Molecular analysis of G3P[6] rotavirus in the Amazon region of Brazil: evidence of reassortment with equine-like strains

Detalhes bibliográficos
Autor(a) principal: Serra, Ana C. Silva
Data de Publicação: 2021
Outros Autores: Sousa Júnior, Edivaldo costa, Cruz, Jonas F, Lobo, Patrícia dos Santos, Penha Júnior, Edvaldo Tavares da, Bandeira, Renato S, Bezerra, Delana Andreza Melo, Mascarenhas, Joana D’Arc Pereira, Guerra, Sylvia de Fátima dos Santos, Soares, Luana da Silva
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
Texto Completo: https://patua.iec.gov.br/handle/iec/4393
Resumo: Aim: To perform a molecular analysis of rotavirus A (RVA) G3P[6] strains detected in 2012 and 2017 in the Amazon region of Brazil. Materials & methods: Eighteen RVA G3P[6] strains were collected from children aged under 10 years hospitalized with acute gastroenteritis, and partial sequencing of each segment genome was performed using Sanger sequencing. Results: Phylogenetic analysis showed that all G3P[6] strains had a DS-1-like genotype constellation. Two strains had the highest nucleotide identities with equine-like G3P[6]/G3P[8] genotypes. Several amino acid alterations in VP4 and VP7 neutralizing epitopes of equine-like RVA G3P[6] strains were observed in comparison with vaccine strains. Conclusion: These findings suggest that equine-like RVA G3P[6] strains have been circulating in the Amazon region of Brazil as a result of direct importation, and support natural RVA evolutionary mechanisms.
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spelling Serra, Ana C. SilvaSousa Júnior, Edivaldo costaCruz, Jonas FLobo, Patrícia dos SantosPenha Júnior, Edvaldo Tavares daBandeira, Renato SBezerra, Delana Andreza MeloMascarenhas, Joana D’Arc PereiraGuerra, Sylvia de Fátima dos SantosSoares, Luana da Silva2021-08-10T11:13:51Z2021-08-10T11:13:51Z2021SERRA, Ana C. Silva et al. Molecular analysis of G3P[6] rotavirus in the Amazon region of Brazil: evidence of reassortment with equine-like strains. Future Microbiology, v. 16, n. 12, p. 847-862, July 2021.1746-0913https://patua.iec.gov.br/handle/iec/439310.2217/fmb-2020-0002Aim: To perform a molecular analysis of rotavirus A (RVA) G3P[6] strains detected in 2012 and 2017 in the Amazon region of Brazil. Materials & methods: Eighteen RVA G3P[6] strains were collected from children aged under 10 years hospitalized with acute gastroenteritis, and partial sequencing of each segment genome was performed using Sanger sequencing. Results: Phylogenetic analysis showed that all G3P[6] strains had a DS-1-like genotype constellation. Two strains had the highest nucleotide identities with equine-like G3P[6]/G3P[8] genotypes. Several amino acid alterations in VP4 and VP7 neutralizing epitopes of equine-like RVA G3P[6] strains were observed in comparison with vaccine strains. Conclusion: These findings suggest that equine-like RVA G3P[6] strains have been circulating in the Amazon region of Brazil as a result of direct importation, and support natural RVA evolutionary mechanisms.Instituto Evandro Chagas. Secretaria de Vigilância em Saúde. Ministério da Saúde.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Programa de Pós-Graduação em Virologia. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.engFuture Science GroupMolecular analysis of G3P[6] rotavirus in the Amazon region of Brazil: evidence of reassortment with equine-like strainsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleRotavirus / isolamento & purificaçãoDiarreia / virologiaGastroenterite / patologiaFilogeniaBetacoronavirus 1 / patogenicidadeGenótipoinfo:eu-repo/semantics/embargoedAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALMolecular analysis of G3P[6] rotavirus in the Amazon region of Brazil: evidence of reassortment with equine-like strains.pdfMolecular analysis of G3P[6] rotavirus in the Amazon region of Brazil: evidence of reassortment with equine-like strains.pdfapplication/pdf551083https://patua.iec.gov.br/bitstreams/26620407-7454-47bd-9bd1-88c9280e7d7d/downloadc9a9c128e29cac82a5d7fdf3f4e6da73MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82182https://patua.iec.gov.br/bitstreams/8e125689-3d5b-4b61-8e92-d737b2a1b831/download11832eea31b16df8613079d742d61793MD52TEXTMolecular analysis of G3P[6] rotavirus in the Amazon region of Brazil: evidence of reassortment with equine-like strains.pdf.txtMolecular analysis of G3P[6] rotavirus in the Amazon region of Brazil: evidence of reassortment with equine-like strains.pdf.txtExtracted texttext/plain2https://patua.iec.gov.br/bitstreams/cb59a091-0404-4742-819d-2ba68999347c/downloade1c06d85ae7b8b032bef47e42e4c08f9MD55THUMBNAILMolecular analysis of G3P[6] rotavirus in the Amazon region of Brazil: evidence of reassortment with equine-like strains.pdf.jpgMolecular analysis of G3P[6] rotavirus in the Amazon region of Brazil: evidence of reassortment with equine-like strains.pdf.jpgGenerated Thumbnailimage/jpeg3095https://patua.iec.gov.br/bitstreams/4e448b5b-e8fb-4ad7-acb4-db5088bba0f5/download71859d578212107f7f8c49a4ce09d9eeMD56iec/43932022-10-20 22:37:52.343oai:patua.iec.gov.br:iec/4393https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-20T22:37:52Repositório Digital do Instituto Evandro Chagas (Patuá) - 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dc.title.pt_BR.fl_str_mv Molecular analysis of G3P[6] rotavirus in the Amazon region of Brazil: evidence of reassortment with equine-like strains
