Detection and genotyping of enteric viruses in hospitalized children with acute gastroenteritis in Belém, Brazil: occurrence of adenovirus viremia by species F, types 40/41
Autor(a) principal: | |
---|---|
Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/3529 |
Resumo: | Enteric adenovirus (AdV), sapovirus (SaV), and human astrovirus (HAstV) are important pathogens involved in the gastroenteritis etiology. In this study, a total of 219 fecal samples and sera were collected from children hospitalized for acute gastroenteritis (AGE) in two large pediatric hospitals in Belém, from March 2012 to April 2015. The samples were analyzed by polymerase chain reaction (PCR) for adenovirus (AdV) and HAstV (astrovirus) detection, and Nested-PCR and qPCR for sapovirus (SaV) detection. AdV was detected in 50.2% (110/219) of the cases, with 42.7% (47/110) being sequenced and classified as: species F (63.9% - 30/47), A (4.2% - 2/47), B (6.4% - 3/47), C (17.1% - 8/47), D (4.2% - 2/47), and E (4.2% - 2/47). Of the 110 AdV-positive faeces samples, 80 paired sera presented sufficient amounts and were also tested for this virus, of which 51 (63.7%) showed positive results and 26 (70.3%) pairs (feces plus sera) presented concordant results after sequencing being classified as: species F (21/26; 80.8%), A (1/26; 3.8%), B (1/26; 3.8%), and C (3/26; 11.5%). Overall, HAstV rate in the feces samples was 1.8% (4/219), including both HAstV-1a (2/4; 50%) and HAstV-2c (2/4; 50%). SaV was detected in 4.6% (10/219) of the fecal samples, out of which 50% (5/10) of the positive samples were characterized into the genogroups GI.1 (1), GI.2 (2), and GII.4 (2). These findings highlighted the important contributions of AdV, HAstV, and SaV in the enteric virus spectrum in our region and showed the high genetic diversity of AdV. In addition, it demonstrated for the first time in Brazil, the circulation of AdV in the serum of hospitalized children with AGE. |
id |
IEC-2_5069990eebff30a50bf056a11c025806 |
---|---|
oai_identifier_str |
oai:patua.iec.gov.br:iec/3529 |
network_acronym_str |
IEC-2 |
network_name_str |
Repositório Digital do Instituto Evandro Chagas (Patuá) |
repository_id_str |
|
spelling |
Portal, Thayara MoraisReymão, Tammy Kathlyn AmaralQuinderé Neto, Germano AlvesFiuza, Mercedes Kamila da CTeixeira, Dielle MonteiroLima, Ian Carlos GomesSousa Júnior, Edivaldo CostaBandeira, Renato da SilvaDeus, Danielle Rodrigues deJustino, Maria Cleonice AguiarLinhares, Alexandre da CostaSilva, Luciana Damascena daResque, Hugo ReisGabbay, Yvone Benchimol2018-11-13T19:46:19Z2018-11-13T19:46:19Z2019PORTAL, Thayara Morais et al. Detection and genotyping of enteric viruses in hospitalized children with acute gastroenteritis in Belém, Brazil: occurrence of adenovirus viremia by species F, types 40/41. Journal of Medical Virology, v. 91, n. 3, p. 378-384, Jan. 2019.0146-6615https://patua.iec.gov.br/handle/iec/352910.1002/jmv.25321Enteric adenovirus (AdV), sapovirus (SaV), and human astrovirus (HAstV) are important pathogens involved in the gastroenteritis etiology. In this study, a total of 219 fecal samples and sera were collected from children hospitalized for acute gastroenteritis (AGE) in two large pediatric hospitals in Belém, from March 2012 to April 2015. The samples were analyzed by polymerase chain reaction (PCR) for adenovirus (AdV) and HAstV (astrovirus) detection, and Nested-PCR and qPCR for sapovirus (SaV) detection. AdV was detected in 50.2% (110/219) of the cases, with 42.7% (47/110) being sequenced and classified as: species F (63.9% - 30/47), A (4.2% - 2/47), B (6.4% - 3/47), C (17.1% - 8/47), D (4.2% - 2/47), and E (4.2% - 2/47). Of the 110 AdV-positive faeces samples, 80 