Identification of two sublineages of genotype G2 rotavirus among diarrheic children in Parauapebas, Southern Pará State, Brazil

Detalhes bibliográficos
Autor(a) principal: Mascarenhas, Joana D'Arc Pereira
Data de Publicação: 2010
Outros Autores: Lima, Clarissa Silva, Oliveira, Darleise de Souza, Guerra, Sylvia de Fátima dos Santos, Maestri, Régis Piloni, Gabbay, Yvone Benchimol, Lima, Ian Carlos Gomes de, Menezes, Euzeni Maria Costa de, Linhares, Alexandre da Costa, Bensabath, Gilberta
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
Texto Completo: https://patua.iec.gov.br/handle/iec/936
Resumo: On a world scale, group A human rotaviruses are the most common cause of severe acute gastroenteritis during infancy and childhood, including five (G1, G2, G3, G4, and G9) epidemiologically important genotypes. Among these, G2 denotes a different genogroup which appears to have a cyclic pattern of occurrence and yet little information is available about its genetic variability. The aim of this report was to characterize the emergence of G2 genotype in Paraupebas, Southern Pará State, Brazil, some of which detected after introduction of rotavirus vaccine. A total of 241 fecal specimens from young children with acute gastroenteritis were collected from the "Yutaka Takeda Hospital," a Municipality Hospital, and at the Parauapebas' Health Unit, Pará, from January to September 2006 and during March to November 2008. All samples were tested for rotavirus using immunochromatography, polyacrylamide gel electrophoresis (PAGE), and RT-PCR, yielding an overall positivity of 12.45 per cent (30/241). Rotavirus G2P[4] was identified in 27 of 30 samples (90 per cent), followed by G1P[8] (2/30, 6.67 per cent) and G9P[8] (1/30, 3.33 per cent). Phylogenetic analysis was performed in 15 of the G2 strains, all of which grouped into lineage II. Four of these strains clustered into sublineage II-a (year 2006) and 11 into one possible new sublineage named II-c (year 2008, except SAL-1920-C). The recent re-emergence of G2 genotype associated with lineage II in Brazil warrants the continuous monitoring of circulating rotavirus strains following the nationwide universal use of rotavirus vaccine.
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spelling Mascarenhas, Joana D'Arc PereiraLima, Clarissa SilvaOliveira, Darleise de SouzaGuerra, Sylvia de Fátima dos SantosMaestri, Régis PiloniGabbay, Yvone BenchimolLima, Ian Carlos Gomes deMenezes, Euzeni Maria Costa deLinhares, Alexandre da CostaBensabath, Gilberta2016-01-26T11:39:49Z2016-01-26T11:39:49Z2010MASCARENHAS, Joana D'Arc Pereira et al. Identification of two sublineages of genotype G2 rotavirus among diarrheic children in Parauapebas, Southern Pará State, Brazil. Journal of Medical Virology, v. 82, n. 4, p. 712-719, 20100146-6615https://patua.iec.gov.br/handle/iec/93610.1002/jmv.21735On a world scale, group A human rotaviruses are the most common cause of severe acute gastroenteritis during infancy and childhood, including five (G1, G2, G3, G4, and G9) epidemiologically important genotypes. Among these, G2 denotes a different genogroup which appears to have a cyclic pattern of occurrence and yet little information is available about its genetic variability. The aim of this report was to characterize the emergence of G2 genotype in Paraupebas, Southern Pará State, Brazil, some of which detected after introduction of rotavirus vaccine. A total of 241 fecal specimens from young children with acute gastroenteritis were collected from the "Yutaka Takeda Hospital," a Municipality Hospital, and at the Parauapebas' Health Unit, Pará, from January to September 2006 and during March to November 2008. All samples were tested for rotavirus using immunochromatography, polyacrylamide gel electrophoresis (PAGE), and RT-PCR, yielding an overall positivity of 12.45 per cent (30/241). Rotavirus G2P[4] was identified in 27 of 30 samples (90 per cent), followed by G1P[8] (2/30, 6.67 per cent) and G9P[8] (1/30, 3.33 per cent). Phylogenetic analysis was performed in 15 of the G2 strains, all of which grouped into lineage II. Four of these strains clustered into sublineage II-a (year 2006) and 11 into one possible new sublineage named II-c (year 2008, except SAL-1920-C). The recent re-emergence of G2 genotype associated with lineage II in Brazil warrants the continuous monitoring of circulating rotavirus strains following the nationwide universal use of rotavirus vaccine.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas, Belém, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas, Belém, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas, Belém, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas, Belém, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas, Belém, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas, Belém, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas, Belém, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas, Belém, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas, Belém, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. 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dc.title.pt_BR.fl_str_mv Identification of two sublineages of genotype G2 rotavirus among diarrheic children in Parauapebas, Southern Pará State, Brazil
