The use of the circulating cathodic antigen (Cca) urine cassette assay for the diagnosis and assessment of cure of schistosoma mansoni infections in an endemic
Autor(a) principal: | |
---|---|
Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/4185 |
Resumo: | INTRODUCTION: Schistosomiasis is a poverty-related disease that affects people in 78 countries worldwide. This study aimed to evaluate the point-of-care circulating cathodic antigen (POC-CCA) test performance using sensitive parasitological methods as a reference standard (RS) in individuals before and after treatment. METHODS: The RS was established by combining the results of 16 Kato-Katz slides and the Helmintex® method. Positivity rates of the POC-CCA test and Kato-Katz and Helmintex® methods were calculated before treatment and 30 days afterward. Furthermore, the sensitivity, specificity, accuracy, and kappa coefficient before treatment were determined by comparing the methods. The cure rate was defined 30 days after treatment. RESULTS: Among the 217 participants, the RS detected a total of 63 (29.0%) positive individuals. The POC-CCA test identified 79 (36.4%) infections. The evaluation of POC-CCA test performance in relation to the RS revealed a sensitivity of 61.9%, specificity of 74.0%, accuracy of 70.5%, and kappa coefficient of 0.33. Out of the 53 remaining participants after treatment, a total of 45 (81.1%) showed egg negative results, and 8 (18.9%) were egg positive according to the RS. A total of 5 (9.4%) egg-positive and 37 (69.8%) egg-negative individuals were positive by the POC-CCA test. CONCLUSIONS: Our data show that the POC-CCA test has potential as an auxiliary tool for the diagnosis of Schistosoma mansoni infection, yielding better results than 16 Kato-Katz slides from three different stool samples. However, the immunochromatographic test lacks sufficient specificity and sensitivity for verifying the cure rate after treatment. |
id |
IEC-2_8898af994072844349f652f868851fad |
---|---|
oai_identifier_str |
oai:patua.iec.gov.br:iec/4185 |
network_acronym_str |
IEC-2 |
network_name_str |
Repositório Digital do Instituto Evandro Chagas (Patuá) |
repository_id_str |
|
spelling |
Sousa, Sergei Rodrigo Magalhães deNogueira, Joyce Favacho CardosoDias, Isabelle Helena LimaFonseca, Álvaro Luan SantanaFavero, VivianGeiger, Stefan MichaelEnk, Martin Johannes2020-10-14T01:34:18Z2020-10-14T01:34:18Z2020SOUSA, Sergei Rodrigo Magalhães de et al. The use of the circulating cathodic antigen (Cca) urine cassette assay for the diagnosis and assessment of cure of schistosoma mansoni infections in an endemic. Revista da Sociedade Brasileira de Medicina Tropical, v. 53, e20190562, Sept. 2020.1678-9849https://patua.iec.gov.br/handle/iec/418510.1590/0037-8682-0562-2019INTRODUCTION: Schistosomiasis is a poverty-related disease that affects people in 78 countries worldwide. This study aimed to evaluate the point-of-care circulating cathodic antigen (POC-CCA) test performance using sensitive parasitological methods as a reference standard (RS) in individuals before and after treatment. METHODS: The RS was established by combining the results of 16 Kato-Katz slides and the Helmintex® method. Positivity rates of the POC-CCA test and Kato-Katz and Helmintex® methods were calculated before treatment and 30 days afterward. Furthermore, the sensitivity, specificity, accuracy, and kappa coefficient before treatment were determined by comparing the methods. The cure rate was defined 30 days after treatment. RESULTS: Among the 217 participants, the RS detected a total of 63 (29.0%) positive individuals. The POC-CCA test identified 79 (36.4%) infections. The evaluation of POC-CCA test performance in relation to the RS revealed a sensitivity of 