paired sera presented sufficient amounts and were also tested for this virus, of which 51 (63.7%) showed positive results and 26 (70.3%) pairs (feces plus sera) presented concordant results after sequencing being classified as: species F (21/26; 80.8%), A (1/26; 3.8%), B (1/26; 3.8%), and C (3/26; 11.5%). Overall, HAstV rate in the feces samples was 1.8% (4/219), including both HAstV-1a (2/4; 50%) and HAstV-2c (2/4; 50%). SaV was detected in 4.6% (10/219) of the fecal samples, out of which 50% (5/10) of the positive samples were characterized into the genogroups GI.1 (1), GI.2 (2), and GII.4 (2). These findings highlighted the important contributions of AdV, HAstV, and SaV in the enteric virus spectrum in our region and showed the high genetic diversity of AdV. In addition, it demonstrated for the first time in Brazil, the circulation of AdV in the serum of hospitalized children with AGE.This research was supported by Evandro Chagas Institute; Capes.University of State of Pará. Postgraduate Program in Parasitic Biology in the Amazon. Belém, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas.Program de Pós-Graduação em Virologia. Ananindeua, PA, Brasil.University of State of Pará. Undergraduate in Nursing. Belém, PA, Brazil.School of the Amazon. Belém, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.University of State of Pará. Postgraduate Program in Parasitic Biology in the Amazon. Belém, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.engWileyDetection and genotyping of enteric viruses in hospitalized children with acute gastroenteritis in Belém, Brazil: occurrence of adenovirus viremia by species F, types 40/41info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGastroenterite / patologiaInfecções por Adenovirus HumanosAdenovírus Humanos / patogenicidadeSapovirusMamastrovirusDiarreia Infantil / patologiaReação em Cadeia da Polimerase / métodosinfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALDetection and genotyping of enteric viruses in hospitalized children with acute gastroenteritis in Belém, Brazil: Occurrence of adenovirus viremia by species F, types 40/41.pdfDetection and genotyping of enteric viruses in hospitalized children with acute gastroenteritis in Belém, Brazil: Occurrence of adenovirus viremia by species F, types 40/41.pdfapplication/pdf842080https://patua.iec.gov.br/bitstreams/dfb042b5-44a0-48d5-b953-497db329c60e/download6a58d733bbe123f9a6f3d02aa8287a0dMD53LICENSElicense.txtlicense.txttext/plain; charset=utf-871https://patua.iec.gov.br/bitstreams/8ecb6bac-30ea-4e34-94c1-67ac30a46cba/download52f1732ea66fbd1123abe39f5373b797MD52TEXTDetection and genotyping of enteric viruses in hospitalized children with acute gastroenteritis in Belém, Brazil: Occurrence of adenovirus viremia by species F, types 40/41.pdf.txtDetection and genotyping of enteric viruses in hospitalized children with acute gastroenteritis in Belém, Brazil: Occurrence of adenovirus viremia by species F, types 40/41.pdf.txtExtracted texttext/plain37712https://patua.iec.gov.br/bitstreams/737cf747-f8cf-4156-886c-cda327854465/download81a0e12b439a341593fb50aeb89768a7MD56THUMBNAILDetection and genotyping of enteric viruses in hospitalized children with acute gastroenteritis in Belém, Brazil: Occurrence of adenovirus viremia by species F, types 40/41.pdf.jpgDetection and genotyping of enteric viruses in hospitalized children with acute gastroenteritis in Belém, Brazil: Occurrence of adenovirus viremia by species F, types 40/41.pdf.jpgGenerated Thumbnailimage/jpeg6463https://patua.iec.gov.br/bitstreams/86700d43-c694-474b-ba9a-6381b4139dd7/download09df96cf9b54b88bd62ffba9101062daMD57iec/35292022-10-20 21:44:45.98oai:patua.iec.gov.br:iec/3529https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-20T21:44:45Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)falseVG9kb3Mgb3MgZG9jdW1lbnRvcyBkZXNzYSBjb2xlw6fDo28gc2VndWVtIGEgTGljZW7Dp2EgQ3JlYXRpdmUgY29tbW9ucy4= |
