The use of the circulating cathodic antigen (Cca) urine cassette assay for the diagnosis and assessment of cure of schistosoma mansoni infections in an endemic

Detalhes bibliográficos
Autor(a) principal: Sousa, Sergei Rodrigo Magalhães de
Data de Publicação: 2020
Outros Autores: Nogueira, Joyce Favacho Cardoso, Dias, Isabelle Helena Lima, Fonseca, Álvaro Luan Santana, Favero, Vivian, Geiger, Stefan Michael, Enk, Martin Johannes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
Texto Completo: https://patua.iec.gov.br/handle/iec/4185
Resumo: INTRODUCTION: Schistosomiasis is a poverty-related disease that affects people in 78 countries worldwide. This study aimed to evaluate the point-of-care circulating cathodic antigen (POC-CCA) test performance using sensitive parasitological methods as a reference standard (RS) in individuals before and after treatment. METHODS: The RS was established by combining the results of 16 Kato-Katz slides and the Helmintex® method. Positivity rates of the POC-CCA test and Kato-Katz and Helmintex® methods were calculated before treatment and 30 days afterward. Furthermore, the sensitivity, specificity, accuracy, and kappa coefficient before treatment were determined by comparing the methods. The cure rate was defined 30 days after treatment. RESULTS: Among the 217 participants, the RS detected a total of 63 (29.0%) positive individuals. The POC-CCA test identified 79 (36.4%) infections. The evaluation of POC-CCA test performance in relation to the RS revealed a sensitivity of 61.9%, specificity of 74.0%, accuracy of 70.5%, and kappa coefficient of 0.33. Out of the 53 remaining participants after treatment, a total of 45 (81.1%) showed egg negative results, and 8 (18.9%) were egg positive according to the RS. A total of 5 (9.4%) egg-positive and 37 (69.8%) egg-negative individuals were positive by the POC-CCA test. CONCLUSIONS: Our data show that the POC-CCA test has potential as an auxiliary tool for the diagnosis of Schistosoma mansoni infection, yielding better results than 16 Kato-Katz slides from three different stool samples. However, the immunochromatographic test lacks sufficient specificity and sensitivity for verifying the cure rate after treatment.
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spelling Sousa, Sergei Rodrigo Magalhães deNogueira, Joyce Favacho CardosoDias, Isabelle Helena LimaFonseca, Álvaro Luan SantanaFavero, VivianGeiger, Stefan MichaelEnk, Martin Johannes2020-10-14T01:34:18Z2020-10-14T01:34:18Z2020SOUSA, Sergei Rodrigo Magalhães de et al. The use of the circulating cathodic antigen (Cca) urine cassette assay for the diagnosis and assessment of cure of schistosoma mansoni infections in an endemic. Revista da Sociedade Brasileira de Medicina Tropical, v. 53, e20190562, Sept. 2020.1678-9849https://patua.iec.gov.br/handle/iec/418510.1590/0037-8682-0562-2019INTRODUCTION: Schistosomiasis is a poverty-related disease that affects people in 78 countries worldwide. This study aimed to evaluate the point-of-care circulating cathodic antigen (POC-CCA) test performance using sensitive parasitological methods as a reference standard (RS) in individuals before and after treatment. METHODS: The RS was established by combining the results of 16 Kato-Katz slides and the Helmintex® method. Positivity rates of the POC-CCA test and Kato-Katz and Helmintex® methods were calculated before treatment and 30 days afterward. Furthermore, the sensitivity, specificity, accuracy, and kappa coefficient before treatment were determined by comparing the methods. The cure rate was defined 30 days after treatment. RESULTS: Among the 217 participants, the RS detected a total of 63 (29.0%) positive individuals. The POC-CCA test identified 79 (36.4%) infections. The evaluation of POC-CCA test performance in relation to the RS revealed a sensitivity of 61.9%, specificity of 74.0%, accuracy of 70.5%, and kappa coefficient of 0.33. Out of the 53 remaining participants after treatment, a total of 45 (81.1%) showed egg negative results, and 8 (18.9%) were egg positive according to the RS. A total of 5 (9.4%) egg-positive and 37 (69.8%) egg-negative individuals were positive by the POC-CCA test. CONCLUSIONS: Our data show that the POC-CCA test has potential as an auxiliary tool for the diagnosis of Schistosoma mansoni infection, yielding