Triatomine and Trypanosoma cruzi discrete typing units distribution in a semi-arid area of northeastern Brazil

Detalhes bibliográficos
Autor(a) principal: Honorato, Nathan Ravi Medeiros
Data de Publicação: 2021
Outros Autores: Silva, Andress Noronha Barbosa da, Negreiros, Christiane Carlos Araújo de, Aguiar, Lúcia Maria Abrantes, Marliére, Newmar Pinto, Souza, Rita de Cássia Moreira de, Guimarães, Ricardo José de Paula Souza e, Galvão, Lúcia Maria da Cunha, Câmara, Antônia Cláudia Jácome da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
Texto Completo: https://patua.iec.gov.br/handle/iec/4314
Resumo: The occurrence of triatomine species, their bloodmeal sources and the discrete typing units (DTUs) of Trypanosoma cruzi isolated from them were determined in different municipalities of the state of Rio Grande do Norte, Brazil. Triatomine captures were carried out in the rural areas of 23 municipalities. The genotyping of T. cruzi isolates was performed using the mitochondrial cytochrome c oxidase subunit 2 (coii) gene, the D7 region of the 24Sα rDNA, and the spliced leader intergenic region (SL-IR). Five triatomine species were captured, and the most frequent was Triatoma brasiliensis (84.3%; 916/1086), which was found in 16 of the 23 municipalities surveyed, and infested all types of environment investigated. The TcI DTU was found in all mesoregions surveyed in 51.5% (17/33) of the culture-positive samples. In contrast, TcII (9.1%; 3/33) was detected in the Central mesoregion, while TcIII (27.3%; 9/33) was found in all mesoregions. The geographic distribution and spatial overlap of different DTUs was inferred using the superposition of the radius of occurrence of isolates and using ecological niche distribution modelling. Triatoma brasiliensis was found infected in all mesoregions and with all three T. cruzi DTUs, including mixed infections. With regard to bloodmeal sources, the DNA of rodents was found in triatomines infected with either TcI or TcIII, while that of domestic animals and humans was associated with both single and mixed infections. Our findings demonstrate that different DTUs of T. cruzi are widely dispersed among triatomines in our study area. The association of T. brasiliensis with several different mammalian hosts, as well as overlapping areas with different DTUs, suggests that this triatomine species may have an important role as a vector in both anthropic and sylvatic environments.
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spelling Honorato, Nathan Ravi MedeirosSilva, Andress Noronha Barbosa daNegreiros, Christiane Carlos Araújo deAguiar, Lúcia Maria AbrantesMarliére, Newmar PintoSouza, Rita de Cássia Moreira deGuimarães, Ricardo José de Paula Souza eGalvão, Lúcia Maria da CunhaCâmara, Antônia Cláudia Jácome da2021-05-24T18:54:15Z2021-05-24T18:54:15Z2021HONORATO, Nathan Ravi Medeiros et al. Triatomine and Trypanosoma cruzi discrete typing units distribution in a semi-arid area of northeastern Brazil. Acta Tropica, v. 220, n. xx. p. xx, Aug. 2021.DOI: https://doi.org/10.1016/j.actatropica.2021.105950.0001-706Xhttps://patua.iec.gov.br/handle/iec/431410.1016/j.actatropica.2021.105950The occurrence of triatomine species, their bloodmeal sources and the discrete typing units (DTUs) of Trypanosoma cruzi isolated from them were determined in different municipalities of the state of Rio Grande do Norte, Brazil. Triatomine captures were carried out in the rural areas of 23 municipalities. The genotyping of T. cruzi isolates was performed using the mitochondrial cytochrome c oxidase subunit 2 (coii) gene, the D7 region of the 24Sα rDNA, and the spliced leader intergenic region (SL-IR). Five triatomine species were captured, and the most frequent was Triatoma brasiliensis (84.3%; 916/1086), which was found in 16 of the 23 municipalities surveyed, and infested all types of environment investigated. The TcI DTU was found in all mesoregions surveyed in 51.5% (17/33) of the culture-positive samples. In contrast, TcII (9.1%; 3/33) was detected in the Central mesoregion, while TcIII (27.3%; 9/33) was found in all mesoregions. The geographic distribution and spatial overlap of different DTUs was inferred using the superposition of the radius of occurrence of isolates and using ecological niche distribution modelling. Triatoma brasiliensis was found infected in all mesoregions and with all three T. cruzi DTUs, including mixed infections. With regard to bloodmeal sources, the DNA of rodents was found in triatomines infected with