dc.title.pt_BR.fl_str_mv |
Detection and genotyping of enteric viruses in hospitalized children with acute gastroenteritis in Belém, Brazil: occurrence of adenovirus viremia by species F, types 40/41 |
title |
Detection and genotyping of enteric viruses in hospitalized children with acute gastroenteritis in Belém, Brazil: occurrence of adenovirus viremia by species F, types 40/41 |
spellingShingle |
Detection and genotyping of enteric viruses in hospitalized children with acute gastroenteritis in Belém, Brazil: occurrence of adenovirus viremia by species F, types 40/41 Portal, Thayara Morais Gastroenterite / patologia Infecções por Adenovirus Humanos Adenovírus Humanos / patogenicidade Sapovirus Mamastrovirus Diarreia Infantil / patologia Reação em Cadeia da Polimerase / métodos |
title_short |
Detection and genotyping of enteric viruses in hospitalized children with acute gastroenteritis in Belém, Brazil: occurrence of adenovirus viremia by species F, types 40/41 |
title_full |
Detection and genotyping of enteric viruses in hospitalized children with acute gastroenteritis in Belém, Brazil: occurrence of adenovirus viremia by species F, types 40/41 |
title_fullStr |
Detection and genotyping of enteric viruses in hospitalized children with acute gastroenteritis in Belém, Brazil: occurrence of adenovirus viremia by species F, types 40/41 |
title_full_unstemmed |
Detection and genotyping of enteric viruses in hospitalized children with acute gastroenteritis in Belém, Brazil: occurrence of adenovirus viremia by species F, types 40/41 |
title_sort |
Detection and genotyping of enteric viruses in hospitalized children with acute gastroenteritis in Belém, Brazil: occurrence of adenovirus viremia by species F, types 40/41 |
author |
Portal, Thayara Morais |
author_facet |
Portal, Thayara Morais Reymão, Tammy Kathlyn Amaral Quinderé Neto, Germano Alves Fiuza, Mercedes Kamila da C Teixeira, Dielle Monteiro Lima, Ian Carlos Gomes Sousa Júnior, Edivaldo Costa Bandeira, Renato da Silva Deus, Danielle Rodrigues de Justino, Maria Cleonice Aguiar Linhares, Alexandre da Costa Silva, Luciana Damascena da Resque, Hugo Reis Gabbay, Yvone Benchimol |
author_role |
author |
author2 |
Reymão, Tammy Kathlyn Amaral Quinderé Neto, Germano Alves Fiuza, Mercedes Kamila da C Teixeira, Dielle Monteiro Lima, Ian Carlos Gomes Sousa Júnior, Edivaldo Costa Bandeira, Renato da Silva Deus, Danielle Rodrigues de Justino, Maria Cleonice Aguiar Linhares, Alexandre da Costa Silva, Luciana Damascena da Resque, Hugo Reis Gabbay, Yvone Benchimol |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Portal, Thayara Morais Reymão, Tammy Kathlyn Amaral Quinderé Neto, Germano Alves Fiuza, Mercedes Kamila da C Teixeira, Dielle Monteiro Lima, Ian Carlos Gomes Sousa Júnior, Edivaldo Costa Bandeira, Renato da Silva Deus, Danielle Rodrigues de Justino, Maria Cleonice Aguiar Linhares, Alexandre da Costa Silva, Luciana Damascena da Resque, Hugo Reis Gabbay, Yvone Benchimol |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Gastroenterite / patologia Infecções por Adenovirus Humanos Adenovírus Humanos / patogenicidade Sapovirus Mamastrovirus Diarreia Infantil / patologia Reação em Cadeia da Polimerase / métodos |
topic |
Gastroenterite / patologia Infecções por Adenovirus Humanos Adenovírus Humanos / patogenicidade Sapovirus Mamastrovirus Diarreia Infantil / patologia Reação em Cadeia da Polimerase / métodos |
description |
