Seizures during pregnancy modify the development of hippocampal interneurons of the offspring
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/443 |
Resumo: | We investigated the effect of epileptic seizures during pregnancy on hippocampal expression of calciumbindingproteins in the offspring. Female Wistar rats were submitted to the pilocarpine model and mated during the chronic period. Seizure frequency was monitored over the entire pregnancy. Pups were perfusedat postnatal days 6 and 13, and the brains processed for Nissl staining and immunohistochemistry for NeuN,calbindin, calretinin, and parvalbumin. Number of stained cells in the hippocampus was estimated throughstereological methods. Our results showed a decrease in epileptic seizure frequency during pregnancy. Nodifferences were observed in NeuN-positive, CR-positive cells, and Nissl-stained hippocampal neuronsbetween the groups. However, there was a significant decrease in calbindin-positive cells (P=0.005) and asignificant increase in parvalbumin-positive cells (P=0.02) in the experimental group when compared withthe control group. These results suggest that seizures during pregnancy affect the development of specifichippocampal interneurons of the offspring. |
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Vale, Tiago Gurgel doLima, Daiana CorreiaLima, Eliângela deTorres, Laila BritoCossa, Ana CarolinaCavalheiro, Esper AbrãoNaffah-Mazzacoratti, Maria da GraçaOliveira, Elaine Menezes deAmado, DéboraSilva, Alexandre Valotta daCabral, Francisco Romero2016-01-26T11:27:48Z2010VALE, Tiago Gurgel do et al. Seizures during pregnancy modify the development of hippocampal interneurons of the offspring. Epilepsy & Behavior, v. 19, n. 1, p. 20-25, 2010.1525-5050https://patua.iec.gov.br/handle/iec/44310.1016/j.yebeh.2010.06.032We investigated the effect of epileptic seizures during pregnancy on hippocampal expression of calciumbindingproteins in the offspring. Female Wistar rats were submitted to the pilocarpine model and mated during the chronic period. Seizure frequency was monitored over the entire pregnancy. Pups were perfusedat postnatal days 6 and 13, and the brains processed for Nissl staining and immunohistochemistry for NeuN,calbindin, calretinin, and parvalbumin. Number of stained cells in the hippocampus was estimated throughstereological methods. Our results showed a decrease in epileptic seizure frequency during pregnancy. Nodifferences were observed in NeuN-positive, CR-positive cells, and Nissl-stained hippocampal neuronsbetween the groups. However, there was a significant decrease in calbindin-positive cells (P=0.005) and asignificant increase in parvalbumin-positive cells (P=0.02) in the experimental group when compared withthe control group. These results suggest that seizures during pregnancy affect the development of specifichippocampal interneurons of the offspring.Universidade Federal de São Paulo. Disciplina de Neurologia Experimental. São Paulo, SP, Brasil.Universidade Federal de São Paulo. Campus Baixada Santista. Departamento de Biociências. Mathias-Santos, SP, Brasil.Universidade Federal de São Paulo. Disciplina de Neurologia Experimental. São Paulo, SP, Brasil.Universidade Federal de São Paulo. Disciplina de Neurologia Experimental. São Paulo, SP, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Belém, PA, Brasil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Centro Nacional de Primatas. Belém, PA, Brasil / Universidade Federal de São Paulo. Disciplina de Neurologia Experimental. São Paulo, SP, Brasil.Universidade Federal de São Paulo. Disciplina de Neurologia Experimental. São Paulo, SP, Brasil.Universidade Federal de São Paulo. Disciplina de Neurologia Experimental. São Paulo, SP, Brasil.Instituto de Ensino e Pesquisa Hospital Albert Einstein. Instituto do Cérebro. São Paulo, SP, Brasil.Universidade Federal de São Paulo. Disciplina de Neurologia Experimental. São Paulo, SP, Brasil.Universidade Federal de São Paulo. Disciplina de Neurologia Experimental. São Paulo, SP, Brasil / Universidade Federal de São Paulo. Campus Baixada Santista. Departamento de Biociências. Mathias-Santos, SP, Brasil.Universidade Federal de São Paulo. Disciplina de Neurologia Experimental. São Paulo, SP, Brasil.application/pdfengElsevierSeizures during pregnancy modify the development of hippocampal interneurons of the offspringinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleEpilepsiaRatosHipocampoinfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALSeizures during pregnancy modify the development of hippocampal interneurons of the offspring .pdfapplication/pdf667618https://patua.iec.gov.br/bitstreams/3e390a2e-4cab-465c-9f5c-437fd167b9ba/downloadef8fb5f6161947fe01ef7542130abc4cMD51TEXTfile_1.pdf.txtfile_1.pdf.txtExtracted texttext/plain37860https://patua.iec.gov.br/bitstreams/afbd606b-6bb7-4a4e-814b-3dfca4e77508/download3d97f09cf076b9030d5475e51982270cMD52Seizures during pregnancy modify the development of hippocampal interneurons of the offspring .pdf.txtSeizures during pregnancy modify the development of hippocampal interneurons of the offspring .pdf.txtExtracted texttext/plain38731https://patua.iec.gov.br/bitstreams/82f2989d-0081-48a5-a109-d568b55d34dc/downloadfd084289ba2a583376148d18dd54c68fMD57THUMBNAILfile_1.pdf.jpgfile_1.pdf.jpgIM Thumbnailimage/jpeg6446https://patua.iec.gov.br/bitstreams/7f87a158-e193-4e0f-b8ad-cd3324d17ebc/download40f3d2f284a9a86d618daf21a8e5c54aMD53Seizures during pregnancy modify the development of hippocampal interneurons of the offspring .pdf.jpgSeizures during pregnancy modify the development of hippocampal interneurons of the offspring .pdf.jpgGenerated Thumbnailimage/jpeg5877https://patua.iec.gov.br/bitstreams/888547fa-e1fc-4654-a7b1-c8915dcf2797/downloade0063c42b8752852a61e507f9712140cMD58LICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv |
Seizures during pregnancy modify the development of hippocampal interneurons of the offspring |
title |
Seizures during pregnancy modify the development of hippocampal interneurons of the offspring |
spellingShingle |
Seizures during pregnancy modify the development of hippocampal interneurons of the offspring Vale, Tiago Gurgel do Epilepsia Ratos Hipocampo |
title_short |
Seizures during pregnancy modify the development of hippocampal interneurons of the offspring |
title_full |
Seizures during pregnancy modify the development of hippocampal interneurons of the offspring |
title_fullStr |
Seizures during pregnancy modify the development of hippocampal interneurons of the offspring |
title_full_unstemmed |
Seizures during pregnancy modify the development of hippocampal interneurons of the offspring |
title_sort |
Seizures during pregnancy modify the development of hippocampal interneurons of the offspring |
author |
Vale, Tiago Gurgel do |
author_facet |
Vale, Tiago Gurgel do Lima, Daiana Correia Lima, Eliângela de Torres, Laila Brito Cossa, Ana Carolina Cavalheiro, Esper Abrão Naffah-Mazzacoratti, Maria da Graça Oliveira, Elaine Menezes de Amado, Débora Silva, Alexandre Valotta da Cabral, Francisco Romero |
author_role |
author |
author2 |
Lima, Daiana Correia Lima, Eliângela de Torres, Laila Brito Cossa, Ana Carolina Cavalheiro, Esper Abrão Naffah-Mazzacoratti, Maria da Graça Oliveira, Elaine Menezes de Amado, Débora Silva, Alexandre Valotta da Cabral, Francisco Romero |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Vale, Tiago Gurgel do Lima, Daiana Correia Lima, Eliângela de Torres, Laila Brito Cossa, Ana Carolina Cavalheiro, Esper Abrão Naffah-Mazzacoratti, Maria da Graça Oliveira, Elaine Menezes de Amado, Débora Silva, Alexandre Valotta da Cabral, Francisco Romero |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Epilepsia Ratos Hipocampo |
topic |
Epilepsia Ratos Hipocampo |
description |
We investigated the effect of epileptic seizures during pregnancy on hippocampal expression of calciumbindingproteins in the offspring. Female Wistar rats were submitted to the pilocarpine model and mated during the chronic period. Seizure frequency was monitored over the entire pregnancy. Pups were perfusedat postnatal days 6 and 13, and the brains processed for Nissl staining and immunohistochemistry for NeuN,calbindin, calretinin, and parvalbumin. Number of stained cells in the hippocampus was estimated throughstereological methods. Our results showed a decrease in epileptic seizure frequency during pregnancy. Nodifferences were observed in NeuN-positive, CR-positive cells, and Nissl-stained hippocampal neuronsbetween the groups. However, there was a significant decrease in calbindin-positive cells (P=0.005) and asignificant increase in parvalbumin-positive cells (P=0.02) in the experimental group when compared withthe control group. These results suggest that seizures during pregnancy affect the development of specifichippocampal interneurons of the offspring. |
publishDate |
2010 |
dc.date.issued.fl_str_mv |
2010 |
dc.date.accessioned.fl_str_mv |
2016-01-26T11:27:48Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
VALE, Tiago Gurgel do et al. Seizures during pregnancy modify the development of hippocampal interneurons of the offspring. Epilepsy & Behavior, v. 19, n. 1, p. 20-25, 2010. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/443 |
dc.identifier.issn.-.fl_str_mv |
1525-5050 |
dc.identifier.doi.-.fl_str_mv |
10.1016/j.yebeh.2010.06.032 |
identifier_str_mv |
VALE, Tiago Gurgel do et al. Seizures during pregnancy modify the development of hippocampal interneurons of the offspring. Epilepsy & Behavior, v. 19, n. 1, p. 20-25, 2010. 1525-5050 10.1016/j.yebeh.2010.06.032 |
url |
https://patua.iec.gov.br/handle/iec/443 |
dc.language.iso.fl_str_mv |
eng |
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eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
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reponame:Repositório Digital do Instituto Evandro Chagas (Patuá) instname:Instituto Evandro Chagas (IEC) instacron:IEC |
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Instituto Evandro Chagas (IEC) |
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IEC |
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IEC |
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Repositório Digital do Instituto Evandro Chagas (Patuá) |
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Repositório Digital do Instituto Evandro Chagas (Patuá) |
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