A single-dose live-attenuated vaccine prevents Zika virus pregnancy transmission and testis damage
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/2832 |
Resumo: | Zika virus infection during pregnancy can cause congenital abnormities or fetal demise. The persistence of Zika virus in the male reproductive system poses a risk of sexual transmission. Here we demonstrate that live-attenuated Zika virus vaccine candidates containing deletions in the 3' untranslated region of the Zika virus genome (ZIKV-3'UTR-LAV) prevent viral transmission during pregnancy and testis damage in mice, as well as infection of nonhuman primates. After a single-dose vaccination, pregnant mice challenged with Zika virus at embryonic day 6 and evaluated at embryonic day 13 show markedly diminished levels of viral RNA in maternal, placental, and fetal tissues. Vaccinated male mice challenged with Zika virus were protected against testis infection, injury, and oligospermia. A single immunization of rhesus macaques elicited a rapid and robust antibody response, conferring complete protection upon challenge. Furthermore, the ZIKV-3'UTR-LAV vaccine candidates have a desirable safety profile. These results suggest that further development of ZIKV-3'UTR-LAV is warranted for humans.Zika virus infection can result in congenital disorders and cause disease in adults, and there is currently no approved vaccine. Here Shan et al. show that a single dose of a live-attenuated Zika vaccine prevents infection, testis damage and transmission to the fetus during pregnancy in different animal models. |
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Shan, ChaoMuruato, Antonio E.Jagger, Brett W.Richner, JustinNunes, Bruno Tardelli DinizMedeiros, Daniele Barbosa de AlmeidaXie, XupingNunes, Jannyce G. C.Morabito, Kaitlyn M.Kong, Wing-PuiPierson, Theodore C.Barrett, Alan D.Weaver, Scott C.Rossi, Shannan L.Vasconcelos, Pedro Fernando da CostaGraham, Barney S.Diamond, Michael S.Shi, Pei-Yong2017-11-07T12:33:04Z2017-11-07T12:33:04Z2017SHAN, Chao et al. A single-dose live-attenuated vaccine prevents Zika virus pregnancy transmission and testis damage. Nature Communications, v. 22. n. 8, p. 676. Sept. 2017.2041-1723https://patua.iec.gov.br/handle/iec/283210.1038/s41467-017-00737-8.Zika virus infection during pregnancy can cause congenital abnormities or fetal demise. The persistence of Zika virus in the male reproductive system poses a risk of sexual transmission. Here we demonstrate that live-attenuated Zika virus vaccine candidates containing deletions in the 3' untranslated region of the Zika virus genome (ZIKV-3'UTR-LAV) prevent viral transmission during pregnancy and testis damage in mice, as well as infection of nonhuman primates. After a single-dose vaccination, pregnant mice challenged with Zika virus at embryonic day 6 and evaluated at embryonic day 13 show markedly diminished levels of viral RNA in maternal, placental, and fetal tissues. Vaccinated male mice challenged with Zika virus were protected against testis infection, injury, and oligospermia. A single immunization of rhesus macaques elicited a rapid and robust antibody response, conferring complete protection upon challenge. Furthermore, the ZIKV-3'UTR-LAV vaccine candidates have a desirable safety profile. These results suggest that further development of ZIKV-3'UTR-LAV is warranted for humans.Zika virus infection can result in congenital disorders and cause disease in adults, and there is currently no approved vaccine. Here Shan et al. show that a single dose of a live-attenuated Zika vaccine prevents infection, testis damage and transmission to the fetus during pregnancy in different animal models.Esta pesquisa também foi parcialmente apoiada pela concessão NIH AI120942 a S.C.W. e concede AI073755, AI104972 e AI106695 do NIH para M.S.D. P.F.C.V. foi suported por projetos de CAPES (Zika Fast-Track) e CNPq: bolsas 440405 / 2016-5 e 303999 / 2016-0 do Ministério da Ciência e Tecnologia de Brasil e pelo Ministério da Saúde.University of Texas Medical Branch. Department of Biochemistry & Molecular Biology. Galveston, TX, USA.University of Texas Medical Branch. Department of Microbiology & Immunology. Galveston, TX, USA.Washington University School of Medicine. Department of Medicine. St. Louis, MO, USA.Washington University School of Medicine. Department of Medicine. St. Louis, MO, USA.University of Texas Medical Branch. Department of Biochemistry & Molecular Biology. Galveston, TX, USA / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.University of Texas Medical Branch. Department of Biochemistry & Molecular Biology. Galveston, TX, USA / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.University of Texas Medical Branch. Department of Biochemistry & Molecular Biology. Galveston, TX, USA.University of Texas Medical Branch. Department of Biochemistry & Molecular Biology. Galveston, TX, USA / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.National Institutes of Health. National Institute of Allergy and Infectious Diseases. Vaccine Research Center. Bethesda, MD, USA.National Institutes of Health. National Institute of Allergy and Infectious Diseases. Vaccine Research Center. Bethesda, MD, USA.National Institutes of Health. National Institute of Allergy and Infectious Disease. Laboratory of Viral Diseases. Viral Pathogenesis Section. Bethesda, MD, USA.University of Texas Medical Branch. Institute for Human Infections & Immunity. Galveston, TX, USA / University of Texas Medical Branch. Department of Pathology. Galveston, TX, USA / University of Texas Medical Branch. Center for Biodefense & Emerging Infectious Diseases, , Galveston, TX, USA / University of Texas Medical Branch. Sealy Center for Vaccine Development. Galveston, TX, USA.University of Texas Medical Branch. Department of Microbiology & Immunology. Galveston, TX, USA / University of Texas Medical Branch. Sealy Center for Vaccine Development. Galveston, TX, USA / University of Texas Medical Branch. Institute for Translational Science. Galveston, TX, USA / University of Texas Medical Branch. Sealy Center for Structural Biology & Molecular Biophysics. Galveston, TX, USA.University of Texas Medical Branch. Institute for Human Infections & Immunity. Galveston, TX, USA / University of Texas Medical Branch. Department of Pathology. Galveston, TX, USA / University of Texas Medical Branch. Center for Biodefense & Emerging Infectious Diseases, , Galveston, TX, USA.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Pará State University. Department of Pathology. Belém, PA, Brazil.National Institutes of Health. National Institute of Allergy and Infectious Disease. Laboratory of Viral Diseases. Viral Pathogenesis Section. Bethesda, MD, USA.Washington University School of Medicine. Department of Medicine, , St. Louis, MO, USA / Washington University School of Medicine. Department of Molecular Microbiology. St. Louis, MO, USA / Washington University School of Medicine. Department of Pathology & Immunology. St. Louis, MO, USA / Washington University School of Medicine. The Andrew M. and Jane M. Bursky Center for Human Immunology and Immunotherapy Programs. St. Louis, MO, USAUniversity of Texas Medical Branch. Department of Biochemistry & Molecular Biology. Galveston, TX, USA / University of Texas Medical Branch. Sealy Center for Vaccine Development Galveston, TX, USA / University of Texas Medical Branch. Institute for Translational Science. Galveston, TX, USA / Pará State University. Department of Pathology. Belém, PA, Brazil / University of Texas Medical Branch. Department of Phamarcology & Toxicology. Galveston, TX, USA.engNature Publishing GroupA single-dose live-attenuated vaccine prevents Zika virus pregnancy transmission and testis damageinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleZika virusInfecção pelo Zika virusGravidezVacinas / administração & dosagemReprodução / fisiologiaGenitália MasculinaTransmissão de Doença InfecciosaFlavivirusinfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALA single-dose live-attenuated vaccine prevents Zika virus pregnancy transmission and testis damage.pdfA single-dose live-attenuated vaccine prevents Zika virus pregnancy transmission and testis damage.pdfapplication/pdf907981https://patua.iec.gov.br/bitstreams/98a16a0c-a748-468a-bd16-67635af55be6/download0b600e1b70c9cc9c1c20409a9944b047MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-871https://patua.iec.gov.br/bitstreams/6ccc500f-7d02-41d7-a01a-d3e949e9fab6/download52f1732ea66fbd1123abe39f5373b797MD52TEXTA single-dose live-attenuated vaccine prevents Zika virus pregnancy transmission and testis damage.pdf.txtA single-dose live-attenuated vaccine prevents Zika virus pregnancy transmission and testis damage.pdf.txtExtracted texttext/plain58083https://patua.iec.gov.br/bitstreams/d24eee65-7aca-4206-9170-92e7e81373ec/downloadc1d1b94b77b51832de71404c1f479bfaMD55THUMBNAILA single-dose live-attenuated vaccine prevents Zika virus pregnancy transmission and testis damage.pdf.jpgA single-dose live-attenuated vaccine prevents Zika virus pregnancy transmission and testis damage.pdf.jpgGenerated Thumbnailimage/jpeg5161https://patua.iec.gov.br/bitstreams/e3d48865-4747-47ab-9d45-40c8cf69e6ed/download4c6b4d807555f1580f4efa0ba7d8341dMD56iec/28322022-10-21 00:48:21.658oai:patua.iec.gov.br:iec/2832https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-21T00:48:21Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)falseVG9kb3Mgb3MgZG9jdW1lbnRvcyBkZXNzYSBjb2xlw6fDo28gc2VndWVtIGEgTGljZW7Dp2EgQ3JlYXRpdmUgY29tbW9ucy4= |
dc.title.pt_BR.fl_str_mv |
A single-dose live-attenuated vaccine prevents Zika virus pregnancy transmission and testis damage |
title |
A single-dose live-attenuated vaccine prevents Zika virus pregnancy transmission and testis damage |
spellingShingle |