Enteric adenovirus (AdV), sapovirus (SaV), and human astrovirus (HAstV) are important pathogens involved in the gastroenteritis etiology. In this study, a total of 219 fecal samples and sera were collected from children hospitalized for acute gastroenteritis (AGE) in two large pediatric hospitals in Belém, from March 2012 to April 2015. The samples were analyzed by polymerase chain reaction (PCR) for adenovirus (AdV) and HAstV (astrovirus) detection, and Nested-PCR and qPCR for sapovirus (SaV) detection. AdV was detected in 50.2% (110/219) of the cases, with 42.7% (47/110) being sequenced and classified as: species F (63.9% - 30/47), A (4.2% - 2/47), B (6.4% - 3/47), C (17.1% - 8/47), D (4.2% - 2/47), and E (4.2% - 2/47). Of the 110 AdV-positive faeces samples, 80 paired sera presented sufficient amounts and were also tested for this virus, of which 51 (63.7%) showed positive results and 26 (70.3%) pairs (feces plus sera) presented concordant results after sequencing being classified as: species F (21/26; 80.8%), A (1/26; 3.8%), B (1/26; 3.8%), and C (3/26; 11.5%). Overall, HAstV rate in the feces samples was 1.8% (4/219), including both HAstV-1a (2/4; 50%) and HAstV-2c (2/4; 50%). SaV was detected in 4.6% (10/219) of the fecal samples, out of which 50% (5/10) of the positive samples were characterized into the genogroups GI.1 (1), GI.2 (2), and GII.4 (2). These findings highlighted the important contributions of AdV, HAstV, and SaV in the enteric virus spectrum in our region and showed the high genetic diversity of AdV. In addition, it demonstrated for the first time in Brazil, the circulation of AdV in the serum of hospitalized children with AGE. |
publishDate |
2018 |
dc.date.accessioned.fl_str_mv |
2018-11-13T19:46:19Z |
dc.date.available.fl_str_mv |
2018-11-13T19:46:19Z |
dc.date.issued.fl_str_mv |
2019 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
PORTAL, Thayara Morais et al. Detection and genotyping of enteric viruses in hospitalized children with acute gastroenteritis in Belém, Brazil: occurrence of adenovirus viremia by species F, types 40/41. Journal of Medical Virology, v. 91, n. 3, p. 378-384, Jan. 2019. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/3529 |
dc.identifier.issn.-.fl_str_mv |
0146-6615 |
dc.identifier.doi.-.fl_str_mv |
10.1002/jmv.25321 |
identifier_str_mv |
PORTAL, Thayara Morais et al. Detection and genotyping of enteric viruses in hospitalized children with acute gastroenteritis in Belém, Brazil: occurrence of adenovirus viremia by species F, types 40/41. Journal of Medical Virology, v. 91, n. 3, p. 378-384, Jan. 2019. 0146-6615 10.1002/jmv.25321 |
url |
https://patua.iec.gov.br/handle/iec/3529 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
reponame:Repositório Digital do Instituto Evandro Chagas (Patuá) instname:Instituto Evandro Chagas (IEC) instacron:IEC |
instname_str |
Instituto Evandro Chagas (IEC) |
instacron_str |
IEC |
institution |
IEC |
reponame_str |
Repositório Digital do Instituto Evandro Chagas (Patuá) |
collection |
Repositório Digital do Instituto Evandro Chagas (Patuá) |
bitstream.url.fl_str_mv |
https://patua.iec.gov.br/bitstreams/dfb042b5-44a0-48d5-b953-497db329c60e/download https://patua.iec.gov.br/bitstreams/8ecb6bac-30ea-4e34-94c1-67ac30a46cba/download https://patua.iec.gov.br/bitstreams/737cf747-f8cf-4156-886c-cda327854465/download https://patua.iec.gov.br/bitstreams/86700d43-c694-474b-ba9a-6381b4139dd7/download |
bitstream.checksum.fl_str_mv |
6a58d733bbe123f9a6f3d02aa8287a0d 52f1732ea66fbd1123abe39f5373b797 81a0e12b439a341593fb50aeb89768a7 09df96cf9b54b88bd62ffba9101062da |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC) |
repository.mail.fl_str_mv |
clariceneta@iec.gov.br || Biblioteca@iec.gov.br |
_version_ |
1809190042511343616 |