Pseudomonas aeruginosa type III secretion system virulotypes and their association with clinical features of cystic fibrosis patients
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/4193 |
Resumo: | Purpose: Pseudomonas aeruginosa appears as the main pathogen in cystic fibrosis (CF) involved in recurrent pneumonia and pulmonary exacerbations. The type III secretion system (T3SS) is one of its main determinants of virulence and is associated with poor clinical progression and increased mortality. This study determined the relationship of clinical features of patients with CF and P. aeruginosa T3SS virulotypes. Materials and Methods: From January 2018 to March 2019, P. aeruginosa were isolated from sputum and/or oropharyngeal swabs. T3SS markers (exoS, exoU, exoT and exoY) were detected by PCR. Clinical severity according to Shwachman-Kulckycki score and spirometry data were associated with T3SS virulotypes. Results: A total of 49 patients had positive cultures for P. aeruginosa. T3SS virulence-related markers were detected as follows: exoS 97.9% (n=48), exoU 63.2% (n=31), exoT 95.9% (n=47) and exoY 97.9% (n=48). The prevalence of exoS+/exoU+ virulotype was higher than previously reported in CF settings, being detected in 61.2% of the evaluated isolates, present in 70% of intermittent infections and with a significantly higher frequency in cases of exacerba-tions. The presence of exoU in chronic infection was not associated with poor clinical results. In chronic infections, the exoS+/exoU− virulotype prevailed (77.8%) and was associated to worse clinical results according to the Shwachman-Kulckycki score and spirometric. Conclusion: Our findings revealed a high prevalence of the atypical exoS+ /exoU+ virulo-type among P. aeruginosa isolates from patients with CF, which was associated with intermittent infection and early clinical alterations, while the exoS+ /exoU− virulotype was associated with chronic infection and worse clinical results. Finally, the presented data highlight the relevance of T3SS virulence markers in the clinical progression and disease severity in CF patients. |
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Sarges, Edilene do Socorro Nascimento FalcãoRodrigues, Yan CorrêaFurlaneto, Ismari PeriniMelo, Marcos Vinicios Hino deBrabo, Giulia Leão da CunhaLopes, Kátia Cilene MachadoQuaresma, Ana Judith Pires GarciaLima, Luana Nepomuceno Gondim CostaLima, Karla Valéria Batista2020-11-03T14:59:14Z2020-11-03T14:59:14Z2020SARGES, Edilene do Socorro Nascimento Falcão et al. Pseudomonas aeruginosa type III secretion system virulotypes and their association with clinical features of cystic fibrosis patients. Infection and Drug Resistance, v. 13, p. 3771-3781, Oct. 2020.1178-6973https://patua.iec.gov.br/handle/iec/419310.2147/IDR.S273759Purpose: Pseudomonas aeruginosa appears as the main pathogen in cystic fibrosis (CF) involved in recurrent pneumonia and pulmonary exacerbations. The type III secretion system (T3SS) is one of its main determinants of virulence and is associated with poor clinical progression and increased mortality. This study determined the relationship of clinical features of patients with CF and P. aeruginosa T3SS virulotypes. Materials and Methods: From January 2018 to March 2019, P. aeruginosa were isolated from sputum and/or oropharyngeal swabs. T3SS markers (exoS, exoU, exoT and exoY) were detected by PCR. Clinical severity according to Shwachman-Kulckycki score and spirometry data were associated with T3SS virulotypes. Results: A total of 49 patients had positive cultures for P. aeruginosa. T3SS virulence-related markers were detected as follows: exoS 97.9% (n=48), exoU 63.2% (n=31), exoT 95.9% (n=47) and exoY 97.9% (n=48). The prevalence of exoS+/exoU+ virulotype was higher than previously reported in CF settings, being detected in 61.2% of the evaluated isolates, present in 70% of intermittent infections and with a significantly higher frequency in cases of exacerba-tions. The presence of exoU in chronic infection was not associated with poor clinical results. In chronic infections, the exoS+/exoU− virulotype prevailed (77.8%) and was associated to worse clinical results according to the Shwachman-Kulckycki score and spirometric. Conclusion: Our findings revealed a high prevalence of the atypical exoS+ /exoU+ virulo-type among P. aeruginosa isolates from patients with CF, which was associated with intermittent infection and early clinical alterations, while the exoS+ /exoU− virulotype was associated with chronic infection and worse clinical results. Finally, the presented data highlight the relevance of T3SS virulence markers in the clinical progression and disease severity in CF patients.This research was supported by funding from Fundação