Molecular identification, genotyping, phenotyping, and antifungal susceptibilities of medically important trichosporon, apiotrichum, and cutaneotrichosporon species

Detalhes bibliográficos
Autor(a) principal: Espirito Santo, Elaine Patrícia Tavares do
Data de Publicação: 2019
Outros Autores: Monteiro, Ruan Campos, Costa, Ana Roberta Fusco da, Silva, Silvia Helena Marques da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
Texto Completo: https://patua.iec.gov.br/handle/iec/3995
Resumo: Recently, Trichosporon taxonomy has been reevaluated and new genera of the Trichosporonaceae family have been described. Here, 26 clinical isolates were submitted for identification via sequencing of the intergenic space 1 (IGS1) region, genotyping, and investigation of virulence factors. Antifungal susceptibility was determined using the CLSI broth microdilution method for fluconazole (FLC), itraconazole (ITC), and amphotericin B (AMB). Of these, 24 isolates were identified, including 12 T. asahii, 4 T. inkin, 3 T. faecale, 1 T. coremiiforme, 1 T. japonicum, 2 Cutaneotrichosporon dermatis (formerly T. dermatis), and 1 Apiotrichum mycotoxinivorans (formerly T. mycotoxinivorans). Species-level identification of 2 isolates was not successful; they were described as Trichosporon sp. We observed optimal colonial development at 35-40 °C. Lipase was the major extracellular enzyme produced (100%); caseinase was not produced (0%). Biofilms were produced by all isolates (classified as low). High AMB minimum inhibitory concentration (MIC) was observed, with all strains resistant. Fluconazole was the most active drug among the antifungals tested. However, high MICs for FLC were observed in C. dermatis and A. mycotoxinivorans species, which also showed resistance to ITC and AMB. This study, conducted in the Northern region of Brazil, identified 5 Trichosporon species along with C. dermatis and A. mycotoxinivorans and demonstrated their pathogenic potential through their ability to produce important virulence factors. This may contribute to our understanding of the epidemiology and factors related to the pathogeneses of species in the Trichosporonaceae family.
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spelling Espirito Santo, Elaine Patrícia Tavares doMonteiro, Ruan CamposCosta, Ana Roberta Fusco daSilva, Silvia Helena Marques da2019-12-11T17:52:07Z2019-12-11T17:52:07Z2019ESPIRITO SANTO, Elaine Patrícia Tavares do et al. Molecular identification, genotyping, phenotyping, and antifungal susceptibilities of medically important trichosporon, apiotrichum, and cutaneotrichosporon species. Mycopathologia, v. 185, n. 2, p.307-317, Apr. 2020.1573-0832https://patua.iec.gov.br/handle/iec/399510.1007/s11046-019-00407-xRecently, Trichosporon taxonomy has been reevaluated and new genera of the Trichosporonaceae family have been described. Here, 26 clinical isolates were submitted for identification via sequencing of the intergenic space 1 (IGS1) region, genotyping, and investigation of virulence factors. Antifungal susceptibility was determined using the CLSI broth microdilution method for fluconazole (FLC), itraconazole (ITC), and amphotericin B (AMB). Of these, 24 isolates were identified, including 12 T. asahii, 4 T. inkin, 3 T. faecale, 1 T. coremiiforme, 1 T. japonicum, 2 Cutaneotrichosporon dermatis (formerly T. dermatis), and 1 Apiotrichum mycotoxinivorans (formerly T. mycotoxinivorans). Species-level identification of 2 isolates was not successful; they were described as Trichosporon sp. We observed optimal colonial development at 35-40 °C. Lipase was the major extracellular enzyme produced (100%); caseinase was not produced (0%). Biofilms were produced by all isolates (classified as low). High AMB minimum inhibitory concentration (MIC) was observed, with all strains resistant. Fluconazole was the most active drug among the antifungals tested. However, high MICs for FLC were observed in C. dermatis and A. mycotoxinivorans species, which also showed resistance to ITC and AMB. This study, conducted in the Northern region of Brazil, identified 5 Trichosporon species along with C. dermatis and A. mycotoxinivorans and demonstrated their pathogenic potential through their ability to produce important virulence factors. This may contribute to our understanding of the epidemiology and factors related to the pathogeneses of species in the Trichosporonaceae family.This study was funded by the Evandro Chagas Institute.