Evaluation of third-generation RIDASCREEN enzyme immunoassay for the detection of norovirus antigens in stool samples of hospitalized children in Belém, Pará, Brazil

• Autor(a) principal: Siqueira, Jones Anderson Monteiro
• Data de Publicação: 2011
• Outros Autores: Linhares, Alexandre da Costa, Oliveira, Darleise de Souza, Soares, Luana da Silva, Lucena, Maria Silvia Souza, Wanzeller, Ana Lúcia Monteiro, Mascarenhas, Joana D'Arc Pereira, Gabbay, Yvone Benchimol
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
• Texto Completo: https://patua.iec.gov.br/handle/iec/3297
• topic: Norovirus / isolamento & purificação; Vírion / ultraestrutura; Infecções por Caliciviridae / diagnóstico; Infecções por Caliciviridae / virologia; Gastroenterite / diagnóstico; Gastroenterite / virologia; Antígenos Virais / análise; RNA Viral / genética; Análise de Sequência de DNA; Fezes / virologia; Sensibilidade e Especificidade; Genótipo; Técnicas Imunoenzimáticas / métodos; Microscopia Eletrônica de Transmissão; Reação em Cadeia da Polimerase Via Transcriptase Reversa / métodos; Criança Hospitalizada

Noroviruses (NoVs) are major agents of gastroenteritis outbreaks and hospitalization worldwide. This study evaluated the sensitivity and specificity of the commercially available third-generation RIDASCREEN® Norovirus Enzyme Immunoassay (EIA) kit in comparison to the reverse transcription-polymerase chain reaction (RT-PCR) to detect NoVs in hospitalized children with gastroenteritis. An agreement of 88% (81/92) was observed when comparing EIA with RT-PCR. A sensitivity of 92% and a specificity of 83.3% were demonstrated. Eleven samples were positive by 1 method only (4 RT-PCR/7 EIA). Fourteen samples were sequenced and all classified as NoV genogroup GII-4. The 7 positive only by EIA were also evaluated by electron microscopy, and in 3 (42.9%) samples viral particles with a suggestive morphology of NoVs were visualized. These same samples were tested by seminested-RT-PCR with a positivity of 85.7%. The results obtained in this study demonstrated a significant improvement in the sensitivity and specificity of this updated assay