de Amparo à Pesquisa do Pará/Universidade do Estado do Pará (FAPESPA/UEPA) [Cooperation grant Nº004/2019], Programa Institucional de Bolsas de Iniciação Científica/ Fundação Amazônia Paraense de Amparo à Pesquisa/ Universidade do Estado do Pará (FAPESPA/UEPA) [Cooperation grant Nº001/2017/PPGBPA], Programa Institucional de Bolsas de Iniciação Científica/Fundação Amazônia Paraense de Amparo à Pesquisa/Universidade do Estado do Pará (FAPESPA/UEPA) [Cooperation grant Nº002/2019/Graduação], Programa Institucional de Bolsas de Iniciação Científica/Instituto Evandro Chagas – Conselho Nacional de Desenvolvimento Científico e Tecnológico (PIBIC/IEC/CNPq) and Instituto Evandro Chagas/Ministério da Saúde/Secretaria de Vigilância em Saúde (IEC/MS/SVS).Universidade do Estado do Pará. Programa de Pós-graduação em Biologia Parasitária na Amazônia. Belém, PA, Brazil / Universidade Federal do Pará. Hospital Universitário João de Barros Barreto. Belém, PA, Brazil.Universidade do Estado do Pará. Programa de Pós-graduação em Biologia Parasitária na Amazônia. Belém, PA, Brazil.Centro Universitário do Pará. Mestrado Profissional em Ensino em Saúde – Educação Médica. Belém, PA, Brazil.Universidade do Estado do Pará. Programa de Pós-graduação em Biologia Parasitária na Amazônia. Belém, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Universidade Federal do Pará. Hospital Universitário João de Barros Barreto. Belém, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Universidade do Estado do Pará. Programa de Pós-graduação em Biologia Parasitária na Amazônia. Belém, PA, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Universidade do Estado do Pará. Programa de Pós-graduação em Biologia Parasitária na Amazônia. Belém, PA, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.engDove Medical PressPseudomonas aeruginosa type III secretion system virulotypes and their association with clinical features of cystic fibrosis patientsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFibrose CísticaPseudomonas aeruginosa / patogenicidadeInfecções por Pseudomonas / microbiologiaVirulênciainfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALPseudomonas aeruginosa type III secretion system virulotypes and their association with clinical features of cystic fibrosis patients.pdfPseudomonas aeruginosa type III secretion system virulotypes and their association with clinical features of cystic fibrosis patients.pdfapplication/pdf362439https://patua.iec.gov.br/bitstreams/515bbe61-1562-4c09-a0d6-3600cbe23a9b/download20b5fb47c883ed4e3e5731360a06d8b1MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82182https://patua.iec.gov.br/bitstreams/3e8e003e-078f-49b5-a175-09eadbc646c4/download11832eea31b16df8613079d742d61793MD52TEXTPseudomonas aeruginosa type III secretion system virulotypes and their association with clinical features of cystic fibrosis patients.pdf.txtPseudomonas aeruginosa type III secretion system virulotypes and their association with clinical features of cystic fibrosis patients.pdf.txtExtracted texttext/plain55779https://patua.iec.gov.br/bitstreams/64ff0cb7-68e8-4e58-bb23-c7f92e7ef39e/downloade1c8764d99913fc5785ce7d9311a2e27MD55THUMBNAILPseudomonas aeruginosa type III secretion system virulotypes and their association with clinical features of cystic fibrosis patients.pdf.jpgPseudomonas aeruginosa type III secretion system virulotypes and their association with clinical features of cystic fibrosis patients.pdf.jpgGenerated Thumbnailimage/jpeg6318https://patua.iec.gov.br/bitstreams/d61d4c39-20fa-4961-bc30-761f290dd88c/downloadb85001235906f90728f8734c694769bfMD56iec/41932022-10-21 00:17:16.828oai:patua.iec.gov.br:iec/4193https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-21T00:17:16Repositório Digital do Instituto Evandro Chagas (Patuá) - 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dc.title.pt_BR.fl_str_mv |
Pseudomonas aeruginosa type III secretion system virulotypes and their association with clinical features of cystic fibrosis patients |
title |
Pseudomonas aeruginosa type III secretion system virulotypes and their association with clinical features of cystic fibrosis patients |
spellingShingle |
Pseudomonas aeruginosa type III secretion system virulotypes and their association with clinical features of cystic fibrosis patients Sarges, Edilene do Socorro Nascimento Falcão Fibrose Cística Pseudomonas aeruginosa / patogenicidade Infecções por Pseudomonas / microbiologia Virulência |
title_short |
Pseudomonas aeruginosa type III secretion system virulotypes and their association with clinical features of cystic fibrosis patients |
title_full |
Pseudomonas aeruginosa type III secretion system virulotypes and their association with clinical features of cystic fibrosis patients |
