Pseudomonas aeruginosa type III secretion system virulotypes and their association with clinical features of cystic fibrosis patients

Detalhes bibliográficos
Autor(a) principal: Sarges, Edilene do Socorro Nascimento Falcão
Data de Publicação: 2020
Outros Autores: Rodrigues, Yan Corrêa, Furlaneto, Ismari Perini, Melo, Marcos Vinicios Hino de, Brabo, Giulia Leão da Cunha, Lopes, Kátia Cilene Machado, Quaresma, Ana Judith Pires Garcia, Lima, Luana Nepomuceno Gondim Costa, Lima, Karla Valéria Batista
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
Texto Completo: https://patua.iec.gov.br/handle/iec/4193
Resumo: Purpose: Pseudomonas aeruginosa appears as the main pathogen in cystic fibrosis (CF) involved in recurrent pneumonia and pulmonary exacerbations. The type III secretion system (T3SS) is one of its main determinants of virulence and is associated with poor clinical progression and increased mortality. This study determined the relationship of clinical features of patients with CF and P. aeruginosa T3SS virulotypes. Materials and Methods: From January 2018 to March 2019, P. aeruginosa were isolated from sputum and/or oropharyngeal swabs. T3SS markers (exoS, exoU, exoT and exoY) were detected by PCR. Clinical severity according to Shwachman-Kulckycki score and spirometry data were associated with T3SS virulotypes. Results: A total of 49 patients had positive cultures for P. aeruginosa. T3SS virulence-related markers were detected as follows: exoS 97.9% (n=48), exoU 63.2% (n=31), exoT 95.9% (n=47) and exoY 97.9% (n=48). The prevalence of exoS+/exoU+ virulotype was higher than previously reported in CF settings, being detected in 61.2% of the evaluated isolates, present in 70% of intermittent infections and with a significantly higher frequency in cases of exacerba-tions. The presence of exoU in chronic infection was not associated with poor clinical results. In chronic infections, the exoS+/exoU− virulotype prevailed (77.8%) and was associated to worse clinical results according to the Shwachman-Kulckycki score and spirometric. Conclusion: Our findings revealed a high prevalence of the atypical exoS+ /exoU+ virulo-type among P. aeruginosa isolates from patients with CF, which was associated with intermittent infection and early clinical alterations, while the exoS+ /exoU− virulotype was associated with chronic infection and worse clinical results. Finally, the presented data highlight the relevance of T3SS virulence markers in the clinical progression and disease severity in CF patients.
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spelling Sarges, Edilene do Socorro Nascimento FalcãoRodrigues, Yan CorrêaFurlaneto, Ismari PeriniMelo, Marcos Vinicios Hino deBrabo, Giulia Leão da CunhaLopes, Kátia Cilene MachadoQuaresma, Ana Judith Pires GarciaLima, Luana Nepomuceno Gondim CostaLima, Karla Valéria Batista2020-11-03T14:59:14Z2020-11-03T14:59:14Z2020SARGES, Edilene do Socorro Nascimento Falcão et al. Pseudomonas aeruginosa type III secretion system virulotypes and their association with clinical features of cystic fibrosis patients. Infection and Drug Resistance, v. 13, p. 3771-3781, Oct. 2020.1178-6973https://patua.iec.gov.br/handle/iec/419310.2147/IDR.S273759Purpose: Pseudomonas aeruginosa appears as the main pathogen in cystic fibrosis (CF) involved in recurrent pneumonia and pulmonary exacerbations. The type III secretion system (T3SS) is one of its main determinants of virulence and is associated with poor clinical progression and increased mortality. This study determined the relationship of clinical features of patients with CF and P. aeruginosa T3SS virulotypes. Materials and Methods: From January 2018 to March 2019, P. aeruginosa were isolated from sputum and/or oropharyngeal swabs. T3SS markers (exoS, exoU, exoT and exoY) were detected by PCR. Clinical severity according to Shwachman-Kulckycki score and spirometry data were associated with T3SS virulotypes. Results: A total of 49 patients had positive cultures for P. aeruginosa. T3SS virulence-related markers were detected as follows: exoS 97.9% (n=48), exoU 63.2% (n=31), exoT 95.9% (n=47) and exoY 97.9% (n=48). The prevalence of exoS+/exoU+ virulotype was higher