Cytotoxic effect of cipó-pucá (Cissus sicyoides L.) supercritical extract on human red blood cells and as anti-inflammatory in spinal cord injury in adult rats

Detalhes bibliográficos
Autor(a) principal: Salazar, Marielba de los Ángeles Rodríguez
Data de Publicação: 2021
Outros Autores: Urbina, Glides Rafael Olivo, Cunha, Vânia Maria Borges, Bezerra, Fernanda Wariss Figueiredo, Dias, Michelle Nerissa Coelho, Santos, Ijair Rogério, Teixeira, Bruno José Brito, Costa, Wanessa Almeida, Leal, Walace Gomes, Souza, Jesus Nazareno Silva e, Silva, Silvia Helena Marques, Carvalho Junior, Raul Nunes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
Texto Completo: https://patua.iec.gov.br/handle/iec/4238
Resumo: Cissus sicyoides L. is a plant that has great anti-inflammatory activity, due to the presence of phenolic compounds. The objective of this work was to identify such substances by HPLC and to evaluate the cytotoxic effect on human red blood cells, of the C. sicyoides extract obtained by supercritical extraction, using CO2 and EtOH as co-solvent, in comparison to the extract obtained by conventional extraction (Soxhlet), as well as, to evaluate its anti-inflammatory effect on spinal cord injury (SCI) in adult rats. In HPLC analysis, the UV/Visible spectral data revealed compounds with absorption bands in wavelengths characteristic of glycosylated flavonoids. The supercritical extract did not show cytotoxic effect, as there was no rupture of red blood cell membranes. The in vivo test demonstrated reduction in cell concentration in the lesion surrounding area in treated animals, suggesting an anti-inflammatory effect of the supercritical extract on the SCI model.
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spelling Salazar, Marielba de los Ángeles RodríguezUrbina, Glides Rafael OlivoCunha, Vânia Maria BorgesBezerra, Fernanda Wariss FigueiredoDias, Michelle Nerissa CoelhoSantos, Ijair RogérioTeixeira, Bruno José BritoCosta, Wanessa AlmeidaLeal, Walace GomesSouza, Jesus Nazareno Silva eSilva, Silvia Helena MarquesCarvalho Junior, Raul Nunes2021-02-03T17:10:47Z2021-02-03T17:10:47Z2021SALAZAR, Marielba de los Ángeles Rodríguez et al. Cytotoxic effect of cipó-pucá (Cissus sicyoides L.) supercritical extract on human red blood cells and as anti-inflammatory in spinal cord injury in adult rats. The Journal of Supercritical Fluids, v. 169, n. xx, p. xx, Feb. 2021.0896-8446https://patua.iec.gov.br/handle/iec/423810.1016/j.supflu.2020.105105Cissus sicyoides L. is a plant that has great anti-inflammatory activity, due to the presence of phenolic compounds. The objective of this work was to identify such substances by HPLC and to evaluate the cytotoxic effect on human red blood cells, of the C. sicyoides extract obtained by supercritical extraction, using CO2 and EtOH as co-solvent, in comparison to the extract obtained by conventional extraction (Soxhlet), as well as, to evaluate its anti-inflammatory effect on spinal cord injury (SCI) in adult rats. In HPLC analysis, the UV/Visible spectral data revealed compounds with absorption bands in wavelengths characteristic of glycosylated flavonoids. The supercritical extract did not show cytotoxic effect, as there was no rupture of red blood cell membranes. The in vivo test demonstrated reduction in cell concentration in the lesion surrounding area in treated animals, suggesting an anti-inflammatory effect of the supercritical extract on the SCI model.Federal University of Pará. Program of Post-Graduation in Food Science and Technology. Extraction Laboratory. Belém, PA, Brazil.Federal University of Pará. Program of Post-Graduation in Food Science and Technology. Extraction Laboratory. Belém, PA, Brazil.Federal University of Pará. Program of Post-Graduation in Food Science and Technology. Extraction Laboratory. Belém, PA, Brazil.Federal University of Pará. Program of Post-Graduation in Food Science and Technology. Extraction Laboratory. Belém, PA, Brazil.Federal University of Pará. Institute of Biological Sciences. Laboratory of Neuroprotection and Experimental Neurorregeneration. Belém, PA, Brazil.Federal University of Pará. Institute of Biological Sciences. Laboratory of