Phylogenetic relationship of dengue virus type 3 isolated in Brazil and Paraguay and global evolutionary divergence dynamics
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/377 |
Resumo: | Background: Dengue is the most important mosquito-borne viral disease worldwide. Dengue virus comprises four antigenically related viruses named dengue virus type 1 to 4 (DENV1-4). DENV-3 was re-introduced into the Americas in 1994 causing outbreaks in Nicaragua and Panama. DENV-3 was introduced in Brazil in 2000 and then spread to most of the Brazilian States, reaching the neighboring country, Paraguay in 2002. In this study, we have analyzed the phylogenetic relationship of DENV-3 isolated in Brazil and Paraguay with viruses isolated worldwide. We have also analyzed the evolutionary divergence dynamics of DENV-3 viruses. Results: The entire open reading frame (ORF) of thirteen DENV-3 isolated in Brazil (n = 9) and Paraguay (n = 4) were sequenced for phylogenetic analysis. DENV-3 grouped into three main genotypes (I, II and III). Several internal clades were found within each genotype that we called lineage and sub-lineage. Viruses included in this study belong to genotype III and grouped together with viruses isolated in the Americas within the lineage III. The Brazilian viruses were further segregated into two different sub-lineage, A and B, and the Paraguayan into the sublineage B. All three genotypes showed internal grouping. The nucleotide divergence was in average 6.7% for genotypes, 2.7% for lineages and 1.5% for sub-lineages. Phylogenetic trees constructed with any of the protein gene sequences showed the same segregation of the DENV-3 in three genotypes. Conclusion: Our results showed that two groups of DENV-3 genotypes III circulated in Brazil during 2002–2009, suggesting different events of introduction of the virus through different regions of the country. In Paraguay, only one group DENV-3 genotype III is circulating that is very closely related to the Brazilian viruses of sub-lineage B. Different degree of grouping can be observed for DENV-3 and each group showed a characteristic evolutionary divergence. Finally, we have observed that any protein gene sequence can be used to identify the virus genotype. |
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Alfonso, Helda LizAmarilla, Alberto AnastacioGonçalves, Paula FernandaBarros, Matheus TakatubaAlmeida, Flavia Tremeschin deSilva, Telma RSilva, Eliana Vieira Pinto daNunes, Márcio Roberto TeixeiraVasconcelos, Pedro Fernando da CostaVieira, Deusilene SBatista, Weber CheliBobadilla, Maria LizVazquez, CynthiaMoran, MirianFigueiredo, Luiz Tadeu MoraesAquino, Victor Hugo2016-01-26T11:22:40Z2016-01-26T11:22:40Z2012ALFONSO, Helda Liz et al. Phylogenetic relationship of dengue virus type 3 isolated in Brazil and Paraguay and global evolutionary divergence dynamics. Virology Journal, v. 9, p. 1-16, 2012. DOI: https://doi.org/10.1186/1743-422X-9-124. Disponível em: https://virologyj.biomedcentral.com/counter/pdf/10.1186/1743-422X-9-124.pdf.1743-422Xhttps://patua.iec.gov.br/handle/iec/37710.1186/1743-422X-9-124Background: Dengue is the most important mosquito-borne viral disease worldwide. Dengue virus comprises four antigenically related viruses named dengue virus type 1 to 4 (DENV1-4). DENV-3 was re-introduced into the Americas in 1994 causing outbreaks in Nicaragua and Panama. DENV-3 was introduced in Brazil in 2000 and then spread to most of the Brazilian States, reaching the neighboring country, Paraguay in 2002. In this study, we have analyzed the phylogenetic relationship of DENV-3 isolated in Brazil and Paraguay with viruses isolated worldwide. We have also analyzed the evolutionary divergence dynamics of DENV-3 viruses. Results: The entire open reading frame (ORF) of thirteen DENV-3 isolated in Brazil (n = 9) and Paraguay (n = 4) were sequenced for phylogenetic analysis. DENV-3 grouped into three main genotypes (I, II and III). Several internal clades were found within each genotype that we called lineage and sub-lineage. Viruses included in this study belong to genotype III and grouped together with viruses isolated in the Americas within the lineage III. The Brazilian viruses were further segregated into two different sub-lineage, A and B, and the Paraguayan into the sublineage B. All three genotypes showed