Detection of Epstein-Barr virus in gastric adenocarcinoma: qPCR and fish comparison

Detalhes bibliográficos
Autor(a) principal: Costa, Igor Brasil
Data de Publicação: 2022
Outros Autores: Souza, Carolina Rosal Teixeira de, Costa, Iran Barros, Santos, Liann Filiphe Pereira dos, Paixão, Luana César Ferraz, Polaro, Alessandra Alves, Monteiro, Talita Antônia Furtado, Burbano, Rommel Mário Rodríguez
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
Texto Completo: https://patua.iec.gov.br/handle/iec/4495
Resumo: EBV-associated gastric cancer accounts for about 10% of all gastric carcinomas worldwide. We aimed to verify the prevalence of EBV in gastric adenocarcinoma samples using FISH and qPCR and comparing the results obtained by both techniques. Gastric cancer samples from 191 cases were analyzed. The FISH assay was performed to detect small EBV RNAs (EBER1) and qPCR was performed to detect the EBV-EBNA-1 gene region. Cohen's kappa index and the chi-square test were used to compare the methodologies and investigate correlations with the clinical-pathological data of the gastric adenocarcinoma patients. Most of the patients were men, and the average age was 60 years. The intestinal subtype cancer presented more aggressive stages with 90% of patients having a reactive FISH for EBV (EBV+), although the virus infection frequency in epithelial gastric tissue was only 1%. No positive association with clinicopathological features and EBV+ was found by FISH. Using qPCR analysis, the percentage of positive samples was lower (52.4%), and a positive association was found in samples from older patients (> 60 years). Interestingly, 71 qPCR-negative cases were detected by FISH in the presence of non-epithelial cells and in 10 qPCR-positive cases with no evidence of EBV according to FISH. The concordance between the two techniques was low, with only 57.6%. FISH is more informative for associating the gastric carcinoma with EBV positivity in tumor/epithelial cells; however, qPCR can provide relevant information regarding the progression and characteristics of neoplasia.
id IEC-2_707982411e6947c15f2d7b884c177325
oai_identifier_str oai:patua.iec.gov.br:iec/4495
network_acronym_str IEC-2
network_name_str Repositório Digital do Instituto Evandro Chagas (Patuá)
repository_id_str
spelling Costa, Igor BrasilSouza, Carolina Rosal Teixeira deCosta, Iran BarrosSantos, Liann Filiphe Pereira dosPaixão, Luana César FerrazPolaro, Alessandra AlvesMonteiro, Talita Antônia FurtadoBurbano, Rommel Mário Rodríguez2022-05-12T13:44:04Z2022-05-12T13:44:04Z2022COSTA, Igor Brasil et al. Detection of Epstein-Barr virus in gastric adenocarcinoma: qPCR and fish comparison. Medical Microbiology and Immunology, v. 211, n. 1, p. 29-36, Feb. 2022.1432-1831https://patua.iec.gov.br/handle/iec/449510.1007/s00430-021-00724-3EBV-associated gastric cancer accounts for about 10% of all gastric carcinomas worldwide. We aimed to verify the prevalence of EBV in gastric adenocarcinoma samples using FISH and qPCR and comparing the results obtained by both