title_fullStr |
Pseudomonas aeruginosa type III secretion system virulotypes and their association with clinical features of cystic fibrosis patients |
title_full_unstemmed |
Pseudomonas aeruginosa type III secretion system virulotypes and their association with clinical features of cystic fibrosis patients |
title_sort |
Pseudomonas aeruginosa type III secretion system virulotypes and their association with clinical features of cystic fibrosis patients |
author |
Sarges, Edilene do Socorro Nascimento Falcão |
author_facet |
Sarges, Edilene do Socorro Nascimento Falcão Rodrigues, Yan Corrêa Furlaneto, Ismari Perini Melo, Marcos Vinicios Hino de Brabo, Giulia Leão da Cunha Lopes, Kátia Cilene Machado Quaresma, Ana Judith Pires Garcia Lima, Luana Nepomuceno Gondim Costa Lima, Karla Valéria Batista |
author_role |
author |
author2 |
Rodrigues, Yan Corrêa Furlaneto, Ismari Perini Melo, Marcos Vinicios Hino de Brabo, Giulia Leão da Cunha Lopes, Kátia Cilene Machado Quaresma, Ana Judith Pires Garcia Lima, Luana Nepomuceno Gondim Costa Lima, Karla Valéria Batista |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Sarges, Edilene do Socorro Nascimento Falcão Rodrigues, Yan Corrêa Furlaneto, Ismari Perini Melo, Marcos Vinicios Hino de Brabo, Giulia Leão da Cunha Lopes, Kátia Cilene Machado Quaresma, Ana Judith Pires Garcia Lima, Luana Nepomuceno Gondim Costa Lima, Karla Valéria Batista |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Fibrose Cística Pseudomonas aeruginosa / patogenicidade Infecções por Pseudomonas / microbiologia Virulência |
topic |
Fibrose Cística Pseudomonas aeruginosa / patogenicidade Infecções por Pseudomonas / microbiologia Virulência |
description |
Purpose: Pseudomonas aeruginosa appears as the main pathogen in cystic fibrosis (CF) involved in recurrent pneumonia and pulmonary exacerbations. The type III secretion system (T3SS) is one of its main determinants of virulence and is associated with poor clinical progression and increased mortality. This study determined the relationship of clinical features of patients with CF and P. aeruginosa T3SS virulotypes. Materials and Methods: From January 2018 to March 2019, P. aeruginosa were isolated from sputum and/or oropharyngeal swabs. T3SS markers (exoS, exoU, exoT and exoY) were detected by PCR. Clinical severity according to Shwachman-Kulckycki score and spirometry data were associated with T3SS virulotypes. Results: A total of 49 patients had positive cultures for P. aeruginosa. T3SS virulence-related markers were detected as follows: exoS 97.9% (n=48), exoU 63.2% (n=31), exoT 95.9% (n=47) and exoY 97.9% (n=48). The prevalence of exoS+/exoU+ virulotype was higher than previously reported in CF settings, being detected in 61.2% of the evaluated isolates, present in 70% of intermittent infections and with a significantly higher frequency in cases of exacerba-tions. The presence of exoU in chronic infection was not associated with poor clinical results. In chronic infections, the exoS+/exoU− virulotype prevailed (77.8%) and was associated to worse clinical results according to the Shwachman-Kulckycki score and spirometric. Conclusion: Our findings revealed a high prevalence of the atypical exoS+ /exoU+ virulo-type among P. aeruginosa isolates from patients with CF, which was associated with intermittent infection and early clinical alterations, while the exoS+ /exoU− virulotype was associated with chronic infection and worse clinical results. Finally, the presented data highlight the relevance of T3SS virulence markers in the clinical progression and disease severity in CF patients. |
publishDate |
2020 |
dc.date.accessioned.fl_str_mv |
2020-11-03T14:59:14Z |
dc.date.available.fl_str_mv |
2020-11-03T14:59:14Z |
dc.date.issued.fl_str_mv |
2020 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SARGES, Edilene do Socorro Nascimento Falcão et al. Pseudomonas aeruginosa type III secretion system virulotypes and their association with clinical features of cystic fibrosis patients. Infection and Drug Resistance, v. 13, p. 3771-3781, Oct. 2020. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/4193 |
dc.identifier.issn.-.fl_str_mv |
1178-6973 |
dc.identifier.doi.-.fl_str_mv |
10.2147/IDR.S273759 |
identifier_str_mv |
SARGES, Edilene do Socorro Nascimento Falcão et al. Pseudomonas aeruginosa type III secretion system virulotypes and their association with clinical features of cystic fibrosis patients. Infection and Drug Resistance, v. 13, p. 3771-3781, Oct. 2020. 1178-6973 10.2147/IDR.S273759 |
url |
https://patua.iec.gov.br/handle/iec/4193 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
Dove Medical Press |
publisher.none.fl_str_mv |
Dove Medical Press |
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