Further evidence associating IgG1, but not IgG2, with susceptibility to canine visceral leishmaniasis caused by Leishmania (L.) infantum chagasi-infection
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/2858 https://doi.org/10.1051/parasite/2017039 |
Resumo: | We present here a cross-sectional study analyzing the IgG1 and IgG2 immune responses to natural canine Leishmania (L.) infantum chagasi-infection and their relationships with delayed-type hypersensitivity (DTH) in 50 mongrel dogs with previous positive serodiagnoses (IFAT-IgG) (56% with subclinical status [= apparently healthy] and 44% clinically sick), living in endemic areas for visceral leishmaniasis in the Brazilian Amazon. IgG1 and IgG2 responses were measured using commercial polyclonal antibodies in ELISA, while DTH was elicited by intradermal skin test using cultured promastigotes L. (L.) i. chagasi-antigen. Data analyses used Chi-square and Pearson's r coefficient (95% confidence interval). Regarding DTH and the clinical statuses of dogs, it was noted that 100% of the animals showing positive DTH (n = 8) were from the subclinical group, while 100% showing negative DTH were from the clinically sick group; higher IgG2 than IgG1 responses were observed in both clinical groups. However, when this comparison was made between the subclinical and sick groups, higher IgG1 responses were noted in the dogs from the sick rather than the subclinical group, while no differences were noted between the IgG2 responses in the dogs from both clinical groups. Additionally, we found lower IgG1 responses in dogs from the subclinical group showing positive DTH than in the dogs from the subclinical or sick groups with negative DTH; no differences were found between the IgG2 responses of these two clinical groups. These findings suggest that the IgG1, but not the IgG2, response is associated with susceptibility to canine visceral leishmaniasis (CVL). |
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Lima, Luciana Vieira do RêgoCarneiro, Liliane AlmeidaCampos, Marliane BatistaSantos, Thiago Vasconcelos dosRamos, Patricia KarlaLaurenti, Márcia DalastraTeixeira, Claudio Eduardo CSilveira, Fernando Tobias2017-11-20T18:25:43Z2017-11-20T18:25:43Z2017LIMA, Luciana Vieira do Rêgo et al. Further evidence associating IgG1, but not IgG2, with susceptibility to canine visceral leishmaniasis caused by Leishmania (L.) infantum chagasi-infection. Parasite, v. 24, n. 37, p. 1-11, Oct. 2017. DOI: https://doi.org/10.1051/parasite/2017039. Disponível em: https://www.parasite-journal.org/articles/parasite/pdf/2017/01/parasite170041.pdf. Acesso em: 20 nov. 20171252-607Xhttps://patua.iec.gov.br/handle/iec/2858https://doi.org/10.1051/parasite/2017039We present here a cross-sectional study analyzing the IgG1 and IgG2 immune responses to natural canine Leishmania (L.) infantum chagasi-infection and their relationships with delayed-type hypersensitivity (DTH) in 50 mongrel dogs with previous positive serodiagnoses (IFAT-IgG) (56% with subclinical status [= apparently healthy] and 44% clinically sick), living in endemic areas for visceral leishmaniasis in the Brazilian Amazon. IgG1 and IgG2 responses were measured using commercial polyclonal antibodies in ELISA, while DTH was elicited by intradermal skin test using cultured promastigotes L. (L.) i. chagasi-antigen. Data analyses used Chi-square and Pearson's r coefficient (95% confidence interval). Regarding DTH and the clinical statuses of dogs, it was noted that 100% of the animals showing positive DTH (n = 8) were from the subclinical group, while 100% showing negative DTH were from the clinically sick group; higher IgG2 than IgG1 responses were observed in both clinical groups. However, when this comparison was made between the subclinical and sick groups, higher IgG1 responses were noted in the dogs from the sick rather than the subclinical group, while no differences were noted between the IgG2 responses in the dogs from both clinical groups. Additionally, we found lower IgG1 responses in dogs from the subclinical group showing positive DTH than in the dogs from the subclinical or sick groups with negative DTH; no differences were found between the IgG2 responses of these two clinical groups. These findings suggest that the IgG1, but not the IgG2, response is associated with susceptibility to canine visceral leishmaniasis (CVL).This research was supported by the Evandro Chagas Institute (Ministry of Health, Brazil) and Nucleus of Tropical Medicine (Federal University of Pará State, Brazil).Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Centro Nacional de Primatas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.University São Paulo. Medical School of São Paulo. Pathology Department. São Paulo, SP, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Federal University of Pará. Tropical Medicine Nucleus. Belém, PA, Brazil.engEDP OpenFurther evidence associating IgG1, but not IgG2, with susceptibility to canine visceral leishmaniasis caused by Leishmania (L.) infantum chagasi-infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLeishmania infantum / parasitologiaLeishmania infantum / patogenicidadeLeishmaniose Visceral / patogenicidadeLeishmaniose Visceral / parasitologiaCães / parasitologiaImunoglobulina G / imunologiaEnsaio de Imunoadsorção Enzimática / métodosinfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECLICENSElicense.txtlicense.txttext/plain; charset=utf-871https://patua.iec.gov.br/bitstreams/00b90aea-7c2a-4dce-bbe5-792138c293cd/download52f1732ea66fbd1123abe39f5373b797MD52ORIGINALFurther evidence associating IgG1, but not IgG2, with susceptibility to canine visceral leishmaniasis caused by Leishmania (L.) infantum chagasi-infection.pdfFurther evidence associating IgG1, but not IgG2, with susceptibility to canine visceral leishmaniasis caused by Leishmania (L.) infantum chagasi-infection.pdfapplication/pdf1510174https://patua.iec.gov.br/bitstreams/fac11025-f105-4101-ae89-e3f9f440b7e2/downloade5de385cffaa51d961bb61126dadd278MD53TEXTFurther evidence associating IgG1, but not IgG2, with susceptibility to canine visceral leishmaniasis caused by Leishmania (L.) infantum chagasi-infection.pdf.txtFurther evidence associating IgG1, but not IgG2, with susceptibility to canine visceral leishmaniasis caused by Leishmania (L.) infantum chagasi-infection.pdf.txtExtracted texttext/plain49470https://patua.iec.gov.br/bitstreams/035a58f2-e03e-4692-8c2c-5e0e4d0c319c/download5d3801e5023598d793b1198a8f4edb0eMD56THUMBNAILFurther evidence associating IgG1, but not IgG2, with susceptibility to canine visceral leishmaniasis caused by Leishmania (L.) infantum chagasi-infection.pdf.jpgFurther evidence associating IgG1, but not IgG2, with susceptibility to canine visceral leishmaniasis caused by Leishmania (L.) infantum chagasi-infection.pdf.jpgGenerated Thumbnailimage/jpeg5637https://patua.iec.gov.br/bitstreams/2e515af6-9e52-4dde-a998-b0e4ada0473b/downloadf390337516052511dca4d17ce8314819MD57iec/28582023-04-18 12:09:57.935oai:patua.iec.gov.br:iec/2858https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2023-04-18T12:09:57Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)falseVG9kb3Mgb3MgZG9jdW1lbnRvcyBkZXNzYSBjb2xlw6fDo28gc2VndWVtIGEgTGljZW7Dp2EgQ3JlYXRpdmUgY29tbW9ucy4= |
dc.title.pt_BR.fl_str_mv |
Further evidence associating IgG1, but not IgG2, with susceptibility to canine visceral leishmaniasis caused by Leishmania (L.) infantum chagasi-infection |
title |
Further evidence associating IgG1, but not IgG2, with susceptibility to canine visceral leishmaniasis caused by Leishmania (L.) infantum chagasi-infection |
spellingShingle |
Further evidence associating IgG1, but not IgG2, with susceptibility to canine visceral leishmaniasis caused by Leishmania (L.) infantum chagasi-infection Lima, Luciana Vieira do Rêgo Leishmania infantum / parasitologia Leishmania infantum / patogenicidade Leishmaniose Visceral / patogenicidade Leishmaniose Visceral / parasitologia Cães / parasitologia Imunoglobulina G / imunologia Ensaio de Imunoadsorção Enzimática / métodos |
title_short |
Further evidence associating IgG1, but not IgG2, with susceptibility to canine visceral leishmaniasis caused by Leishmania (L.) infantum chagasi-infection |
title_full |
Further evidence associating IgG1, but not IgG2, with susceptibility to canine visceral leishmaniasis caused by Leishmania (L.) infantum chagasi-infection |
title_fullStr |
Further evidence associating IgG1, but not IgG2, with susceptibility to canine visceral leishmaniasis caused by Leishmania (L.) infantum chagasi-infection |
title_full_unstemmed |
Further evidence associating IgG1, but not IgG2, with susceptibility to canine visceral leishmaniasis caused by Leishmania (L.) infantum chagasi-infection |
title_sort |
Further evidence associating IgG1, but not IgG2, with susceptibility to canine visceral leishmaniasis caused by Leishmania (L.) infantum chagasi-infection |
