Mitochondrial genome sequencing and phylogeny of Haemagogus albomaculatus, Haemagogus leucocelaenus, Haemagogus spegazzinii, and Haemagogus tropicalis (Diptera: Culicidae)
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/4189 |
Resumo: | The genus Haemagogus (Diptera: Culicidae) comprises species of great epidemiological relevance, involved in transmission cycles of the Yellow fever virus and other arboviruses in South America. So far, only Haemagogus janthinomys has complete mitochondrial sequences available. Given the unavailability of information related to aspects of the evolutionary biology and molecular taxonomy of this genus, we report here, the first sequencing of the mitogenomes of Haemagogus albomaculatus, Haemagogus leucocelaenus, Haemagogus spegazzinii, and Haemagogus tropicalis. The mitogenomes showed an average length of 15,038 bp, average AT content of 79.3%, positive AT-skews, negative GC-skews, and comprised 37 functional subunits (13 PCGs, 22 tRNA, and 02 rRNA). The PCGs showed ATN as start codon, TAA as stop codon, and signs of purifying selection. The tRNAs had the typical leaf clover structure, except tRNASer1. Phylogenetic analyzes of Bayesian inference and Maximum Likelihood, based on concatenated sequences from all 13 PCGs, produced identical topologies and strongly supported the monophyletic relationship between the Haemagogus and Conopostegus subgenera, and corroborated with the known taxonomic classification of the evaluated taxa, based on external morphological aspects. The information produced on the mitogenomes of the Haemagogus species evaluated here may be useful in carrying out future taxonomic and evolutionary studies of the genus. |
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Silva, Fábio Silva daCruz, Ana Cecília RibeiroMedeiros, Daniele Barbosa de AlmeidaSilva, Sandro Patroca daNunes, Márcio Roberto TeixeiraMartins, Lívia CaricioChiang, Jannifer OliveiraLemos, Poliana da SilvaCunha, Gabriel MuricyAraujo, Renato Freitas deMonteiro, Hamilton Antônio de OliveiraNunes Neto, Joaquim Pinto2020-10-26T14:07:57Z2020-10-26T14:07:57Z2020SILVA, Fábio Silva da et al. Mitochondrial genome sequencing and phylogeny of Haemagogus albomaculatus, Haemagogus leucocelaenus, Haemagogus spegazzinii, and Haemagogus tropicalis (Diptera: Culicidae). Scientific Reports, v. 10, n. 1, p. 1-13, Dec. 2020.2045-2322https://patua.iec.gov.br/handle/iec/418910.1038/s41598-020-73790-xThe genus Haemagogus (Diptera: Culicidae) comprises species of great epidemiological relevance, involved in transmission cycles of the Yellow fever virus and other arboviruses in South America. So far, only Haemagogus janthinomys has complete mitochondrial sequences available. Given the unavailability of information related to aspects of the evolutionary biology and molecular taxonomy of this genus, we report here, the first sequencing of the mitogenomes of Haemagogus albomaculatus, Haemagogus leucocelaenus, Haemagogus spegazzinii, and Haemagogus tropicalis. The mitogenomes showed an average length of 15,038 bp, average AT content of 79.3%, positive AT-skews, negative GC-skews, and comprised 37 functional subunits (13 PCGs, 22 tRNA, and 02 rRNA). The PCGs showed ATN as start codon, TAA as stop codon, and signs of purifying selection. The tRNAs had the typical leaf clover structure, except tRNASer1. Phylogenetic analyzes of Bayesian inference and Maximum Likelihood, based on concatenated sequences from all 13 PCGs, produced identical topologies and strongly supported the monophyletic relationship between the Haemagogus and Conopostegus subgenera, and corroborated with the known taxonomic classification of the evaluated taxa, based on external morphological aspects. The