Detection and genotyping of enteric viruses in hospitalized children with acute gastroenteritis in Belém, Brazil: occurrence of adenovirus viremia by species F, types 40/41

Detalhes bibliográficos
Autor(a) principal: Portal, Thayara Morais
Data de Publicação: 2018
Outros Autores: Reymão, Tammy Kathlyn Amaral, Quinderé Neto, Germano Alves, Fiuza, Mercedes Kamila da C, Teixeira, Dielle Monteiro, Lima, Ian Carlos Gomes, Sousa Júnior, Edivaldo Costa, Bandeira, Renato da Silva, Deus, Danielle Rodrigues de, Justino, Maria Cleonice Aguiar, Linhares, Alexandre da Costa, Silva, Luciana Damascena da, Resque, Hugo Reis, Gabbay, Yvone Benchimol
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
Texto Completo: https://patua.iec.gov.br/handle/iec/3529
Resumo: Enteric adenovirus (AdV), sapovirus (SaV), and human astrovirus (HAstV) are important pathogens involved in the gastroenteritis etiology. In this study, a total of 219 fecal samples and sera were collected from children hospitalized for acute gastroenteritis (AGE) in two large pediatric hospitals in Belém, from March 2012 to April 2015. The samples were analyzed by polymerase chain reaction (PCR) for adenovirus (AdV) and HAstV (astrovirus) detection, and Nested-PCR and qPCR for sapovirus (SaV) detection. AdV was detected in 50.2% (110/219) of the cases, with 42.7% (47/110) being sequenced and classified as: species F (63.9% - 30/47), A (4.2% - 2/47), B (6.4% - 3/47), C (17.1% - 8/47), D (4.2% - 2/47), and E (4.2% - 2/47). Of the 110 AdV-positive faeces samples, 80 paired sera presented sufficient amounts and were also tested for this virus, of which 51 (63.7%) showed positive results and 26 (70.3%) pairs (feces plus sera) presented concordant results after sequencing being classified as: species F (21/26; 80.8%), A (1/26; 3.8%), B (1/26; 3.8%), and C (3/26; 11.5%). Overall, HAstV rate in the feces samples was 1.8% (4/219), including both HAstV-1a (2/4; 50%) and HAstV-2c (2/4; 50%). SaV was detected in 4.6% (10/219) of the fecal samples, out of which 50% (5/10) of the positive samples were characterized into the genogroups GI.1 (1), GI.2 (2), and GII.4 (2). These findings highlighted the important contributions of AdV, HAstV, and SaV in the enteric virus spectrum in our region and showed the high genetic diversity of AdV. In addition, it demonstrated for the first time in Brazil, the circulation of AdV in the serum of hospitalized children with AGE.
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spelling Portal, Thayara MoraisReymão, Tammy Kathlyn AmaralQuinderé Neto, Germano AlvesFiuza, Mercedes Kamila da CTeixeira, Dielle MonteiroLima, Ian Carlos GomesSousa Júnior, Edivaldo CostaBandeira, Renato da SilvaDeus, Danielle Rodrigues deJustino, Maria Cleonice AguiarLinhares, Alexandre da CostaSilva, Luciana Damascena daResque, Hugo ReisGabbay, Yvone Benchimol2018-11-13T19:46:19Z2018-11-13T19:46:19Z2019PORTAL, Thayara Morais et al. Detection and genotyping of enteric viruses in hospitalized children with acute gastroenteritis in Belém, Brazil: occurrence of adenovirus viremia by species F, types 40/41. Journal of Medical Virology, v. 91, n. 3, p. 378-384, Jan. 2019.0146-6615https://patua.iec.gov.br/handle/iec/352910.1002/jmv.25321Enteric adenovirus (AdV), sapovirus (SaV), and human astrovirus (HAstV) are important pathogens involved in the gastroenteritis etiology. In this study, a total of 219 fecal samples and sera were collected from children hospitalized for acute gastroenteritis (AGE) in two large