Inhibition of melanization by kojic acid promotes cell wall disruption of the human pathogenic fungus Fonsecaea sp.

Detalhes bibliográficos
Autor(a) principal: Pereira, Jorge Augusto Leão
Data de Publicação: 2022
Outros Autores: Moraes, Lienne Silveira de, Sena, Chubert Bernardo Castro de, Nascimento, José Luiz Martins do, Rodrigues, Ana Paula D, Silva, Silvia Helena Marques da, Silva, Edilene Oliveira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
Texto Completo: http://patua.iec.gov.br/handle/iec/4664
Resumo: Chromoblastomycosis (CBM) is a chronic human subcutaneous mycosis caused by various aetiologic agents. CBM does not have an established treatment but may be managed using antifungal agents, surgical removal of the lesions, or cryotherapy. Kojic acid (KA), a known tyrosinase inhibitor with a variety of biological actions, including fungistatic action against the fungus Cryptococcus neoformans, mediated by inhibiting melanin production, seems to be an alternative to improve the treatment of CBM. The aim of the present study was to analyze the action of KA against the pathogenic fungus Fonsecaea sp., an aetiological agent of CBM. The fungal culture was incubated with KA, and the amount of melanin was assessed, followed by cytochemical detection. Subsequently, the samples were analyzed by light microscopy, transmission and scanning electron microscopy. Culture analysis revealed that 100 g/mL KA significantly decreased the melanization of the fungus and the exocytosis of melanin into the culture supernatant. Additionally, KA induced less growth of biofilm formation and intense disruption of the cell wall, and decreased the number of melanin-containing vesicles in the culture supernatant. Finally, KA inhibited fungal filamentation in culture and the subsequent phagocytosis process. Thus, KA may be a promising substance to help in the treatment of CBM
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spelling Pereira, Jorge Augusto LeãoMoraes, Lienne Silveira deSena, Chubert Bernardo Castro deNascimento, José Luiz Martins doRodrigues, Ana Paula DSilva, Silvia Helena Marques daSilva, Edilene Oliveira2022-09-13T13:41:33Z2022-09-13T13:41:33Z2022PEREIRA, Jorge Augusto Leão et al. Inhibition of melanization by kojic acid promotes cell wall disruption of the human pathogenic fungus Fonsecaea sp. Pathogens, v. 11, n. 8, p. 1-14, Aug. 2022. DOI: https://doi.org/10.3390/pathogens11080925. Disponível em: http://patua.iec.gov.br/handle/iec/46642076-0817http://patua.iec.gov.br/handle/iec/466410.3390/pathogens11080925Chromoblastomycosis (CBM) is a chronic human subcutaneous mycosis caused by various aetiologic agents. CBM does not have an established treatment but may be managed using antifungal agents, surgical removal of the lesions, or cryotherapy. Kojic acid (KA), a known tyrosinase inhibitor with a variety of biological actions, including fungistatic action against the fungus Cryptococcus neoformans, mediated by inhibiting melanin production, seems to be an alternative to improve the treatment of CBM. The aim of the present study was to analyze the action of KA against the pathogenic fungus Fonsecaea sp., an aetiological agent of CBM. The fungal culture was incubated with KA, and the amount of melanin was assessed, followed by cytochemical detection. Subsequently, the samples were analyzed by light microscopy, transmission and scanning electron microscopy. Culture analysis revealed that 100 g/mL KA significantly decreased the melanization of the fungus and the exocytosis of melanin into the culture supernatant. Additionally, KA induced less growth of biofilm formation and intense disruption of the cell wall, and decreased the number of melanin-containing vesicles in the culture supernatant. Finally, KA inhibited fungal filamentation in culture and the subsequent phagocytosis process. Thus, KA may be a promising substance to help in the treatment of CBMInstituto Nacional de Biologia Estrutural e Bioimagem-INBEB (CNPq—grant number 465395/2014); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior— Brasil (CAPES)—Finance Code 001; Pró-reitoria de Pesquisa da Universidade Federal do Pará (UFPA/PROPESP -PAPQ-Edital 02/2022).Federal University of Pará. Institute of Biological Sciences. Laboratory of Structural Biology. Belém, PA, Brazil.Federal University of Pará. Institute of Biological Sciences. Laboratory of Structural Biology. Belém, PA, Brazil / National Institute of Science and Technology in Structural Biology and Bioimaging. Rio de Janeiro, RJ, Brazil / Federal University of Amapá. Health and Biological Sciences Department. Pharmaceutical Sciences Post Graduation Program. Macapá, AP, Brazil.Federal University of Pará. Institute of Biological Sciences. Laboratory of Structural Biology. Belém, PA, Brazil / National Institute of Science and Technology in Neuroimmunomodulation. Rio de Janeiro, RJ, Brazil.Federal University of Amapá. Health and Biological Sciences Department. Pharmaceutical Sciences Post Graduation Program. Macapá, AP, Brazil / National Institute of Science and Technology in Neuroimmunomodulation. Rio de Janeiro, RJ, Brazil / Federal University of Pará. Institute of Biological Sciences. Laboratory of Molecular and Cellular Neurochemistry. Belém, PA, Brazil.National Institute of Science and Technology in Structural Biology and Bioimaging. Rio de Janeiro, RJ, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Microscopia Eletrônica. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Micoses Superficiais e Sistêmicas. Ananindeua, PA, Brasil.Federal University of Pará. Institute of Biological Sciences. Laboratory of Structural Biology. Belém, PA, Brazil / National Institute of Science and Technology in Structural Biology and Bioimaging. Rio de Janeiro, RJ, Brazil.engMDPIInhibition of melanization by kojic acid promotes cell wall disruption of the human pathogenic fungus Fonsecaea sp.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCromoblastomicose / tratamento farmacológicoFonsecaea / patogenicidadeAntifúngicosÁcido Kójico / químicainfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALInhibition of melanization by kojic acid promotes cell wall disruption of the human pathogenic fungus Fonsecaea sp..pdfInhibition of melanization by kojic acid promotes cell wall disruption of the human pathogenic fungus Fonsecaea sp..pdfapplication/pdf3183001https://patua.iec.gov.br/bitstreams/2c1e2506-579a-44ea-a9c0-3889e55941fe/downloadb88fbc0e11b247380965be16e441cbe3MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82182https://patua.iec.gov.br/bitstreams/2377097a-e700-47d9-89b2-c3c3853c8ae3/download11832eea31b16df8613079d742d61793MD52TEXTInhibition of melanization by kojic acid promotes cell wall disruption of the human pathogenic fungus Fonsecaea sp..pdf.txtInhibition of melanization by kojic acid promotes cell wall disruption of the human pathogenic fungus Fonsecaea sp..pdf.txtExtracted texttext/plain64044https://patua.iec.gov.br/bitstreams/0028a278-7bc2-4fbe-bb47-c592297df5fe/downloade773c0e8e6d577b1bff9de94b2886ce2MD55THUMBNAILInhibition of melanization by kojic acid promotes cell wall disruption of the human pathogenic fungus Fonsecaea sp..pdf.jpgInhibition of melanization by kojic acid promotes cell wall disruption of the human pathogenic fungus Fonsecaea sp..pdf.jpgGenerated Thumbnailimage/jpeg5676https://patua.iec.gov.br/bitstreams/3cb87eb3-d5bb-41c9-a06d-084e85c34b63/download65906a649b874060937a3ae7a58059c1MD56iec/46642022-10-20 22:14:22.241oai:patua.iec.gov.br:iec/4664https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-20T22:14:22Repositório Digital do Instituto Evandro Chagas (Patuá) - 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dc.title.pt_BR.fl_str_mv Inhibition of melanization by kojic acid promotes cell wall disruption of the human pathogenic fungus Fonsecaea sp.
title Inhibition of melanization by kojic acid promotes cell wall disruption of the human pathogenic fungus Fonsecaea sp.
spellingShingle Inhibition of melanization by kojic acid promotes cell wall disruption of the human pathogenic fungus Fonsecaea sp.
