Norovirus RNA in serum associated with increased fecal viral load in children: detection, quantification and molecular analysis.

• Autor(a) principal: Reymão, Tammy Kathlyn Amaral
• Data de Publicação: 2018
• Outros Autores: Fumian, Tulio Machado, Justino, Maria Cleonice Aguiar, Hernandez, Juliana Merces, Bandeira, Renato Silva, Lucena, Maria Silvia Sousa, Teixeira, Dielle Monteiro, Farias, Fredison Pinheiro, Silva, Luciana Damascena, Linhares, Alexandre da Costa, Gabbay, Yvone Benchimol
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
• Texto Completo: https://patua.iec.gov.br/handle/iec/3317
• topic: Norovirus / patogenicidade; Infecções por Caliciviridae / virologia; Gastroenterite / virologia; Variação Genética / genética; Monitoramento Epidemiológico; Reação em Cadeia da Polimerase / métodos; Ensaio de Imunoadsorção Enzimática / utilização; Fezes / virologia

Worldwide, norovirus (NoV) is a major cause of acute gastroenteritis (AGE) responsible for pandemics every ~3 years, and over 200,000 deaths per year, with the majority in children from developing countries. We investigate the incidence of NoV in children hospitalized with AGE from Belém, Pará, Brazil, and also correlated viral RNA levels in their blood and stool with clinical severity. For this purpose, paired stool and serum samples were collected from 445 pediatric patients, ≤9 years between March 2012 and June 2015. Enzyme-linked immunosorbent assay (EIA) was used to detect NoV in stool and reverse transcription quantitative PCR (RT-qPCR) used to quantify NoV RNA levels in sera (RNAemia) and in the positive stool. Positives samples were characterized by the partial ORF1/2 region sequence of viral genome. NoV antigen was detected in 24.3% (108/445) of stool samples, with RNAemia also present in 20.4% (22/108). RNAemia and a high stool viral load (>107 genome copies/gram of faeces) were associated with longer hospitalizations. The prevalent genotypes were GII.4 Sydney_2012 (71.6%-58/81) and New Orleans_2009 (6.2%-5/81) variants. Eight other genotypes belonging to GII were detected and four of them were recombinant strains. All sera were characterized as GII.4 and shared 100% similarity with their stool. The results suggest that the dissemination of NoV to the blood stream is not uncommon and may be related to increased faecal viral loads and disease severity.