either TcI or TcIII, while that of domestic animals and humans was associated with both single and mixed infections. Our findings demonstrate that different DTUs of T. cruzi are widely dispersed among triatomines in our study area. The association of T. brasiliensis with several different mammalian hosts, as well as overlapping areas with different DTUs, suggests that this triatomine species may have an important role as a vector in both anthropic and sylvatic environments.Federal University of Rio Grande do Norte. Graduate Program in Parasitic Biology. Natal, RN, Brazil.Federal University of Rio Grande do Norte. Graduate Program in Pharmaceutical Sciences. Natal, RN, Brazil.Federal University of Rio Grande do Norte. Pharmacy Undergraduate Course. Natal, RN, Brazil.State Secretariat of Public Health of Rio Grande do Norte. Natal, RN, Brazil.Fundação Oswaldo Cruz. René Rachou Institute. Triatomine Research Group. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. René Rachou Institute. Triatomine Research Group. Belo Horizonte, MG, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Geoprocessamento. Ananindeua, PA, Brasil.Federal University of Rio Grande do Norte. Graduate Program in Pharmaceutical Sciences. Natal, RN, Brazil / Federal University of Rio Grande do Norte. Graduate Program in Health Sciences. Natal, RN, Brazil.Federal University of Rio Grande do Norte. Graduate Program in Parasitic Biology. Natal, RN, Brazil / Federal University of Rio Grande do Norte. Graduate Program in Pharmaceutical Sciences. Natal, RN, Brazil.engElsevierTriatomine and Trypanosoma cruzi discrete typing units distribution in a semi-arid area of northeastern Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleDoença de ChagasTrypanosoma cruzi / parasitologiaTriatominae / anatomia & histologiaVariação GenéticaComportamento AlimentarCoinfecção / sangueZona RuralRio Grande do Norte (BR)info:eu-repo/semantics/embargoedAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALTriatomine and Trypanosoma cruzi discrete typing units distribution in a semi-arid area of northeastern Brazil.pdfTriatomine and Trypanosoma cruzi discrete typing units distribution in a semi-arid area of northeastern Brazil.pdfapplication/pdf551083https://patua.iec.gov.br/bitstreams/6fc8decf-24fa-42b4-b6f3-6ccfa3bfddb1/downloadc9a9c128e29cac82a5d7fdf3f4e6da73MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82182https://patua.iec.gov.br/bitstreams/65751c88-6d4a-4400-86c3-606f6002fbbd/download11832eea31b16df8613079d742d61793MD52TEXTTriatomine and Trypanosoma cruzi discrete typing units distribution in a semi-arid area of northeastern Brazil.pdf.txtTriatomine and Trypanosoma cruzi discrete typing units distribution in a semi-arid area of northeastern Brazil.pdf.txtExtracted texttext/plain2https://patua.iec.gov.br/bitstreams/e0811046-4f46-46ed-91a7-075091b6b576/downloade1c06d85ae7b8b032bef47e42e4c08f9MD55THUMBNAILTriatomine and Trypanosoma cruzi discrete typing units distribution in a semi-arid area of northeastern Brazil.pdf.jpgTriatomine and Trypanosoma cruzi discrete typing units distribution in a semi-arid area of northeastern Brazil.pdf.jpgGenerated Thumbnailimage/jpeg3095https://patua.iec.gov.br/bitstreams/039385c7-e978-4b67-a029-351c95d18f6d/download71859d578212107f7f8c49a4ce09d9eeMD56iec/43142022-10-20 21:17:27.25oai:patua.iec.gov.br:iec/4314https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-20T21:17:27Repositório Digital do Instituto Evandro Chagas (Patuá) - 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dc.title.pt_BR.fl_str_mv Triatomine and Trypanosoma cruzi discrete typing units distribution in a semi-arid area of northeastern Brazil
title Triatomine and Trypanosoma cruzi discrete typing units distribution in a semi-arid area of northeastern Brazil
spellingShingle Triatomine and Trypanosoma cruzi discrete typing units distribution in a semi-arid area of northeastern Brazil
Honorato, Nathan Ravi Medeiros
Doença de Chagas
Trypanosoma cruzi / parasitologia
Triatominae / anatomia & histologia
Variação Genética
Comportamento Alimentar
Coinfecção / sangue
Zona Rural
Rio Grande do Norte (BR)
title_short Triatomine and Trypanosoma cruzi discrete typing units distribution in a semi-arid area of northeastern Brazil
title_full Triatomine and Trypanosoma cruzi discrete typing units distribution in a semi-arid area of northeastern Brazil
title_fullStr Triatomine and Trypanosoma cruzi discrete typing units distribution in a semi-arid area of northeastern Brazil