pediatric hospitals in Belém, from March 2012 to April 2015. The samples were analyzed by polymerase chain reaction (PCR) for adenovirus (AdV) and HAstV (astrovirus) detection, and Nested-PCR and qPCR for sapovirus (SaV) detection. AdV was detected in 50.2% (110/219) of the cases, with 42.7% (47/110) being sequenced and classified as: species F (63.9% - 30/47), A (4.2% - 2/47), B (6.4% - 3/47), C (17.1% - 8/47), D (4.2% - 2/47), and E (4.2% - 2/47). Of the 110 AdV-positive faeces samples, 80 paired sera presented sufficient amounts and were also tested for this virus, of which 51 (63.7%) showed positive results and 26 (70.3%) pairs (feces plus sera) presented concordant results after sequencing being classified as: species F (21/26; 80.8%), A (1/26; 3.8%), B (1/26; 3.8%), and C (3/26; 11.5%). Overall, HAstV rate in the feces samples was 1.8% (4/219), including both HAstV-1a (2/4; 50%) and HAstV-2c (2/4; 50%). SaV was detected in 4.6% (10/219) of the fecal samples, out of which 50% (5/10) of the positive samples were characterized into the genogroups GI.1 (1), GI.2 (2), and GII.4 (2). These findings highlighted the important contributions of AdV, HAstV, and SaV in the enteric virus spectrum in our region and showed the high genetic diversity of AdV. In addition, it demonstrated for the first time in Brazil, the circulation of AdV in the serum of hospitalized children with AGE.This research was supported by Evandro Chagas Institute; Capes.University of State of Pará. Postgraduate Program in Parasitic Biology in the Amazon. Belém, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas.Program de Pós-Graduação em Virologia. Ananindeua, PA, Brasil.University of State of Pará. Undergraduate in Nursing. Belém, PA, Brazil.School of the Amazon. Belém, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.University of State of Pará. Postgraduate Program in Parasitic Biology in the Amazon. Belém, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.engWileyDetection and genotyping of enteric viruses in hospitalized children with acute gastroenteritis in Belém, Brazil: occurrence of adenovirus viremia by species F, types 40/41info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGastroenterite / patologiaInfecções por Adenovirus HumanosAdenovírus Humanos / patogenicidadeSapovirusMamastrovirusDiarreia Infantil / patologiaReação em Cadeia da Polimerase / métodosinfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALDetection and genotyping of enteric viruses in hospitalized children with acute gastroenteritis in Belém, Brazil: Occurrence of adenovirus viremia by species F, types 40/41.pdfDetection and genotyping of enteric viruses in hospitalized children with acute gastroenteritis in Belém, Brazil: Occurrence of adenovirus viremia by species F, types 40/41.pdfapplication/pdf842080https://patua.iec.gov.br/bitstreams/dfb042b5-44a0-48d5-b953-497db329c60e/download6a58d733bbe123f9a6f3d02aa8287a0dMD53LICENSElicense.txtlicense.txttext/plain; charset=utf-871https://patua.iec.gov.br/bitstreams/8ecb6bac-30ea-4e34-94c1-67ac30a46cba/download52f1732ea66fbd1123abe39f5373b797MD52TEXTDetection and genotyping of enteric viruses in hospitalized children with acute gastroenteritis in Belém, Brazil: Occurrence of adenovirus viremia by species F, types 40/41.pdf.txtDetection and genotyping of enteric viruses in hospitalized children with acute gastroenteritis in Belém, Brazil: Occurrence of adenovirus viremia by species F, types 40/41.pdf.txtExtracted