A single-dose live-attenuated vaccine prevents Zika virus pregnancy transmission and testis damage Shan, Chao Zika virus Infecção pelo Zika virus Gravidez Vacinas / administração & dosagem Reprodução / fisiologia Genitália Masculina Transmissão de Doença Infecciosa Flavivirus |
title_short |
A single-dose live-attenuated vaccine prevents Zika virus pregnancy transmission and testis damage |
title_full |
A single-dose live-attenuated vaccine prevents Zika virus pregnancy transmission and testis damage |
title_fullStr |
A single-dose live-attenuated vaccine prevents Zika virus pregnancy transmission and testis damage |
title_full_unstemmed |
A single-dose live-attenuated vaccine prevents Zika virus pregnancy transmission and testis damage |
title_sort |
A single-dose live-attenuated vaccine prevents Zika virus pregnancy transmission and testis damage |
author |
Shan, Chao |
author_facet |
Shan, Chao Muruato, Antonio E. Jagger, Brett W. Richner, Justin Nunes, Bruno Tardelli Diniz Medeiros, Daniele Barbosa de Almeida Xie, Xuping Nunes, Jannyce G. C. Morabito, Kaitlyn M. Kong, Wing-Pui Pierson, Theodore C. Barrett, Alan D. Weaver, Scott C. Rossi, Shannan L. Vasconcelos, Pedro Fernando da Costa Graham, Barney S. Diamond, Michael S. Shi, Pei-Yong |
author_role |
author |
author2 |
Muruato, Antonio E. Jagger, Brett W. Richner, Justin Nunes, Bruno Tardelli Diniz Medeiros, Daniele Barbosa de Almeida Xie, Xuping Nunes, Jannyce G. C. Morabito, Kaitlyn M. Kong, Wing-Pui Pierson, Theodore C. Barrett, Alan D. Weaver, Scott C. Rossi, Shannan L. Vasconcelos, Pedro Fernando da Costa Graham, Barney S. Diamond, Michael S. Shi, Pei-Yong |
author2_role |
author author author author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Shan, Chao Muruato, Antonio E. Jagger, Brett W. Richner, Justin Nunes, Bruno Tardelli Diniz Medeiros, Daniele Barbosa de Almeida Xie, Xuping Nunes, Jannyce G. C. Morabito, Kaitlyn M. Kong, Wing-Pui Pierson, Theodore C. Barrett, Alan D. Weaver, Scott C. Rossi, Shannan L. Vasconcelos, Pedro Fernando da Costa Graham, Barney S. Diamond, Michael S. Shi, Pei-Yong |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Zika virus Infecção pelo Zika virus Gravidez Vacinas / administração & dosagem Reprodução / fisiologia Genitália Masculina Transmissão de Doença Infecciosa Flavivirus |
topic |
Zika virus Infecção pelo Zika virus Gravidez Vacinas / administração & dosagem Reprodução / fisiologia Genitália Masculina Transmissão de Doença Infecciosa Flavivirus |
description |
Zika virus infection during pregnancy can cause congenital abnormities or fetal demise. The persistence of Zika virus in the male reproductive system poses a risk of sexual transmission. Here we demonstrate that live-attenuated Zika virus vaccine candidates containing deletions in the 3' untranslated region of the Zika virus genome (ZIKV-3'UTR-LAV) prevent viral transmission during pregnancy and testis damage in mice, as well as infection of nonhuman primates. After a single-dose vaccination, pregnant mice challenged with Zika virus at embryonic day 6 and evaluated at embryonic day 13 show markedly diminished levels of viral RNA in maternal, placental, and fetal tissues. Vaccinated male mice challenged with Zika virus were protected against testis infection, injury, and oligospermia. A single immunization of rhesus macaques elicited a rapid and robust antibody response, conferring complete protection upon challenge. Furthermore, the ZIKV-3'UTR-LAV vaccine candidates have a desirable safety profile. These results suggest that further development of ZIKV-3'UTR-LAV is warranted for humans.Zika virus infection can result in congenital disorders and cause disease in adults, and there is currently no approved vaccine. Here Shan et al. show that a single dose of a live-attenuated Zika vaccine prevents infection, testis damage and transmission to the fetus during pregnancy in different animal models. |
publishDate |
2017 |
dc.date.accessioned.fl_str_mv |
2017-11-07T12:33:04Z |
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2017-11-07T12:33:04Z |
dc.date.issued.fl_str_mv |
2017 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SHAN, Chao et al. A single-dose live-attenuated vaccine prevents Zika virus pregnancy transmission and testis damage. Nature Communications, v. 22. n. 8, p. 676. Sept. 2017. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/2832 |
dc.identifier.issn.-.fl_str_mv |
2041-1723 |
dc.identifier.doi.-.fl_str_mv |
10.1038/s41467-017-00737-8. |
identifier_str_mv |
SHAN, Chao et al. A single-dose live-attenuated vaccine prevents Zika virus pregnancy transmission and testis damage. Nature Communications, v. 22. n. 8, p. 676. Sept. 2017. 2041-1723 10.1038/s41467-017-00737-8. |
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Nature Publishing Group |
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Nature Publishing Group |
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