Emergence of new immunopathogenic factors in human yellow fever: polarization of the M1/M2 macrophage response in the renal parenchyma
Autor(a) principal: | |
---|---|
Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/4657 |
Resumo: | Macrophages in the kidney play a pathogenic role in inflammation and fibrosis. Our study aimed to understand the polarisation of the M1 and M2 phenotypic profiles of macrophages in injured kidney tissue retrieved from fatal cases of yellow fever virus (YFV). A total of 11 renal tissue biopsies obtained from patients who died of yellow fever (YF) were analysed. To detect antibodies that promote the classical and alternative pathways of macrophage activation, immunohistochemical analysis was performed to detect CD163, CD68, inducible nitric oxide synthase (iNOS), arginase 1, interleukin (IL)-4, IL-10, interferon (IFN)-γ, IFN-β, tumour necrosis factor (TNF)-α, IL-13, and transforming growth factor (TGF)-β. There was a difference in the marker expression between fatal cases of YFV and control samples, with increased expression in the cortical region of the renal parenchyma. The immunoexpression of CD68 and CD163 receptors suggests the presence of activated macrophages migrating to infectious foci. The rise in IL-10, IL-4, and IL-13 indicated their potential role in the inactivation of the inflammatory macrophage response and phenotypic modulation of M2 macrophages. The altered expression of IFN-γ and IFN-β demonstrates the importance of the innate immune response in combating microorganisms. Our findings indicate that the polarisation of M1 and M2 macrophages plays a vital role in the renal immune response to YFV. |
id |
IEC-2_a09f50b5ebf678c26ab2e742a7e96f8c |
---|---|
oai_identifier_str |
oai:patua.iec.gov.br:iec/4657 |
network_acronym_str |
IEC-2 |
network_name_str |
Repositório Digital do Instituto Evandro Chagas (Patuá) |
repository_id_str |
|
spelling |
Melo, Juliana MarinhoFalcão, Luiz Fabio MagnoPonte, Lucas Coutinho Tuma daSilva, Camilla CostaMartins, Lívia CaricioChiang, Jannifer OliveiraMartins Filho, Arnaldo JorgeFranco, Edna Cristina SantosDuarte, Maria Irna SeixasSousa, Jorge Rodrigues deVasconcelos, Pedro Fernando da CostaQuaresma, Juarez Antônio Simões2022-09-12T13:19:28Z2022-09-12T13:19:28Z2022MELO, Juliana Marinho et al. Emergence of new immunopathogenic factors in human yellow fever: polarization of the M1/M2 macrophage response in the renal parenchyma. Viruses, v. 14, n. 8, p. 1-11, Aug. 2022. DOI: https://doi.org/10.3390/v14081725. Disponível em: https://www.mdpi.com/1999-4915/14/8/1725/htm1999-4915https://patua.iec.gov.br/handle/iec/465710.3390/v14081725Macrophages in the kidney play a pathogenic role in inflammation and fibrosis. Our study aimed to understand the polarisation of the M1 and M2 phenotypic profiles of macrophages in injured kidney tissue retrieved from fatal cases of yellow fever virus (YFV). A total of 11 renal tissue biopsies obtained from patients who died of yellow fever (YF) were analysed. To detect antibodies that promote the classical and alternative pathways of macrophage activation, immunohistochemical analysis was performed to detect CD163, CD68, inducible nitric oxide synthase (iNOS), arginase 1, interleukin (IL)-4, IL-10, interferon (IFN)-γ, IFN-β, tumour necrosis factor (TNF)-α, IL-13, and transforming growth factor (TGF)-β. There was a difference in the marker expression between fatal cases of YFV and control samples, with increased expression in the cortical region of the renal parenchyma. The immunoexpression of CD68 and CD163 receptors suggests the presence of activated macrophages migrating to infectious foci. The rise in IL-10, IL-4, and IL-13 indicated their potential role in the inactivation of the inflammatory macrophage response and phenotypic modulation of M2 macrophages. The altered expression of IFN-γ and IFN-β demonstrates the importance of the innate immune response in combating microorganisms. Our findings indicate that the polarisation of M1 and M2 macrophages plays a vital role in the renal immune response to YFV.This research was funded by grant number 457664/2013-4 and 303999/2016-0.