texttext/plain37712https://patua.iec.gov.br/bitstreams/737cf747-f8cf-4156-886c-cda327854465/download81a0e12b439a341593fb50aeb89768a7MD56THUMBNAILDetection and genotyping of enteric viruses in hospitalized children with acute gastroenteritis in Belém, Brazil: Occurrence of adenovirus viremia by species F, types 40/41.pdf.jpgDetection and genotyping of enteric viruses in hospitalized children with acute gastroenteritis in Belém, Brazil: Occurrence of adenovirus viremia by species F, types 40/41.pdf.jpgGenerated Thumbnailimage/jpeg6463https://patua.iec.gov.br/bitstreams/86700d43-c694-474b-ba9a-6381b4139dd7/download09df96cf9b54b88bd62ffba9101062daMD57iec/35292022-10-20 21:44:45.98oai:patua.iec.gov.br:iec/3529https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-20T21:44:45Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)falseVG9kb3Mgb3MgZG9jdW1lbnRvcyBkZXNzYSBjb2xlw6fDo28gc2VndWVtIGEgTGljZW7Dp2EgQ3JlYXRpdmUgY29tbW9ucy4=
dc.title.pt_BR.fl_str_mv Detection and genotyping of enteric viruses in hospitalized children with acute gastroenteritis in Belém, Brazil: occurrence of adenovirus viremia by species F, types 40/41
title Detection and genotyping of enteric viruses in hospitalized children with acute gastroenteritis in Belém, Brazil: occurrence of adenovirus viremia by species F, types 40/41
spellingShingle Detection and genotyping of enteric viruses in hospitalized children with acute gastroenteritis in Belém, Brazil: occurrence of adenovirus viremia by species F, types 40/41
Portal, Thayara Morais
Gastroenterite / patologia
Infecções por Adenovirus Humanos
Adenovírus Humanos / patogenicidade
Sapovirus
Mamastrovirus
Diarreia Infantil / patologia
Reação em Cadeia da Polimerase / métodos
title_short Detection and genotyping of enteric viruses in hospitalized children with acute gastroenteritis in Belém, Brazil: occurrence of adenovirus viremia by species F, types 40/41
title_full Detection and genotyping of enteric viruses in hospitalized children with acute gastroenteritis in Belém, Brazil: occurrence of adenovirus viremia by species F, types 40/41
title_fullStr Detection and genotyping of enteric viruses in hospitalized children with acute gastroenteritis in Belém, Brazil: occurrence of adenovirus viremia by species F, types 40/41
title_full_unstemmed Detection and genotyping of enteric viruses in hospitalized children with acute gastroenteritis in Belém, Brazil: occurrence of adenovirus viremia by species F, types 40/41
title_sort Detection and genotyping of enteric viruses in hospitalized children with acute gastroenteritis in Belém, Brazil: occurrence of adenovirus viremia by species F, types 40/41
author Portal, Thayara Morais
author_facet Portal, Thayara Morais
Reymão, Tammy Kathlyn Amaral
Quinderé Neto, Germano Alves
Fiuza, Mercedes Kamila da C
Teixeira, Dielle Monteiro
Lima, Ian Carlos Gomes
Sousa Júnior, Edivaldo Costa
Bandeira, Renato da Silva
Deus, Danielle Rodrigues de
Justino, Maria Cleonice Aguiar
Linhares, Alexandre da Costa
Silva, Luciana Damascena da
Resque, Hugo Reis
Gabbay, Yvone Benchimol
author_role author
author2 Reymão, Tammy Kathlyn Amaral
Quinderé Neto, Germano Alves
Fiuza, Mercedes Kamila da C
Teixeira, Dielle Monteiro
Lima, Ian Carlos Gomes
Sousa Júnior, Edivaldo Costa
Bandeira, Renato da Silva
Deus, Danielle Rodrigues de
Justino, Maria Cleonice Aguiar
Linhares, Alexandre da Costa
Silva, Luciana Damascena da
Resque, Hugo Reis
Gabbay, Yvone Benchimol
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Portal, Thayara Morais
