Some methods for membrane feeding of laboratory reared, neotropical sandflies (Diptera: Psychodidae)
Autor(a) principal: | |
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Data de Publicação: | 1978 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/788 |
Resumo: | A brief introduction to the history and applications of membrane feeding is given, together with its use to feed and infect sandflies with Leishmania. The present paper describes methods for feeding Lutzomyia longipalpis and Lu.flaviscutellata through membranes. Of the membranes tested, chick skins and bat wings gave the best results. Membranes stored at -20°C for over a year were found to be less effective than fresh ones. Blood meals were maintained at temperatures between 24°C and 37°C and it is concluded that although temperatures as high as 37°C are unnecessary for successful membrane feeding of sandflies, fewer flies feed in most instances at temperatures below 27°C. The influence on feeding of individual skins, different lots of blood and the addition of leishmanial cultures to offered blood are discussed. ACKNOWLEDGEMENTS. We are grateful to Srta's C. Loureiro, C. Nascimento, S. Silva and Sr. J. Almeida for laboratory assistance with the sandfly colonies, and to Sr. R. Naiff and Srta. M. Farias for maintaining the Leishmania cultures. Mr. J. Turner, glass blower ofthe London School of Hygiene and Tropical Medicine kindly prepared the two multiplefeeders. The work was carried out under the auspices of the Wellcome Trust, London, and the Instituto Evandro Chagas of the Fundação Serviços de Saúde Pública of Brazil. |
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Ward, Richard DouglasLainson, RalphShaw, Jeffrey Jon2016-01-26T11:36:51Z1978WARD, Richard D.; LAINSON, Ralph; SHAW, Jeffery Jon. Some methods for membrane feeding of laboratory reared, neotropical sandflies (Diptera: Psychodidae). Annals of Tropical Medicine and Parasitology, v. 72, n. 3, p. 269-276, 1978.0003-4983https://patua.iec.gov.br/handle/iec/788A brief introduction to the history and applications of membrane feeding is given, together with its use to feed and infect sandflies with Leishmania. The present paper describes methods for feeding Lutzomyia longipalpis and Lu.flaviscutellata through membranes. Of the membranes tested, chick skins and bat wings gave the best results. Membranes stored at -20°C for over a year were found to be less effective than fresh ones. Blood meals were maintained at temperatures between 24°C and 37°C and it is concluded that although temperatures as high as 37°C are unnecessary for successful membrane feeding of sandflies, fewer flies feed in most instances at temperatures below 27°C. The influence on feeding of individual skins, different lots of blood and the addition of leishmanial cultures to offered blood are discussed. ACKNOWLEDGEMENTS. We are grateful to Srta's C. Loureiro, C. Nascimento, S. Silva and Sr. J. Almeida for laboratory assistance with the sandfly colonies, and to Sr. R. Naiff and Srta. M. Farias for maintaining the Leishmania cultures. Mr. J. Turner, glass blower ofthe London School of Hygiene and Tropical Medicine kindly prepared the two multiplefeeders. The work was carried out under the auspices of the Wellcome Trust, London, and the Instituto Evandro Chagas of the Fundação Serviços de Saúde Pública of Brazil.Ministério da Saúde. Fundação Serviços de Saúde Pública. Instituto Evandro Chagas. Belém, PA, Brasil.Ministério da Saúde. Fundação Serviços de Saúde Pública. Instituto Evandro Chagas. Belém, PA, Brasil.Ministério da Saúde. Fundação Serviços de Saúde Pública. Instituto Evandro Chagas. Belém, PA, Brasil.application/pdfengTaylor & FrancisSome methods for membrane feeding of laboratory reared, neotropical sandflies (Diptera: Psychodidae)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePsychodidae / crescimento & desenvolvimentoPsychodidae / metabolismoLeishmania / patogenicidadeExperimentação Animalinfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALSome methods for membrane feeding of laboratory reared, neotropical sandflies (Diptera: Psychodidae) .pdfapplication/pdf1498544https://patua.iec.gov.br/bitstreams/eca8671e-65ad-4161-b1a3-4e51413044dd/download56bc59211bf1fa599a589aa136795bc2MD51TEXTfile_1.pdf.txtfile_1.pdf.txtExtracted texttext/plain21778https://patua.iec.gov.br/bitstreams/d9651b78-30d6-4395-8aaa-a7e2673b6524/download5dea788afb4d485cc338d559a4a3224aMD52Some methods for membrane feeding of laboratory reared, neotropical sandflies (Diptera: Psychodidae) .pdf.txtSome methods for membrane feeding of laboratory reared, neotropical sandflies (Diptera: Psychodidae) .pdf.txtExtracted texttext/plain22054https://patua.iec.gov.br/bitstreams/b3270ca5-400e-46cc-abcf-2184d23a7ed8/download8da40249328415a1074e962dfdd65979MD57THUMBNAILfile_1.pdf.jpgfile_1.pdf.jpgIM Thumbnailimage/jpeg4664https://patua.iec.gov.br/bitstreams/a884632d-8505-4650-8c47-332ec346f98e/download3c456b2600df5e920ab7a88fbb940a4bMD53Some methods for membrane feeding of laboratory reared, neotropical sandflies (Diptera: Psychodidae) .pdf.jpgSome methods for membrane feeding of laboratory reared, neotropical sandflies (Diptera: Psychodidae) .pdf.jpgGenerated Thumbnailimage/jpeg6162https://patua.iec.gov.br/bitstreams/171433ac-f1c0-4b2a-9e6c-c929179697a5/download62eac70c3fcfd2d14eb97e8852d7b8adMD58LICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv |
Some methods for membrane feeding of laboratory reared, neotropical sandflies (Diptera: Psychodidae) |
title |
Some methods for membrane feeding of laboratory reared, neotropical sandflies (Diptera: Psychodidae) |
spellingShingle |
Some methods for membrane feeding of laboratory reared, neotropical sandflies (Diptera: Psychodidae) Ward, Richard Douglas Psychodidae / crescimento & desenvolvimento Psychodidae / metabolismo Leishmania / patogenicidade Experimentação Animal |
title_short |
Some methods for membrane feeding of laboratory reared, neotropical sandflies (Diptera: Psychodidae) |
title_full |
Some methods for membrane feeding of laboratory reared, neotropical sandflies (Diptera: Psychodidae) |
title_fullStr |
Some methods for membrane feeding of laboratory reared, neotropical sandflies (Diptera: Psychodidae) |
title_full_unstemmed |
Some methods for membrane feeding of laboratory reared, neotropical sandflies (Diptera: Psychodidae) |
title_sort |
Some methods for membrane feeding of laboratory reared, neotropical sandflies (Diptera: Psychodidae) |
author |
Ward, Richard Douglas |
author_facet |
Ward, Richard Douglas Lainson, Ralph Shaw, Jeffrey Jon |
author_role |
author |
author2 |
Lainson, Ralph Shaw, Jeffrey Jon |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Ward, Richard Douglas Lainson, Ralph Shaw, Jeffrey Jon |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Psychodidae / crescimento & desenvolvimento Psychodidae / metabolismo Leishmania / patogenicidade Experimentação Animal |
topic |
Psychodidae / crescimento & desenvolvimento Psychodidae / metabolismo Leishmania / patogenicidade Experimentação Animal |
description |
A brief introduction to the history and applications of membrane feeding is given, together with its use to feed and infect sandflies with Leishmania. The present paper describes methods for feeding Lutzomyia longipalpis and Lu.flaviscutellata through membranes. Of the membranes tested, chick skins and bat wings gave the best results. Membranes stored at -20°C for over a year were found to be less effective than fresh ones. Blood meals were maintained at temperatures between 24°C and 37°C and it is concluded that although temperatures as high as 37°C are unnecessary for successful membrane feeding of sandflies, fewer flies feed in most instances at temperatures below 27°C. The influence on feeding of individual skins, different lots of blood and the addition of leishmanial cultures to offered blood are discussed. ACKNOWLEDGEMENTS. We are grateful to Srta's C. Loureiro, C. Nascimento, S. Silva and Sr. J. Almeida for laboratory assistance with the sandfly colonies, and to Sr. R. Naiff and Srta. M. Farias for maintaining the Leishmania cultures. Mr. J. Turner, glass blower ofthe London School of Hygiene and Tropical Medicine kindly prepared the two multiplefeeders. The work was carried out under the auspices of the Wellcome Trust, London, and the Instituto Evandro Chagas of the Fundação Serviços de Saúde Pública of Brazil. |
publishDate |
1978 |
dc.date.issued.fl_str_mv |
1978 |
dc.date.accessioned.fl_str_mv |
2016-01-26T11:36:51Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
WARD, Richard D.; LAINSON, Ralph; SHAW, Jeffery Jon. Some methods for membrane feeding of laboratory reared, neotropical sandflies (Diptera: Psychodidae). Annals of Tropical Medicine and Parasitology, v. 72, n. 3, p. 269-276, 1978. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/788 |
dc.identifier.issn.-.fl_str_mv |
0003-4983 |
identifier_str_mv |
WARD, Richard D.; LAINSON, Ralph; SHAW, Jeffery Jon. Some methods for membrane feeding of laboratory reared, neotropical sandflies (Diptera: Psychodidae). Annals of Tropical Medicine and Parasitology, v. 72, n. 3, p. 269-276, 1978. 0003-4983 |
url |
https://patua.iec.gov.br/handle/iec/788 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Taylor & Francis |
publisher.none.fl_str_mv |
Taylor & Francis |
dc.source.none.fl_str_mv |
reponame:Repositório Digital do Instituto Evandro Chagas (Patuá) instname:Instituto Evandro Chagas (IEC) instacron:IEC |
instname_str |
Instituto Evandro Chagas (IEC) |
instacron_str |
IEC |
institution |
IEC |
reponame_str |
Repositório Digital do Instituto Evandro Chagas (Patuá) |
collection |
Repositório Digital do Instituto Evandro Chagas (Patuá) |
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