Group A rotavirus gastroenteritis in orphaned Leopardus tigrinus and Leopardus pardalis (Carnivora: Felidae) in the Eastern Amazon
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/6827 |
Resumo: | Rotaviruses are highly infectious and transmitted by the fecal-oral route, representing a leading cause of diarrheal deaths in children in developing countries. Additionally, it causes significant economic impacts as an enteropathogen among domestic animals. This report presents clinical and diagnostic findings in two cases of rotavirus (RV) infection involving orphaned Leopardus tigrinus (Schreber, 1775) and Leopardus pardalis (Linnaeus, 1758), representing the first registered group A RV (RVA) infection in these species. Both felids were rescued in Pará State, Amazon Region, by the Brazilian Institute of Environment and Renewable Natural Resources and treated in an intensive care ward in a public Environmental Park of Belém City. After adaptation up to the 40th day of the quarantine period, these animals had no typical symptoms of acute, fulminant gastroenteritis. The collected samples were examined at the Virology Laboratory of Instituto Evandro Chagas. RVA antigen was detected in the blood and fecal samples of L. tigrinus by enzyme-linked immunosorbent assay and immunochromatography. RVA-positive samples were subjected to polyacrylamide gel electrophoresis and reverse transcriptase polymerase chain reaction for VP4 and VP7 genes using conventional set primers but with negative results. Stools were examined for ova and parasites yielding negative results and for intestinal endoparasites, yielding negative results. The animals died within a few days following a clinical exacerbation, therefore unresponsive to treatment. Necropsies and histopathological analysis were performed at the Laboratory of Veterinary Pathology of Universidade Federal Rural da Amazônia. Despite the pathologic findings typical of RV infection hemorrhagic enteritis was unusual if presumed etiology is considered. |
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Gabriel, Áurea MartinsPereira, Washington Luiz AssunçãoSilva, Klena Sarges Marruaz daKaiano, Jane Haruko LimaNobre, Akim Felipe SantosMascarenhas, Joana D'Arc PereiraLinhares, Alexandre da Costa2023-05-17T11:15:00Z2023-05-17T11:15:00Z2023GABRIEL, Áurea Martins et al. Group A rotavirus gastroenteritis in orphaned Leopardus tigrinus and Leopardus pardalis (Carnivora: Felidae) in the Eastern Amazon. Revista Pan-Amazônica de Saúde, v. 14, n. 202301030, p. 1-11. 2023. DOI: https://doi.org/10.5123/S2176-6223202301030. Disponível em: http://scielo.iec.gov.br/pdf/rpas/v14/2176-6223-rpas-14-e202301030.pdf2176-6223https://patua.iec.gov.br/handle/iec/682710.5123/S2176-6223202301030Rotaviruses are highly infectious and transmitted by the fecal-oral route, representing a leading cause of diarrheal deaths in children in developing countries. Additionally, it causes significant economic impacts as an enteropathogen among domestic animals. This report presents clinical and diagnostic findings in two cases of rotavirus (RV) infection involving orphaned Leopardus tigrinus (Schreber, 1775) and Leopardus pardalis (Linnaeus, 1758), representing the first registered group A RV (RVA) infection in these species. Both felids were rescued in Pará State, Amazon Region, by the Brazilian Institute of Environment and Renewable Natural Resources and treated in an intensive care ward in a public Environmental Park of Belém City. After adaptation up to the 40th day of the quarantine period, these animals had no typical symptoms of acute, fulminant gastroenteritis. The collected samples were examined at the Virology Laboratory of Instituto Evandro Chagas. RVA antigen was detected in the blood and fecal samples of L. tigrinus by enzyme-linked immunosorbent assay and immunochromatography. RVA-positive samples were subjected to polyacrylamide gel electrophoresis and reverse