Polymorphisms in Plasmodium vivax circumsporozoite protein (CSP) influence parasite burden and cytokine balance in a pre-amazon endemic area from Brazil
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/2430 |
Resumo: | Mechanisms involved in severe P. vivax malaria remain unclear. Parasite polymorphisms, parasite load and host cytokine profile may influence the course of infection. In this study, we investigated the influence of circumsporozoite protein (CSP) polymorphisms on parasite load and cytokine profile in patients with vivax malaria. A cross-sectional study was carried out in three cities: São Luís, Cedral and Buriticupu, Maranhão state, Brazil, areas of high prevalence of P. vivax. Interleukin (IL)-2, IL-4, IL-10, IL-6, IL-17, tumor necrosis factor alpha (TNF-α, interferon gamma (IFN-γ and transforming growth factor beta (TGF-β were quantified in blood plasma of patients and in supernatants from peripheral blood mononuclear cell (PBMC) cultures. Furthermore, the levels of cytokines and parasite load were correlated with VK210, VK247 and P. vivax-like CSP variants. Patients infected with P. vivax showed increased IL-10 and IL-6 levels, which correlated with the parasite load, however, in multiple comparisons, only IL-10 kept this association. A regulatory cytokine profile prevailed in plasma, while an inflammatory profile prevailed in PBMC culture supernatants and these patterns were related to CSP polymorphisms. VK247 infected patients showed higher parasitaemia and IL-6 concentrations, which were not associated to IL-10 anti-inflammatory effect. By contrast, in VK210 patients, these two cytokines showed a strong positive correlation and the parasite load was lower. Patients with the VK210 variant showed a regulatory cytokine profile in plasma, while those infected with the VK247 variant have a predominantly inflammatory cytokine profile and higher parasite loads, which altogether may result in more complications in infection. In conclusion, we propose that CSP polymorphisms is associated to the increase of non-regulated inflammatory immune responses, which in turn may be associated with the outcome of infection. |
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Ribeiro, Bruno de PauloCassiano, Gustavo CapattiSouza, Rodrigo Medeiros deCysne, Dalila NunesGrisotto, Marcos Augusto GrigolinSantos, Ana Paula Silva de Azevedo dosMarinho, Cláudio Romero FariasMachado, Ricardo Luiz DantasNascimento, Flávia Raquel Fernandes2017-03-24T11:54:20Z2017-03-24T11:54:20Z2016RIBEIRO, Bruno de Paulo et al. Polymorphisms in Plasmodium vivax circumsporozoite protein (CSP) influence parasite burden and cytokine balance in a pre-amazon endemic area from Brazil. PLoS Neglected Tropical Diseases, v. 10, n. 3, p. 1-9, 2016.1935-2735https://patua.iec.gov.br/handle/iec/243010.1371/journal.pntd.0004479Mechanisms involved in severe P. vivax malaria remain unclear. Parasite polymorphisms, parasite load and host cytokine profile may influence the course of infection. In this study, we investigated the influence of circumsporozoite protein (CSP) polymorphisms on parasite load and cytokine profile in patients with vivax malaria. A cross-sectional study was carried out in three cities: São Luís, Cedral and Buriticupu, Maranhão state, Brazil, areas of high prevalence of P. vivax. Interleukin (IL)-2, IL-4, IL-10, IL-6, IL-17, tumor necrosis factor alpha (TNF-α, interferon gamma (IFN-γ and transforming growth factor beta (TGF-β were quantified in blood plasma of patients and in supernatants from peripheral blood mononuclear cell (PBMC) cultures. Furthermore, the levels of cytokines and parasite load were correlated with VK210, VK247 and P. vivax-like CSP variants. Patients infected with P. vivax showed increased IL-10 and IL-6 levels, which correlated with the parasite load, however, in multiple comparisons, only IL-10 kept this association. A regulatory cytokine profile prevailed in plasma, while an inflammatory profile prevailed in PBMC culture supernatants and these patterns were related to CSP polymorphisms. VK247 infected patients showed higher parasitaemia and IL-6 concentrations, which were not associated to IL-10 anti-inflammatory effect. By contrast, in VK210 patients, these two cytokines showed a strong positive correlation and the parasite load was lower. Patients with the VK210 variant showed a regulatory cytokine profile in plasma, while those infected with the VK247 variant have a predominantly inflammatory cytokine profile and higher parasite loads, which altogether may result in more complications in infection. In conclusion, we propose that CSP polymorphisms is associated to the increase of non-regulated inflammatory immune responses, which in turn may be associated with the outcome of infection.Universidade Federal do Maranhão. Programa de