information produced on the mitogenomes of the Haemagogus species evaluated here may be useful in carrying out future taxonomic and evolutionary studies of the genus.Evandro Chagas Institute (IEC/MS/SVS); Coordination for the Improvement of Higher Education Personnel (CAPES/1743623).State University of Pará. Center of Biological and Health Sciences. Graduate Program in Parasitary Biology in the Amazon. Belém, PA, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Bahia State Department of Health. Directorate of Epidemiological Surveillance. Salvador, BA, Brazil.Bahia State Department of Health. Directorate of Epidemiological Surveillance. Salvador, BA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.engNature ResearchMitochondrial genome sequencing and phylogeny of Haemagogus albomaculatus, Haemagogus leucocelaenus, Haemagogus spegazzinii, and Haemagogus tropicalis (Diptera: Culicidae)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleDípteros / classificaçãoCulicidae / classificaçãoMitocôndrias / genéticaFilogeniaSequenciamento Completo do Genomainfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALMitochondrial genome sequencing and phylogeny of Haemagogus albomaculatus, Haemagogus leucocelaenus, Haemagogus spegazzinii, and Haemagogus tropicalis (Diptera: Culicidae) .pdfMitochondrial genome sequencing and phylogeny of Haemagogus albomaculatus, Haemagogus leucocelaenus, Haemagogus spegazzinii, and Haemagogus tropicalis (Diptera: Culicidae) .pdfapplication/pdf2160559https://patua.iec.gov.br/bitstreams/7fcc6e4a-08da-4d60-8878-3d951e8541a1/downloadcce4418d1b38f3e0fbeb78343e2b1970MD51LICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv |
Mitochondrial genome sequencing and phylogeny of Haemagogus albomaculatus, Haemagogus leucocelaenus, Haemagogus spegazzinii, and Haemagogus tropicalis (Diptera: Culicidae) |
title |
Mitochondrial genome sequencing and phylogeny of Haemagogus albomaculatus, Haemagogus leucocelaenus, Haemagogus spegazzinii, and Haemagogus tropicalis (Diptera: Culicidae) |
spellingShingle |
Mitochondrial genome sequencing and phylogeny of Haemagogus albomaculatus, Haemagogus leucocelaenus, Haemagogus spegazzinii, and Haemagogus tropicalis (Diptera: Culicidae) Silva, Fábio Silva da Dípteros / classificação Culicidae / classificação Mitocôndrias / genética Filogenia Sequenciamento Completo do Genoma |
title_short |
Mitochondrial genome sequencing and phylogeny of Haemagogus albomaculatus, Haemagogus leucocelaenus, Haemagogus spegazzinii, and Haemagogus tropicalis (Diptera: Culicidae) |
title_full |
Mitochondrial genome sequencing and phylogeny of Haemagogus albomaculatus, Haemagogus leucocelaenus, Haemagogus spegazzinii, and Haemagogus tropicalis (Diptera: Culicidae) |
title_fullStr |
Mitochondrial genome sequencing and phylogeny of Haemagogus albomaculatus, Haemagogus leucocelaenus, Haemagogus spegazzinii, and Haemagogus tropicalis (Diptera: Culicidae) |
title_full_unstemmed |
Mitochondrial genome sequencing and phylogeny of Haemagogus albomaculatus, Haemagogus leucocelaenus, Haemagogus spegazzinii, and Haemagogus tropicalis (Diptera: Culicidae) |
title_sort |
Mitochondrial genome sequencing and phylogeny of Haemagogus albomaculatus, Haemagogus leucocelaenus, Haemagogus spegazzinii, and Haemagogus tropicalis (Diptera: Culicidae) |
author |
Silva, Fábio Silva da |
author_facet |
Silva, Fábio Silva da Cruz, Ana Cecília Ribeiro Medeiros, Daniele Barbosa de Almeida Silva, Sandro Patroca da Nunes, Márcio Roberto Teixeira Martins, Lívia Caricio Chiang, Jannifer Oliveira Lemos, Poliana da Silva Cunha, Gabriel Muricy Araujo, Renato Freitas de Monteiro, Hamilton Antônio de Oliveira Nunes Neto, Joaquim Pinto |
author_role |
author |
author2 |
