Arboviruses in free-ranging birds and hematophagous arthropods (Diptera, Nematocera) from forest remnants and urbanized areas of an environmental protection area in the amazon biome

Detalhes bibliográficos
Autor(a) principal: Ramos, Bruna Alves
Data de Publicação: 2022
Outros Autores: Chagas, Liliane Leal das, Silva, Franko de Arruda e, Santos, Eder Barros dos, Chiang, Jannifer Oliveira, Nunes Neto, Joaquim Pinto, Vieira, Durval Bertram Rodrigues, Rosa Junior, José Wilson, Silva, Eliana Vieira Pinto da, Freitas, Maria de Nazaré Oliveira, Santos, Maissa Maia, Pantoja, Jamilla Augusta de Sousa, Gonçalves, Ercília de Jesus, Barros, Landeson Junior Leopoldino, Silva, Sandro Patroca da, Aragão, Carine Fortes, Cruz, Ana Cecília Ribeiro, Casseb, Lívia Medeiros Neves, Souto, Lizandra Caroline dos Santos, Mascarenhas, Joana D'Arc Pereira, Furtado, Erilene Cristina da Silva, Silva, Raimundo Nelson Souza da, Casseb, Alexandre do Rosário, Martins, Lívia Caricio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
Texto Completo: https://patua.iec.gov.br/handle/iec/4708
Resumo: The rapid and disorderly urbanization in the Amazon has resulted in the insertion of forest fragments into cities, causing the circulation of arboviruses, which can involve hematophagous arthropods and free-ranging birds in the transmission cycles in urban environments. This study aimed to evaluate the circulation of arboviruses in free ranging birds and hematophagous arthropods captured in an Environmental Protection Area in the Belem metropolitan area, Brazil. Birds were captured using mist nets, and hematophagous arthropods were collected using a human protected attraction technique and light traps. The birds’ sera were subjected to a hemagglutination inhibition test to detect antibodies against 29 arbovirus antigens. Arthropod macerates were inoculated into C6/36 and VERO cell cultures to attempt viral isolation and were tested using indirect immunofluorescence, subsequent genetic sequencing and submitted for phylogenetic analysis. Four bird sera were positive for arbovirus, and one batch of Psorophora ferox was positive for Flavivirus on viral isolation and indirect immunofluorescence. In addition, the Ilheus virus was detected in the sequencing and phylogenetic analysis. The presence of antibodies in sera from free-ranging birds and the isolation of Ilheus virus in Psorophora ferox indicate the circulation of arboviruses in forest remnants in the urban center of Belem.
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spelling Ramos, Bruna AlvesChagas, Liliane Leal dasSilva, Franko de Arruda eSantos, Eder Barros dosChiang, Jannifer OliveiraNunes Neto, Joaquim PintoVieira, Durval Bertram RodriguesRosa Junior, José WilsonSilva, Eliana Vieira Pinto daFreitas, Maria de Nazaré OliveiraSantos, Maissa MaiaPantoja, Jamilla Augusta de SousaGonçalves, Ercília de JesusBarros, Landeson Junior LeopoldinoSilva, Sandro Patroca daAragão, Carine FortesCruz, Ana Cecília RibeiroCasseb, Lívia Medeiros NevesSouto, Lizandra Caroline dos SantosMascarenhas, Joana D'Arc PereiraFurtado, Erilene Cristina da SilvaSilva, Raimundo Nelson Souza daCasseb, Alexandre do RosárioMartins, Lívia Caricio2022-11-07T13:58:50Z2022-11-07T13:58:50Z2022RAMOS, Bruna Alves et al. Arboviruses in free-ranging birds and hematophagous arthropods (Diptera, Nematocera) from forest remnants and urbanized areas of an environmental protection area in the amazon biome. Viruses, v. 14, n. 10, p. 1-14, Oct. 2022. DOI: https://doi.org/10.3390/v14102101. Disponível em: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9608863/pdf/viruses-14-02101.pdf.1999-4915https://patua.iec.gov.br/handle/iec/470810.3390/v14102101The rapid and disorderly urbanization in the Amazon has resulted in the insertion of forest fragments into cities, causing the circulation of arboviruses, which can involve hematophagous arthropods and free-ranging birds in the transmission cycles in urban environments. This study aimed to evaluate the circulation of arboviruses in free ranging birds and hematophagous arthropods captured in an Environmental Protection Area in the Belem metropolitan area, Brazil. Birds were captured using mist nets, and hematophagous arthropods were collected using a human protected attraction technique and light traps. The birds’ sera were subjected to a hemagglutination inhibition test to detect antibodies against 29 arbovirus antigens. Arthropod macerates were inoculated into C6/36 and VERO cell cultures to attempt viral isolation and were tested using indirect immunofluorescence, subsequent genetic sequencing and submitted for phylogenetic analysis. Four bird sera were positive for arbovirus, and one batch of Psorophora ferox was positive for Flavivirus on viral isolation and indirect immunofluorescence. In addition, the Ilheus virus was detected in the sequencing and phylogenetic analysis. The presence of antibodies in sera from free-ranging birds and the isolation of Ilheus virus in Psorophora ferox indicate the circulation of arboviruses in forest remnants in the urban center of Belem.This research was financed using resources provided in the form of scholarships by the National Council for Scientific and Technological Development (CNPq—process number 141723/2018- 1), and using resources made available by the Higher Education Personnel Improvement Coordination (CAPES), through the Graduate Support Program (PROAP).Ministério da Saúde. Secretaria de Ciência, Tecnologia, Inovação e Insumos Estratégicos. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Ciência, Tecnologia, Inovação e Insumos Estratégicos. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Ciência, Tecnologia, Inovação e Insumos Estratégicos. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Ciência, Tecnologia, Inovação e Insumos Estratégicos. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Ciência, Tecnologia, Inovação e Insumos Estratégicos. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Ciência, Tecnologia, Inovação e Insumos Estratégicos. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Ciência, Tecnologia, Inovação e Insumos Estratégicos. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Ciência, Tecnologia, Inovação e Insumos Estratégicos. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Ciência, Tecnologia, Inovação e Insumos Estratégicos. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Ciência, Tecnologia, Inovação e Insumos Estratégicos. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Ciência, Tecnologia, Inovação e Insumos Estratégicos. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Ciência, Tecnologia, Inovação e Insumos Estratégicos. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Ciência, Tecnologia, Inovação e Insumos Estratégicos. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Ciência, Tecnologia, Inovação e Insumos Estratégicos. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Ciência, Tecnologia, Inovação e Insumos Estratégicos. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Ciência, Tecnologia, Inovação e Insumos Estratégicos. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Ciência, Tecnologia, Inovação e Insumos Estratégicos. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Ciência, Tecnologia, Inovação e Insumos Estratégicos. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Ciência, Tecnologia, Inovação e Insumos Estratégicos. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Central Laboratory of Pará State. Belem, PA, Brazil.Federal Rural University of Amazonia. Health and Animal Production Institute. Belem, PA, Brazil.Federal Rural University of Amazonia. Health and Animal Production Institute. Belem, PA, Brazil.Ministério da Saúde. Secretaria de Ciência, Tecnologia, Inovação e Insumos Estratégicos. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Ciência, Tecnologia, Inovação e Insumos Estratégicos. Instituto Evandro Chagas. 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dc.title.pt_BR.fl_str_mv Arboviruses in free-ranging birds and hematophagous arthropods (Diptera, Nematocera) from forest remnants and urbanized areas of an environmental protection area in the amazon biome
title Arboviruses in free-ranging birds and hematophagous arthropods (Diptera, Nematocera) from forest remnants and urbanized areas of an environmental protection area in the amazon biome
spellingShingle Arboviruses in free-ranging birds and hematophagous arthropods (Diptera, Nematocera) from forest remnants and urbanized areas of an environmental protection area in the amazon biome
Ramos, Bruna Alves
Arbovírus / isolamento & purificação
Infecções por Arbovirus / transmissão
Conservação dos Recursos Naturais
Urbanização
Aves
Artrópodes
title_short Arboviruses in free-ranging birds and hematophagous arthropods (Diptera, Nematocera) from forest remnants and urbanized areas of an environmental protection area in the amazon biome
title_full Arboviruses in free-ranging birds and hematophagous arthropods (Diptera, Nematocera) from forest remnants and urbanized areas of an environmental protection area in the amazon biome
title_fullStr Arboviruses in free-ranging birds and hematophagous arthropods (Diptera, Nematocera) from forest remnants and urbanized areas of an environmental protection area in the amazon biome
title_full_unstemmed Arboviruses in free-ranging birds and hematophagous arthropods (Diptera, Nematocera) from forest remnants and urbanized areas of an environmental protection area in the amazon biome
title_sort Arboviruses in free-ranging birds and hematophagous arthropods (Diptera, Nematocera) from forest remnants and urbanized areas of an environmental protection area in the amazon biome
author Ramos, Bruna Alves
author_facet Ramos, Bruna Alves
Chagas, Liliane Leal das
Silva, Franko de Arruda e
Santos, Eder Barros dos
Chiang, Jannifer Oliveira
Nunes Neto, Joaquim Pinto
