (30 mm) skin reaction. Considering these results, we do not encourage the use of C. apella monkey as animal model for studying the AVL.
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spelling Carneiro, Liliane AlmeidaSilveira, Fernando TobiasCampos, Marliane BatistaBrígido, Maria do Carmo de OliveiraGomes, Claudia Maria de CastroCorbett, Carlos Eduardo PereiraLaurenti, Márcia Dalastra2016-01-26T11:36:41Z2016-01-26T11:36:41Z2011CARNEIRO, Liliane Almeida et al. Susceptibility of cebus apella Monkey (Primates: Cebidae) to experimental Leishmania (L) infantum chagasi-infection. Revista do Instituto de Medicina Tropical de São Paulo, v. 53, n. 1, p. 45-50, Jan.-Feb. 20110036-4665https://patua.iec.gov.br/handle/iec/69710.1590/S0036-46652011000100008In Amazonian Brazil, the Cebus apella monkey (Primates: Cebidae) has been associated with the enzootic cycle of Leishmania (V.) shawi, a dermotropic parasite causing American cutaneous leishmaniasis (ACL). It has also been successfully used as animal model for studying cutaneous leishmaniasis. In this work, there has been investigated its susceptibility to experimental Leishmania (L.) infantum chagasi-infection, the etiologic agent of American visceral leishmaniasis (AVL). There were used ten C. apella specimens, eight adult and two young, four males and six females, all born and raised in captivity. Two experimental infection protocols were performed: i) six monkeys were inoculated, intra-dermal via (ID), into the base of the tail with 2 x 106 promastigotes forms from the stationary phase culture medium; ii) other four monkeys were inoculated with 3 x 107 amastigotes forms from the visceral infection of infected hamsters by two different via: a) two by intravenous via (IV) and, b) other two by intra-peritoneal via (IP). The parameters of infection evaluation included: a) clinical: physical exam of abdomen, weigh and body temperature; b) parasitological: needle aspiration of the bone-marrow for searching of amastigotes (Giemsa-stained smears) and promastigotes forms (culture medium); c) immunological: Indirect fluorescence antibody test (IFAT) and, Delayed-type hypersensitivity (DTH). In the six monkeys ID inoculated (promastigotes forms) all parameters of infection evaluation were negative during the 12 months period of follow-up. Among the four monkeys inoculated with amastigotes forms, two IV inoculated showed the parasite in the bone-marrow from the first toward to the sixth month p.i. and following that they cleared the infection, whereas the other two IP inoculated were totally negative. These four monkeys showed specific IgG-antibody response since the third month p.i. (IP: 1/80 and IV: 1/320 IgG) toward to the 12th month (IP: 1/160 and IV: 1/5120). The DTH-conversion occurred in only one IV inoculated monkey with a strong (30 mm) skin reaction. Considering these results, we do not encourage the use of C. apella monkey as animal model for studying the AVL.Na Amazônia Brasileira, o macaco Cebus apella (Primata: Cebidae) tem sido associado com o ciclo enzoótico da Leishmania (V.) shawi, um parasito dermotrópico causador da Leishmaniose Tegumentar Americana (LTA). Ele tem sido também empregado com sucesso como modelo experimental para estudo da leishmaniose tegumentar. Neste trabalho, foi investigada sua susceptibilidade à infecção experimental por Leishmania (L.) infantum chagasi, o agente etiológico da Leishmaniose Visceral Americana (LVA). Foram usados dez espécimes de C. apella oito adultos e dois jovens, quatro machos e seis fêmeas, todos nascidos e criados em cativeiro. Dois protocolos de infecção experimental foram feitos: i) seis macacos foram inoculados por via intradérmica (ID), na base da cauda com 2x106 formas promastigotas em fase estacionária de crescimento; ii) outros quatro macacos foram inoculados com 3x107 formas amastigotas de infecção visceral de hamsteres por duas vias diferentes: a) dois por via intravenosa (IV) e, b) outros dois pela via intraperitoneal (IP). A avaliação da infecção incluiu parâmetros: clínico: exame físico do abdômen, peso e temperatura corporal; b) parasitológico: aspirado de medula óssea por agulha para procura de amastigotas (esfregaço corado por Giemsa) e formas promastigotas (meio de cultura); c) imunológico: Reação de Imunofluorescência Indireta (RIFI) e, resposta de hipersensibilidade tardia (DTH). Nos seis macacos inoculados ID (formas promastigotas) todos os parâmetros de avaliação da infecção foram negativos durante o período de 12 meses. Entre os quatro macacos inoculados com formas amastigotas, dois inoculados IV mostraram parasitos na medula óssea do primeiro ao sexto mês p.i. e em seguida houve a resolução da infecção, no entanto os outros dois inoculados IP foram totalmente negativos. Esses quatro macacos apresentaram resposta específica de anticorpo IgG desde o terceiro mês p.i. (IP: 1/80 e IV: 1/320) até o décimo segundo mês (IP: 1/160 e IV: 1/5120). A conversão DTH ocorreu em apenas um macaco inoculado IV com uma forte reação na pele (30 mm). Considerando esses resultados, nós não recomendamos o uso do macaco C. apella como modelo animal para estudo da LVA.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Universidade Federal do Pará. Núcleo de Medicina Tropical. Belém, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Universidade de São Paulo. Faculdade de Medicina. Departamento de Patologia. São Paulo, SP, Brasil.Universidade de São Paulo. Faculdade de Medicina. Departamento de Patologia. São Paulo, SP, Brasil.Universidade de São Paulo. Faculdade de Medicina. Departamento de Patologia. 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dc.title.pt_BR.fl_str_mv Susceptibility of cebus apella Monkey (Primates: Cebidae) to experimental Leishmania (L) infantum chagasi-infection