techniques. Gastric cancer samples from 191 cases were analyzed. The FISH assay was performed to detect small EBV RNAs (EBER1) and qPCR was performed to detect the EBV-EBNA-1 gene region. Cohen's kappa index and the chi-square test were used to compare the methodologies and investigate correlations with the clinical-pathological data of the gastric adenocarcinoma patients. Most of the patients were men, and the average age was 60 years. The intestinal subtype cancer presented more aggressive stages with 90% of patients having a reactive FISH for EBV (EBV+), although the virus infection frequency in epithelial gastric tissue was only 1%. No positive association with clinicopathological features and EBV+ was found by FISH. Using qPCR analysis, the percentage of positive samples was lower (52.4%), and a positive association was found in samples from older patients (> 60 years). Interestingly, 71 qPCR-negative cases were detected by FISH in the presence of non-epithelial cells and in 10 qPCR-positive cases with no evidence of EBV according to FISH. The concordance between the two techniques was low, with only 57.6%. FISH is more informative for associating the gastric carcinoma with EBV positivity in tumor/epithelial cells; however, qPCR can provide relevant information regarding the progression and characteristics of neoplasia.Scientifc initiation scholarships were paid by National Council for Scientifc and Technological Development (CNPq). Salaries were paid by Evandro Chagas Institute (IEC) and Federal University of Pará. Equipment and supplies were provided by the IECMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, BrasilFederal University of Pará. Institute of Biological Sciences. Laboratory of Human Cytogenetics. Belém, PA, BrazilMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, BrasilMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, BrasilMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, BrasilMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, BrasilMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, BrasilFederal University of Pará. Institute of Biological Sciences. Laboratory of Human Cytogenetics. Belém, PA , Brazil / Ophir Loyola Hospital. Molecular Biology Laboratory. Belém, PA, BrazilengSpringerDetection of Epstein-Barr virus in gastric adenocarcinoma: qPCR and fish comparisoninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleNeoplasias Gástricas / patologiaInfecções por Vírus Epstein-BarrAdenocarcinomainfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALDetection of Epstein-Barr virus in gastric adenocarcinoma: qPCR and fish comparison.pdfDetection of Epstein-Barr virus in gastric adenocarcinoma: qPCR and fish comparison.pdfapplication/pdf834416https://patua.iec.gov.br/bitstreams/58a1db09-393b-4fb3-b58c-d546281c7575/download297ce22cefb445661a63a13758fdd04fMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82182https://patua.iec.gov.br/bitstreams/3bdc8ef6-7c2c-44d6-8813-a22eed2c04c7/download11832eea31b16df8613079d742d61793MD52TEXTDetection of Epstein-Barr