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Universidade do Estado do Pará. Departamento de Patologia. Belém, PA, Brazil / Universidade de São Paulo. Faculdade de Medicina. São Paulo, SP, Brazil.Universidade do Estado do Pará. Departamento de Patologia. Belém, PA, Brazil.Universidade do Estado do Pará. Departamento de Patologia. Belém, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Universidade de São Paulo. Faculdade de Medicina. São Paulo, SP, Brazil.Universidade do Estado do Pará. Departamento de Patologia. Belém, PA, Brazil.Universidade do Estado do Pará. Departamento de Patologia. Belém, PA, Brazil.Universidade do Estado do Pará. Departamento de Patologia. Belém, PA, Brazil / Universidade de São Paulo. Faculdade de Medicina. São Paulo, SP, Brazil / Universidade Federal do Pará. Núcleo de Medicina Tropical. Belém, PA, Brazil.engMDPIEmergence of new immunopathogenic factors in human yellow fever: polarization of the M1/M2 macrophage response in the renal parenchymainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFebre AmarelaRim / anatomia & histologiaRim / lesõesAntígeno de Macrófago 1 / análiseSistema Imunitárioinfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALEmergence of new immunopathogenic factors in human yellow fever: polarization of the M1/M2 macrophage response in the renal parenchyma.pdfEmergence of new immunopathogenic factors in human yellow fever: polarization of the M1/M2 macrophage response in the renal parenchyma.pdfapplication/pdf1690717https://patua.iec.gov.br/bitstreams/0408c52f-0c93-46de-819e-b4dc9ce774d6/download9f643a461dd031ab61a758945f0dc627MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82182https://patua.iec.gov.br/bitstreams/08679e69-8564-4997-a0ba-4b19fada0e82/download11832eea31b16df8613079d742d61793MD52TEXTEmergence of new immunopathogenic factors in human yellow fever: polarization of the M1/M2 macrophage response in the renal parenchyma.pdf.txtEmergence of new immunopathogenic factors in human yellow fever: polarization of the M1/M2 macrophage response in the renal parenchyma.pdf.txtExtracted texttext/plain46623https://patua.iec.gov.br/bitstreams/7d52cf3c-84db-404f-a218-c0d0710f7c5e/download9486f7e4c55785030c8c6c01696e4cbaMD55THUMBNAILEmergence of new immunopathogenic factors in human yellow fever: polarization of the M1/M2 macrophage response in the renal parenchyma.pdf.jpgEmergence of new immunopathogenic factors in human yellow fever: polarization of the M1/M2 macrophage response in the renal parenchyma.pdf.jpgGenerated Thumbnailimage/jpeg5890https://patua.iec.gov.br/bitstreams/d9752db1-a29a-4bc7-b8f1-59e7ffabb0a5/download717f31918e715a0f0aad48db7c49f7d7MD56iec/46572023-03-15 13:35:31.682oai:patua.iec.gov.br:iec/4657https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2023-03-15T13:35:31Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)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 |
dc.title.pt_BR.fl_str_mv |
Emergence of new immunopathogenic factors in human yellow fever: polarization of the M1/M2 macrophage response in the renal parenchyma |
title |
Emergence of new immunopathogenic factors in human yellow fever: polarization of the M1/M2 macrophage response in the renal parenchyma |
spellingShingle |
Emergence of new immunopathogenic factors in human yellow fever: polarization of the M1/M2 macrophage response in the renal parenchyma Melo, Juliana Marinho Febre Amarela Rim / anatomia & histologia Rim / lesões Antígeno de Macrófago 1 / análise Sistema Imunitário |
title_short |
Emergence of new immunopathogenic factors in human yellow fever: polarization of the M1/M2 macrophage response in the renal parenchyma |
title_full |
Emergence of new immunopathogenic factors in human yellow fever: polarization of the M1/M2 macrophage response in the renal parenchyma |
title_fullStr |
Emergence of new immunopathogenic factors in human yellow fever: polarization of the M1/M2 macrophage response in the renal parenchyma |
title_full_unstemmed |
Emergence of new immunopathogenic factors in human yellow fever: polarization of the M1/M2 macrophage response in the renal parenchyma |
title_sort |
Emergence of new immunopathogenic factors in human yellow fever: polarization of the M1/M2 macrophage response in the renal parenchyma |
author |
Melo, Juliana Marinho |
author_facet |
Melo, Juliana Marinho Falcão, Luiz Fabio Magno Ponte, Lucas Coutinho Tuma da Silva, Camilla Costa Martins, Lívia Caricio Chiang, Jannifer Oliveira Martins Filho, Arnaldo Jorge Franco, Edna Cristina Santos Duarte, Maria Irna Seixas Sousa, Jorge Rodrigues de Vasconcelos, Pedro Fernando da Costa Quaresma, Juarez Antônio Simões |
author_role |
author |
author2 |
