Field and classroom initiatives for portable sequence-based monitoring of dengue virus in Brazil

• Autor(a) principal: Adelino, Talita Émile Ribeiro
• Data de Publicação: 2021
• Outros Autores: Giovanetti, Marta, Fonseca, Vagner, Xavier, Joilson, Abreu, Álvaro Salgado de, Nascimento, Valdinete Alves do, Demarchi, Luiz Henrique Ferraz, Oliveira, Marluce Aparecida Assunção, Silva, Vinícius Lemos da, Mello, Arabela Leal e Silva de, Cunha, Gabriel Muricy, Santos, Roselene Hans, Oliveira, Elaine Cristina de, Chamon Júnior, Jorge Antônio, Iani, Felipe Campos de Melo, Filippis, Ana Maria Bispo de, Abreu, André Luiz de, Jesus, Ronaldo de, Albuquerque, Carlos Frederico Campelo de, Rico, Jairo Mendez, Said, Rodrigo Fabiano do Carmo, Silva, Joscélio Aguiar, Moura, Noely Fabiana Oliveira de, Leite, Priscila, Frutuoso, Lívia Carla Vinhal, Haddad, Simone Kashima, Martínez, Alexander, Barreto, Fernanda Khouri, Vazquez, Cynthia Carolina, Cunha, Rivaldo Venâncio da, Araújo, Emerson Luiz Lima, Tosta, Stephane Fraga de Oliveira, Fabri, Allison de Araújo, Chalhoub, Flávia Lowen Levy, Lemos, Poliana da Silva, Nogueira, Fernanda de Bruycker, Lichs, Gislene Garcia de Castro, Zardin, Marina Castilho Souza Umaki, Segovia, Fátima María Cardozo, Gonçalves, Crhistinne Cavalheiro Maymone, Grillo, Zoraida Del Carmen Fernandez, Slavov, Svetoslav Nanev, Pereira, Luiz Augusto, Mendonça, Ana Flávia, Pereira, Felicidade Mota, Magalhães, Jurandy Júnior Ferraz de, Santos Júnior, Agenor de Castro Moreira dos, Lima, Maricélia Maia de, Nogueira, Rita Maria Ribeiro, Góes Neto, Aristóteles, Azevedo, Vasco Ariston de Carvalho, Ramalho, Dario Brock, Oliveira, Wanderson Kleber, Macario, Eduardo Marques, Medeiros, Arnaldo Correia de, Pimentel, Victor, Holmes, Edward C, Oliveira, Tulio de, Lourenço, José, Alcantara, Luiz Carlos Júnior, Cerqueira, Erenilde Marques de, Graf, Tiago, Ramalho, Walter, Navegantes, Wildo, Reis, Renato Barbosa, Duarte, Clara Guerra, Pereira, Maria Alves, Silva, Paulo Eduardo de Souza da, Souza, Raoni Almeida de, Pauvolid-Corrêa, Alex, Paiva, Anne Aline Pereira de, Fritsch, Hegger Machado, Mares-Guia, Maria Angélica, Torres, Maria Celeste, Lima, Maurício Teixeira, Sequeira, Patrícia, Marques, William de Almeida, Jesus, Jorlan Fernandes de, Naveca, Felipe Gomes, Silva, Alessandra Lima, Pinto, Anne Cybelle, Jaiswal, Arun Kumar, Lopes, Élisson Nogueira, Costa, Francielly Morais Rodrigues da, Peixoto, Gabriel Quintanilha, Soares, Gilson Carlos, Fonseca, Paula Luize Camargos, Souza, Renan Pedra de, Kato, Rodrigo Bentes, Santos, Rodrigo Profeta Silveira, Tiwari, Sandeep, Nogueira, Wylerson Guimarães, Santos, Beatriz Senra Álvares da Silva, Bueno, Bruna Lopes, Siqueira, Isadora Cristina de, Valle, Lourdes Farre, Borba, Melina Mosquera Navarro, Mazzetto, Alix Sandra, Aguiar, Francisco de Assis Araújo, Gomes, Irenio da Silva, Abrantes, Jayra Juliana Paiva Alves, Watanabe, Luiz Takao, Rego, Marta Ferreira da Silva, Nardy, Vanessa Brandão, Aguiar, Shirlei Ferreira de, Santos, Fabiana Cristina Pereira dos, Queiroz, Alice Louize Nunes, Nunes, Bruno Tardelli Diniz, Martins, Lívia Caricio, Nunes, Márcio Roberto Teixeira, Salles, Flávia Cristina da Silva, Claro, Ingra Morales, Jesus, Jaqueline Goes de, Cândido, Darlan da Silva, Fabbri, Cintia Marcela, González, Claudia, Saéz, Lisseth, Chen-Germán, María, Barrera, Jaime Lagos, Ramírez-González, José Ernesto, Campos, Josefina, Faller, Noelia Morel, Villalobos, Marta Eugenia Víquez, Kaslin, Roberto, Cisneros, Silvia Paola Salgado, Aburjaile, Flávia Figueira, Amaral, Carolina Dourado, Freire, Danielle Bandeira Costa de Sousa, Cruz, Laura Nogueira, Mattos, Daniel, Landeira, Leandro Ferreira Lopes, Menezes, Mariane Talon de, Orioli, Ieda Maria, Ferraz, Ariane Coelho, Oliveira, Daiane Teixeira de, Reis, Alexandre Barbosa, Cota, Renata Guerra de Sá, Bezerra, Rafael dos Santos, Falcão, Melissa Barreto, Carvalho, Rodrigo Dias de Oliveira
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
• Texto Completo: https://patua.iec.gov.br/handle/iec/4299
• topic: Dengue / virologia; Dengue / transmissão; Variação Estrutural do Genoma; Sequenciamento por Nanoporos; Vírus da Dengue / patogenicidade

Brazil experienced a large dengue virus (DENV) epidemic in 2019, highlighting a continuous struggle with effective control and public health preparedness. Using Oxford Nanopore sequencing, we led field and classroom initiatives for the monitoring of DENV in Brazil, generating 227 novel genome sequences of DENV1-2 from 85 municipalities (2015–2019). This equated to an over 50% increase in the number of DENV genomes from Brazil available in public databases. Using both phylogenetic and epidemiological models we retrospectively reconstructed the recent transmission history of DENV1-2. Phylogenetic analysis revealed complex patterns of transmission, with both lineage co-circulation and replacement. We identified two lineages within the DENV2 BR-4 clade, for which we estimated the effective reproduction number and pattern of seasonality. Overall, the surveillance outputs and training initiative described here serve as a proof-of-concept for the utility of real-time portable sequencing for research and local capacity building in the genomic surveillance of emerging viruses.