Neuroprotection and Experimental Neurorregeneration. Belém, PA, Brazil.Federal University of Pará. Center for Valorization of Amazonian Bioactive Compounds. Belém, PA, Brazil.Federal University of Pará. Program of Post-Graduation in Food Science and Technology. Extraction Laboratory. Belém, PA, Brazil.Federal University of Pará. Institute of Biological Sciences. Laboratory of Neuroprotection and Experimental Neurorregeneration. Belém, PA, Brazil.Federal University of Pará. Center for Valorization of Amazonian Bioactive Compounds. Belém, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Federal University of Pará. Program of Post-Graduation in Food Science and Technology. Extraction Laboratory. Belém, PA, Brazil.engElsevierCytotoxic effect of cipó-pucá (Cissus sicyoides L.) supercritical extract on human red blood cells and as anti-inflammatory in spinal cord injury in adult ratsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePlantas Medicinais / químicaCissus / análiseCissus / químicaCompostos Fenólicos / análiseExtratos Vegetais / uso terapêuticoCromatografia Líquida de Alta Pressão / métodosinfo:eu-repo/semantics/embargoedAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALCytotoxic effect of cipó-pucá (Cissus sicyoides L.) supercritical extract on human red blood cells and as anti-inflammatory in spinal cord injury in adult rats.pdfCytotoxic effect of cipó-pucá (Cissus sicyoides L.) supercritical extract on human red blood cells and as anti-inflammatory in spinal cord injury in adult rats.pdfapplication/pdf551083https://patua.iec.gov.br/bitstreams/569f099d-43f9-426c-a1e1-a8f80ae97f6b/downloadc9a9c128e29cac82a5d7fdf3f4e6da73MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82182https://patua.iec.gov.br/bitstreams/4d8ae0c1-2822-472c-ba23-f174270ef5fa/download11832eea31b16df8613079d742d61793MD52TEXTCytotoxic effect of cipó-pucá (Cissus sicyoides L.) supercritical extract on human red blood cells and as anti-inflammatory in spinal cord injury in adult rats.pdf.txtCytotoxic effect of cipó-pucá (Cissus sicyoides L.) supercritical extract on human red blood cells and as anti-inflammatory in spinal cord injury in adult rats.pdf.txtExtracted texttext/plain2https://patua.iec.gov.br/bitstreams/9d3ff88f-56cd-4b26-b435-dd221791ce6c/downloade1c06d85ae7b8b032bef47e42e4c08f9MD55THUMBNAILCytotoxic effect of cipó-pucá (Cissus sicyoides L.) supercritical extract on human red blood cells and as anti-inflammatory in spinal cord injury in adult rats.pdf.jpgCytotoxic effect of cipó-pucá (Cissus sicyoides L.) supercritical extract on human red blood cells and as anti-inflammatory in spinal cord injury in adult rats.pdf.jpgGenerated Thumbnailimage/jpeg3095https://patua.iec.gov.br/bitstreams/3c58d482-f77b-4417-94ce-c46ecf3567c2/download71859d578212107f7f8c49a4ce09d9eeMD56iec/42382022-10-20 21:11:42.861oai:patua.iec.gov.br:iec/4238https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-20T21:11:42Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)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
dc.title.pt_BR.fl_str_mv Cytotoxic effect of cipó-pucá (Cissus sicyoides L.) supercritical extract on human red blood cells and as anti-inflammatory in spinal cord injury in adult rats
title Cytotoxic effect of cipó-pucá (Cissus sicyoides L.) supercritical extract on human red blood cells and as anti-inflammatory in spinal cord injury in adult rats
spellingShingle Cytotoxic effect of cipó-pucá (Cissus sicyoides L.) supercritical extract on human red blood cells and as anti-inflammatory in spinal cord injury in adult rats
Salazar, Marielba de los Ángeles Rodríguez
Plantas Medicinais / química
Cissus / análise
Cissus / química
Compostos Fenólicos / análise
Extratos Vegetais / uso terapêutico
Cromatografia Líquida de Alta Pressão / métodos
title_short Cytotoxic effect of cipó-pucá (Cissus sicyoides L.) supercritical extract on human red blood cells and as anti-inflammatory in spinal cord injury in adult rats
title_full Cytotoxic effect of cipó-pucá (Cissus sicyoides L.) supercritical extract on human red blood cells and as anti-inflammatory in spinal cord injury in adult rats