dc.title.alternative.pt_BR.fl_str_mv Susceptibilidade do Macaco cebus apella (Primata: Cebidae) à infecção experimental por Leishmania (L) infantum chagasi
title Susceptibility of cebus apella Monkey (Primates: Cebidae) to experimental Leishmania (L) infantum chagasi-infection
spellingShingle Susceptibility of cebus apella Monkey (Primates: Cebidae) to experimental Leishmania (L) infantum chagasi-infection
Carneiro, Liliane Almeida
Primatas / parasitologia
Cebidae
Cebus / classificação
Leishmania infantum / parasitologia
title_short Susceptibility of cebus apella Monkey (Primates: Cebidae) to experimental Leishmania (L) infantum chagasi-infection
title_full Susceptibility of cebus apella Monkey (Primates: Cebidae) to experimental Leishmania (L) infantum chagasi-infection
title_fullStr Susceptibility of cebus apella Monkey (Primates: Cebidae) to experimental Leishmania (L) infantum chagasi-infection
title_full_unstemmed Susceptibility of cebus apella Monkey (Primates: Cebidae) to experimental Leishmania (L) infantum chagasi-infection
title_sort Susceptibility of cebus apella Monkey (Primates: Cebidae) to experimental Leishmania (L) infantum chagasi-infection
author Carneiro, Liliane Almeida
author_facet Carneiro, Liliane Almeida
Silveira, Fernando Tobias
Campos, Marliane Batista
Brígido, Maria do Carmo de Oliveira
Gomes, Claudia Maria de Castro
Corbett, Carlos Eduardo Pereira
Laurenti, Márcia Dalastra
author_role author
author2 Silveira, Fernando Tobias
Campos, Marliane Batista
Brígido, Maria do Carmo de Oliveira
Gomes, Claudia Maria de Castro
Corbett, Carlos Eduardo Pereira
Laurenti, Márcia Dalastra
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Carneiro, Liliane Almeida
Silveira, Fernando Tobias
Campos, Marliane Batista
Brígido, Maria do Carmo de Oliveira
Gomes, Claudia Maria de Castro
Corbett, Carlos Eduardo Pereira
Laurenti, Márcia Dalastra
dc.subject.decsPrimary.pt_BR.fl_str_mv Primatas / parasitologia
Cebidae
Cebus / classificação
Leishmania infantum / parasitologia
topic Primatas / parasitologia
Cebidae
Cebus / classificação
Leishmania infantum / parasitologia
description In Amazonian Brazil, the Cebus apella monkey (Primates: Cebidae) has been associated with the enzootic cycle of Leishmania (V.) shawi, a dermotropic parasite causing American cutaneous leishmaniasis (ACL). It has also been successfully used as animal model for studying cutaneous leishmaniasis. In this work, there has been investigated its susceptibility to experimental Leishmania (L.) infantum chagasi-infection, the etiologic agent of American visceral leishmaniasis (AVL). There were used ten C. apella specimens, eight adult and two young, four males and six females, all born and raised in captivity. Two experimental infection protocols were performed: i) six monkeys were inoculated, intra-dermal via (ID), into the base of the tail with 2 x 106 promastigotes forms from the stationary phase culture medium; ii) other four monkeys were inoculated with 3 x 107 amastigotes forms from the visceral infection of infected hamsters by two different via: a) two by intravenous via (IV) and, b) other two by intra-peritoneal via (IP). The parameters of infection evaluation included: a) clinical: physical exam of abdomen, weigh and body temperature; b) parasitological: needle aspiration of the bone-marrow for searching of amastigotes (Giemsa-stained smears) and promastigotes forms (culture medium); c) immunological: Indirect fluorescence antibody test (IFAT) and, Delayed-type hypersensitivity (DTH). In the six monkeys ID inoculated (promastigotes forms) all parameters of infection evaluation were negative during the 12 months period of follow-up. Among the four monkeys inoculated with amastigotes forms, two IV inoculated showed the parasite in the bone-marrow from the first toward to the sixth month p.i. and following that they cleared the infection, whereas the other two IP inoculated were totally negative. These four monkeys showed specific IgG-antibody response since the third month p.i. (IP: 1/80 and IV: 1/320 IgG) toward to the 12th month (IP: 1/160 and IV: 1/5120). The DTH-conversion occurred in only one IV inoculated monkey with a strong (30 mm) skin reaction. Considering these results, we do not encourage the use of C. apella monkey as animal model for studying the AVL.
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2016-01-26T11:36:41Z
dc.date.available.fl_str_mv 2016-01-26T11:36:41Z
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dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv CARNEIRO, Liliane Almeida et al. Susceptibility of cebus apella Monkey (Primates: Cebidae) to experimental Leishmania (L) infantum chagasi-infection. Revista do Instituto de Medicina Tropical de São Paulo, v. 53, n. 1, p. 45-50, Jan.-Feb. 2011
dc.identifier.uri.fl_str_mv https://patua.iec.gov.br/handle/iec/697
dc.identifier.issn.-.fl_str_mv 0036-4665
dc.identifier.doi.-.fl_str_mv 10.1590/S0036-46652011000100008
identifier_str_mv CARNEIRO, Liliane Almeida et al. Susceptibility of cebus apella Monkey (Primates: Cebidae) to experimental Leishmania (L) infantum chagasi-infection. Revista do Instituto de Medicina Tropical de São Paulo, v. 53, n. 1, p. 45-50, Jan.-Feb. 2011
0036-4665
10.1590/S0036-46652011000100008
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