61.9%, specificity of 74.0%, accuracy of 70.5%, and kappa coefficient of 0.33. Out of the 53 remaining participants after treatment, a total of 45 (81.1%) showed egg negative results, and 8 (18.9%) were egg positive according to the RS. A total of 5 (9.4%) egg-positive and 37 (69.8%) egg-negative individuals were positive by the POC-CCA test. CONCLUSIONS: Our data show that the POC-CCA test has potential as an auxiliary tool for the diagnosis of Schistosoma mansoni infection, yielding better results than 16 Kato-Katz slides from three different stool samples. However, the immunochromatographic test lacks sufficient specificity and sensitivity for verifying the cure rate after treatment.Universidade do Estado do Pará. Programa de Pós-Graduação Strictu Sensu em Biologia Parasitária na Amazônia. Belém, PA, Brasil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Parasitoses Intestinais, Esquistossomose e Malacologia. Ananindeua, PA, Brasil.Universidade do Estado do Pará. Programa de Pós-Graduação Strictu Sensu em Biologia Parasitária na Amazônia. Belém, PA, Brasil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Parasitoses Intestinais, Esquistossomose e Malacologia. Ananindeua, PA, Brasil.Universidade do Estado do Pará. Programa de Pós-Graduação Strictu Sensu em Biologia Parasitária na Amazônia. Belém, PA, Brasil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Parasitoses Intestinais, Esquistossomose e Malacologia. Ananindeua, PA, Brasil.Universidade do Estado do Pará. Programa de Pós-Graduação Strictu Sensu em Biologia Parasitária na Amazônia. Belém, PA, Brasil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Parasitoses Intestinais, Esquistossomose e Malacologia. Ananindeua, PA, Brasil.Pontifícia Universidade Católica do Rio Grande do Sul. Programa de Pós-Graduação em Medicina e Ciências da Saúde. Laboratório de Parasitologia Biomédica. Porto Alegre, RS, Brasil.Universidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, Brasil.Universidade do Estado do Pará. Programa de Pós-Graduação Strictu Sensu em Biologia Parasitária na Amazônia. Belém, PA, Brasil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Parasitoses Intestinais, Esquistossomose e Malacologia. Ananindeua, PA, Brasil.engSociedade Brasileira de Medicina TropicalThe use of the circulating cathodic antigen (Cca) urine cassette assay for the diagnosis and assessment of cure of schistosoma mansoni infections in an endemicinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleEsquistossomose mansoni / parasitologiaEsquistossomose mansoni / diagnósticoTestes ImediatosSensibilidade e EspecificidadeContagem de Ovos de ParasitasKato-KatzHelmintex®info:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALThe use of the circulating cathodic antigen (Cca) urine cassette assay for the diagnosis and assessment of cure of schistosoma mansoni infections in an endemic.pdfThe use of the circulating cathodic antigen (Cca) urine cassette assay for the diagnosis and assessment of cure of schistosoma mansoni infections in an endemic.pdfapplication/pdf1065377https://patua.iec.gov.br/bitstreams/f31fb8f2-18df-4a18-9f1a-885a5c844ad3/download00aa9239fa337b02dc7ecbcbb37a605fMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82182https://patua.iec.gov.br/bitstreams/52fee4aa-3e0d-44c6-b65b-e7ab5c74ce99/download11832eea31b16df8613079d742d61793MD52TEXTThe use of the circulating cathodic antigen (Cca) urine cassette assay for the diagnosis and assessment of cure of schistosoma mansoni infections in an endemic.pdf.txtThe use of the circulating cathodic antigen (Cca) urine cassette assay for the diagnosis and assessment of cure of schistosoma mansoni infections in an endemic.pdf.txtExtracted