Surveillance of cutaneous leishmaniasis in clinical samples: distribution of Leishmania guyanensis in the state of Amapá, Brazil, 2018

• Autor(a) principal: Almeida, Ariely Nunes Ferreira de
• Data de Publicação: 2020
• Outros Autores: Nascimento, Luciana de Cássia Silva do, Sousa, Edith Silvia Moura de Moura, Oliveira, Afonso José Diger de, Sena, Maria Gorete de, Resende, Breno Maués de, Chaves, Raimunda Cleide Gonçalves, Santos, Lourdes Maria Garcez dos
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
• Texto Completo: https://patua.iec.gov.br/handle/iec/4082
• topic: Leishmaniose Cutânea / patologia; Leishmania guyanensis / parasitologia; Epidemiologia Molecular / métodos; Ecossistema Amazônico; Amapá (AP)

Objective: to investigate Leishmania species in a series of autochthonous cutaneous leishmaniasis (CL) cases in Amapá State, Brazilian Amazon. Methods: this was a descriptive ecological study carried out from January-October/2018 at a reference center for CL diagnosis in Amapá; individuals with CL receiving care from January-May/2018 were recruited; clinical data and skin biopsies were obtained; from extracted DNA (phenol-chloroform) we amplified the hsp70-234 gene region (PCR) for nucleotide sequencing (Applied Biosystems: ABI3500XL). Results: 38 individuals were interviewed, examined and diagnosed; men predominated (28/38; mean age=32.5±11.3); lesions (most ulcers: 37/38) measuring 0,4-10mm (34/38) and ≥11mm (4/38) were multiple in 20/38 individuals; diagnosis of L. braziliensis (1), L. naiffi (1), L. infantum (1), L. (Viannia) sp. (1), L. amazonensis (2) and L. guyanensis (32); individuals infected with L. guyanensis (32/38) lived in 9/10 municipalities represented in the sample, and 17/32 of these had multiple lesions. Conclusion: presence of Leishmania guyanensis predominated and was frequently associated with multiple lesions.