than previously reported in CF settings, being detected in 61.2% of the evaluated isolates, present in 70% of intermittent infections and with a significantly higher frequency in cases of exacerba-tions. The presence of exoU in chronic infection was not associated with poor clinical results. In chronic infections, the exoS+/exoU− virulotype prevailed (77.8%) and was associated to worse clinical results according to the Shwachman-Kulckycki score and spirometric. Conclusion: Our findings revealed a high prevalence of the atypical exoS+ /exoU+ virulo-type among P. aeruginosa isolates from patients with CF, which was associated with intermittent infection and early clinical alterations, while the exoS+ /exoU− virulotype was associated with chronic infection and worse clinical results. Finally, the presented data highlight the relevance of T3SS virulence markers in the clinical progression and disease severity in CF patients.This research was supported by funding from Fundação de Amparo à Pesquisa do Pará/Universidade do Estado do Pará (FAPESPA/UEPA) [Cooperation grant Nº004/2019], Programa Institucional de Bolsas de Iniciação Científica/ Fundação Amazônia Paraense de Amparo à Pesquisa/ Universidade do Estado do Pará (FAPESPA/UEPA) [Cooperation grant Nº001/2017/PPGBPA], Programa Institucional de Bolsas de Iniciação Científica/Fundação Amazônia Paraense de Amparo à Pesquisa/Universidade do Estado do Pará (FAPESPA/UEPA) [Cooperation grant Nº002/2019/Graduação], Programa Institucional de Bolsas de Iniciação Científica/Instituto Evandro Chagas – Conselho Nacional de Desenvolvimento Científico e Tecnológico (PIBIC/IEC/CNPq) and Instituto Evandro Chagas/Ministério da Saúde/Secretaria de Vigilância em Saúde (IEC/MS/SVS).Universidade do Estado do Pará. Programa de Pós-graduação em Biologia Parasitária na Amazônia. Belém, PA, Brazil / Universidade Federal do Pará. Hospital Universitário João de Barros Barreto. Belém, PA, Brazil.Universidade do Estado do Pará. Programa de Pós-graduação em Biologia Parasitária na Amazônia. Belém, PA, Brazil.Centro Universitário do Pará. Mestrado Profissional em Ensino em Saúde – Educação Médica. Belém, PA, Brazil.Universidade do Estado do Pará. Programa de Pós-graduação em Biologia Parasitária na Amazônia. Belém, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Universidade Federal do Pará. Hospital Universitário João de Barros Barreto. Belém, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Universidade do Estado do Pará. Programa de Pós-graduação em Biologia Parasitária na Amazônia. Belém, PA, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Universidade do Estado do Pará. Programa de Pós-graduação em Biologia Parasitária na Amazônia. Belém, PA, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. 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dc.title.pt_BR.fl_str_mv Pseudomonas aeruginosa type III secretion system virulotypes and their association with clinical features of cystic fibrosis patients
title Pseudomonas aeruginosa type III secretion system virulotypes and their association with clinical features of cystic fibrosis patients
spellingShingle Pseudomonas aeruginosa type III secretion system virulotypes and their association with clinical features of cystic fibrosis patients
Sarges, Edilene do Socorro Nascimento Falcão
Fibrose Cística
Pseudomonas aeruginosa / patogenicidade
Infecções por Pseudomonas / microbiologia
Virulência
title_short Pseudomonas aeruginosa type III secretion system virulotypes and their association with clinical features of cystic fibrosis patients
title_full Pseudomonas aeruginosa type III secretion system virulotypes and their association with clinical features of cystic fibrosis patients
title_fullStr Pseudomonas aeruginosa type III secretion system virulotypes and their association with clinical features of cystic fibrosis patients
title_full_unstemmed Pseudomonas aeruginosa type III secretion system virulotypes and their association with clinical features of cystic fibrosis patients
title_sort Pseudomonas aeruginosa type III secretion system virulotypes and their association with clinical features of cystic fibrosis patients
author Sarges, Edilene do Socorro Nascimento Falcão