author |
Lima, Luciana Vieira do Rêgo |
author_facet |
Lima, Luciana Vieira do Rêgo Carneiro, Liliane Almeida Campos, Marliane Batista Santos, Thiago Vasconcelos dos Ramos, Patricia Karla Laurenti, Márcia Dalastra Teixeira, Claudio Eduardo C Silveira, Fernando Tobias |
author_role |
author |
author2 |
Carneiro, Liliane Almeida Campos, Marliane Batista Santos, Thiago Vasconcelos dos Ramos, Patricia Karla Laurenti, Márcia Dalastra Teixeira, Claudio Eduardo C Silveira, Fernando Tobias |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Lima, Luciana Vieira do Rêgo Carneiro, Liliane Almeida Campos, Marliane Batista Santos, Thiago Vasconcelos dos Ramos, Patricia Karla Laurenti, Márcia Dalastra Teixeira, Claudio Eduardo C Silveira, Fernando Tobias |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Leishmania infantum / parasitologia Leishmania infantum / patogenicidade Leishmaniose Visceral / patogenicidade Leishmaniose Visceral / parasitologia Cães / parasitologia Imunoglobulina G / imunologia Ensaio de Imunoadsorção Enzimática / métodos |
topic |
Leishmania infantum / parasitologia Leishmania infantum / patogenicidade Leishmaniose Visceral / patogenicidade Leishmaniose Visceral / parasitologia Cães / parasitologia Imunoglobulina G / imunologia Ensaio de Imunoadsorção Enzimática / métodos |
description |
We present here a cross-sectional study analyzing the IgG1 and IgG2 immune responses to natural canine Leishmania (L.) infantum chagasi-infection and their relationships with delayed-type hypersensitivity (DTH) in 50 mongrel dogs with previous positive serodiagnoses (IFAT-IgG) (56% with subclinical status [= apparently healthy] and 44% clinically sick), living in endemic areas for visceral leishmaniasis in the Brazilian Amazon. IgG1 and IgG2 responses were measured using commercial polyclonal antibodies in ELISA, while DTH was elicited by intradermal skin test using cultured promastigotes L. (L.) i. chagasi-antigen. Data analyses used Chi-square and Pearson's r coefficient (95% confidence interval). Regarding DTH and the clinical statuses of dogs, it was noted that 100% of the animals showing positive DTH (n = 8) were from the subclinical group, while 100% showing negative DTH were from the clinically sick group; higher IgG2 than IgG1 responses were observed in both clinical groups. However, when this comparison was made between the subclinical and sick groups, higher IgG1 responses were noted in the dogs from the sick rather than the subclinical group, while no differences were noted between the IgG2 responses in the dogs from both clinical groups. Additionally, we found lower IgG1 responses in dogs from the subclinical group showing positive DTH than in the dogs from the subclinical or sick groups with negative DTH; no differences were found between the IgG2 responses of these two clinical groups. These findings suggest that the IgG1, but not the IgG2, response is associated with susceptibility to canine visceral leishmaniasis (CVL). |
publishDate |
2017 |
dc.date.accessioned.fl_str_mv |
2017-11-20T18:25:43Z |
dc.date.available.fl_str_mv |
2017-11-20T18:25:43Z |
dc.date.issued.fl_str_mv |
2017 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
LIMA, Luciana Vieira do Rêgo et al. Further evidence associating IgG1, but not IgG2, with susceptibility to canine visceral leishmaniasis caused by Leishmania (L.) infantum chagasi-infection. Parasite, v. 24, n. 37, p. 1-11, Oct. 2017. DOI: https://doi.org/10.1051/parasite/2017039. Disponível em: https://www.parasite-journal.org/articles/parasite/pdf/2017/01/parasite170041.pdf. Acesso em: 20 nov. 2017 |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/2858 |
dc.identifier.issn.-.fl_str_mv |
1252-607X |
dc.identifier.doi.-.fl_str_mv |
https://doi.org/10.1051/parasite/2017039 |
identifier_str_mv |
LIMA, Luciana Vieira do Rêgo et al. Further evidence associating IgG1, but not IgG2, with susceptibility to canine visceral leishmaniasis caused by Leishmania (L.) infantum chagasi-infection. Parasite, v. 24, n. 37, p. 1-11, Oct. 2017. DOI: https://doi.org/10.1051/parasite/2017039. Disponível em: https://www.parasite-journal.org/articles/parasite/pdf/2017/01/parasite170041.pdf. Acesso em: 20 nov. 2017 1252-607X |
url |
https://patua.iec.gov.br/handle/iec/2858 https://doi.org/10.1051/parasite/2017039 |
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eng |
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