Falcão, Luiz Fabio Magno Ponte, Lucas Coutinho Tuma da Silva, Camilla Costa Martins, Lívia Caricio Chiang, Jannifer Oliveira Martins Filho, Arnaldo Jorge Franco, Edna Cristina Santos Duarte, Maria Irna Seixas Sousa, Jorge Rodrigues de Vasconcelos, Pedro Fernando da Costa Quaresma, Juarez Antônio Simões |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Melo, Juliana Marinho Falcão, Luiz Fabio Magno Ponte, Lucas Coutinho Tuma da Silva, Camilla Costa Martins, Lívia Caricio Chiang, Jannifer Oliveira Martins Filho, Arnaldo Jorge Franco, Edna Cristina Santos Duarte, Maria Irna Seixas Sousa, Jorge Rodrigues de Vasconcelos, Pedro Fernando da Costa Quaresma, Juarez Antônio Simões |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Febre Amarela Rim / anatomia & histologia Rim / lesões Antígeno de Macrófago 1 / análise Sistema Imunitário |
topic |
Febre Amarela Rim / anatomia & histologia Rim / lesões Antígeno de Macrófago 1 / análise Sistema Imunitário |
description |
Macrophages in the kidney play a pathogenic role in inflammation and fibrosis. Our study aimed to understand the polarisation of the M1 and M2 phenotypic profiles of macrophages in injured kidney tissue retrieved from fatal cases of yellow fever virus (YFV). A total of 11 renal tissue biopsies obtained from patients who died of yellow fever (YF) were analysed. To detect antibodies that promote the classical and alternative pathways of macrophage activation, immunohistochemical analysis was performed to detect CD163, CD68, inducible nitric oxide synthase (iNOS), arginase 1, interleukin (IL)-4, IL-10, interferon (IFN)-γ, IFN-β, tumour necrosis factor (TNF)-α, IL-13, and transforming growth factor (TGF)-β. There was a difference in the marker expression between fatal cases of YFV and control samples, with increased expression in the cortical region of the renal parenchyma. The immunoexpression of CD68 and CD163 receptors suggests the presence of activated macrophages migrating to infectious foci. The rise in IL-10, IL-4, and IL-13 indicated their potential role in the inactivation of the inflammatory macrophage response and phenotypic modulation of M2 macrophages. The altered expression of IFN-γ and IFN-β demonstrates the importance of the innate immune response in combating microorganisms. Our findings indicate that the polarisation of M1 and M2 macrophages plays a vital role in the renal immune response to YFV. |
publishDate |
2022 |
dc.date.accessioned.fl_str_mv |
2022-09-12T13:19:28Z |
dc.date.available.fl_str_mv |
2022-09-12T13:19:28Z |
dc.date.issued.fl_str_mv |
2022 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MELO, Juliana Marinho et al. Emergence of new immunopathogenic factors in human yellow fever: polarization of the M1/M2 macrophage response in the renal parenchyma. Viruses, v. 14, n. 8, p. 1-11, Aug. 2022. DOI: https://doi.org/10.3390/v14081725. Disponível em: https://www.mdpi.com/1999-4915/14/8/1725/htm |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/4657 |
dc.identifier.issn.-.fl_str_mv |
1999-4915 |
dc.identifier.doi.pt_BR.fl_str_mv |
10.3390/v14081725 |
identifier_str_mv |
MELO, Juliana Marinho et al. Emergence of new immunopathogenic factors in human yellow fever: polarization of the M1/M2 macrophage response in the renal parenchyma. Viruses, v. 14, n. 8, p. 1-11, Aug. 2022. DOI: https://doi.org/10.3390/v14081725. Disponível em: https://www.mdpi.com/1999-4915/14/8/1725/htm 1999-4915 10.3390/v14081725 |
url |
https://patua.iec.gov.br/handle/iec/4657 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Digital do Instituto Evandro Chagas (Patuá) instname:Instituto Evandro Chagas (IEC) instacron:IEC |
instname_str |
Instituto Evandro Chagas (IEC) |
instacron_str |
IEC |
institution |
IEC |
reponame_str |
Repositório Digital do Instituto Evandro Chagas (Patuá) |
collection |
Repositório Digital do Instituto Evandro Chagas (Patuá) |
bitstream.url.fl_str_mv |
https://patua.iec.gov.br/bitstreams/0408c52f-0c93-46de-819e-b4dc9ce774d6/download https://patua.iec.gov.br/bitstreams/08679e69-8564-4997-a0ba-4b19fada0e82/download https://patua.iec.gov.br/bitstreams/7d52cf3c-84db-404f-a218-c0d0710f7c5e/download https://patua.iec.gov.br/bitstreams/d9752db1-a29a-4bc7-b8f1-59e7ffabb0a5/download |
bitstream.checksum.fl_str_mv |
9f643a461dd031ab61a758945f0dc627 11832eea31b16df8613079d742d61793 9486f7e4c55785030c8c6c01696e4cba 717f31918e715a0f0aad48db7c49f7d7 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC) |
repository.mail.fl_str_mv |
clariceneta@iec.gov.br || Biblioteca@iec.gov.br |
_version_ |
1809190040853544960 |