Antiplasmodial activity of the andiroba (Carapa guianensis Aubl., Meliaceae) oil and its limonoid-rich fraction

Detalhes bibliográficos
Autor(a) principal: Miranda Júnior, Raimundo Nonato Cardoso
Data de Publicação: 2012
Outros Autores: Dolabela, Maria Fâni, Silva, Milton Nascimento da, Póvoa, Marinete Marins, Maia, José Guilherme S
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
Texto Completo: https://patua.iec.gov.br/handle/iec/865
Resumo: Ethnopharmacological relevance: From seeds of Carapa guianensis the Amazon native people extracts the andiroba oil, which is traditionally used as febrifuge, anti-malarial, insecticidal and repellant. The nonsaponifiable fraction separated from the oil is rich in limonoids, which assigns its pharmacological effects. Materials and methods: The andiroba oil and its limonoid-rich fraction were submitted to in vitro antiplasmodial bioassay using W2 and Dd2 strains of Plasmodium falciparum. The acute toxicity of andiroba oil was evaluated. The limonoid-rich fraction was subjected to fractionation and identified its major constituents. Results: Andiroba oil and its limonoid-rich fraction inhibited the growth of W2 clone in 100%, between 24 and 72 h, at concentrations of 8.2 mg/mL and 3.1 mg/mL, respectively. Under the same conditions, the parasitaemia of Dd2 clone provoked by the andiroba oil showed inhibition of 31% (IC50 482 mg/mL) with a time-dependent relationship of 24 h and inhibition of 88% (IC50 8.4 mg/mL) after 72 h, while for the limonoid-rich fraction the inhibition of Dd2 clone was 56% (IC50 2.8 mg/mL) at 24 h and 82% (IC50 0.4 mg/mL) after 72 h. Andiroba oil in acute toxicity test with a fixed dose (LD50 42000 mg/kg) was not toxic The limonoids identified in the oil were gedunin, 6a-acetoxygedunin, 7-deacetoxy-7-oxogedunin, 7-deacetylgedunin, 1,2-dihydro-3b-hydroxy-7-deacetoxy-7-oxogedunin and andirobin. Gedunin and derivatives has been reputed as anti-malarials. Conclusion: The results support the traditional use of andiroba oil as antiplasmodial, which additionally proved not to be toxic in bioassays conducted with mice
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spelling Miranda Júnior, Raimundo Nonato CardosoDolabela, Maria FâniSilva, Milton Nascimento daPóvoa, Marinete MarinsMaia, José Guilherme S2016-01-26T11:37:00Z2016-01-26T11:37:00Z2012MIRANDA JUNIOR, Raimundo Nonato Cardoso et al. Antiplasmodial activity of the andiroba (Carapa guianensis Aubl., Meliaceae) oil and its limonoid-rich fraction. Journal of Ethnopharmacology, v. 142, n. 3, p. 679-683, 2012.https://patua.iec.gov.br/handle/iec/86510.1016/j.jep.2012.05.037Ethnopharmacological relevance: From seeds of Carapa guianensis the Amazon native people extracts the andiroba oil, which is traditionally used as febrifuge, anti-malarial, insecticidal and repellant. The nonsaponifiable fraction separated from the oil is rich in limonoids, which assigns its pharmacological effects. Materials and methods: The andiroba oil and its limonoid-rich fraction were submitted to in vitro antiplasmodial bioassay using W2 and Dd2 strains of Plasmodium falciparum. The acute toxicity of andiroba oil was evaluated. The limonoid-rich fraction was subjected to fractionation and identified its major constituents. Results: Andiroba oil and its limonoid-rich fraction inhibited the growth of W2 clone in 100%, between 24 and 72 h, at concentrations of 8.2 mg/mL and 3.1 mg/mL, respectively. Under the same conditions, the parasitaemia of Dd2 clone provoked by the andiroba oil showed inhibition of 31% (IC50 482 mg/mL) with a time-dependent relationship of 24 h and inhibition of 88% (IC50 8.4 mg/mL) after 72 h, while for the limonoid-rich fraction the inhibition of Dd2 clone was 56% (IC50 2.8 mg/mL) at 24 h and 82% (IC50 0.4 mg/mL) after 72 h. Andiroba oil in acute toxicity test with a fixed dose (LD50 42000 mg/kg) was not toxic The limonoids identified in the oil were gedunin, 6a-acetoxygedunin, 7-deacetoxy-7-oxogedunin, 7-deacetylgedunin, 1,2-dihydro-3b-hydroxy-7-deacetoxy-7-oxogedunin and andirobin. Gedunin and derivatives has been reputed as anti-malarials. Conclusion: The results support the traditional use of andiroba oil as antiplasmodial, which additionally proved not to be toxic in bioassays conducted with miceUniversidade Federal do Pará. Programa de Pós-Graduação em Ciências Farmacêuticas. Belém, PA, Brazil.Universidade Federal do Pará. Programa de Pós-Graduação em Ciências Farmacêuticas. Belém, PA, Brazil.Universidade Federal do Pará. Programa de Pós-Graduação em Química. Belém, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Universidade Federal do Pará. Programa de Pós-Graduação em Ciências Farmacêuticas. Belém, PA, Brazil / Universidade Federal do Pará. Programa de Pós-Graduação em Química. 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dc.title.pt_BR.fl_str_mv Antiplasmodial activity of the andiroba (Carapa guianensis Aubl., Meliaceae) oil and its limonoid-rich fraction