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Micologia. Ananindeua, PA, Brasil / Federal University of Pará. Biological Sciences Center. Postgraduate Program in Biology of Infectious and Parasitic Agents. Belém, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Micologia. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Micologia. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Micologia. Ananindeua, PA, Brasil / Federal University of Pará. Biological Sciences Center. Postgraduate Program in Biology of Infectious and Parasitic Agents. Belém, PA, Brazil.engSpringer VerlagMolecular identification, genotyping, phenotyping, and antifungal susceptibilities of medically important trichosporon, apiotrichum, and cutaneotrichosporon speciesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleTrichosporon / classificaçãoTrichosporon / isolamento & purificaçãoFungos MitospóricosAntifúngicosFatores de Virulênciainfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECLICENSElicense.txtlicense.txttext/plain; charset=utf-82182https://patua.iec.gov.br/bitstreams/c6710d3a-f4f9-4091-8b56-f70aa69eafcf/download11832eea31b16df8613079d742d61793MD52TEXTMolecular identification, genotyping, phenotyping, and antifungal susceptibilities of medically important trichosporon, apiotrichum, and cutaneotrichosporon species.pdf.txtMolecular identification, genotyping, phenotyping, and antifungal susceptibilities of medically important trichosporon, apiotrichum, and cutaneotrichosporon species.pdf.txtExtracted texttext/plain43941https://patua.iec.gov.br/bitstreams/fa14d884-26c9-464b-aef6-89bd2521d4d6/download6dbaf04257ccc3b0356ca935e6401d90MD56THUMBNAILMolecular identification, genotyping, phenotyping, and antifungal susceptibilities of medically important trichosporon, apiotrichum, and cutaneotrichosporon species.pdf.jpgMolecular identification, genotyping, phenotyping, and antifungal susceptibilities of medically important trichosporon, apiotrichum, and cutaneotrichosporon species.pdf.jpgGenerated Thumbnailimage/jpeg5095https://patua.iec.gov.br/bitstreams/d04a6980-029b-4433-8b9a-090867f802e2/download7c7e31b3d167d1bb7ec4918a973d9cf7MD57ORIGINALMolecular identification, genotyping, phenotyping, and antifungal susceptibilities of medically important trichosporon, apiotrichum, and cutaneotrichosporon species.pdfMolecular identification, genotyping, phenotyping, and antifungal susceptibilities of medically important trichosporon, apiotrichum, and cutaneotrichosporon species.pdfapplication/pdf570113https://patua.iec.gov.br/bitstreams/761c70b7-b53f-4997-92d8-5ca81e07d108/download2140a8d0651ac233124e6b53ac44548eMD55iec/39952022-10-20 21:06:37.629oai:patua.iec.gov.br:iec/3995https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-20T21:06:37Repositório Digital do Instituto Evandro Chagas (Patuá) - 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dc.title.pt_BR.fl_str_mv Molecular identification, genotyping, phenotyping, and antifungal susceptibilities of medically important trichosporon, apiotrichum, and cutaneotrichosporon species
title Molecular identification, genotyping, phenotyping, and antifungal susceptibilities of medically important trichosporon, apiotrichum, and cutaneotrichosporon species
spellingShingle Molecular identification, genotyping, phenotyping, and antifungal susceptibilities of medically important trichosporon, apiotrichum, and cutaneotrichosporon species
Espirito Santo, Elaine Patrícia Tavares do
Trichosporon / classificação
Trichosporon / isolamento & purificação
Fungos Mitospóricos
Antifúngicos
Fatores de Virulência
title_short Molecular identification, genotyping, phenotyping, and antifungal susceptibilities of medically important trichosporon, apiotrichum, and cutaneotrichosporon species