Cruz, Ana Cecília Ribeiro Medeiros, Daniele Barbosa de Almeida Silva, Sandro Patroca da Nunes, Márcio Roberto Teixeira Martins, Lívia Caricio Chiang, Jannifer Oliveira Lemos, Poliana da Silva Cunha, Gabriel Muricy Araujo, Renato Freitas de Monteiro, Hamilton Antônio de Oliveira Nunes Neto, Joaquim Pinto |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Silva, Fábio Silva da Cruz, Ana Cecília Ribeiro Medeiros, Daniele Barbosa de Almeida Silva, Sandro Patroca da Nunes, Márcio Roberto Teixeira Martins, Lívia Caricio Chiang, Jannifer Oliveira Lemos, Poliana da Silva Cunha, Gabriel Muricy Araujo, Renato Freitas de Monteiro, Hamilton Antônio de Oliveira Nunes Neto, Joaquim Pinto |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Dípteros / classificação Culicidae / classificação Mitocôndrias / genética Filogenia Sequenciamento Completo do Genoma |
topic |
Dípteros / classificação Culicidae / classificação Mitocôndrias / genética Filogenia Sequenciamento Completo do Genoma |
description |
The genus Haemagogus (Diptera: Culicidae) comprises species of great epidemiological relevance, involved in transmission cycles of the Yellow fever virus and other arboviruses in South America. So far, only Haemagogus janthinomys has complete mitochondrial sequences available. Given the unavailability of information related to aspects of the evolutionary biology and molecular taxonomy of this genus, we report here, the first sequencing of the mitogenomes of Haemagogus albomaculatus, Haemagogus leucocelaenus, Haemagogus spegazzinii, and Haemagogus tropicalis. The mitogenomes showed an average length of 15,038 bp, average AT content of 79.3%, positive AT-skews, negative GC-skews, and comprised 37 functional subunits (13 PCGs, 22 tRNA, and 02 rRNA). The PCGs showed ATN as start codon, TAA as stop codon, and signs of purifying selection. The tRNAs had the typical leaf clover structure, except tRNASer1. Phylogenetic analyzes of Bayesian inference and Maximum Likelihood, based on concatenated sequences from all 13 PCGs, produced identical topologies and strongly supported the monophyletic relationship between the Haemagogus and Conopostegus subgenera, and corroborated with the known taxonomic classification of the evaluated taxa, based on external morphological aspects. The information produced on the mitogenomes of the Haemagogus species evaluated here may be useful in carrying out future taxonomic and evolutionary studies of the genus. |
publishDate |
2020 |
dc.date.accessioned.fl_str_mv |
2020-10-26T14:07:57Z |
dc.date.available.fl_str_mv |
2020-10-26T14:07:57Z |
dc.date.issued.fl_str_mv |
2020 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
format |
article |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
SILVA, Fábio Silva da et al. Mitochondrial genome sequencing and phylogeny of Haemagogus albomaculatus, Haemagogus leucocelaenus, Haemagogus spegazzinii, and Haemagogus tropicalis (Diptera: Culicidae). Scientific Reports, v. 10, n. 1, p. 1-13, Dec. 2020. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/4189 |
dc.identifier.issn.-.fl_str_mv |
2045-2322 |
dc.identifier.doi.-.fl_str_mv |
10.1038/s41598-020-73790-x |
identifier_str_mv |
SILVA, Fábio Silva da et al. Mitochondrial genome sequencing and phylogeny of Haemagogus albomaculatus, Haemagogus leucocelaenus, Haemagogus spegazzinii, and Haemagogus tropicalis (Diptera: Culicidae). Scientific Reports, v. 10, n. 1, p. 1-13, Dec. 2020. 2045-2322 10.1038/s41598-020-73790-x |
url |
https://patua.iec.gov.br/handle/iec/4189 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Nature Research |
publisher.none.fl_str_mv |
Nature Research |
dc.source.none.fl_str_mv |
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