Identification of two sublineages of genotype G2 rotavirus among diarrheic children in Parauapebas, Southern Pará State, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/936 |
Resumo: | On a world scale, group A human rotaviruses are the most common cause of severe acute gastroenteritis during infancy and childhood, including five (G1, G2, G3, G4, and G9) epidemiologically important genotypes. Among these, G2 denotes a different genogroup which appears to have a cyclic pattern of occurrence and yet little information is available about its genetic variability. The aim of this report was to characterize the emergence of G2 genotype in Paraupebas, Southern Pará State, Brazil, some of which detected after introduction of rotavirus vaccine. A total of 241 fecal specimens from young children with acute gastroenteritis were collected from the "Yutaka Takeda Hospital," a Municipality Hospital, and at the Parauapebas' Health Unit, Pará, from January to September 2006 and during March to November 2008. All samples were tested for rotavirus using immunochromatography, polyacrylamide gel electrophoresis (PAGE), and RT-PCR, yielding an overall positivity of 12.45 per cent (30/241). Rotavirus G2P[4] was identified in 27 of 30 samples (90 per cent), followed by G1P[8] (2/30, 6.67 per cent) and G9P[8] (1/30, 3.33 per cent). Phylogenetic analysis was performed in 15 of the G2 strains, all of which grouped into lineage II. Four of these strains clustered into sublineage II-a (year 2006) and 11 into one possible new sublineage named II-c (year 2008, except SAL-1920-C). The recent re-emergence of G2 genotype associated with lineage II in Brazil warrants the continuous monitoring of circulating rotavirus strains following the nationwide universal use of rotavirus vaccine. |
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Mascarenhas, Joana D'Arc PereiraLima, Clarissa SilvaOliveira, Darleise de SouzaGuerra, Sylvia de Fátima dos SantosMaestri, Régis PiloniGabbay, Yvone BenchimolLima, Ian Carlos Gomes deMenezes, Euzeni Maria Costa deLinhares, Alexandre da CostaBensabath, Gilberta2016-01-26T11:39:49Z2016-01-26T11:39:49Z2010MASCARENHAS, Joana D'Arc Pereira et al. Identification of two sublineages of genotype G2 rotavirus among diarrheic children in Parauapebas, Southern Pará State, Brazil. Journal of Medical Virology, v. 82, n. 4, p. 712-719, 20100146-6615https://patua.iec.gov.br/handle/iec/93610.1002/jmv.21735On a world scale, group A human rotaviruses are the most common cause of severe acute gastroenteritis during infancy and childhood, including five (G1, G2, G3, G4, and G9) epidemiologically important genotypes. Among these, G2 denotes a different genogroup which appears to have a cyclic pattern of occurrence and yet little information is available about its genetic variability. The aim of this report was to characterize the emergence of G2 genotype in Paraupebas, Southern Pará State, Brazil, some of which detected after introduction of rotavirus vaccine. A total of 241 fecal specimens from young children with acute gastroenteritis were collected from the "Yutaka Takeda Hospital," a Municipality Hospital, and at the Parauapebas' Health Unit, Pará, from January to September 2006 and during March to November 2008. All samples were tested for rotavirus using immunochromatography, polyacrylamide gel electrophoresis (PAGE), and RT-PCR, yielding an overall positivity of 12.45 per cent (30/241). Rotavirus G2P[4] was identified in 27 of 30 samples (90 per cent), followed by G1P[8] (2/30, 6.67 per cent) and G9P[8] (1/30, 3.33 per cent). Phylogenetic analysis was performed in 15 of the G2 strains, all of which grouped into lineage II. Four of these strains clustered into sublineage II-a (year 2006) and 11 into one possible new sublineage named II-c (year 2008, except SAL-1920-C). The recent re-emergence of G2 genotype associated with lineage II in Brazil warrants the continuous monitoring of circulating rotavirus strains following the nationwide universal use of rotavirus vaccine.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas, Belém, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas, Belém, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas, Belém, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas, Belém, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas, Belém, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas, Belém, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas, Belém, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas, Belém, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas, Belém, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas, Belém, PA, Brasil.application/pdfengWileyIdentification of two sublineages of genotype G2 rotavirus among diarrheic children in Parauapebas, Southern Pará State, Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleRotavirusInfecções por Rotavirus / transmissãoInfecções por Rotavirus / imunologiaDiarréiaGastroenteriteinfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALIdentification of two sublineages of genotype G2 rotavirus among diarrheic children in Parauapebas, Southern Pará State, Brazil .pdfapplication/pdf355834https://patua.iec.gov.br/bitstreams/512f4cd1-1eaf-4117-b9c0-e0d05dbc2f38/downloadefb50aad8bb37bde656e3b602f337ff0MD51TEXTfile_1.pdf.txtfile_1.pdf.txtExtracted texttext/plain31318https://patua.iec.gov.br/bitstreams/f82ee229-1669-4bed-b9b4-c9290b11dfc8/downloadfeef57c306aa7c88032b12aa706a7ef6MD52Identification of two sublineages of genotype G2 rotavirus among diarrheic children in Parauapebas, Southern Pará State, Brazil .pdf.txtIdentification of two sublineages of genotype G2 rotavirus among diarrheic children in Parauapebas, Southern Pará State, Brazil .pdf.txtExtracted texttext/plain31467https://patua.iec.gov.br/bitstreams/6eaddb7c-112c-44db-9fcf-31ef3facad37/download8c4d8e50ce8bace5bff934e975142c10MD56THUMBNAILIdentification of two sublineages of genotype G2 rotavirus among diarrheic children in Parauapebas, Southern Pará State, Brazil .pdf.jpgIdentification of two sublineages of genotype G2 rotavirus among diarrheic children in Parauapebas, Southern Pará State, Brazil .pdf.jpgGenerated Thumbnailimage/jpeg6319https://patua.iec.gov.br/bitstreams/4a1052b2-799b-466c-a01e-2fa3512f484d/downloadbb34336d35f320326d664af6df88bab4MD57LICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv |
Identification of two sublineages of genotype G2 rotavirus among diarrheic children in Parauapebas, Southern Pará State, Brazil |
title |
Identification of two sublineages of genotype G2 rotavirus among diarrheic children in Parauapebas, Southern Pará State, Brazil |
spellingShingle |
Identification of two sublineages of genotype G2 rotavirus among diarrheic children in Parauapebas, Southern Pará State, Brazil Mascarenhas, Joana D'Arc Pereira Rotavirus Infecções por Rotavirus / transmissão Infecções por Rotavirus / imunologia Diarréia Gastroenterite |
title_short |
Identification of two sublineages of genotype G2 rotavirus among diarrheic children in Parauapebas, Southern Pará State, Brazil |
title_full |
Identification of two sublineages of genotype G2 rotavirus among diarrheic children in Parauapebas, Southern Pará State, Brazil |
title_fullStr |
Identification of two sublineages of genotype G2 rotavirus among diarrheic children in Parauapebas, Southern Pará State, Brazil |
title_full_unstemmed |
Identification of two sublineages of genotype G2 rotavirus among diarrheic children in Parauapebas, Southern Pará State, Brazil |
title_sort |
Identification of two sublineages of genotype G2 rotavirus among diarrheic children in Parauapebas, Southern Pará State, Brazil |
author |
Mascarenhas, Joana D'Arc Pereira |
author_facet |
Mascarenhas, Joana D'Arc Pereira Lima, Clarissa Silva Oliveira, Darleise de Souza Guerra, Sylvia de Fátima dos Santos Maestri, Régis Piloni Gabbay, Yvone Benchimol Lima, Ian Carlos Gomes de Menezes, Euzeni Maria Costa de Linhares, Alexandre da Costa Bensabath, Gilberta |
author_role |
author |
author2 |
Lima, Clarissa Silva Oliveira, Darleise de Souza Guerra, Sylvia de Fátima dos Santos Maestri, Régis Piloni Gabbay, Yvone Benchimol Lima, Ian Carlos Gomes de Menezes, Euzeni Maria Costa de Linhares, Alexandre da Costa Bensabath, Gilberta |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Mascarenhas, Joana D'Arc Pereira Lima, Clarissa Silva Oliveira, Darleise de Souza Guerra, Sylvia de Fátima dos Santos Maestri, Régis Piloni Gabbay, Yvone Benchimol Lima, Ian Carlos Gomes de Menezes, Euzeni Maria Costa de Linhares, Alexandre da Costa Bensabath, Gilberta |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Rotavirus Infecções por Rotavirus / transmissão Infecções por Rotavirus / imunologia Diarréia Gastroenterite |
topic |
Rotavirus Infecções por Rotavirus / transmissão Infecções por Rotavirus / imunologia Diarréia Gastroenterite |
description |
On a world scale, group A human rotaviruses are the most common cause of severe acute gastroenteritis during infancy and childhood, including five (G1, G2, G3, G4, and G9) epidemiologically important genotypes. Among these, G2 denotes a different genogroup which appears to have a cyclic pattern of occurrence and yet little information is available about its genetic variability. The aim of this report was to characterize the emergence of G2 genotype in Paraupebas, Southern Pará State, Brazil, some of which detected after introduction of rotavirus vaccine. A total of 241 fecal specimens from young children with acute gastroenteritis were collected from the "Yutaka Takeda Hospital," a Municipality Hospital, and at the Parauapebas' Health Unit, Pará, from January to September 2006 and during March to November 2008. All samples were tested for rotavirus using immunochromatography, polyacrylamide gel electrophoresis (PAGE), and RT-PCR, yielding an overall positivity of 12.45 per cent (30/241). Rotavirus G2P[4] was identified in 27 of 30 samples (90 per cent), followed by G1P[8] (2/30, 6.67 per cent) and G9P[8] (1/30, 3.33 per cent). Phylogenetic analysis was performed in 15 of the G2 strains, all of which grouped into lineage II. Four of these strains clustered into sublineage II-a (year 2006) and 11 into one possible new sublineage named II-c (year 2008, except SAL-1920-C). The recent re-emergence of G2 genotype associated with lineage II in Brazil warrants the continuous monitoring of circulating rotavirus strains following the nationwide universal use of rotavirus vaccine. |
publishDate |
2010 |
dc.date.issued.fl_str_mv |
2010 |
dc.date.accessioned.fl_str_mv |
2016-01-26T11:39:49Z |
dc.date.available.fl_str_mv |
2016-01-26T11:39:49Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MASCARENHAS, Joana D'Arc Pereira et al. Identification of two sublineages of genotype G2 rotavirus among diarrheic children in Parauapebas, Southern Pará State, Brazil. Journal of Medical Virology, v. 82, n. 4, p. 712-719, 2010 |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/936 |
dc.identifier.issn.-.fl_str_mv |
0146-6615 |
dc.identifier.doi.-.fl_str_mv |
10.1002/jmv.21735 |
identifier_str_mv |
MASCARENHAS, Joana D'Arc Pereira et al. Identification of two sublineages of genotype G2 rotavirus among diarrheic children in Parauapebas, Southern Pará State, Brazil. Journal of Medical Virology, v. 82, n. 4, p. 712-719, 2010 0146-6615 10.1002/jmv.21735 |
url |
https://patua.iec.gov.br/handle/iec/936 |
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eng |
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eng |
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openAccess |
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Wiley |
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