Pós- graduação em Ciências da Saúde. São Luís, MA, Brazil / Universidade Federal do Maranhão. Laboratório de Imunofisiologia. São Luís, MA, Brazil.Faculdade de Medicina de São José do Rio Preto. Centro de Investigação de Microrganismos. São José do Rio Preto, SP, Brazil.Universidade de São Paulo. Instituto de Ciências Biológicas. Departamento de Parasitologia. São Paulo, SP, Brazil / Universidade Federal do Acre - Campus Floresta. Centro Multidisciplinar. Cruzeiro do Sul, AC, Brazil.Universidade Federal do Maranhão. Laboratório de Imunofisiologia. São Luís, MA, Brazil.Universidade CEUMA. Laboratório de Imunologia das Parasitoses. São Luís, MA, Brazil.Universidade Federal do Maranhão. Laboratório de Imunofisiologia. São Luís, MA, Brazil.Universidade de São Paulo. Instituto de Ciências Biológicas. Departamento de Parasitologia. São Paulo, SP, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Pesquisa Básica em Malária. Belém, PA, Brasil.Universidade Federal do Maranhão. Laboratório de Imunofisiologia. São Luís, MA, Brazil.application/pdfengPublic Library of SciencePolymorphisms in Plasmodium vivax circumsporozoite protein (CSP) influence parasite burden and cytokine balance in a pre-amazon endemic area from Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleMalária Vivax / parasitologiaPlasmodium vivax / imunologiaPlasmodium vivax / genéticaPolimorfismo GenéticoPolimorfismo de Fragmento de RestriçãoVariação GenéticaGenótipoCitocinasinfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALPolymorphisms in Plasmodium vivax circumsporozoite protein (CSP) influence parasite burden and cytokine balance in a pre-amazon endemic area from Brazil.pdfapplication/pdf605637https://patua.iec.gov.br/bitstreams/7a19362e-24c5-42f1-ae97-d1d9d7a05b5b/download3186f9d300d5b489fc4b94b87fb32e84MD51TEXTPolymorphisms in Plasmodium vivax Circumsporozoite Protein (CSP) influence Parasite Burden and Cytokine Balance in a Pre-Amazon endemic area from Brazil.pdf.txtExtracted texttext/plain47652https://patua.iec.gov.br/bitstreams/5dd3e237-59bc-4a33-9202-aebd5a51c301/downloada1430e64ac0d97d8afe651d6abb7d509MD52Polymorphisms in Plasmodium vivax circumsporozoite protein (CSP) influence parasite burden and cytokine balance in a pre-amazon endemic area from Brazil.pdf.txtPolymorphisms in Plasmodium vivax circumsporozoite protein (CSP) influence parasite burden and cytokine balance in a pre-amazon endemic area from Brazil.pdf.txtExtracted texttext/plain64784https://patua.iec.gov.br/bitstreams/8ed16d68-d762-498f-8b3b-f0e4179dcd4b/download45d65ec86dcb6f8ae59a17479a4f9226MD57THUMBNAILPolymorphisms in Plasmodium vivax Circumsporozoite Protein (CSP) influence Parasite Burden and Cytokine Balance in a Pre-Amazon endemic area from Brazil.pdf.jpgIM Thumbnailimage/jpeg4661https://patua.iec.gov.br/bitstreams/e9d5f4a9-23ef-44c3-8e2d-7c5cd33a620d/download9f5aeb3effe33790f2ed1e0b2c5ab4d9MD53Polymorphisms in Plasmodium vivax circumsporozoite protein (CSP) influence parasite burden and cytokine balance in a pre-amazon endemic area from Brazil.pdf.jpgPolymorphisms in Plasmodium vivax circumsporozoite protein (CSP) influence parasite burden and cytokine balance in a pre-amazon endemic area from Brazil.pdf.jpgGenerated Thumbnailimage/jpeg5545https://patua.iec.gov.br/bitstreams/d8e4c45d-65b1-45eb-b904-851b56177632/download7d1e67d24b300f2252cfd2fd1955db32MD58LICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv |
Polymorphisms in Plasmodium vivax circumsporozoite protein (CSP) influence parasite burden and cytokine balance in a pre-amazon endemic area from Brazil |
title |
Polymorphisms in Plasmodium vivax circumsporozoite protein (CSP) influence parasite burden and cytokine balance in a pre-amazon endemic area from Brazil |
spellingShingle |
Polymorphisms in Plasmodium vivax circumsporozoite protein (CSP) influence parasite burden and cytokine balance in a pre-amazon endemic area from Brazil Ribeiro, Bruno de Paulo Malária Vivax / parasitologia Plasmodium vivax / imunologia Plasmodium vivax / genética Polimorfismo Genético Polimorfismo de Fragmento de Restrição Variação Genética Genótipo Citocinas |
title_short |
Polymorphisms in Plasmodium vivax circumsporozoite protein (CSP) influence parasite burden and cytokine balance in a pre-amazon endemic area from Brazil |
title_full |
Polymorphisms in Plasmodium vivax circumsporozoite protein (CSP) influence parasite burden and cytokine balance in a pre-amazon endemic area from Brazil |
title_fullStr |
Polymorphisms in Plasmodium vivax circumsporozoite protein (CSP) influence parasite burden and cytokine balance in a pre-amazon endemic area from Brazil |
title_full_unstemmed |
Polymorphisms in Plasmodium vivax circumsporozoite protein (CSP) influence parasite burden and cytokine balance in a pre-amazon endemic area from Brazil |
title_sort |
