Reduced Leishmania (L.) infantum chagasi parasitic loads in humans exposed to Lutzomyia longipalpis bites in the Amazon region of Brazil
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/3699 |
Resumo: | We performed a cross-sectional survey in humans to evaluate Lutzomyia longipalpis, i.e. sand fly vector, bite exposure association with Leishmania (Leishmania) infantum chagasi infection in Bujarú municipality, Northern Brazil, an endemic area for visceral leishmaniasis. In recruited individuals, which were stratified by sex and age, we measured L. (L.) i. chagasi parasitic loads with quantitative polymerase chain reaction (qPCR), exposure to sand fly bites with an anti-saliva immunoglobulin G enzyme-linked immunosorbent assay and performed immunological diagnostic tests, in order to evaluate the association between exposure to sand fly bites, and infection. The prevalence increased from 11% when using immunological diagnostic tests to 28% when using qPCR, being around that value for all age classes, but children below 5 years (40%) and people over 60 years (15%). The association between PCR-based L. (L.) i. chagasi prevalence and saliva exposure was convex, reflecting the fact that at both high and low saliva exposure the PCR-based L. (L.) i. chagasi prevalence decreases. This scenario indicates that low sand fly exposure is likely associated with low parasite transmission, while high anti-saliva prevalence, i.e. a large sand fly bite exposure could be associated with anti-Leishmania protective immune mechanisms driven by vector saliva and/or increased parasite exposure |
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Laurenti, Márcia DalastraChaves, Luis FernandoTomokane, Thaíse YumieAbbasi, IbrahimWarburg, AlonSilveira, Fernando Tobias2019-05-17T18:13:40Z2019-05-17T18:13:40Z2017LAURENTI, Márcia Dalastra et al. Reduced Leishmania (L.) infantum chagasi parasitic loads in humans exposed to Lutzomyia longipalpis bites in the Amazon region of Brazil. Parasitology Open, v. 3, n. 2, p. 1-10, Mar. 2017. DOI: https://doi.org/10.1017/pao.2017.2. Disponível em: https://www.cambridge.org/core/journals/parasitology-open/article/reduced-leishmania-l-infantum-chagasi-parasitic-loads-in-humans-exposed-to-lutzomyia-longipalpis-bites-in-the-amazon-region-of-brazil/CC0043B4406C6EF290B44478E6DBD386.2055-7094https://patua.iec.gov.br/handle/iec/369910.1017/pao.2017.2We performed a cross-sectional survey in humans to evaluate Lutzomyia longipalpis, i.e. sand fly vector, bite exposure association with Leishmania (Leishmania) infantum chagasi infection in Bujarú municipality, Northern Brazil, an endemic area for visceral leishmaniasis. In recruited individuals, which were stratified by sex and age, we measured L. (L.) i. chagasi parasitic loads with quantitative polymerase chain reaction (qPCR), exposure to sand fly bites with an anti-saliva immunoglobulin G enzyme-linked immunosorbent assay and performed immunological diagnostic tests, in order to evaluate the association between exposure to sand fly bites, and infection. The prevalence increased from 11% when using immunological diagnostic tests to 28% when using qPCR, being around that value for all age classes, but children below 5 years (40%) and people over 60 years (15%). The association between PCR-based L. (L.) i. chagasi prevalence and saliva exposure was convex, reflecting the fact that at both high and low saliva exposure the PCR-based L. (L.) i. chagasi prevalence decreases. This scenario indicates that low sand fly exposure is likely associated with low parasite transmission, while high anti-saliva prevalence, i.e. a large sand fly bite exposure could be associated with anti-Leishmania protective immune mechanisms driven by vector saliva and/or increased parasite exposureSão Paulo Research Foundation-Hebrew University of Jerusalem (FAPESP-HUJ) collaborative grant #2011/ 51404-9, Brazilian Ministry of Health and LIM50 HCFMUSP. M.D.L. is a