Negeviruses isolated from mosquitoes in the Brazilian Amazon

• Autor(a) principal: Ribeiro, Ana Cláudia da Silva
• Data de Publicação: 2022
• Outros Autores: Martins, Lívia Caricio, Silva, Sandro Patroca da, Medeiros, Daniele Barbosa de Almeida, Miranda, Keissy Karoline Pinheiro, Nunes Neto, Joaquim Pinto, Monteiro, Hamilton Antônio de Oliveira, Nascimento, Bruna Lais Sena do, Rosa Junior, José Wilson, Cruz, Ana Cecília Ribeiro, Vasconcelos, Pedro Fernando da Costa, Carvalho, Valéria Lima, Rodrigues, Sueli Guerreiro
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
• Texto Completo: http://patua.iec.gov.br//handle/iec/4521
• topic: Arbovírus / classificação; Arbovírus / isolamento & purificação; Vírus de Insetos; Mosquitos Vetores; Filogenia; Vírus Cordoba; Vírus Negev; Vírus Feitosa

Background: There are several groups of viruses including Insect Specific Viruses (ISV) such as the taxon Negevirus, a group of viruses phylogenetically related to plant viruses. Negeviruses replicate in mosquito cells, but not in vertebrate cells. Methods: Pools of hematophagous arthropods were inoculated in Vero and C6/36 cells. The cells were observed to detect possible cytopathic effect. Then, indirect immunofluorescence, RT-PCR, and nucleotide sequencing were performed. Results: Seven samples which presented negative results for flaviviruses, alphaviruses and bunyaviruses, but showed cytopathic effect in C6/36 cells were sequenced. We identified the occurrence of a variety of ISVs, most of them belonging to the taxon Negevirus: The Brejeira, Negev, Cordoba and Wallerfield viruses, including a new virus for science, tentatively named Feitosa virus. Conclusions: We detected negeviruses in the Amazon region, including two viruses that were isolated for the first time in Brazil: Cordoba virus and the Negev virus and, a new virus for science: the Feitosa virus.