Pereira, Jorge Augusto Leão
Cromoblastomicose / tratamento farmacológico
Fonsecaea / patogenicidade
Antifúngicos
Ácido Kójico / química
title_short Inhibition of melanization by kojic acid promotes cell wall disruption of the human pathogenic fungus Fonsecaea sp.
title_full Inhibition of melanization by kojic acid promotes cell wall disruption of the human pathogenic fungus Fonsecaea sp.
title_fullStr Inhibition of melanization by kojic acid promotes cell wall disruption of the human pathogenic fungus Fonsecaea sp.
title_full_unstemmed Inhibition of melanization by kojic acid promotes cell wall disruption of the human pathogenic fungus Fonsecaea sp.
title_sort Inhibition of melanization by kojic acid promotes cell wall disruption of the human pathogenic fungus Fonsecaea sp.
author Pereira, Jorge Augusto Leão
author_facet Pereira, Jorge Augusto Leão
Moraes, Lienne Silveira de
Sena, Chubert Bernardo Castro de
Nascimento, José Luiz Martins do
Rodrigues, Ana Paula D
Silva, Silvia Helena Marques da
Silva, Edilene Oliveira
author_role author
author2 Moraes, Lienne Silveira de
Sena, Chubert Bernardo Castro de
Nascimento, José Luiz Martins do
Rodrigues, Ana Paula D
Silva, Silvia Helena Marques da
Silva, Edilene Oliveira
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pereira, Jorge Augusto Leão
Moraes, Lienne Silveira de
Sena, Chubert Bernardo Castro de
Nascimento, José Luiz Martins do
Rodrigues, Ana Paula D
Silva, Silvia Helena Marques da
Silva, Edilene Oliveira
dc.subject.decsPrimary.pt_BR.fl_str_mv Cromoblastomicose / tratamento farmacológico
Fonsecaea / patogenicidade
Antifúngicos
Ácido Kójico / química
topic Cromoblastomicose / tratamento farmacológico
Fonsecaea / patogenicidade
Antifúngicos
Ácido Kójico / química
description Chromoblastomycosis (CBM) is a chronic human subcutaneous mycosis caused by various aetiologic agents. CBM does not have an established treatment but may be managed using antifungal agents, surgical removal of the lesions, or cryotherapy. Kojic acid (KA), a known tyrosinase inhibitor with a variety of biological actions, including fungistatic action against the fungus Cryptococcus neoformans, mediated by inhibiting melanin production, seems to be an alternative to improve the treatment of CBM. The aim of the present study was to analyze the action of KA against the pathogenic fungus Fonsecaea sp., an aetiological agent of CBM. The fungal culture was incubated with KA, and the amount of melanin was assessed, followed by cytochemical detection. Subsequently, the samples were analyzed by light microscopy, transmission and scanning electron microscopy. Culture analysis revealed that 100 g/mL KA significantly decreased the melanization of the fungus and the exocytosis of melanin into the culture supernatant. Additionally, KA induced less growth of biofilm formation and intense disruption of the cell wall, and decreased the number of melanin-containing vesicles in the culture supernatant. Finally, KA inhibited fungal filamentation in culture and the subsequent phagocytosis process. Thus, KA may be a promising substance to help in the treatment of CBM
publishDate 2022
dc.date.accessioned.fl_str_mv 2022-09-13T13:41:33Z
dc.date.available.fl_str_mv 2022-09-13T13:41:33Z
dc.date.issued.fl_str_mv 2022
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PEREIRA, Jorge Augusto Leão et al. Inhibition of melanization by kojic acid promotes cell wall disruption of the human pathogenic fungus Fonsecaea sp. Pathogens, v. 11, n. 8, p. 1-14, Aug. 2022. DOI: https://doi.org/10.3390/pathogens11080925. Disponível em: http://patua.iec.gov.br/handle/iec/4664
dc.identifier.uri.fl_str_mv http://patua.iec.gov.br/handle/iec/4664
dc.identifier.issn.-.fl_str_mv 2076-0817
dc.identifier.doi.pt_BR.fl_str_mv 10.3390/pathogens11080925
identifier_str_mv PEREIRA, Jorge Augusto Leão et al. Inhibition of melanization by kojic acid promotes cell wall disruption of the human pathogenic fungus Fonsecaea sp. Pathogens, v. 11, n. 8, p. 1-14, Aug. 2022. DOI: https://doi.org/10.3390/pathogens11080925. Disponível em: http://patua.iec.gov.br/handle/iec/4664
2076-0817
10.3390/pathogens11080925
url http://patua.iec.gov.br/handle/iec/4664
dc.language.iso.fl_str_mv eng
language eng
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collection Repositório Digital do Instituto Evandro Chagas (Patuá)
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