title_fullStr Cytotoxic effect of cipó-pucá (Cissus sicyoides L.) supercritical extract on human red blood cells and as anti-inflammatory in spinal cord injury in adult rats
title_full_unstemmed Cytotoxic effect of cipó-pucá (Cissus sicyoides L.) supercritical extract on human red blood cells and as anti-inflammatory in spinal cord injury in adult rats
title_sort Cytotoxic effect of cipó-pucá (Cissus sicyoides L.) supercritical extract on human red blood cells and as anti-inflammatory in spinal cord injury in adult rats
author Salazar, Marielba de los Ángeles Rodríguez
author_facet Salazar, Marielba de los Ángeles Rodríguez
Urbina, Glides Rafael Olivo
Cunha, Vânia Maria Borges
Bezerra, Fernanda Wariss Figueiredo
Dias, Michelle Nerissa Coelho
Santos, Ijair Rogério
Teixeira, Bruno José Brito
Costa, Wanessa Almeida
Leal, Walace Gomes
Souza, Jesus Nazareno Silva e
Silva, Silvia Helena Marques
Carvalho Junior, Raul Nunes
author_role author
author2 Urbina, Glides Rafael Olivo
Cunha, Vânia Maria Borges
Bezerra, Fernanda Wariss Figueiredo
Dias, Michelle Nerissa Coelho
Santos, Ijair Rogério
Teixeira, Bruno José Brito
Costa, Wanessa Almeida
Leal, Walace Gomes
Souza, Jesus Nazareno Silva e
Silva, Silvia Helena Marques
Carvalho Junior, Raul Nunes
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Salazar, Marielba de los Ángeles Rodríguez
Urbina, Glides Rafael Olivo
Cunha, Vânia Maria Borges
Bezerra, Fernanda Wariss Figueiredo
Dias, Michelle Nerissa Coelho
Santos, Ijair Rogério
Teixeira, Bruno José Brito
Costa, Wanessa Almeida
Leal, Walace Gomes
Souza, Jesus Nazareno Silva e
Silva, Silvia Helena Marques
Carvalho Junior, Raul Nunes
dc.subject.decsPrimary.pt_BR.fl_str_mv Plantas Medicinais / química
Cissus / análise
Cissus / química
Compostos Fenólicos / análise
Extratos Vegetais / uso terapêutico
Cromatografia Líquida de Alta Pressão / métodos
topic Plantas Medicinais / química
Cissus / análise
Cissus / química
Compostos Fenólicos / análise
Extratos Vegetais / uso terapêutico
Cromatografia Líquida de Alta Pressão / métodos
description Cissus sicyoides L. is a plant that has great anti-inflammatory activity, due to the presence of phenolic compounds. The objective of this work was to identify such substances by HPLC and to evaluate the cytotoxic effect on human red blood cells, of the C. sicyoides extract obtained by supercritical extraction, using CO2 and EtOH as co-solvent, in comparison to the extract obtained by conventional extraction (Soxhlet), as well as, to evaluate its anti-inflammatory effect on spinal cord injury (SCI) in adult rats. In HPLC analysis, the UV/Visible spectral data revealed compounds with absorption bands in wavelengths characteristic of glycosylated flavonoids. The supercritical extract did not show cytotoxic effect, as there was no rupture of red blood cell membranes. The in vivo test demonstrated reduction in cell concentration in the lesion surrounding area in treated animals, suggesting an anti-inflammatory effect of the supercritical extract on the SCI model.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-02-03T17:10:47Z
dc.date.available.fl_str_mv 2021-02-03T17:10:47Z
dc.date.issued.fl_str_mv 2021
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SALAZAR, Marielba de los Ángeles Rodríguez et al. Cytotoxic effect of cipó-pucá (Cissus sicyoides L.) supercritical extract on human red blood cells and as anti-inflammatory in spinal cord injury in adult rats. The Journal of Supercritical Fluids, v. 169, n. xx, p. xx, Feb. 2021.
dc.identifier.uri.fl_str_mv https://patua.iec.gov.br/handle/iec/4238
dc.identifier.issn.-.fl_str_mv 0896-8446
dc.identifier.doi.-.fl_str_mv 10.1016/j.supflu.2020.105105
identifier_str_mv SALAZAR, Marielba de los Ángeles Rodríguez et al. Cytotoxic effect of cipó-pucá (Cissus sicyoides L.) supercritical extract on human red blood cells and as anti-inflammatory in spinal cord injury in adult rats. The Journal of Supercritical Fluids, v. 169, n. xx, p. xx, Feb. 2021.
0896-8446
10.1016/j.supflu.2020.105105
url https://patua.iec.gov.br/handle/iec/4238
dc.language.iso.fl_str_mv eng
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dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
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