Molecular identification, genotyping, phenotyping, and antifungal susceptibilities of medically important trichosporon, apiotrichum, and cutaneotrichosporon species
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/3995 |
Resumo: | Recently, Trichosporon taxonomy has been reevaluated and new genera of the Trichosporonaceae family have been described. Here, 26 clinical isolates were submitted for identification via sequencing of the intergenic space 1 (IGS1) region, genotyping, and investigation of virulence factors. Antifungal susceptibility was determined using the CLSI broth microdilution method for fluconazole (FLC), itraconazole (ITC), and amphotericin B (AMB). Of these, 24 isolates were identified, including 12 T. asahii, 4 T. inkin, 3 T. faecale, 1 T. coremiiforme, 1 T. japonicum, 2 Cutaneotrichosporon dermatis (formerly T. dermatis), and 1 Apiotrichum mycotoxinivorans (formerly T. mycotoxinivorans). Species-level identification of 2 isolates was not successful; they were described as Trichosporon sp. We observed optimal colonial development at 35-40 °C. Lipase was the major extracellular enzyme produced (100%); caseinase was not produced (0%). Biofilms were produced by all isolates (classified as low). High AMB minimum inhibitory concentration (MIC) was observed, with all strains resistant. Fluconazole was the most active drug among the antifungals tested. However, high MICs for FLC were observed in C. dermatis and A. mycotoxinivorans species, which also showed resistance to ITC and AMB. This study, conducted in the Northern region of Brazil, identified 5 Trichosporon species along with C. dermatis and A. mycotoxinivorans and demonstrated their pathogenic potential through their ability to produce important virulence factors. This may contribute to our understanding of the epidemiology and factors related to the pathogeneses of species in the Trichosporonaceae family. |
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Espirito Santo, Elaine Patrícia Tavares doMonteiro, Ruan CamposCosta, Ana Roberta Fusco daSilva, Silvia Helena Marques da2019-12-11T17:52:07Z2019-12-11T17:52:07Z2019ESPIRITO SANTO, Elaine Patrícia Tavares do et al. Molecular identification, genotyping, phenotyping, and antifungal susceptibilities of medically important trichosporon, apiotrichum, and cutaneotrichosporon species. Mycopathologia, v. 185, n. 2, p.307-317, Apr. 2020.1573-0832https://patua.iec.gov.br/handle/iec/399510.1007/s11046-019-00407-xRecently, Trichosporon taxonomy has been reevaluated and new genera of the Trichosporonaceae family have been described. Here, 26 clinical isolates were submitted for identification via sequencing of the intergenic space 1 (IGS1) region, genotyping, and investigation of virulence factors. Antifungal susceptibility was determined using the CLSI broth microdilution method for fluconazole (FLC), itraconazole (ITC), and amphotericin B (AMB). Of these, 24 isolates were identified, including 12 T. asahii, 4 T. inkin, 3 T. faecale, 1 T. coremiiforme, 1 T. japonicum, 2 Cutaneotrichosporon dermatis (formerly T. dermatis), and 1 Apiotrichum mycotoxinivorans (formerly T. mycotoxinivorans). Species-level identification of 2 isolates was not successful; they were described as Trichosporon sp. We observed optimal colonial development at 35-40 °C. Lipase was the major extracellular enzyme produced (100%); caseinase was not produced (0%). Biofilms were produced by all isolates (classified as low). High AMB minimum inhibitory concentration (MIC) was observed, with all strains resistant. Fluconazole was the most active drug among the antifungals tested. However, high MICs for FLC were observed in C. dermatis and A. mycotoxinivorans species, which also showed resistance to ITC and AMB. This study, conducted in the Northern region of Brazil, identified 5 Trichosporon species along with C. dermatis and A. mycotoxinivorans and demonstrated their pathogenic potential through their ability to produce important virulence factors. This may contribute to our understanding of the epidemiology and factors related to the pathogeneses of species in the Trichosporonaceae family.This study was funded by the Evandro Chagas Institute.