Polymorphisms in Plasmodium vivax circumsporozoite protein (CSP) influence parasite burden and cytokine balance in a pre-amazon endemic area from Brazil |
author |
Ribeiro, Bruno de Paulo |
author_facet |
Ribeiro, Bruno de Paulo Cassiano, Gustavo Capatti Souza, Rodrigo Medeiros de Cysne, Dalila Nunes Grisotto, Marcos Augusto Grigolin Santos, Ana Paula Silva de Azevedo dos Marinho, Cláudio Romero Farias Machado, Ricardo Luiz Dantas Nascimento, Flávia Raquel Fernandes |
author_role |
author |
author2 |
Cassiano, Gustavo Capatti Souza, Rodrigo Medeiros de Cysne, Dalila Nunes Grisotto, Marcos Augusto Grigolin Santos, Ana Paula Silva de Azevedo dos Marinho, Cláudio Romero Farias Machado, Ricardo Luiz Dantas Nascimento, Flávia Raquel Fernandes |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Ribeiro, Bruno de Paulo Cassiano, Gustavo Capatti Souza, Rodrigo Medeiros de Cysne, Dalila Nunes Grisotto, Marcos Augusto Grigolin Santos, Ana Paula Silva de Azevedo dos Marinho, Cláudio Romero Farias Machado, Ricardo Luiz Dantas Nascimento, Flávia Raquel Fernandes |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Malária Vivax / parasitologia Plasmodium vivax / imunologia Plasmodium vivax / genética Polimorfismo Genético Polimorfismo de Fragmento de Restrição Variação Genética Genótipo Citocinas |
topic |
Malária Vivax / parasitologia Plasmodium vivax / imunologia Plasmodium vivax / genética Polimorfismo Genético Polimorfismo de Fragmento de Restrição Variação Genética Genótipo Citocinas |
description |
Mechanisms involved in severe P. vivax malaria remain unclear. Parasite polymorphisms, parasite load and host cytokine profile may influence the course of infection. In this study, we investigated the influence of circumsporozoite protein (CSP) polymorphisms on parasite load and cytokine profile in patients with vivax malaria. A cross-sectional study was carried out in three cities: São Luís, Cedral and Buriticupu, Maranhão state, Brazil, areas of high prevalence of P. vivax. Interleukin (IL)-2, IL-4, IL-10, IL-6, IL-17, tumor necrosis factor alpha (TNF-α, interferon gamma (IFN-γ and transforming growth factor beta (TGF-β were quantified in blood plasma of patients and in supernatants from peripheral blood mononuclear cell (PBMC) cultures. Furthermore, the levels of cytokines and parasite load were correlated with VK210, VK247 and P. vivax-like CSP variants. Patients infected with P. vivax showed increased IL-10 and IL-6 levels, which correlated with the parasite load, however, in multiple comparisons, only IL-10 kept this association. A regulatory cytokine profile prevailed in plasma, while an inflammatory profile prevailed in PBMC culture supernatants and these patterns were related to CSP polymorphisms. VK247 infected patients showed higher parasitaemia and IL-6 concentrations, which were not associated to IL-10 anti-inflammatory effect. By contrast, in VK210 patients, these two cytokines showed a strong positive correlation and the parasite load was lower. Patients with the VK210 variant showed a regulatory cytokine profile in plasma, while those infected with the VK247 variant have a predominantly inflammatory cytokine profile and higher parasite loads, which altogether may result in more complications in infection. In conclusion, we propose that CSP polymorphisms is associated to the increase of non-regulated inflammatory immune responses, which in turn may be associated with the outcome of infection. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016 |
dc.date.accessioned.fl_str_mv |
2017-03-24T11:54:20Z |
dc.date.available.fl_str_mv |
2017-03-24T11:54:20Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
RIBEIRO, Bruno de Paulo et al. Polymorphisms in Plasmodium vivax circumsporozoite protein (CSP) influence parasite burden and cytokine balance in a pre-amazon endemic area from Brazil. PLoS Neglected Tropical Diseases, v. 10, n. 3, p. 1-9, 2016. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/2430 |
dc.identifier.issn.-.fl_str_mv |
1935-2735 |
dc.identifier.doi.-.fl_str_mv |
10.1371/journal.pntd.0004479 |
identifier_str_mv |
RIBEIRO, Bruno de Paulo et al. Polymorphisms in Plasmodium vivax circumsporozoite protein (CSP) influence parasite burden and cytokine balance in a pre-amazon endemic area from Brazil. PLoS Neglected Tropical Diseases, v. 10, n. 3, p. 1-9, 2016. 1935-2735 10.1371/journal.pntd.0004479 |
url |
https://patua.iec.gov.br/handle/iec/2430 |
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eng |
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eng |
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openAccess |
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Public Library of Science |
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Public Library of Science |
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