transcriptase polymerase chain reaction for VP4 and VP7 genes using conventional set primers but with negative results. Stools were examined for ova and parasites yielding negative results and for intestinal endoparasites, yielding negative results. The animals died within a few days following a clinical exacerbation, therefore unresponsive to treatment. Necropsies and histopathological analysis were performed at the Laboratory of Veterinary Pathology of Universidade Federal Rural da Amazônia. Despite the pathologic findings typical of RV infection hemorrhagic enteritis was unusual if presumed etiology is considered.Fundação para a Ciência e a Tecnologia (Portugal), through the research project GHTM– UID/Multi/04413/2013; and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brazil), for the Ph.D. scholarship of Áurea Martins Gabriel.Universidade Nova de Lisboa. Instituto de Higiene e Medicina Tropical. Lisboa, Portugal / Universidade Federal do Pará. Instituto de Ciências Biológicas. Programa de Pós-Graduação em Biologia de Agentes Infecciosos e Parasitários. Belém, PA, BrasilUniversidade Federal Rural da Amazônia. Laboratório de Patologia Animal. Belém, PA, Brasil.Ministério da Saúde. Secretaria de Ciência, Tecnologia, Inovação e Insumos Estratégicos. Instituto Evandro Chagas. Centro Nacional de Primatas. Ananindeua, PA, Brasil / Fundação Oswaldo Cruz. Instituto de Ciência e Tecnologia em Biomodelos. Rio de Janeiro, RJ, Brasil.Ministério da Saúde. Secretaria de Ciência, Tecnologia, Inovação e Insumos Estratégicos. Instituto Evandro Chagas. Laboratórios de Vírus Gastroentéricos. Ananindeua, PA, BrasilUniversidade Federal do Pará. Núcleo de Medicina Tropical. Laboratório de Biologia Molecular e Celular. Belém, PA, Brasil.Ministério da Saúde. Secretaria de Ciência, Tecnologia, Inovação e Insumos Estratégicos. Instituto Evandro Chagas. Laboratórios de Vírus Gastroentéricos. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Ciência, Tecnologia, Inovação e Insumos Estratégicos. Instituto Evandro Chagas. Laboratórios de Vírus Gastroentéricos. 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dc.title.pt_BR.fl_str_mv |
Group A rotavirus gastroenteritis in orphaned Leopardus tigrinus and Leopardus pardalis (Carnivora: Felidae) in the Eastern Amazon |
dc.title.alternative.pt_BR.fl_str_mv |
Gastroenterite por rotavírus do grupo A em Leopardus tigrinus e Leopardus pardalis (Carnivora: Felidae) órfãos na Amazônia Oriental |
title |
Group A rotavirus gastroenteritis in orphaned Leopardus tigrinus and Leopardus pardalis (Carnivora: Felidae) in the Eastern Amazon |
spellingShingle |
Group A rotavirus gastroenteritis in orphaned Leopardus tigrinus and Leopardus pardalis (Carnivora: Felidae) in the Eastern Amazon Gabriel, Áurea Martins Rotavirus / patogenicidade Infecções por Rotavirus Felidae / anatomia & histologia Zoonoses Virais |
title_short |
Group A rotavirus gastroenteritis in orphaned Leopardus tigrinus and Leopardus pardalis (Carnivora: Felidae) in the Eastern Amazon |
title_full |
Group A rotavirus gastroenteritis in orphaned Leopardus tigrinus and Leopardus pardalis (Carnivora: Felidae) in the Eastern Amazon |
title_fullStr |
Group A rotavirus gastroenteritis in orphaned Leopardus tigrinus and Leopardus pardalis (Carnivora: Felidae) in the Eastern Amazon |
title_full_unstemmed |
Group A rotavirus gastroenteritis in orphaned Leopardus tigrinus and Leopardus pardalis (Carnivora: Felidae) in the Eastern Amazon |
title_sort |
Group A rotavirus gastroenteritis in orphaned Leopardus tigrinus and Leopardus pardalis (Carnivora: Felidae) in the Eastern Amazon |
author |
Gabriel, Áurea Martins |
author_facet |
Gabriel, Áurea Martins Pereira, Washington Luiz Assunção Silva, Klena Sarges Marruaz da Kaiano, Jane Haruko Lima Nobre, Akim Felipe Santos Mascarenhas, Joana D'Arc Pereira Linhares, Alexandre da Costa |
author_role |
author |
author2 |