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Micologia. Ananindeua, PA, Brasil / Federal University of Pará. Biological Sciences Center. Postgraduate Program in Biology of Infectious and Parasitic Agents. Belém, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Micologia. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Micologia. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Micologia. Ananindeua, PA, Brasil / Federal University of Pará. Biological Sciences Center. Postgraduate Program in Biology of Infectious and Parasitic Agents. Belém, PA, Brazil.engSpringer VerlagMolecular identification, genotyping, phenotyping, and antifungal susceptibilities of medically important trichosporon, apiotrichum, and cutaneotrichosporon speciesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleTrichosporon / classificaçãoTrichosporon / isolamento & purificaçãoFungos MitospóricosAntifúngicosFatores de Virulênciainfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECLICENSElicense.txtlicense.txttext/plain; charset=utf-82182https://patua.iec.gov.br/bitstreams/c6710d3a-f4f9-4091-8b56-f70aa69eafcf/download11832eea31b16df8613079d742d61793MD52TEXTMolecular identification, genotyping, phenotyping, and antifungal susceptibilities of medically important trichosporon, apiotrichum, and cutaneotrichosporon species.pdf.txtMolecular identification, genotyping, phenotyping, and antifungal susceptibilities of medically important trichosporon, apiotrichum, and cutaneotrichosporon species.pdf.txtExtracted texttext/plain43941https://patua.iec.gov.br/bitstreams/fa14d884-26c9-464b-aef6-89bd2521d4d6/download6dbaf04257ccc3b0356ca935e6401d90MD56THUMBNAILMolecular identification, genotyping, phenotyping, and antifungal susceptibilities of medically important trichosporon, apiotrichum, and cutaneotrichosporon species.pdf.jpgMolecular identification, genotyping, phenotyping, and antifungal susceptibilities of medically important trichosporon, apiotrichum, and cutaneotrichosporon species.pdf.jpgGenerated Thumbnailimage/jpeg5095https://patua.iec.gov.br/bitstreams/d04a6980-029b-4433-8b9a-090867f802e2/download7c7e31b3d167d1bb7ec4918a973d9cf7MD57ORIGINALMolecular identification, genotyping, phenotyping, and antifungal susceptibilities of medically important trichosporon, apiotrichum, and cutaneotrichosporon species.pdfMolecular identification, genotyping, phenotyping, and antifungal susceptibilities of medically important trichosporon, apiotrichum, and cutaneotrichosporon species.pdfapplication/pdf570113https://patua.iec.gov.br/bitstreams/761c70b7-b53f-4997-92d8-5ca81e07d108/download2140a8d0651ac233124e6b53ac44548eMD55iec/39952022-10-20 21:06:37.629oai:patua.iec.gov.br:iec/3995https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-20T21:06:37Repositório Digital do Instituto Evandro Chagas (Patuá) - 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dc.title.pt_BR.fl_str_mv |
Molecular identification, genotyping, phenotyping, and antifungal susceptibilities of medically important trichosporon, apiotrichum, and cutaneotrichosporon species |
title |
Molecular identification, genotyping, phenotyping, and antifungal susceptibilities of medically important trichosporon, apiotrichum, and cutaneotrichosporon species |
spellingShingle |
Molecular identification, genotyping, phenotyping, and antifungal susceptibilities of medically important trichosporon, apiotrichum, and cutaneotrichosporon species Espirito Santo, Elaine Patrícia Tavares do Trichosporon / classificação Trichosporon / isolamento & purificação Fungos Mitospóricos Antifúngicos Fatores de Virulência |
title_short |
Molecular identification, genotyping, phenotyping, and antifungal susceptibilities of medically important trichosporon, apiotrichum, and cutaneotrichosporon species |
title_full |
Molecular identification, genotyping, phenotyping, and antifungal susceptibilities of medically important trichosporon, apiotrichum, and cutaneotrichosporon species |
title_fullStr |
Molecular identification, genotyping, phenotyping, and antifungal susceptibilities of medically important trichosporon, apiotrichum, and cutaneotrichosporon species |