author_facet Sarges, Edilene do Socorro Nascimento Falcão
Rodrigues, Yan Corrêa
Furlaneto, Ismari Perini
Melo, Marcos Vinicios Hino de
Brabo, Giulia Leão da Cunha
Lopes, Kátia Cilene Machado
Quaresma, Ana Judith Pires Garcia
Lima, Luana Nepomuceno Gondim Costa
Lima, Karla Valéria Batista
author_role author
author2 Rodrigues, Yan Corrêa
Furlaneto, Ismari Perini
Melo, Marcos Vinicios Hino de
Brabo, Giulia Leão da Cunha
Lopes, Kátia Cilene Machado
Quaresma, Ana Judith Pires Garcia
Lima, Luana Nepomuceno Gondim Costa
Lima, Karla Valéria Batista
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Sarges, Edilene do Socorro Nascimento Falcão
Rodrigues, Yan Corrêa
Furlaneto, Ismari Perini
Melo, Marcos Vinicios Hino de
Brabo, Giulia Leão da Cunha
Lopes, Kátia Cilene Machado
Quaresma, Ana Judith Pires Garcia
Lima, Luana Nepomuceno Gondim Costa
Lima, Karla Valéria Batista
dc.subject.decsPrimary.pt_BR.fl_str_mv Fibrose Cística
Pseudomonas aeruginosa / patogenicidade
Infecções por Pseudomonas / microbiologia
Virulência
topic Fibrose Cística
Pseudomonas aeruginosa / patogenicidade
Infecções por Pseudomonas / microbiologia
Virulência
description Purpose: Pseudomonas aeruginosa appears as the main pathogen in cystic fibrosis (CF) involved in recurrent pneumonia and pulmonary exacerbations. The type III secretion system (T3SS) is one of its main determinants of virulence and is associated with poor clinical progression and increased mortality. This study determined the relationship of clinical features of patients with CF and P. aeruginosa T3SS virulotypes. Materials and Methods: From January 2018 to March 2019, P. aeruginosa were isolated from sputum and/or oropharyngeal swabs. T3SS markers (exoS, exoU, exoT and exoY) were detected by PCR. Clinical severity according to Shwachman-Kulckycki score and spirometry data were associated with T3SS virulotypes. Results: A total of 49 patients had positive cultures for P. aeruginosa. T3SS virulence-related markers were detected as follows: exoS 97.9% (n=48), exoU 63.2% (n=31), exoT 95.9% (n=47) and exoY 97.9% (n=48). The prevalence of exoS+/exoU+ virulotype was higher than previously reported in CF settings, being detected in 61.2% of the evaluated isolates, present in 70% of intermittent infections and with a significantly higher frequency in cases of exacerba-tions. The presence of exoU in chronic infection was not associated with poor clinical results. In chronic infections, the exoS+/exoU− virulotype prevailed (77.8%) and was associated to worse clinical results according to the Shwachman-Kulckycki score and spirometric. Conclusion: Our findings revealed a high prevalence of the atypical exoS+ /exoU+ virulo-type among P. aeruginosa isolates from patients with CF, which was associated with intermittent infection and early clinical alterations, while the exoS+ /exoU− virulotype was associated with chronic infection and worse clinical results. Finally, the presented data highlight the relevance of T3SS virulence markers in the clinical progression and disease severity in CF patients.
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-11-03T14:59:14Z
dc.date.available.fl_str_mv 2020-11-03T14:59:14Z
dc.date.issued.fl_str_mv 2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SARGES, Edilene do Socorro Nascimento Falcão et al. Pseudomonas aeruginosa type III secretion system virulotypes and their association with clinical features of cystic fibrosis patients. Infection and Drug Resistance, v. 13, p. 3771-3781, Oct. 2020.
dc.identifier.uri.fl_str_mv https://patua.iec.gov.br/handle/iec/4193
dc.identifier.issn.-.fl_str_mv 1178-6973
dc.identifier.doi.-.fl_str_mv 10.2147/IDR.S273759
identifier_str_mv SARGES, Edilene do Socorro Nascimento Falcão et al. Pseudomonas aeruginosa type III secretion system virulotypes and their association with clinical features of cystic fibrosis patients. Infection and Drug Resistance, v. 13, p. 3771-3781, Oct. 2020.
1178-6973
10.2147/IDR.S273759
url https://patua.iec.gov.br/handle/iec/4193
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Dove Medical Press
publisher.none.fl_str_mv Dove Medical Press
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