internal grouping. The nucleotide divergence was in average 6.7% for genotypes, 2.7% for lineages and 1.5% for sub-lineages. Phylogenetic trees constructed with any of the protein gene sequences showed the same segregation of the DENV-3 in three genotypes. Conclusion: Our results showed that two groups of DENV-3 genotypes III circulated in Brazil during 2002–2009, suggesting different events of introduction of the virus through different regions of the country. In Paraguay, only one group DENV-3 genotype III is circulating that is very closely related to the Brazilian viruses of sub-lineage B. Different degree of grouping can be observed for DENV-3 and each group showed a characteristic evolutionary divergence. Finally, we have observed that any protein gene sequence can be used to identify the virus genotype.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas e Bromatológicas. Ribeirão Preto, SP, Brasil.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas e Bromatológicas. Ribeirão Preto, SP, Brasil.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas e Bromatológicas. Ribeirão Preto, SP, Brasil.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas e Bromatológicas. Ribeirão Preto, SP, Brasil.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas e Bromatológicas. Ribeirão Preto, SP, Brasil.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas e Bromatológicas. Ribeirão Preto, SP, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisas em Patologias Tropicais de Rondônia. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisas em Patologias Tropicais de Rondônia. Porto Velho, RO, Brasil.Ministerio de Salud y Bienestar Social. Departamento de Virología. Laboratorio Central de Salud Pública. Asunción, Paraguay.Ministerio de Salud y Bienestar Social. Departamento de Virología. Laboratorio Central de Salud Pública. Asunción, Paraguay.Ministerio de Salud y Bienestar Social. Departamento de Virología. Laboratorio Central de Salud Pública. Asunción, Paraguay.Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Centro de Pesquisa em Virologia. Ribeirão Preto, SP, Brasil.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas e Bromatológicas. 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dc.title.pt_BR.fl_str_mv |
Phylogenetic relationship of dengue virus type 3 isolated in Brazil and Paraguay and global evolutionary divergence dynamics |
title |
Phylogenetic relationship of dengue virus type 3 isolated in Brazil and Paraguay and global evolutionary divergence dynamics |
spellingShingle |
Phylogenetic relationship of dengue virus type 3 isolated in Brazil and Paraguay and global evolutionary divergence dynamics Alfonso, Helda Liz Vírus da Dengue / isolamento & purificação Dengue / transmissão Região Amazônica (BR) |
title_short |
Phylogenetic relationship of dengue virus type 3 isolated in Brazil and Paraguay and global evolutionary divergence dynamics |
title_full |
Phylogenetic relationship of dengue virus type 3 isolated in Brazil and Paraguay and global evolutionary divergence dynamics |
title_fullStr |
Phylogenetic relationship of dengue virus type 3 isolated in Brazil and Paraguay and global evolutionary divergence dynamics |
title_full_unstemmed |
Phylogenetic relationship of dengue virus type 3 isolated in Brazil and Paraguay and global evolutionary divergence dynamics |
title_sort |
Phylogenetic relationship of dengue virus type 3 isolated in Brazil and Paraguay and global evolutionary divergence dynamics |
author |
Alfonso, Helda Liz |
author_facet |
Alfonso, Helda Liz Amarilla, Alberto Anastacio Gonçalves, Paula Fernanda Barros, Matheus Takatuba Almeida, Flavia Tremeschin de Silva, Telma R Silva, Eliana Vieira Pinto da Nunes, Márcio Roberto Teixeira Vasconcelos, Pedro Fernando da Costa Vieira, Deusilene S Batista, Weber Cheli Bobadilla, Maria Liz Vazquez, Cynthia Moran, Mirian Figueiredo, Luiz Tadeu Moraes Aquino, Victor Hugo |
author_role |
author |
author2 |
Amarilla, Alberto Anastacio Gonçalves, Paula Fernanda Barros, Matheus Takatuba Almeida, Flavia Tremeschin de Silva, Telma R Silva, Eliana Vieira Pinto da Nunes, Márcio Roberto Teixeira Vasconcelos, Pedro Fernando da Costa Vieira, Deusilene S Batista, Weber Cheli Bobadilla, Maria Liz Vazquez, Cynthia Moran, Mirian Figueiredo, Luiz Tadeu Moraes Aquino, Victor Hugo |
author2_role |