virus in gastric adenocarcinoma: qPCR and fish comparison.pdf.txtDetection of Epstein-Barr virus in gastric adenocarcinoma: qPCR and fish comparison.pdf.txtExtracted texttext/plain38459https://patua.iec.gov.br/bitstreams/284b020a-815e-44bc-a0aa-da9f9276cd92/download396faf59b2d2969d88ad464fbef686b3MD55THUMBNAILDetection of Epstein-Barr virus in gastric adenocarcinoma: qPCR and fish comparison.pdf.jpgDetection of Epstein-Barr virus in gastric adenocarcinoma: qPCR and fish comparison.pdf.jpgGenerated Thumbnailimage/jpeg5942https://patua.iec.gov.br/bitstreams/7853966c-9fd3-4746-981e-c774cb366740/download72495a1dbde266e47c0549a4b7f33ba6MD56iec/44952022-10-20 22:41:54.455oai:patua.iec.gov.br:iec/4495https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-20T22:41:54Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)falsePGI+TGljZW7Dp2EgZGUgRGlzdHJpYnVpw6fDo28gTsOjbyBFeGNsdXNpdmE8L2I+DQoNClRvZG8gZGVwb3NpdGFudGUgZGUgbWF0ZXJpYWwgbm8gUGF0dcOhIGRldmUgY29uY2VkZXIgYW8gSW5zdGl0dXRvIEV2YW5kcm8gQ2hhZ2FzIHVtYSBMaWNlbsOnYSBkZSBEaXN0cmlidWnDp8OjbyBOw6NvIEV4Y2x1c2l2YSBwYXJhIHRvcm5hciBlIG1hbnRlciBhY2Vzc8OtdmVpcyBvcyBzZXVzIGRvY3VtZW50b3MgZW0gZm9ybWF0byBkaWdpdGFsLiANCg0KQ29tIGEgY29uY2Vzc8OjbyBkZXNzYSBsaWNlbsOnYSBuw6NvIGV4Y2x1c2l2YSwgbyBkZXBvc2l0YW50ZSBjb250aW51YSBhIHJldGVyIHRvZG9zIG9zIGRpcmVpdG9zIGRlIGF1dG9yLg0KDQpBbyBhc3NpbmFyIGUgZW50cmVnYXIgZXN0YSBsaWNlbsOnYSB2b2PDqiAoYXV0b3Igb3UgZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzKQ0KDQphKSBEZWNsYXJhIHF1ZSBjb25oZWNlIGEgcG9sw610aWNhIGRlIGNvcHlyaWdodCBkYSBlZGl0b3JhIGRlIHNldSBkb2N1bWVudG8uDQoNCmIpIENvbmNlZGUgYW8gSW5zdGl0dXRvIEV2YW5kcm8gQ2hhZ2FzIG8gZGlyZWl0byBuw6NvIGV4Y2x1c2l2byBkZSBhcnF1aXZhciwgcmVwcm9kdXppciwgY29udmVydGVyIChjb21vIGRlZmluaWRvIGEgc2VndWlyKSwgY29tdW5pY2FyIGUvb3UgZGlzdHJpYnVpciBubyA8Yj5QYXR1w6E8L2I+LCBvIGRvY3VtZW50byBlbnRyZWd1ZSAoaW5jbHVpbmRvIG8gcmVzdW1vL2Fic3RyYWN0KSBlbSBmb3JtYXRvIGRpZ2l0YWwgb3UgcG9yIHF1YWxxdWVyIG91dHJvIG1laW8uDQoNCmMpIERlY2xhcmEgcXVlIGF1dG9yaXphIG8gSW5zdGl0dXRvIEV2YW5kcm8gQ2hhZ2FzIGEgYXJxdWl2YXIgbWFpcyBkZSB1bWEgY8OzcGlhIGRlc3NlIGRvY3VtZW50byBlIGNvbnZlcnTDqi1sbywgc2VtIGFsdGVyYXIgbyBzZXUgY29udGXDumRvLCBwYXJhIHF1YWxxdWVyIGZvcm1hdG8gZGUgZmljaGVpcm8sIG1laW8gb3Ugc3Vwb3J0ZSwgcGFyYSBlZmVpdG9zIGRlIHNlZ3VyYW7Dp2EsIHByZXNlcnZhw6fDo28gKGJhY2t1cCkgZSBhY2Vzc28uDQoNCmQpIERlY2xhcmEgcXVlIG8gZG9jdW1lbnRvIHN1Ym1ldGlkbyDDqSBvIHNldSB0cmFiYWxobyBvcmlnaW5hbCBlIHF1ZSBkZXTDqW0gbyBkaXJlaXRvIGRlIGNvbmNlZGVyIGEgdGVyY2Vpcm9zIG9zIGRpcmVpdG9zIGNvbnRpZG9zIG5lc3RhIGxpY2Vuw6dhLiBEZWNsYXJhIHRhbWLDqW0gcXVlIGEgZW50cmVnYSBkbyBkb2N1bWVudG8gbsOjbyBpbmZyaW5nZSBvcyBkaXJlaXRvcyBkZSBxdWFscXVlciBvdXRyYSBwZXNzb2Egb3UgZW50aWRhZGUuDQoNCmUpIERlY2xhcmEgcXVlLCBubyBjYXNvIGRvIGRvY3VtZW50byBzdWJtZXRpZG8gY29udGVyIG1hdGVyaWFsIGRvIHF1YWwgbsOjbyBkZXTDqW0gb3MgZGlyZWl0b3MgZGUgYXV0b3IsIG9idGV2ZSBhIGF1dG9yaXphw6fDo28gaXJyZXN0cml0YSBkbyByZXNwZWN0aXZvIGRldGVudG9yIGRlc3NlcyBkaXJlaXRvcywgcGFyYSBjZWRlciBhbyBJbnN0aXR1dG8gRXZhbmRybyBDaGFnYXMgb3MgZGlyZWl0b3MgcmVxdWVyaWRvcyBwb3IgZXN0YSBMaWNlbsOnYSBlIGF1dG9yaXphciBhIHV0aWxpesOhLWxvcyBsZWdhbG1lbnRlLiBEZWNsYXJhIHRhbWLDqW0gcXVlIGVzc2UgbWF0ZXJpYWwgY3Vqb3MgZGlyZWl0b3Mgc8OjbyBkZSB0ZXJjZWlyb3MgZXN0w6EgY2xhcmFtZW50ZSBpZGVudGlmaWNhZG8gZSByZWNvbmhlY2lkbyBubyB0ZXh0byBvdSBjb250ZcO6ZG8gZG8gZG9jdW1lbnRvIGVudHJlZ3VlLg0KDQpmKSBTRSBPIERPQ1VNRU5UTyBFTlRSRUdVRSBGT1IgQkFTRUFETyBFTSBUUkFCQUxITyBGSU5BTkNJQURPIE9VIEFQT0lBRE8gUE9SIE9VVFJBIElOU1RJVFVJw4fDg08gUVVFIE7Dg08gTyBJTlNUSVRVVE8gRVZBTkRSTyBDSEFHQVMsIERFQ0xBUkEgUVVFIENVTVBSSVUgUVVBSVNRVUVSIE9CUklHQcOHw5VFUyBFWElHSURBUyBQRUxPIFJFU1BFQ1RJVk8gQ09OVFJBVE8gT1UgQUNPUkRPLg0KDQpPIEluc3RpdHV0byBFdmFuZHJvIENoYWdhcyBpZGVudGlmaWNhcsOhIGNsYXJhbWVudGUgbyhzKSBub21lKHMpIGRvKHMpIGF1dG9yKGVzKSBkb3MgZGlyZWl0b3MgZG8gZG9jdW1lbnRvIGVudHJlZ3VlIGUgbsOjbyBmYXLDoSBxdWFscXVlciBhbHRlcmHDp8OjbyBhbMOpbSBkbyBwcmV2aXN0byBuYSBhbMOtbmVhIGIpLg==
dc.title.pt_BR.fl_str_mv Detection of Epstein-Barr virus in gastric adenocarcinoma: qPCR and fish comparison
title Detection of Epstein-Barr virus in gastric adenocarcinoma: qPCR and fish comparison
spellingShingle Detection of Epstein-Barr virus in gastric adenocarcinoma: qPCR and fish comparison
Costa, Igor Brasil
Neoplasias Gástricas / patologia
Infecções por Vírus Epstein-Barr
Adenocarcinoma
title_short Detection of Epstein-Barr virus in gastric adenocarcinoma: qPCR and fish comparison
title_full Detection of Epstein-Barr virus in gastric adenocarcinoma: qPCR and fish comparison
title_fullStr Detection of Epstein-Barr virus in gastric adenocarcinoma: qPCR and fish comparison
title_full_unstemmed Detection of Epstein-Barr virus in gastric adenocarcinoma: qPCR and fish comparison
title_sort Detection of Epstein-Barr virus in gastric adenocarcinoma: qPCR and fish comparison
author Costa, Igor Brasil
author_facet Costa, Igor Brasil
Souza, Carolina Rosal Teixeira de
Costa, Iran Barros
Santos, Liann Filiphe Pereira dos
Paixão, Luana César Ferraz
Polaro, Alessandra Alves
Monteiro, Talita Antônia Furtado
Burbano, Rommel Mário Rodríguez
author_role author
author2 Souza, Carolina Rosal Teixeira de
Costa, Iran Barros
Santos, Liann Filiphe Pereira dos
Paixão, Luana César Ferraz
Polaro, Alessandra Alves
Monteiro, Talita Antônia Furtado
Burbano, Rommel Mário Rodríguez
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Costa, Igor Brasil
Souza, Carolina Rosal Teixeira de
Costa, Iran Barros
Santos, Liann Filiphe Pereira dos
Paixão, Luana César Ferraz
Polaro, Alessandra Alves
Monteiro, Talita Antônia Furtado
Burbano, Rommel Mário Rodríguez
dc.subject.decsPrimary.pt_BR.fl_str_mv Neoplasias Gástricas / patologia
Infecções por Vírus Epstein-Barr
Adenocarcinoma
topic Neoplasias Gástricas / patologia
Infecções por Vírus Epstein-Barr
Adenocarcinoma
description EBV-associated gastric cancer accounts for about 10% of all gastric carcinomas worldwide. We aimed to verify the prevalence of EBV in gastric adenocarcinoma samples using FISH and qPCR and comparing the results obtained by both techniques. Gastric cancer samples from 191 cases were analyzed. The FISH assay was performed to detect small EBV RNAs (EBER1) and qPCR was performed to detect the EBV-EBNA-1 gene region. Cohen's kappa index and the chi-square test were used to