title Molecular analysis of G3P[6] rotavirus in the Amazon region of Brazil: evidence of reassortment with equine-like strains
spellingShingle Molecular analysis of G3P[6] rotavirus in the Amazon region of Brazil: evidence of reassortment with equine-like strains
Serra, Ana C. Silva
Rotavirus / isolamento & purificação
Diarreia / virologia
Gastroenterite / patologia
Filogenia
Betacoronavirus 1 / patogenicidade
Genótipo
title_short Molecular analysis of G3P[6] rotavirus in the Amazon region of Brazil: evidence of reassortment with equine-like strains
title_full Molecular analysis of G3P[6] rotavirus in the Amazon region of Brazil: evidence of reassortment with equine-like strains
title_fullStr Molecular analysis of G3P[6] rotavirus in the Amazon region of Brazil: evidence of reassortment with equine-like strains
title_full_unstemmed Molecular analysis of G3P[6] rotavirus in the Amazon region of Brazil: evidence of reassortment with equine-like strains
title_sort Molecular analysis of G3P[6] rotavirus in the Amazon region of Brazil: evidence of reassortment with equine-like strains
author Serra, Ana C. Silva
author_facet Serra, Ana C. Silva
Sousa Júnior, Edivaldo costa
Cruz, Jonas F
Lobo, Patrícia dos Santos
Penha Júnior, Edvaldo Tavares da
Bandeira, Renato S
Bezerra, Delana Andreza Melo
Mascarenhas, Joana D’Arc Pereira
Guerra, Sylvia de Fátima dos Santos
Soares, Luana da Silva
author_role author
author2 Sousa Júnior, Edivaldo costa
Cruz, Jonas F
Lobo, Patrícia dos Santos
Penha Júnior, Edvaldo Tavares da
Bandeira, Renato S
Bezerra, Delana Andreza Melo
Mascarenhas, Joana D’Arc Pereira
Guerra, Sylvia de Fátima dos Santos
Soares, Luana da Silva
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Serra, Ana C. Silva
Sousa Júnior, Edivaldo costa
Cruz, Jonas F
Lobo, Patrícia dos Santos
Penha Júnior, Edvaldo Tavares da
Bandeira, Renato S
Bezerra, Delana Andreza Melo
Mascarenhas, Joana D’Arc Pereira
Guerra, Sylvia de Fátima dos Santos
Soares, Luana da Silva
dc.subject.decsPrimary.pt_BR.fl_str_mv Rotavirus / isolamento & purificação
Diarreia / virologia
Gastroenterite / patologia
Filogenia
Betacoronavirus 1 / patogenicidade
Genótipo
topic Rotavirus / isolamento & purificação
Diarreia / virologia
Gastroenterite / patologia
Filogenia
Betacoronavirus 1 / patogenicidade
Genótipo
description Aim: To perform a molecular analysis of rotavirus A (RVA) G3P[6] strains detected in 2012 and 2017 in the Amazon region of Brazil. Materials & methods: Eighteen RVA G3P[6] strains were collected from children aged under 10 years hospitalized with acute gastroenteritis, and partial sequencing of each segment genome was performed using Sanger sequencing. Results: Phylogenetic analysis showed that all G3P[6] strains had a DS-1-like genotype constellation. Two strains had the highest nucleotide identities with equine-like G3P[6]/G3P[8] genotypes. Several amino acid alterations in VP4 and VP7 neutralizing epitopes of equine-like RVA G3P[6] strains were observed in comparison with vaccine strains. Conclusion: These findings suggest that equine-like RVA G3P[6] strains have been circulating in the Amazon region of Brazil as a result of direct importation, and support natural RVA evolutionary mechanisms.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-08-10T11:13:51Z
dc.date.available.fl_str_mv 2021-08-10T11:13:51Z
dc.date.issued.fl_str_mv 2021
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SERRA, Ana C. Silva et al. Molecular analysis of G3P[6] rotavirus in the Amazon region of Brazil: evidence of reassortment with equine-like strains. Future Microbiology, v. 16, n. 12, p. 847-862, July 2021.
dc.identifier.uri.fl_str_mv https://patua.iec.gov.br/handle/iec/4393
dc.identifier.issn.-.fl_str_mv 1746-0913
dc.identifier.doi.-.fl_str_mv 10.2217/fmb-2020-0002
identifier_str_mv SERRA, Ana C. Silva et al. Molecular analysis of G3P[6] rotavirus in the Amazon region of Brazil: evidence of reassortment with equine-like strains. Future Microbiology, v. 16, n. 12, p. 847-862, July 2021.
1746-0913
10.2217/fmb-2020-0002
url https://patua.iec.gov.br/handle/iec/4393
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/embargoedAccess
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dc.publisher.none.fl_str_mv Future Science Group
publisher.none.fl_str_mv Future Science Group
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