better results than 16 Kato-Katz slides from three different stool samples. However, the immunochromatographic test lacks sufficient specificity and sensitivity for verifying the cure rate after treatment.Universidade do Estado do Pará. Programa de Pós-Graduação Strictu Sensu em Biologia Parasitária na Amazônia. Belém, PA, Brasil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Parasitoses Intestinais, Esquistossomose e Malacologia. Ananindeua, PA, Brasil.Universidade do Estado do Pará. Programa de Pós-Graduação Strictu Sensu em Biologia Parasitária na Amazônia. Belém, PA, Brasil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Parasitoses Intestinais, Esquistossomose e Malacologia. Ananindeua, PA, Brasil.Universidade do Estado do Pará. Programa de Pós-Graduação Strictu Sensu em Biologia Parasitária na Amazônia. Belém, PA, Brasil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Parasitoses Intestinais, Esquistossomose e Malacologia. Ananindeua, PA, Brasil.Universidade do Estado do Pará. Programa de Pós-Graduação Strictu Sensu em Biologia Parasitária na Amazônia. Belém, PA, Brasil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Parasitoses Intestinais, Esquistossomose e Malacologia. Ananindeua, PA, Brasil.Pontifícia Universidade Católica do Rio Grande do Sul. Programa de Pós-Graduação em Medicina e Ciências da Saúde. Laboratório de Parasitologia Biomédica. Porto Alegre, RS, Brasil.Universidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, Brasil.Universidade do Estado do Pará. Programa de Pós-Graduação Strictu Sensu em Biologia Parasitária na Amazônia. Belém, PA, Brasil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Parasitoses Intestinais, Esquistossomose e Malacologia. 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charset=utf-82182https://patua.iec.gov.br/bitstreams/52fee4aa-3e0d-44c6-b65b-e7ab5c74ce99/download11832eea31b16df8613079d742d61793MD52TEXTThe use of the circulating cathodic antigen (Cca) urine cassette assay for the diagnosis and assessment of cure of schistosoma mansoni infections in an endemic.pdf.txtThe use of the circulating cathodic antigen (Cca) urine cassette assay for the diagnosis and assessment of cure of schistosoma mansoni infections in an endemic.pdf.txtExtracted texttext/plain35842https://patua.iec.gov.br/bitstreams/85d80297-a20c-42f7-99fa-9670d728816e/downloade98e4b1c8d618ccb8794cacc232b06e1MD55THUMBNAILThe use of the circulating cathodic antigen (Cca) urine cassette assay for the diagnosis and assessment of cure of schistosoma mansoni infections in an endemic.pdf.jpgThe use of the circulating cathodic antigen (Cca) urine cassette assay for the diagnosis and assessment of cure of schistosoma mansoni infections in an endemic.pdf.jpgGenerated Thumbnailimage/jpeg6614https://patua.iec.gov.br/bitstreams/5ea0b871-b287-45c1-a458-42df845d490f/downloadbb130d805da3f87f5698bd8cb7ee84b0MD56iec/41852023-03-07 16:53:01.76oai:patua.iec.gov.br:iec/4185https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2023-03-07T16:53:01Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)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
dc.title.pt_BR.fl_str_mv The use of the circulating cathodic antigen (Cca) urine cassette assay for the diagnosis and assessment of cure of schistosoma mansoni infections in an endemic
title The use of the circulating cathodic antigen (Cca) urine cassette assay for the diagnosis and assessment of cure of schistosoma mansoni infections in an endemic
spellingShingle The use of the circulating cathodic antigen (Cca) urine cassette assay for the diagnosis and assessment of cure of schistosoma mansoni infections in an endemic
Sousa, Sergei Rodrigo Magalhães de
Esquistossomose mansoni / parasitologia
Esquistossomose mansoni / diagnóstico
Testes Imediatos
Sensibilidade e Especificidade
Contagem de Ovos de Parasitas
Kato-Katz
Helmintex®
title_short The use of the circulating cathodic antigen (Cca) urine cassette assay for the diagnosis and assessment of cure of schistosoma mansoni infections in an endemic
title_full The use of the circulating cathodic antigen (Cca) urine cassette assay for the diagnosis and assessment of cure of schistosoma mansoni infections in an endemic
title_fullStr The use of the circulating cathodic antigen (Cca) urine cassette assay for the diagnosis and assessment of cure of schistosoma mansoni infections in an endemic