texttext/plain35842https://patua.iec.gov.br/bitstreams/85d80297-a20c-42f7-99fa-9670d728816e/downloade98e4b1c8d618ccb8794cacc232b06e1MD55THUMBNAILThe use of the circulating cathodic antigen (Cca) urine cassette assay for the diagnosis and assessment of cure of schistosoma mansoni infections in an endemic.pdf.jpgThe use of the circulating cathodic antigen (Cca) urine cassette assay for the diagnosis and assessment of cure of schistosoma mansoni infections in an endemic.pdf.jpgGenerated Thumbnailimage/jpeg6614https://patua.iec.gov.br/bitstreams/5ea0b871-b287-45c1-a458-42df845d490f/downloadbb130d805da3f87f5698bd8cb7ee84b0MD56iec/41852023-03-07 16:53:01.76oai:patua.iec.gov.br:iec/4185https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2023-03-07T16:53:01Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)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 |
dc.title.pt_BR.fl_str_mv |
The use of the circulating cathodic antigen (Cca) urine cassette assay for the diagnosis and assessment of cure of schistosoma mansoni infections in an endemic |
title |
The use of the circulating cathodic antigen (Cca) urine cassette assay for the diagnosis and assessment of cure of schistosoma mansoni infections in an endemic |
spellingShingle |
The use of the circulating cathodic antigen (Cca) urine cassette assay for the diagnosis and assessment of cure of schistosoma mansoni infections in an endemic Sousa, Sergei Rodrigo Magalhães de Esquistossomose mansoni / parasitologia Esquistossomose mansoni / diagnóstico Testes Imediatos Sensibilidade e Especificidade Contagem de Ovos de Parasitas Kato-Katz Helmintex® |
title_short |
The use of the circulating cathodic antigen (Cca) urine cassette assay for the diagnosis and assessment of cure of schistosoma mansoni infections in an endemic |
title_full |
The use of the circulating cathodic antigen (Cca) urine cassette assay for the diagnosis and assessment of cure of schistosoma mansoni infections in an endemic |
title_fullStr |
The use of the circulating cathodic antigen (Cca) urine cassette assay for the diagnosis and assessment of cure of schistosoma mansoni infections in an endemic |
title_full_unstemmed |
The use of the circulating cathodic antigen (Cca) urine cassette assay for the diagnosis and assessment of cure of schistosoma mansoni infections in an endemic |
title_sort |
The use of the circulating cathodic antigen (Cca) urine cassette assay for the diagnosis and assessment of cure of schistosoma mansoni infections in an endemic |
author |
Sousa, Sergei Rodrigo Magalhães de |
author_facet |
Sousa, Sergei Rodrigo Magalhães de Nogueira, Joyce Favacho Cardoso Dias, Isabelle Helena Lima Fonseca, Álvaro Luan Santana Favero, Vivian Geiger, Stefan Michael Enk, Martin Johannes |
author_role |
author |
author2 |
Nogueira, Joyce Favacho Cardoso Dias, Isabelle Helena Lima Fonseca, Álvaro Luan Santana Favero, Vivian Geiger, Stefan Michael Enk, Martin Johannes |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Sousa, Sergei Rodrigo Magalhães de Nogueira, Joyce Favacho Cardoso Dias, Isabelle Helena Lima Fonseca, Álvaro Luan Santana Favero, Vivian Geiger, Stefan Michael Enk, Martin Johannes |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Esquistossomose mansoni / parasitologia Esquistossomose mansoni / diagnóstico Testes Imediatos Sensibilidade e Especificidade Contagem de Ovos de Parasitas Kato-Katz Helmintex® |
topic |
Esquistossomose mansoni / parasitologia Esquistossomose mansoni / diagnóstico Testes Imediatos Sensibilidade e Especificidade Contagem de Ovos de Parasitas Kato-Katz Helmintex® |
description |