Vieira, Durval Bertram Rodrigues
Rosa Junior, José Wilson
Silva, Eliana Vieira Pinto da
Freitas, Maria de Nazaré Oliveira
Santos, Maissa Maia
Pantoja, Jamilla Augusta de Sousa
Gonçalves, Ercília de Jesus
Barros, Landeson Junior Leopoldino
Silva, Sandro Patroca da
Aragão, Carine Fortes
Cruz, Ana Cecília Ribeiro
Casseb, Lívia Medeiros Neves
Souto, Lizandra Caroline dos Santos
Mascarenhas, Joana D'Arc Pereira
Furtado, Erilene Cristina da Silva
Silva, Raimundo Nelson Souza da
Casseb, Alexandre do Rosário
Martins, Lívia Caricio
author_role author
author2 Chagas, Liliane Leal das
Silva, Franko de Arruda e
Santos, Eder Barros dos
Chiang, Jannifer Oliveira
Nunes Neto, Joaquim Pinto
Vieira, Durval Bertram Rodrigues
Rosa Junior, José Wilson
Silva, Eliana Vieira Pinto da
Freitas, Maria de Nazaré Oliveira
Santos, Maissa Maia
Pantoja, Jamilla Augusta de Sousa
Gonçalves, Ercília de Jesus
Barros, Landeson Junior Leopoldino
Silva, Sandro Patroca da
Aragão, Carine Fortes
Cruz, Ana Cecília Ribeiro
Casseb, Lívia Medeiros Neves
Souto, Lizandra Caroline dos Santos
Mascarenhas, Joana D'Arc Pereira
Furtado, Erilene Cristina da Silva
Silva, Raimundo Nelson Souza da
Casseb, Alexandre do Rosário
Martins, Lívia Caricio
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Ramos, Bruna Alves
Chagas, Liliane Leal das
Silva, Franko de Arruda e
Santos, Eder Barros dos
Chiang, Jannifer Oliveira
Nunes Neto, Joaquim Pinto
Vieira, Durval Bertram Rodrigues
Rosa Junior, José Wilson
Silva, Eliana Vieira Pinto da
Freitas, Maria de Nazaré Oliveira
Santos, Maissa Maia
Pantoja, Jamilla Augusta de Sousa
Gonçalves, Ercília de Jesus
Barros, Landeson Junior Leopoldino
Silva, Sandro Patroca da
Aragão, Carine Fortes
Cruz, Ana Cecília Ribeiro
Casseb, Lívia Medeiros Neves
Souto, Lizandra Caroline dos Santos
Mascarenhas, Joana D'Arc Pereira
Furtado, Erilene Cristina da Silva
Silva, Raimundo Nelson Souza da
Casseb, Alexandre do Rosário
Martins, Lívia Caricio
dc.subject.decsPrimary.pt_BR.fl_str_mv Arbovírus / isolamento & purificação
Infecções por Arbovirus / transmissão
Conservação dos Recursos Naturais
Urbanização
Aves
Artrópodes
topic Arbovírus / isolamento & purificação
Infecções por Arbovirus / transmissão
Conservação dos Recursos Naturais
Urbanização
Aves
Artrópodes
description The rapid and disorderly urbanization in the Amazon has resulted in the insertion of forest fragments into cities, causing the circulation of arboviruses, which can involve hematophagous arthropods and free-ranging birds in the transmission cycles in urban environments. This study aimed to evaluate the circulation of arboviruses in free ranging birds and hematophagous arthropods captured in an Environmental Protection Area in the Belem metropolitan area, Brazil. Birds were captured using mist nets, and hematophagous arthropods were collected using a human protected attraction technique and light traps. The birds’ sera were subjected to a hemagglutination inhibition test to detect antibodies against 29 arbovirus antigens. Arthropod macerates were inoculated into C6/36 and VERO cell cultures to attempt viral isolation and were tested using indirect immunofluorescence, subsequent genetic sequencing and submitted for phylogenetic analysis. Four bird sera were positive for arbovirus, and one batch of Psorophora ferox was positive for Flavivirus on viral isolation and indirect immunofluorescence. In addition, the Ilheus virus was detected in the sequencing and phylogenetic analysis. The presence of antibodies in sera from free-ranging birds and the isolation of Ilheus virus in Psorophora ferox indicate the circulation of arboviruses in forest remnants in the urban center of Belem.
publishDate 2022
dc.date.accessioned.fl_str_mv 2022-11-07T13:58:50Z
dc.date.available.fl_str_mv 2022-11-07T13:58:50Z
dc.date.issued.fl_str_mv 2022
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv RAMOS, Bruna Alves et al. Arboviruses in free-ranging birds and hematophagous arthropods (Diptera, Nematocera) from forest remnants and urbanized areas of an environmental protection area in the amazon biome. Viruses, v. 14, n. 10, p. 1-14, Oct. 2022. DOI: https://doi.org/10.3390/v14102101. Disponível em: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9608863/pdf/viruses-14-02101.pdf.
dc.identifier.uri.fl_str_mv https://patua.iec.gov.br/handle/iec/4708
dc.identifier.issn.-.fl_str_mv 1999-4915
dc.identifier.doi.pt_BR.fl_str_mv 10.3390/v14102101
identifier_str_mv RAMOS, Bruna Alves et al. Arboviruses in free-ranging birds and hematophagous arthropods (Diptera, Nematocera) from forest remnants and urbanized areas of an environmental protection area in the amazon biome. Viruses, v. 14, n. 10, p. 1-14, Oct. 2022. DOI: https://doi.org/10.3390/v14102101. Disponível em: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9608863/pdf/viruses-14-02101.pdf.
1999-4915
10.3390/v14102101
url https://patua.iec.gov.br/handle/iec/4708
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