Rotavirus A in wild and domestic animals from areas with environmental degradation in the Brazilian Amazon
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/3601 |
Resumo: | Acute gastroenteritis is one of the main causes of mortality in humans and young animals. Domestic and mainly wild animals such as bats, small rodents and birds are highly diversified animals in relation to their habitats and ecological niches and are widely distributed geographically in environments of forest fragmentation in some areas of the Amazon, being considered important sources for viruses that affect humans and other animals. Due to the anthropical activities, these animals changed their natural habitat and adapted to urbanized environments, thus representing risks to human and animal health. Although the knowledge of the global diversity of enteric viruses is scarce, there are reports demonstrating the detection of rotavirus in domestic animals and animals of productive systems, such as bovines and pigs. The present study investigated the prevalence of Rotavirus A in 648 fecal samples of different animal species from the northeastern mesoregion of the state of Para´, Brazil, which is characterized as an urbanized area with forest fragments. The fecal specimens were collected from October 2014 to April 2016 and subjected to a Qualitative Real-Time Polymerase Chain Reaction (RT-qPCR), using the NSP3 gene as a target. It was observed that 27.5% (178/648) of the samples presented positive results for RVA, with 178 samples distributed in birds (23.6%), canines (21.35%), chiropterans (17.98%), bovines (14.6%), horses (8.43%), small rodents (6.74%), pigs (3.93%) and felines (3.37%), demonstrating the circulation of RVA in domestic animals and suggesting that such proximity could cause transmissions between different species and the occurrence of rearrangements in the genome of RVA as already described in the literature, associated to the traces of environmental degradation in the studied areas. |
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Barros, Bruno de Cássio Veloso deChagas, Elaine NunesBezerra, Luna WanessaRibeiro, Laila GrazielaDuarte Júnior, Jose Wandilson BarbozaPereira, DiegoPenha Junior, Edvaldo Tavares daSilva, Julia RezendeBezerra, Delana Andreza MeloBandeira, Renata SilvaPinheiro, Helder Henrique CostaGuerra, Sylvia de Fátima dos SantosGuimarães, Ricardo José de Paula Souza eMascarenhas, Joana D'Arc Pereira2019-02-20T18:26:48Z2019-02-20T18:26:48Z2018BARROS, Bruno de Cássio Veloso de et al. Rotavirus A in wild and domestic animals from areas with environmental degradation in the Brazilian Amazon. PLoS ONE, v. 13, n. 12, e0209005, p. 1-18, Dec. 2018.1932-6203https://patua.iec.gov.br/handle/iec/360110.1371/journal.pone.0209005Acute gastroenteritis is one of the main causes of mortality in humans and young animals. Domestic and mainly wild animals such as bats, small rodents and birds are highly diversified animals in relation to their habitats and ecological niches and are widely distributed geographically in environments of forest fragmentation in some areas of the Amazon, being considered important sources for viruses that affect humans and other animals. Due to the anthropical activities, these animals changed their natural habitat and adapted to urbanized environments, thus representing risks to human and animal health. Although the knowledge of the global diversity of enteric viruses is scarce, there are reports demonstrating the detection of rotavirus in domestic animals and animals of productive systems, such as bovines and pigs. The present study investigated the prevalence of Rotavirus A in 648 fecal samples of different animal species from the northeastern mesoregion of the state of Para´, Brazil, which is characterized as an urbanized area with forest fragments. The fecal specimens were collected from October 2014 to April 2016 and subjected to a Qualitative Real-Time Polymerase Chain Reaction (RT-qPCR), using the NSP3 gene as a target. It was observed that 27.5% (178/648) of the samples presented positive results for RVA, with 178 samples distributed in birds (23.6%), canines (21.35%), chiropterans (17.98%), bovines (14.6%), horses (8.43%), small rodents (6.74%), pigs (3.93%) and felines (3.37%), demonstrating the circulation of RVA in domestic animals and suggesting that such proximity could cause transmissions between different species and the occurrence of rearrangements in the genome of RVA as already described in the literature, associated to the traces of environmental degradation in the studied areas.This research was supported by the Coordination for the Improvement of Higher Education Personnel (CAPES) n. 2303800717 / 2013-52, National Council for Scientific and Technological Development (CNPq) and Evandro Chagas Institute, Ministry of Health, Ananindeua, Brazil. BARROS BCV is a beneficiary of the Capes grant; Chagas EHN and Mascarenhas JDP are recipients of grants from CNPq. The authors would like to acknowledge the Amazon Support Foundation of the studies and research (FAPESPA) for funding (ICAAF166/2014).Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Amazon Metropolitan University Center. Belém, PA, Brazil.Amazon Metropolitan University Center. Belém, PA, Brazil.Amazon Metropolitan University Center. Belém, PA, Brazil.Amazon Metropolitan University Center. Belém, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.University of the State of Pará. Belém, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Federal University of Pará. Belém, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Amazon Metropolitan University Center. Belém, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. 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dc.title.pt_BR.fl_str_mv |
Rotavirus A in wild and domestic animals from areas with environmental degradation in the Brazilian Amazon |
title |
Rotavirus A in wild and domestic animals from areas with environmental degradation in the Brazilian Amazon |
spellingShingle |