INTRODUCTION: Schistosomiasis is a poverty-related disease that affects people in 78 countries worldwide. This study aimed to evaluate the point-of-care circulating cathodic antigen (POC-CCA) test performance using sensitive parasitological methods as a reference standard (RS) in individuals before and after treatment. METHODS: The RS was established by combining the results of 16 Kato-Katz slides and the Helmintex® method. Positivity rates of the POC-CCA test and Kato-Katz and Helmintex® methods were calculated before treatment and 30 days afterward. Furthermore, the sensitivity, specificity, accuracy, and kappa coefficient before treatment were determined by comparing the methods. The cure rate was defined 30 days after treatment. RESULTS: Among the 217 participants, the RS detected a total of 63 (29.0%) positive individuals. The POC-CCA test identified 79 (36.4%) infections. The evaluation of POC-CCA test performance in relation to the RS revealed a sensitivity of 61.9%, specificity of 74.0%, accuracy of 70.5%, and kappa coefficient of 0.33. Out of the 53 remaining participants after treatment, a total of 45 (81.1%) showed egg negative results, and 8 (18.9%) were egg positive according to the RS. A total of 5 (9.4%) egg-positive and 37 (69.8%) egg-negative individuals were positive by the POC-CCA test. CONCLUSIONS: Our data show that the POC-CCA test has potential as an auxiliary tool for the diagnosis of Schistosoma mansoni infection, yielding better results than 16 Kato-Katz slides from three different stool samples. However, the immunochromatographic test lacks sufficient specificity and sensitivity for verifying the cure rate after treatment. |
publishDate |
2020 |
dc.date.accessioned.fl_str_mv |
2020-10-14T01:34:18Z |
dc.date.available.fl_str_mv |
2020-10-14T01:34:18Z |
dc.date.issued.fl_str_mv |
2020 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SOUSA, Sergei Rodrigo Magalhães de et al. The use of the circulating cathodic antigen (Cca) urine cassette assay for the diagnosis and assessment of cure of schistosoma mansoni infections in an endemic. Revista da Sociedade Brasileira de Medicina Tropical, v. 53, e20190562, Sept. 2020. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/4185 |
dc.identifier.issn.-.fl_str_mv |
1678-9849 |
dc.identifier.doi.-.fl_str_mv |
10.1590/0037-8682-0562-2019 |
identifier_str_mv |
SOUSA, Sergei Rodrigo Magalhães de et al. The use of the circulating cathodic antigen (Cca) urine cassette assay for the diagnosis and assessment of cure of schistosoma mansoni infections in an endemic. Revista da Sociedade Brasileira de Medicina Tropical, v. 53, e20190562, Sept. 2020. 1678-9849 10.1590/0037-8682-0562-2019 |
url |
https://patua.iec.gov.br/handle/iec/4185 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Medicina Tropical |
publisher.none.fl_str_mv |
Sociedade Brasileira de Medicina Tropical |
dc.source.none.fl_str_mv |
reponame:Repositório Digital do Instituto Evandro Chagas (Patuá) instname:Instituto Evandro Chagas (IEC) instacron:IEC |
instname_str |
Instituto Evandro Chagas (IEC) |
instacron_str |
IEC |
institution |
IEC |
reponame_str |
Repositório Digital do Instituto Evandro Chagas (Patuá) |
collection |
Repositório Digital do Instituto Evandro Chagas (Patuá) |
bitstream.url.fl_str_mv |
https://patua.iec.gov.br/bitstreams/f31fb8f2-18df-4a18-9f1a-885a5c844ad3/download https://patua.iec.gov.br/bitstreams/52fee4aa-3e0d-44c6-b65b-e7ab5c74ce99/download https://patua.iec.gov.br/bitstreams/85d80297-a20c-42f7-99fa-9670d728816e/download https://patua.iec.gov.br/bitstreams/5ea0b871-b287-45c1-a458-42df845d490f/download |
bitstream.checksum.fl_str_mv |
00aa9239fa337b02dc7ecbcbb37a605f 11832eea31b16df8613079d742d61793 e98e4b1c8d618ccb8794cacc232b06e1 bb130d805da3f87f5698bd8cb7ee84b0 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC) |
repository.mail.fl_str_mv |
clariceneta@iec.gov.br || Biblioteca@iec.gov.br |
_version_ |
1809190042958036992 |