author author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Alfonso, Helda Liz Amarilla, Alberto Anastacio Gonçalves, Paula Fernanda Barros, Matheus Takatuba Almeida, Flavia Tremeschin de Silva, Telma R Silva, Eliana Vieira Pinto da Nunes, Márcio Roberto Teixeira Vasconcelos, Pedro Fernando da Costa Vieira, Deusilene S Batista, Weber Cheli Bobadilla, Maria Liz Vazquez, Cynthia Moran, Mirian Figueiredo, Luiz Tadeu Moraes Aquino, Victor Hugo |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Vírus da Dengue / isolamento & purificação Dengue / transmissão Região Amazônica (BR) |
topic |
Vírus da Dengue / isolamento & purificação Dengue / transmissão Região Amazônica (BR) |
description |
Background: Dengue is the most important mosquito-borne viral disease worldwide. Dengue virus comprises four antigenically related viruses named dengue virus type 1 to 4 (DENV1-4). DENV-3 was re-introduced into the Americas in 1994 causing outbreaks in Nicaragua and Panama. DENV-3 was introduced in Brazil in 2000 and then spread to most of the Brazilian States, reaching the neighboring country, Paraguay in 2002. In this study, we have analyzed the phylogenetic relationship of DENV-3 isolated in Brazil and Paraguay with viruses isolated worldwide. We have also analyzed the evolutionary divergence dynamics of DENV-3 viruses. Results: The entire open reading frame (ORF) of thirteen DENV-3 isolated in Brazil (n = 9) and Paraguay (n = 4) were sequenced for phylogenetic analysis. DENV-3 grouped into three main genotypes (I, II and III). Several internal clades were found within each genotype that we called lineage and sub-lineage. Viruses included in this study belong to genotype III and grouped together with viruses isolated in the Americas within the lineage III. The Brazilian viruses were further segregated into two different sub-lineage, A and B, and the Paraguayan into the sublineage B. All three genotypes showed internal grouping. The nucleotide divergence was in average 6.7% for genotypes, 2.7% for lineages and 1.5% for sub-lineages. Phylogenetic trees constructed with any of the protein gene sequences showed the same segregation of the DENV-3 in three genotypes. Conclusion: Our results showed that two groups of DENV-3 genotypes III circulated in Brazil during 2002–2009, suggesting different events of introduction of the virus through different regions of the country. In Paraguay, only one group DENV-3 genotype III is circulating that is very closely related to the Brazilian viruses of sub-lineage B. Different degree of grouping can be observed for DENV-3 and each group showed a characteristic evolutionary divergence. Finally, we have observed that any protein gene sequence can be used to identify the virus genotype. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012 |
dc.date.accessioned.fl_str_mv |
2016-01-26T11:22:40Z |
dc.date.available.fl_str_mv |
2016-01-26T11:22:40Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
ALFONSO, Helda Liz et al. Phylogenetic relationship of dengue virus type 3 isolated in Brazil and Paraguay and global evolutionary divergence dynamics. Virology Journal, v. 9, p. 1-16, 2012. DOI: https://doi.org/10.1186/1743-422X-9-124. Disponível em: https://virologyj.biomedcentral.com/counter/pdf/10.1186/1743-422X-9-124.pdf. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/377 |
dc.identifier.issn.-.fl_str_mv |
1743-422X |
dc.identifier.doi.pt_BR.fl_str_mv |
10.1186/1743-422X-9-124 |
identifier_str_mv |
ALFONSO, Helda Liz et al. Phylogenetic relationship of dengue virus type 3 isolated in Brazil and Paraguay and global evolutionary divergence dynamics. Virology Journal, v. 9, p. 1-16, 2012. DOI: https://doi.org/10.1186/1743-422X-9-124. Disponível em: https://virologyj.biomedcentral.com/counter/pdf/10.1186/1743-422X-9-124.pdf. 1743-422X 10.1186/1743-422X-9-124 |
url |
https://patua.iec.gov.br/handle/iec/377 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
BioMed Central |
publisher.none.fl_str_mv |
BioMed Central |
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reponame:Repositório Digital do Instituto Evandro Chagas (Patuá) instname:Instituto Evandro Chagas (IEC) instacron:IEC |
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Repositório Digital do Instituto Evandro Chagas (Patuá) |
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