title_full_unstemmed Triatomine and Trypanosoma cruzi discrete typing units distribution in a semi-arid area of northeastern Brazil
title_sort Triatomine and Trypanosoma cruzi discrete typing units distribution in a semi-arid area of northeastern Brazil
author Honorato, Nathan Ravi Medeiros
author_facet Honorato, Nathan Ravi Medeiros
Silva, Andress Noronha Barbosa da
Negreiros, Christiane Carlos Araújo de
Aguiar, Lúcia Maria Abrantes
Marliére, Newmar Pinto
Souza, Rita de Cássia Moreira de
Guimarães, Ricardo José de Paula Souza e
Galvão, Lúcia Maria da Cunha
Câmara, Antônia Cláudia Jácome da
author_role author
author2 Silva, Andress Noronha Barbosa da
Negreiros, Christiane Carlos Araújo de
Aguiar, Lúcia Maria Abrantes
Marliére, Newmar Pinto
Souza, Rita de Cássia Moreira de
Guimarães, Ricardo José de Paula Souza e
Galvão, Lúcia Maria da Cunha
Câmara, Antônia Cláudia Jácome da
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Honorato, Nathan Ravi Medeiros
Silva, Andress Noronha Barbosa da
Negreiros, Christiane Carlos Araújo de
Aguiar, Lúcia Maria Abrantes
Marliére, Newmar Pinto
Souza, Rita de Cássia Moreira de
Guimarães, Ricardo José de Paula Souza e
Galvão, Lúcia Maria da Cunha
Câmara, Antônia Cláudia Jácome da
dc.subject.decsPrimary.pt_BR.fl_str_mv Doença de Chagas
Trypanosoma cruzi / parasitologia
Triatominae / anatomia & histologia
Variação Genética
Comportamento Alimentar
Coinfecção / sangue
Zona Rural
Rio Grande do Norte (BR)
topic Doença de Chagas
Trypanosoma cruzi / parasitologia
Triatominae / anatomia & histologia
Variação Genética
Comportamento Alimentar
Coinfecção / sangue
Zona Rural
Rio Grande do Norte (BR)
description The occurrence of triatomine species, their bloodmeal sources and the discrete typing units (DTUs) of Trypanosoma cruzi isolated from them were determined in different municipalities of the state of Rio Grande do Norte, Brazil. Triatomine captures were carried out in the rural areas of 23 municipalities. The genotyping of T. cruzi isolates was performed using the mitochondrial cytochrome c oxidase subunit 2 (coii) gene, the D7 region of the 24Sα rDNA, and the spliced leader intergenic region (SL-IR). Five triatomine species were captured, and the most frequent was Triatoma brasiliensis (84.3%; 916/1086), which was found in 16 of the 23 municipalities surveyed, and infested all types of environment investigated. The TcI DTU was found in all mesoregions surveyed in 51.5% (17/33) of the culture-positive samples. In contrast, TcII (9.1%; 3/33) was detected in the Central mesoregion, while TcIII (27.3%; 9/33) was found in all mesoregions. The geographic distribution and spatial overlap of different DTUs was inferred using the superposition of the radius of occurrence of isolates and using ecological niche distribution modelling. Triatoma brasiliensis was found infected in all mesoregions and with all three T. cruzi DTUs, including mixed infections. With regard to bloodmeal sources, the DNA of rodents was found in triatomines infected with either TcI or TcIII, while that of domestic animals and humans was associated with both single and mixed infections. Our findings demonstrate that different DTUs of T. cruzi are widely dispersed among triatomines in our study area. The association of T. brasiliensis with several different mammalian hosts, as well as overlapping areas with different DTUs, suggests that this triatomine species may have an important role as a vector in both anthropic and sylvatic environments.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-05-24T18:54:15Z
dc.date.available.fl_str_mv 2021-05-24T18:54:15Z
dc.date.issued.fl_str_mv 2021
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dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv HONORATO, Nathan Ravi Medeiros et al. Triatomine and Trypanosoma cruzi discrete typing units distribution in a semi-arid area of northeastern Brazil. Acta Tropica, v. 220, n. xx. p. xx, Aug. 2021.DOI: https://doi.org/10.1016/j.actatropica.2021.105950.
dc.identifier.uri.fl_str_mv https://patua.iec.gov.br/handle/iec/4314
dc.identifier.issn.-.fl_str_mv 0001-706X
dc.identifier.doi.pt_BR.fl_str_mv 10.1016/j.actatropica.2021.105950
identifier_str_mv HONORATO, Nathan Ravi Medeiros et al. Triatomine and Trypanosoma cruzi discrete typing units distribution in a semi-arid area of northeastern Brazil. Acta Tropica, v. 220, n. xx. p. xx, Aug. 2021.DOI: https://doi.org/10.1016/j.actatropica.2021.105950.
0001-706X
10.1016/j.actatropica.2021.105950
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