Pereira, Washington Luiz Assunção Silva, Klena Sarges Marruaz da Kaiano, Jane Haruko Lima Nobre, Akim Felipe Santos Mascarenhas, Joana D'Arc Pereira Linhares, Alexandre da Costa |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Gabriel, Áurea Martins Pereira, Washington Luiz Assunção Silva, Klena Sarges Marruaz da Kaiano, Jane Haruko Lima Nobre, Akim Felipe Santos Mascarenhas, Joana D'Arc Pereira Linhares, Alexandre da Costa |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Rotavirus / patogenicidade Infecções por Rotavirus Felidae / anatomia & histologia Zoonoses Virais |
topic |
Rotavirus / patogenicidade Infecções por Rotavirus Felidae / anatomia & histologia Zoonoses Virais |
description |
Rotaviruses are highly infectious and transmitted by the fecal-oral route, representing a leading cause of diarrheal deaths in children in developing countries. Additionally, it causes significant economic impacts as an enteropathogen among domestic animals. This report presents clinical and diagnostic findings in two cases of rotavirus (RV) infection involving orphaned Leopardus tigrinus (Schreber, 1775) and Leopardus pardalis (Linnaeus, 1758), representing the first registered group A RV (RVA) infection in these species. Both felids were rescued in Pará State, Amazon Region, by the Brazilian Institute of Environment and Renewable Natural Resources and treated in an intensive care ward in a public Environmental Park of Belém City. After adaptation up to the 40th day of the quarantine period, these animals had no typical symptoms of acute, fulminant gastroenteritis. The collected samples were examined at the Virology Laboratory of Instituto Evandro Chagas. RVA antigen was detected in the blood and fecal samples of L. tigrinus by enzyme-linked immunosorbent assay and immunochromatography. RVA-positive samples were subjected to polyacrylamide gel electrophoresis and reverse transcriptase polymerase chain reaction for VP4 and VP7 genes using conventional set primers but with negative results. Stools were examined for ova and parasites yielding negative results and for intestinal endoparasites, yielding negative results. The animals died within a few days following a clinical exacerbation, therefore unresponsive to treatment. Necropsies and histopathological analysis were performed at the Laboratory of Veterinary Pathology of Universidade Federal Rural da Amazônia. Despite the pathologic findings typical of RV infection hemorrhagic enteritis was unusual if presumed etiology is considered. |
publishDate |
2023 |
dc.date.accessioned.fl_str_mv |
2023-05-17T11:15:00Z |
dc.date.available.fl_str_mv |
2023-05-17T11:15:00Z |
dc.date.issued.fl_str_mv |
2023 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
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article |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
GABRIEL, Áurea Martins et al. Group A rotavirus gastroenteritis in orphaned Leopardus tigrinus and Leopardus pardalis (Carnivora: Felidae) in the Eastern Amazon. Revista Pan-Amazônica de Saúde, v. 14, n. 202301030, p. 1-11. 2023. DOI: https://doi.org/10.5123/S2176-6223202301030. Disponível em: http://scielo.iec.gov.br/pdf/rpas/v14/2176-6223-rpas-14-e202301030.pdf |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/6827 |
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2176-6223 |
dc.identifier.doi.pt_BR.fl_str_mv |
10.5123/S2176-6223202301030 |
identifier_str_mv |
GABRIEL, Áurea Martins et al. Group A rotavirus gastroenteritis in orphaned Leopardus tigrinus and Leopardus pardalis (Carnivora: Felidae) in the Eastern Amazon. Revista Pan-Amazônica de Saúde, v. 14, n. 202301030, p. 1-11. 2023. DOI: https://doi.org/10.5123/S2176-6223202301030. Disponível em: http://scielo.iec.gov.br/pdf/rpas/v14/2176-6223-rpas-14-e202301030.pdf 2176-6223 10.5123/S2176-6223202301030 |
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https://patua.iec.gov.br/handle/iec/6827 |
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