Research Fellows from CNPq, Brazil. L.F.C. was funded by Nagasaki University (Program for Nurturing Global Leaders in Tropical and Emerging Communicable Diseases).University of São Paulo. Medical School. Department of Pathology. Laboratory of Pathology of Infectious Diseases. São Paulo, SP, Brazil.Universidad de Costa Rica. Centro de Investigación en Enfermedades Tropicales. San Pedro de Montes de Oca, Costa Rica / Universidad Nacional. Escuela de Medicina Veterinaria. Programa de Investigación en Enfermedades Tropicales. Heredia, Costa Rica / Nagasaki University. Institute of Tropical Medicine. Nagasaki, Japan.University of São Paulo. Medical School. Department of Pathology. Laboratory of Pathology of Infectious Diseases. São Paulo, SP, Brazil.The University of Jerusalem. The Institute of Medical Research Israel-Canada. The Kuvin Center for the study of Infectious and Tropical Diseases. The Department of Microbiology and Molecular Genetics. Jerusalem, Israel.The University of Jerusalem. The Institute of Medical Research Israel-Canada. The Kuvin Center for the study of Infectious and Tropical Diseases. The Department of Microbiology and Molecular Genetics. Jerusalem, Israel.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Federal University of Pará. Nucleous of Tropical Medicine. Belém, PA, Brazil.engCambridge University PressReduced Leishmania (L.) infantum chagasi parasitic loads in humans exposed to Lutzomyia longipalpis bites in the Amazon region of Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLeishmaniose Visceral / parasitologiaInfecções por Protozoários / parasitologiaPsychodidae / parasitologiaVetores de DoençasLeishmaniose / parasitologiaTécnicas Imunoenzimáticas / métodosBujarú (PA)info:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALReduced Leishmania (L.) infantum chagasi parasitic loads in humans exposed to Lutzomyia longipalpis bites in the Amazon region of Brazil.pdfReduced Leishmania (L.) infantum chagasi parasitic loads in humans exposed to Lutzomyia longipalpis bites in the Amazon region of Brazil.pdfapplication/pdf314533https://patua.iec.gov.br/bitstreams/84bf2a74-f8e7-4b3e-a2c9-aff3ac9ce79a/download4811868b0c7dd5546d5bc3d9a0ff7c4dMD51LICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv |
Reduced Leishmania (L.) infantum chagasi parasitic loads in humans exposed to Lutzomyia longipalpis bites in the Amazon region of Brazil |
title |
Reduced Leishmania (L.) infantum chagasi parasitic loads in humans exposed to Lutzomyia longipalpis bites in the Amazon region of Brazil |
spellingShingle |
Reduced Leishmania (L.) infantum chagasi parasitic loads in humans exposed to Lutzomyia longipalpis bites in the Amazon region of Brazil Laurenti, Márcia Dalastra Leishmaniose Visceral / parasitologia Infecções por Protozoários / parasitologia Psychodidae / parasitologia Vetores de Doenças Leishmaniose / parasitologia Técnicas Imunoenzimáticas / métodos Bujarú (PA) |
title_short |
Reduced Leishmania (L.) infantum chagasi parasitic loads in humans exposed to Lutzomyia longipalpis bites in the Amazon region of Brazil |
title_full |
Reduced Leishmania (L.) infantum chagasi parasitic loads in humans exposed to Lutzomyia longipalpis bites in the Amazon region of Brazil |
title_fullStr |
Reduced Leishmania (L.) infantum chagasi parasitic loads in humans exposed to Lutzomyia longipalpis bites in the Amazon region of Brazil |
title_full_unstemmed |
Reduced Leishmania (L.) infantum chagasi parasitic loads in humans exposed to Lutzomyia longipalpis bites in the Amazon region of Brazil |
title_sort |
Reduced Leishmania (L.) infantum chagasi parasitic loads in humans exposed to Lutzomyia longipalpis bites in the Amazon region of Brazil |
author |
Laurenti, Márcia Dalastra |
author_facet |
Laurenti, Márcia Dalastra Chaves, Luis Fernando Tomokane, Thaíse Yumie Abbasi, Ibrahim Warburg, Alon Silveira, Fernando Tobias |
author_role |
author |
author2 |