title_full Molecular identification, genotyping, phenotyping, and antifungal susceptibilities of medically important trichosporon, apiotrichum, and cutaneotrichosporon species
title_fullStr Molecular identification, genotyping, phenotyping, and antifungal susceptibilities of medically important trichosporon, apiotrichum, and cutaneotrichosporon species
title_full_unstemmed Molecular identification, genotyping, phenotyping, and antifungal susceptibilities of medically important trichosporon, apiotrichum, and cutaneotrichosporon species
title_sort Molecular identification, genotyping, phenotyping, and antifungal susceptibilities of medically important trichosporon, apiotrichum, and cutaneotrichosporon species
author Espirito Santo, Elaine Patrícia Tavares do
author_facet Espirito Santo, Elaine Patrícia Tavares do
Monteiro, Ruan Campos
Costa, Ana Roberta Fusco da
Silva, Silvia Helena Marques da
author_role author
author2 Monteiro, Ruan Campos
Costa, Ana Roberta Fusco da
Silva, Silvia Helena Marques da
author2_role author
author
author
dc.contributor.author.fl_str_mv Espirito Santo, Elaine Patrícia Tavares do
Monteiro, Ruan Campos
Costa, Ana Roberta Fusco da
Silva, Silvia Helena Marques da
dc.subject.decsPrimary.pt_BR.fl_str_mv Trichosporon / classificação
Trichosporon / isolamento & purificação
Fungos Mitospóricos
Antifúngicos
Fatores de Virulência
topic Trichosporon / classificação
Trichosporon / isolamento & purificação
Fungos Mitospóricos
Antifúngicos
Fatores de Virulência
description Recently, Trichosporon taxonomy has been reevaluated and new genera of the Trichosporonaceae family have been described. Here, 26 clinical isolates were submitted for identification via sequencing of the intergenic space 1 (IGS1) region, genotyping, and investigation of virulence factors. Antifungal susceptibility was determined using the CLSI broth microdilution method for fluconazole (FLC), itraconazole (ITC), and amphotericin B (AMB). Of these, 24 isolates were identified, including 12 T. asahii, 4 T. inkin, 3 T. faecale, 1 T. coremiiforme, 1 T. japonicum, 2 Cutaneotrichosporon dermatis (formerly T. dermatis), and 1 Apiotrichum mycotoxinivorans (formerly T. mycotoxinivorans). Species-level identification of 2 isolates was not successful; they were described as Trichosporon sp. We observed optimal colonial development at 35-40 °C. Lipase was the major extracellular enzyme produced (100%); caseinase was not produced (0%). Biofilms were produced by all isolates (classified as low). High AMB minimum inhibitory concentration (MIC) was observed, with all strains resistant. Fluconazole was the most active drug among the antifungals tested. However, high MICs for FLC were observed in C. dermatis and A. mycotoxinivorans species, which also showed resistance to ITC and AMB. This study, conducted in the Northern region of Brazil, identified 5 Trichosporon species along with C. dermatis and A. mycotoxinivorans and demonstrated their pathogenic potential through their ability to produce important virulence factors. This may contribute to our understanding of the epidemiology and factors related to the pathogeneses of species in the Trichosporonaceae family.
publishDate 2019
dc.date.accessioned.fl_str_mv 2019-12-11T17:52:07Z
dc.date.available.fl_str_mv 2019-12-11T17:52:07Z
dc.date.issued.fl_str_mv 2019
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv ESPIRITO SANTO, Elaine Patrícia Tavares do et al. Molecular identification, genotyping, phenotyping, and antifungal susceptibilities of medically important trichosporon, apiotrichum, and cutaneotrichosporon species. Mycopathologia, v. 185, n. 2, p.307-317, Apr. 2020.
dc.identifier.uri.fl_str_mv https://patua.iec.gov.br/handle/iec/3995
dc.identifier.issn.-.fl_str_mv 1573-0832
dc.identifier.doi.-.fl_str_mv 10.1007/s11046-019-00407-x
identifier_str_mv ESPIRITO SANTO, Elaine Patrícia Tavares do et al. Molecular identification, genotyping, phenotyping, and antifungal susceptibilities of medically important trichosporon, apiotrichum, and cutaneotrichosporon species. Mycopathologia, v. 185, n. 2, p.307-317, Apr. 2020.
1573-0832
10.1007/s11046-019-00407-x
url https://patua.iec.gov.br/handle/iec/3995
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