Rotavirus A in wild and domestic animals from areas with environmental degradation in the Brazilian Amazon Barros, Bruno de Cássio Veloso de Rotavirus / isolamento & purificação Rotavirus / patogenicidade Animais Domésticos / virologia Animais Selvagens / virologia Fezes / virologia Reação em Cadeia da Polimerase em Tempo Real / métodos Ecossistema Meio Ambiente Alteração Ambiental |
title_short |
Rotavirus A in wild and domestic animals from areas with environmental degradation in the Brazilian Amazon |
title_full |
Rotavirus A in wild and domestic animals from areas with environmental degradation in the Brazilian Amazon |
title_fullStr |
Rotavirus A in wild and domestic animals from areas with environmental degradation in the Brazilian Amazon |
title_full_unstemmed |
Rotavirus A in wild and domestic animals from areas with environmental degradation in the Brazilian Amazon |
title_sort |
Rotavirus A in wild and domestic animals from areas with environmental degradation in the Brazilian Amazon |
author |
Barros, Bruno de Cássio Veloso de |
author_facet |
Barros, Bruno de Cássio Veloso de Chagas, Elaine Nunes Bezerra, Luna Wanessa Ribeiro, Laila Graziela Duarte Júnior, Jose Wandilson Barboza Pereira, Diego Penha Junior, Edvaldo Tavares da Silva, Julia Rezende Bezerra, Delana Andreza Melo Bandeira, Renata Silva Pinheiro, Helder Henrique Costa Guerra, Sylvia de Fátima dos Santos Guimarães, Ricardo José de Paula Souza e Mascarenhas, Joana D'Arc Pereira |
author_role |
author |
author2 |
Chagas, Elaine Nunes Bezerra, Luna Wanessa Ribeiro, Laila Graziela Duarte Júnior, Jose Wandilson Barboza Pereira, Diego Penha Junior, Edvaldo Tavares da Silva, Julia Rezende Bezerra, Delana Andreza Melo Bandeira, Renata Silva Pinheiro, Helder Henrique Costa Guerra, Sylvia de Fátima dos Santos Guimarães, Ricardo José de Paula Souza e Mascarenhas, Joana D'Arc Pereira |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Barros, Bruno de Cássio Veloso de Chagas, Elaine Nunes Bezerra, Luna Wanessa Ribeiro, Laila Graziela Duarte Júnior, Jose Wandilson Barboza Pereira, Diego Penha Junior, Edvaldo Tavares da Silva, Julia Rezende Bezerra, Delana Andreza Melo Bandeira, Renata Silva Pinheiro, Helder Henrique Costa Guerra, Sylvia de Fátima dos Santos Guimarães, Ricardo José de Paula Souza e Mascarenhas, Joana D'Arc Pereira |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Rotavirus / isolamento & purificação Rotavirus / patogenicidade Animais Domésticos / virologia Animais Selvagens / virologia Fezes / virologia Reação em Cadeia da Polimerase em Tempo Real / métodos Ecossistema Meio Ambiente |
topic |
Rotavirus / isolamento & purificação Rotavirus / patogenicidade Animais Domésticos / virologia Animais Selvagens / virologia Fezes / virologia Reação em Cadeia da Polimerase em Tempo Real / métodos Ecossistema Meio Ambiente Alteração Ambiental |
dc.subject.decsPrimary.none.fl_str_mv |
Alteração Ambiental |
description |
Acute gastroenteritis is one of the main causes of mortality in humans and young animals. Domestic and mainly wild animals such as bats, small rodents and birds are highly diversified animals in relation to their habitats and ecological niches and are widely distributed geographically in environments of forest fragmentation in some areas of the Amazon, being considered important sources for viruses that affect humans and other animals. Due to the anthropical activities, these animals changed their natural habitat and adapted to urbanized environments, thus representing risks to human and animal health. Although the knowledge of the global diversity of enteric viruses is scarce, there are reports demonstrating the detection of rotavirus in domestic animals and animals of productive systems, such as bovines and pigs. The present study investigated the prevalence of Rotavirus A in 648 fecal samples of different animal species from the northeastern mesoregion of the state of Para´, Brazil, which is characterized as an urbanized area with forest fragments. The fecal specimens were collected from October 2014 to April 2016 and subjected to a Qualitative Real-Time Polymerase Chain Reaction (RT-qPCR), using the NSP3 gene as a target. It was observed that 27.5% (178/648) of the samples presented positive results for RVA, with 178 samples distributed in birds (23.6%), canines (21.35%), chiropterans (17.98%), bovines (14.6%), horses (8.43%), small rodents (6.74%), pigs (3.93%) and felines (3.37%), demonstrating the circulation of RVA in domestic animals and suggesting that such proximity could cause transmissions between different species and the occurrence of rearrangements in the genome of RVA as already described in the literature, associated to the traces of environmental degradation in the studied areas. |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018 |
dc.date.accessioned.fl_str_mv |
2019-02-20T18:26:48Z |
dc.date.available.fl_str_mv |
2019-02-20T18:26:48Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
BARROS, Bruno de Cássio Veloso de et al. Rotavirus A in wild and domestic animals from areas with environmental degradation in the Brazilian Amazon. PLoS ONE, v. 13, n. 12, e0209005, p. 1-18, Dec. 2018. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/3601 |
dc.identifier.issn.-.fl_str_mv |
1932-6203 |
dc.identifier.doi.-.fl_str_mv |
10.1371/journal.pone.0209005 |
identifier_str_mv |
BARROS, Bruno de Cássio Veloso de et al. Rotavirus A in wild and domestic animals from areas with environmental degradation in the Brazilian Amazon. PLoS ONE, v. 13, n. 12, e0209005, p. 1-18, Dec. 2018. 1932-6203 10.1371/journal.pone.0209005 |
url |
https://patua.iec.gov.br/handle/iec/3601 |
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eng |
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eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
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Public Library of Science |
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Public Library of Science |
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