title Antiplasmodial activity of the andiroba (Carapa guianensis Aubl., Meliaceae) oil and its limonoid-rich fraction
spellingShingle Antiplasmodial activity of the andiroba (Carapa guianensis Aubl., Meliaceae) oil and its limonoid-rich fraction
Miranda Júnior, Raimundo Nonato Cardoso
Meliaceae
Limoninas
Extratos Vegetais / farmacologia
Andiroba
Repelentes de Insetos
Etnofarmacologia / métodos
Antimaláricos
Plasmodium falciparum / parasitologia
Plasmodium falciparum / patogenicidade
title_short Antiplasmodial activity of the andiroba (Carapa guianensis Aubl., Meliaceae) oil and its limonoid-rich fraction
title_full Antiplasmodial activity of the andiroba (Carapa guianensis Aubl., Meliaceae) oil and its limonoid-rich fraction
title_fullStr Antiplasmodial activity of the andiroba (Carapa guianensis Aubl., Meliaceae) oil and its limonoid-rich fraction
title_full_unstemmed Antiplasmodial activity of the andiroba (Carapa guianensis Aubl., Meliaceae) oil and its limonoid-rich fraction
title_sort Antiplasmodial activity of the andiroba (Carapa guianensis Aubl., Meliaceae) oil and its limonoid-rich fraction
author Miranda Júnior, Raimundo Nonato Cardoso
author_facet Miranda Júnior, Raimundo Nonato Cardoso
Dolabela, Maria Fâni
Silva, Milton Nascimento da
Póvoa, Marinete Marins
Maia, José Guilherme S
author_role author
author2 Dolabela, Maria Fâni
Silva, Milton Nascimento da
Póvoa, Marinete Marins
Maia, José Guilherme S
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Miranda Júnior, Raimundo Nonato Cardoso
Dolabela, Maria Fâni
Silva, Milton Nascimento da
Póvoa, Marinete Marins
Maia, José Guilherme S
dc.subject.decsPrimary.pt_BR.fl_str_mv Meliaceae
Limoninas
Extratos Vegetais / farmacologia
Andiroba
Repelentes de Insetos
Etnofarmacologia / métodos
Antimaláricos
Plasmodium falciparum / parasitologia
Plasmodium falciparum / patogenicidade
topic Meliaceae
Limoninas
Extratos Vegetais / farmacologia
Andiroba
Repelentes de Insetos
Etnofarmacologia / métodos
Antimaláricos
Plasmodium falciparum / parasitologia
Plasmodium falciparum / patogenicidade
description Ethnopharmacological relevance: From seeds of Carapa guianensis the Amazon native people extracts the andiroba oil, which is traditionally used as febrifuge, anti-malarial, insecticidal and repellant. The nonsaponifiable fraction separated from the oil is rich in limonoids, which assigns its pharmacological effects. Materials and methods: The andiroba oil and its limonoid-rich fraction were submitted to in vitro antiplasmodial bioassay using W2 and Dd2 strains of Plasmodium falciparum. The acute toxicity of andiroba oil was evaluated. The limonoid-rich fraction was subjected to fractionation and identified its major constituents. Results: Andiroba oil and its limonoid-rich fraction inhibited the growth of W2 clone in 100%, between 24 and 72 h, at concentrations of 8.2 mg/mL and 3.1 mg/mL, respectively. Under the same conditions, the parasitaemia of Dd2 clone provoked by the andiroba oil showed inhibition of 31% (IC50 482 mg/mL) with a time-dependent relationship of 24 h and inhibition of 88% (IC50 8.4 mg/mL) after 72 h, while for the limonoid-rich fraction the inhibition of Dd2 clone was 56% (IC50 2.8 mg/mL) at 24 h and 82% (IC50 0.4 mg/mL) after 72 h. Andiroba oil in acute toxicity test with a fixed dose (LD50 42000 mg/kg) was not toxic The limonoids identified in the oil were gedunin, 6a-acetoxygedunin, 7-deacetoxy-7-oxogedunin, 7-deacetylgedunin, 1,2-dihydro-3b-hydroxy-7-deacetoxy-7-oxogedunin and andirobin. Gedunin and derivatives has been reputed as anti-malarials. Conclusion: The results support the traditional use of andiroba oil as antiplasmodial, which additionally proved not to be toxic in bioassays conducted with mice
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2016-01-26T11:37:00Z
dc.date.available.fl_str_mv 2016-01-26T11:37:00Z
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dc.identifier.citation.fl_str_mv MIRANDA JUNIOR, Raimundo Nonato Cardoso et al. Antiplasmodial activity of the andiroba (Carapa guianensis Aubl., Meliaceae) oil and its limonoid-rich fraction. Journal of Ethnopharmacology, v. 142, n. 3, p. 679-683, 2012.
dc.identifier.uri.fl_str_mv https://patua.iec.gov.br/handle/iec/865
dc.identifier.doi.-.fl_str_mv 10.1016/j.jep.2012.05.037
identifier_str_mv MIRANDA JUNIOR, Raimundo Nonato Cardoso et al. Antiplasmodial activity of the andiroba (Carapa guianensis Aubl., Meliaceae) oil and its limonoid-rich fraction. Journal of Ethnopharmacology, v. 142, n. 3, p. 679-683, 2012.
10.1016/j.jep.2012.05.037
url https://patua.iec.gov.br/handle/iec/865
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