title_full_unstemmed The use of the circulating cathodic antigen (Cca) urine cassette assay for the diagnosis and assessment of cure of schistosoma mansoni infections in an endemic
title_sort The use of the circulating cathodic antigen (Cca) urine cassette assay for the diagnosis and assessment of cure of schistosoma mansoni infections in an endemic
author Sousa, Sergei Rodrigo Magalhães de
author_facet Sousa, Sergei Rodrigo Magalhães de
Nogueira, Joyce Favacho Cardoso
Dias, Isabelle Helena Lima
Fonseca, Álvaro Luan Santana
Favero, Vivian
Geiger, Stefan Michael
Enk, Martin Johannes
author_role author
author2 Nogueira, Joyce Favacho Cardoso
Dias, Isabelle Helena Lima
Fonseca, Álvaro Luan Santana
Favero, Vivian
Geiger, Stefan Michael
Enk, Martin Johannes
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Sousa, Sergei Rodrigo Magalhães de
Nogueira, Joyce Favacho Cardoso
Dias, Isabelle Helena Lima
Fonseca, Álvaro Luan Santana
Favero, Vivian
Geiger, Stefan Michael
Enk, Martin Johannes
dc.subject.decsPrimary.pt_BR.fl_str_mv Esquistossomose mansoni / parasitologia
Esquistossomose mansoni / diagnóstico
Testes Imediatos
Sensibilidade e Especificidade
Contagem de Ovos de Parasitas
Kato-Katz
Helmintex®
topic Esquistossomose mansoni / parasitologia
Esquistossomose mansoni / diagnóstico
Testes Imediatos
Sensibilidade e Especificidade
Contagem de Ovos de Parasitas
Kato-Katz
Helmintex®
description INTRODUCTION: Schistosomiasis is a poverty-related disease that affects people in 78 countries worldwide. This study aimed to evaluate the point-of-care circulating cathodic antigen (POC-CCA) test performance using sensitive parasitological methods as a reference standard (RS) in individuals before and after treatment. METHODS: The RS was established by combining the results of 16 Kato-Katz slides and the Helmintex® method. Positivity rates of the POC-CCA test and Kato-Katz and Helmintex® methods were calculated before treatment and 30 days afterward. Furthermore, the sensitivity, specificity, accuracy, and kappa coefficient before treatment were determined by comparing the methods. The cure rate was defined 30 days after treatment. RESULTS: Among the 217 participants, the RS detected a total of 63 (29.0%) positive individuals. The POC-CCA test identified 79 (36.4%) infections. The evaluation of POC-CCA test performance in relation to the RS revealed a sensitivity of 61.9%, specificity of 74.0%, accuracy of 70.5%, and kappa coefficient of 0.33. Out of the 53 remaining participants after treatment, a total of 45 (81.1%) showed egg negative results, and 8 (18.9%) were egg positive according to the RS. A total of 5 (9.4%) egg-positive and 37 (69.8%) egg-negative individuals were positive by the POC-CCA test. CONCLUSIONS: Our data show that the POC-CCA test has potential as an auxiliary tool for the diagnosis of Schistosoma mansoni infection, yielding better results than 16 Kato-Katz slides from three different stool samples. However, the immunochromatographic test lacks sufficient specificity and sensitivity for verifying the cure rate after treatment.
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-10-14T01:34:18Z
dc.date.available.fl_str_mv 2020-10-14T01:34:18Z
dc.date.issued.fl_str_mv 2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SOUSA, Sergei Rodrigo Magalhães de et al. The use of the circulating cathodic antigen (Cca) urine cassette assay for the diagnosis and assessment of cure of schistosoma mansoni infections in an endemic. Revista da Sociedade Brasileira de Medicina Tropical, v. 53, e20190562, Sept. 2020.
dc.identifier.uri.fl_str_mv https://patua.iec.gov.br/handle/iec/4185
dc.identifier.issn.-.fl_str_mv 1678-9849
dc.identifier.doi.-.fl_str_mv 10.1590/0037-8682-0562-2019
identifier_str_mv SOUSA, Sergei Rodrigo Magalhães de et al. The use of the circulating cathodic antigen (Cca) urine cassette assay for the diagnosis and assessment of cure of schistosoma mansoni infections in an endemic. Revista da Sociedade Brasileira de Medicina Tropical, v. 53, e20190562, Sept. 2020.
1678-9849
10.1590/0037-8682-0562-2019
url https://patua.iec.gov.br/handle/iec/4185
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dc.publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical
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