compare the methodologies and investigate correlations with the clinical-pathological data of the gastric adenocarcinoma patients. Most of the patients were men, and the average age was 60 years. The intestinal subtype cancer presented more aggressive stages with 90% of patients having a reactive FISH for EBV (EBV+), although the virus infection frequency in epithelial gastric tissue was only 1%. No positive association with clinicopathological features and EBV+ was found by FISH. Using qPCR analysis, the percentage of positive samples was lower (52.4%), and a positive association was found in samples from older patients (> 60 years). Interestingly, 71 qPCR-negative cases were detected by FISH in the presence of non-epithelial cells and in 10 qPCR-positive cases with no evidence of EBV according to FISH. The concordance between the two techniques was low, with only 57.6%. FISH is more informative for associating the gastric carcinoma with EBV positivity in tumor/epithelial cells; however, qPCR can provide relevant information regarding the progression and characteristics of neoplasia.
publishDate 2022
dc.date.accessioned.fl_str_mv 2022-05-12T13:44:04Z
dc.date.available.fl_str_mv 2022-05-12T13:44:04Z
dc.date.issued.fl_str_mv 2022
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv COSTA, Igor Brasil et al. Detection of Epstein-Barr virus in gastric adenocarcinoma: qPCR and fish comparison. Medical Microbiology and Immunology, v. 211, n. 1, p. 29-36, Feb. 2022.
dc.identifier.uri.fl_str_mv https://patua.iec.gov.br/handle/iec/4495
dc.identifier.issn.-.fl_str_mv 1432-1831
dc.identifier.doi.-.fl_str_mv 10.1007/s00430-021-00724-3
identifier_str_mv COSTA, Igor Brasil et al. Detection of Epstein-Barr virus in gastric adenocarcinoma: qPCR and fish comparison. Medical Microbiology and Immunology, v. 211, n. 1, p. 29-36, Feb. 2022.
1432-1831
10.1007/s00430-021-00724-3
url https://patua.iec.gov.br/handle/iec/4495
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositório Digital do Instituto Evandro Chagas (Patuá)
instname:Instituto Evandro Chagas (IEC)
instacron:IEC
instname_str Instituto Evandro Chagas (IEC)
instacron_str IEC
institution IEC
reponame_str Repositório Digital do Instituto Evandro Chagas (Patuá)
collection Repositório Digital do Instituto Evandro Chagas (Patuá)
bitstream.url.fl_str_mv https://patua.iec.gov.br/bitstreams/58a1db09-393b-4fb3-b58c-d546281c7575/download
https://patua.iec.gov.br/bitstreams/3bdc8ef6-7c2c-44d6-8813-a22eed2c04c7/download
https://patua.iec.gov.br/bitstreams/284b020a-815e-44bc-a0aa-da9f9276cd92/download
https://patua.iec.gov.br/bitstreams/7853966c-9fd3-4746-981e-c774cb366740/download
bitstream.checksum.fl_str_mv 297ce22cefb445661a63a13758fdd04f
11832eea31b16df8613079d742d61793
396faf59b2d2969d88ad464fbef686b3
72495a1dbde266e47c0549a4b7f33ba6
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
repository.name.fl_str_mv Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)
repository.mail.fl_str_mv clariceneta@iec.gov.br || Biblioteca@iec.gov.br
_version_ 1809190049251590144