title Identification of two sublineages of genotype G2 rotavirus among diarrheic children in Parauapebas, Southern Pará State, Brazil
spellingShingle Identification of two sublineages of genotype G2 rotavirus among diarrheic children in Parauapebas, Southern Pará State, Brazil
Mascarenhas, Joana D'Arc Pereira
Rotavirus
Infecções por Rotavirus / transmissão
Infecções por Rotavirus / imunologia
Diarréia
Gastroenterite
title_short Identification of two sublineages of genotype G2 rotavirus among diarrheic children in Parauapebas, Southern Pará State, Brazil
title_full Identification of two sublineages of genotype G2 rotavirus among diarrheic children in Parauapebas, Southern Pará State, Brazil
title_fullStr Identification of two sublineages of genotype G2 rotavirus among diarrheic children in Parauapebas, Southern Pará State, Brazil
title_full_unstemmed Identification of two sublineages of genotype G2 rotavirus among diarrheic children in Parauapebas, Southern Pará State, Brazil
title_sort Identification of two sublineages of genotype G2 rotavirus among diarrheic children in Parauapebas, Southern Pará State, Brazil
author Mascarenhas, Joana D'Arc Pereira
author_facet Mascarenhas, Joana D'Arc Pereira
Lima, Clarissa Silva
Oliveira, Darleise de Souza
Guerra, Sylvia de Fátima dos Santos
Maestri, Régis Piloni
Gabbay, Yvone Benchimol
Lima, Ian Carlos Gomes de
Menezes, Euzeni Maria Costa de
Linhares, Alexandre da Costa
Bensabath, Gilberta
author_role author
author2 Lima, Clarissa Silva
Oliveira, Darleise de Souza
Guerra, Sylvia de Fátima dos Santos
Maestri, Régis Piloni
Gabbay, Yvone Benchimol
Lima, Ian Carlos Gomes de
Menezes, Euzeni Maria Costa de
Linhares, Alexandre da Costa
Bensabath, Gilberta
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Mascarenhas, Joana D'Arc Pereira
Lima, Clarissa Silva
Oliveira, Darleise de Souza
Guerra, Sylvia de Fátima dos Santos
Maestri, Régis Piloni
Gabbay, Yvone Benchimol
Lima, Ian Carlos Gomes de
Menezes, Euzeni Maria Costa de
Linhares, Alexandre da Costa
Bensabath, Gilberta
dc.subject.decsPrimary.pt_BR.fl_str_mv Rotavirus
Infecções por Rotavirus / transmissão
Infecções por Rotavirus / imunologia
Diarréia
Gastroenterite
topic Rotavirus
Infecções por Rotavirus / transmissão
Infecções por Rotavirus / imunologia
Diarréia
Gastroenterite
description On a world scale, group A human rotaviruses are the most common cause of severe acute gastroenteritis during infancy and childhood, including five (G1, G2, G3, G4, and G9) epidemiologically important genotypes. Among these, G2 denotes a different genogroup which appears to have a cyclic pattern of occurrence and yet little information is available about its genetic variability. The aim of this report was to characterize the emergence of G2 genotype in Paraupebas, Southern Pará State, Brazil, some of which detected after introduction of rotavirus vaccine. A total of 241 fecal specimens from young children with acute gastroenteritis were collected from the "Yutaka Takeda Hospital," a Municipality Hospital, and at the Parauapebas' Health Unit, Pará, from January to September 2006 and during March to November 2008. All samples were tested for rotavirus using immunochromatography, polyacrylamide gel electrophoresis (PAGE), and RT-PCR, yielding an overall positivity of 12.45 per cent (30/241). Rotavirus G2P[4] was identified in 27 of 30 samples (90 per cent), followed by G1P[8] (2/30, 6.67 per cent) and G9P[8] (1/30, 3.33 per cent). Phylogenetic analysis was performed in 15 of the G2 strains, all of which grouped into lineage II. Four of these strains clustered into sublineage II-a (year 2006) and 11 into one possible new sublineage named II-c (year 2008, except SAL-1920-C). The recent re-emergence of G2 genotype associated with lineage II in Brazil warrants the continuous monitoring of circulating rotavirus strains following the nationwide universal use of rotavirus vaccine.
publishDate 2010
dc.date.issued.fl_str_mv 2010
dc.date.accessioned.fl_str_mv 2016-01-26T11:39:49Z
dc.date.available.fl_str_mv 2016-01-26T11:39:49Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv MASCARENHAS, Joana D'Arc Pereira et al. Identification of two sublineages of genotype G2 rotavirus among diarrheic children in Parauapebas, Southern Pará State, Brazil. Journal of Medical Virology, v. 82, n. 4, p. 712-719, 2010
dc.identifier.uri.fl_str_mv https://patua.iec.gov.br/handle/iec/936
dc.identifier.issn.-.fl_str_mv 0146-6615
dc.identifier.doi.-.fl_str_mv 10.1002/jmv.21735
identifier_str_mv MASCARENHAS, Joana D'Arc Pereira et al. Identification of two sublineages of genotype G2 rotavirus among diarrheic children in Parauapebas, Southern Pará State, Brazil. Journal of Medical Virology, v. 82, n. 4, p. 712-719, 2010
0146-6615
10.1002/jmv.21735
url https://patua.iec.gov.br/handle/iec/936
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