Chaves, Luis Fernando Tomokane, Thaíse Yumie Abbasi, Ibrahim Warburg, Alon Silveira, Fernando Tobias |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Laurenti, Márcia Dalastra Chaves, Luis Fernando Tomokane, Thaíse Yumie Abbasi, Ibrahim Warburg, Alon Silveira, Fernando Tobias |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Leishmaniose Visceral / parasitologia Infecções por Protozoários / parasitologia Psychodidae / parasitologia Vetores de Doenças Leishmaniose / parasitologia Técnicas Imunoenzimáticas / métodos Bujarú (PA) |
topic |
Leishmaniose Visceral / parasitologia Infecções por Protozoários / parasitologia Psychodidae / parasitologia Vetores de Doenças Leishmaniose / parasitologia Técnicas Imunoenzimáticas / métodos Bujarú (PA) |
description |
We performed a cross-sectional survey in humans to evaluate Lutzomyia longipalpis, i.e. sand fly vector, bite exposure association with Leishmania (Leishmania) infantum chagasi infection in Bujarú municipality, Northern Brazil, an endemic area for visceral leishmaniasis. In recruited individuals, which were stratified by sex and age, we measured L. (L.) i. chagasi parasitic loads with quantitative polymerase chain reaction (qPCR), exposure to sand fly bites with an anti-saliva immunoglobulin G enzyme-linked immunosorbent assay and performed immunological diagnostic tests, in order to evaluate the association between exposure to sand fly bites, and infection. The prevalence increased from 11% when using immunological diagnostic tests to 28% when using qPCR, being around that value for all age classes, but children below 5 years (40%) and people over 60 years (15%). The association between PCR-based L. (L.) i. chagasi prevalence and saliva exposure was convex, reflecting the fact that at both high and low saliva exposure the PCR-based L. (L.) i. chagasi prevalence decreases. This scenario indicates that low sand fly exposure is likely associated with low parasite transmission, while high anti-saliva prevalence, i.e. a large sand fly bite exposure could be associated with anti-Leishmania protective immune mechanisms driven by vector saliva and/or increased parasite exposure |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017 |
dc.date.accessioned.fl_str_mv |
2019-05-17T18:13:40Z |
dc.date.available.fl_str_mv |
2019-05-17T18:13:40Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
LAURENTI, Márcia Dalastra et al. Reduced Leishmania (L.) infantum chagasi parasitic loads in humans exposed to Lutzomyia longipalpis bites in the Amazon region of Brazil. Parasitology Open, v. 3, n. 2, p. 1-10, Mar. 2017. DOI: https://doi.org/10.1017/pao.2017.2. Disponível em: https://www.cambridge.org/core/journals/parasitology-open/article/reduced-leishmania-l-infantum-chagasi-parasitic-loads-in-humans-exposed-to-lutzomyia-longipalpis-bites-in-the-amazon-region-of-brazil/CC0043B4406C6EF290B44478E6DBD386. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/3699 |
dc.identifier.issn.-.fl_str_mv |
2055-7094 |
dc.identifier.doi.pt_BR.fl_str_mv |
10.1017/pao.2017.2 |
identifier_str_mv |
LAURENTI, Márcia Dalastra et al. Reduced Leishmania (L.) infantum chagasi parasitic loads in humans exposed to Lutzomyia longipalpis bites in the Amazon region of Brazil. Parasitology Open, v. 3, n. 2, p. 1-10, Mar. 2017. DOI: https://doi.org/10.1017/pao.2017.2. Disponível em: https://www.cambridge.org/core/journals/parasitology-open/article/reduced-leishmania-l-infantum-chagasi-parasitic-loads-in-humans-exposed-to-lutzomyia-longipalpis-bites-in-the-amazon-region-of-brazil/CC0043B4406C6EF290B44478E6DBD386. 2055-7094 10.1017/pao.2017.2 |
url |
https://patua.iec.gov.br/handle/iec/3699 |
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eng |
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eng |
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Cambridge University Press |
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Cambridge University Press |
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