title_full_unstemmed |
Molecular identification, genotyping, phenotyping, and antifungal susceptibilities of medically important trichosporon, apiotrichum, and cutaneotrichosporon species |
title_sort |
Molecular identification, genotyping, phenotyping, and antifungal susceptibilities of medically important trichosporon, apiotrichum, and cutaneotrichosporon species |
author |
Espirito Santo, Elaine Patrícia Tavares do |
author_facet |
Espirito Santo, Elaine Patrícia Tavares do Monteiro, Ruan Campos Costa, Ana Roberta Fusco da Silva, Silvia Helena Marques da |
author_role |
author |
author2 |
Monteiro, Ruan Campos Costa, Ana Roberta Fusco da Silva, Silvia Helena Marques da |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Espirito Santo, Elaine Patrícia Tavares do Monteiro, Ruan Campos Costa, Ana Roberta Fusco da Silva, Silvia Helena Marques da |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Trichosporon / classificação Trichosporon / isolamento & purificação Fungos Mitospóricos Antifúngicos Fatores de Virulência |
topic |
Trichosporon / classificação Trichosporon / isolamento & purificação Fungos Mitospóricos Antifúngicos Fatores de Virulência |
description |
Recently, Trichosporon taxonomy has been reevaluated and new genera of the Trichosporonaceae family have been described. Here, 26 clinical isolates were submitted for identification via sequencing of the intergenic space 1 (IGS1) region, genotyping, and investigation of virulence factors. Antifungal susceptibility was determined using the CLSI broth microdilution method for fluconazole (FLC), itraconazole (ITC), and amphotericin B (AMB). Of these, 24 isolates were identified, including 12 T. asahii, 4 T. inkin, 3 T. faecale, 1 T. coremiiforme, 1 T. japonicum, 2 Cutaneotrichosporon dermatis (formerly T. dermatis), and 1 Apiotrichum mycotoxinivorans (formerly T. mycotoxinivorans). Species-level identification of 2 isolates was not successful; they were described as Trichosporon sp. We observed optimal colonial development at 35-40 °C. Lipase was the major extracellular enzyme produced (100%); caseinase was not produced (0%). Biofilms were produced by all isolates (classified as low). High AMB minimum inhibitory concentration (MIC) was observed, with all strains resistant. Fluconazole was the most active drug among the antifungals tested. However, high MICs for FLC were observed in C. dermatis and A. mycotoxinivorans species, which also showed resistance to ITC and AMB. This study, conducted in the Northern region of Brazil, identified 5 Trichosporon species along with C. dermatis and A. mycotoxinivorans and demonstrated their pathogenic potential through their ability to produce important virulence factors. This may contribute to our understanding of the epidemiology and factors related to the pathogeneses of species in the Trichosporonaceae family. |
publishDate |
2019 |
dc.date.accessioned.fl_str_mv |
2019-12-11T17:52:07Z |
dc.date.available.fl_str_mv |
2019-12-11T17:52:07Z |
dc.date.issued.fl_str_mv |
2019 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
ESPIRITO SANTO, Elaine Patrícia Tavares do et al. Molecular identification, genotyping, phenotyping, and antifungal susceptibilities of medically important trichosporon, apiotrichum, and cutaneotrichosporon species. Mycopathologia, v. 185, n. 2, p.307-317, Apr. 2020. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/3995 |
dc.identifier.issn.-.fl_str_mv |
1573-0832 |
dc.identifier.doi.-.fl_str_mv |
10.1007/s11046-019-00407-x |
identifier_str_mv |
ESPIRITO SANTO, Elaine Patrícia Tavares do et al. Molecular identification, genotyping, phenotyping, and antifungal susceptibilities of medically important trichosporon, apiotrichum, and cutaneotrichosporon species. Mycopathologia, v. 185, n. 2, p.307-317, Apr. 2020. 1573-0832 10.1007/s11046-019-00407-x |
url |
https://patua.iec.gov.br/handle/iec/3995 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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Springer Verlag |
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Springer Verlag |
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