Reymão, Tammy Kathlyn Amaral
Quinderé Neto, Germano Alves
Fiuza, Mercedes Kamila da C
Teixeira, Dielle Monteiro
Lima, Ian Carlos Gomes
Sousa Júnior, Edivaldo Costa
Bandeira, Renato da Silva
Deus, Danielle Rodrigues de
Justino, Maria Cleonice Aguiar
Linhares, Alexandre da Costa
Silva, Luciana Damascena da
Resque, Hugo Reis
Gabbay, Yvone Benchimol
dc.subject.decsPrimary.pt_BR.fl_str_mv Gastroenterite / patologia
Infecções por Adenovirus Humanos
Adenovírus Humanos / patogenicidade
Sapovirus
Mamastrovirus
Diarreia Infantil / patologia
Reação em Cadeia da Polimerase / métodos
topic Gastroenterite / patologia
Infecções por Adenovirus Humanos
Adenovírus Humanos / patogenicidade
Sapovirus
Mamastrovirus
Diarreia Infantil / patologia
Reação em Cadeia da Polimerase / métodos
description Enteric adenovirus (AdV), sapovirus (SaV), and human astrovirus (HAstV) are important pathogens involved in the gastroenteritis etiology. In this study, a total of 219 fecal samples and sera were collected from children hospitalized for acute gastroenteritis (AGE) in two large pediatric hospitals in Belém, from March 2012 to April 2015. The samples were analyzed by polymerase chain reaction (PCR) for adenovirus (AdV) and HAstV (astrovirus) detection, and Nested-PCR and qPCR for sapovirus (SaV) detection. AdV was detected in 50.2% (110/219) of the cases, with 42.7% (47/110) being sequenced and classified as: species F (63.9% - 30/47), A (4.2% - 2/47), B (6.4% - 3/47), C (17.1% - 8/47), D (4.2% - 2/47), and E (4.2% - 2/47). Of the 110 AdV-positive faeces samples, 80 paired sera presented sufficient amounts and were also tested for this virus, of which 51 (63.7%) showed positive results and 26 (70.3%) pairs (feces plus sera) presented concordant results after sequencing being classified as: species F (21/26; 80.8%), A (1/26; 3.8%), B (1/26; 3.8%), and C (3/26; 11.5%). Overall, HAstV rate in the feces samples was 1.8% (4/219), including both HAstV-1a (2/4; 50%) and HAstV-2c (2/4; 50%). SaV was detected in 4.6% (10/219) of the fecal samples, out of which 50% (5/10) of the positive samples were characterized into the genogroups GI.1 (1), GI.2 (2), and GII.4 (2). These findings highlighted the important contributions of AdV, HAstV, and SaV in the enteric virus spectrum in our region and showed the high genetic diversity of AdV. In addition, it demonstrated for the first time in Brazil, the circulation of AdV in the serum of hospitalized children with AGE.
publishDate 2018
dc.date.accessioned.fl_str_mv 2018-11-13T19:46:19Z
dc.date.available.fl_str_mv 2018-11-13T19:46:19Z
dc.date.issued.fl_str_mv 2019
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.citation.fl_str_mv PORTAL, Thayara Morais et al. Detection and genotyping of enteric viruses in hospitalized children with acute gastroenteritis in Belém, Brazil: occurrence of adenovirus viremia by species F, types 40/41. Journal of Medical Virology, v. 91, n. 3, p. 378-384, Jan. 2019.
dc.identifier.uri.fl_str_mv https://patua.iec.gov.br/handle/iec/3529
dc.identifier.issn.-.fl_str_mv 0146-6615
dc.identifier.doi.-.fl_str_mv 10.1002/jmv.25321
identifier_str_mv PORTAL, Thayara Morais et al. Detection and genotyping of enteric viruses in hospitalized children with acute gastroenteritis in Belém, Brazil: occurrence of adenovirus viremia by species F, types 40/41. Journal of Medical Virology, v. 91, n. 3, p. 378-384, Jan. 2019.
0146-6615
10.1002/jmv.25321
url https://patua.iec.gov.br/handle/iec/3529
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