Cytogenetic, growth rate and virus susceptibility differences in sublines of H. Ep. 2 cells

Detalhes bibliográficos
Autor(a) principal: Mallmann, M. Clara
Data de Publicação: 1970
Outros Autores: Pinheiro, Francisco de Paula, Salzano, F. M, Ayres, M
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
Texto Completo: https://patua.iec.gov.br/handle/iec/969
Resumo: Two sublines of H. Ep. 2, obtained from New Haven (NH) and São Paulo (SP) respectively, were studied in relation to their morphology, growth rate, virus susceptibility and chromosomes. The São Paulo subline was divided into two, one cultivated in Hanks lactalbumin medium plus 20% of calf serum and the other in modified Eagle's medium, to investigate the possible influence of the medium of cultivation on these variables. This influence proved to be small. But significant differences were observed between the two sublines, H. Ep. 2 NH showing a lowered rate of growth, less susceptibility to virus infections and a higher modal chromosome number. The changes in chromosome number were of such a nature that there was practically no overlapping in the range of variation in the two sublines. Groups B, D and F were the most affected.
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spelling Mallmann, M. ClaraPinheiro, Francisco de PaulaSalzano, F. MAyres, M2016-01-26T11:39:52Z1970MALLMANN, M. Clara et al. Cytogenetic, growth rate and virus susceptibility differences in sublines of H. Ep. 2 cells. Cytologia, v. 35, n. 2, p. 262-270, 1970.0011-4545https://patua.iec.gov.br/handle/iec/969Two sublines of H. Ep. 2, obtained from New Haven (NH) and São Paulo (SP) respectively, were studied in relation to their morphology, growth rate, virus susceptibility and chromosomes. The São Paulo subline was divided into two, one cultivated in Hanks lactalbumin medium plus 20% of calf serum and the other in modified Eagle's medium, to investigate the possible influence of the medium of cultivation on these variables. This influence proved to be small. But significant differences were observed between the two sublines, H. Ep. 2 NH showing a lowered rate of growth, less susceptibility to virus infections and a higher modal chromosome number. The changes in chromosome number were of such a nature that there was practically no overlapping in the range of variation in the two sublines. Groups B, D and F were the most affected.Universidade Federal do Rio Grande do Sul. Instituto de Ciências Naturais. Departamento de Genética. Porto Alegre, RS, Brasil.Ministério da Saúde. Fundação Serviço Especial de Saúde Pública. Instituto Evandro Chagas. Belém, PA, Brasil.Universidade Federal do Pará. Faculdade de Filosofia, Ciências e Letras. Laboratório de Genética. Belém, PA, Brasil.Universidade Federal do Pará. Faculdade de Filosofia, Ciências e Letras. Laboratório de Genética. Belém, PA, Brasil.application/pdfengInternational Association of CytologyCytogenetic, growth rate and virus susceptibility differences in sublines of H. 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dc.title.pt_BR.fl_str_mv Cytogenetic, growth rate and virus susceptibility differences in sublines of H. Ep. 2 cells
title Cytogenetic, growth rate and virus susceptibility differences in sublines of H. Ep. 2 cells
spellingShingle Cytogenetic, growth rate and virus susceptibility differences in sublines of H. Ep. 2 cells
Mallmann, M. Clara
Carcinoma
Dermatomicoses / imunologia
Modelos Animais de Doenças
Quelóide
title_short Cytogenetic, growth rate and virus susceptibility differences in sublines of H. Ep. 2 cells
title_full Cytogenetic, growth rate and virus susceptibility differences in sublines of H. Ep. 2 cells
title_fullStr Cytogenetic, growth rate and virus susceptibility differences in sublines of H. Ep. 2 cells
title_full_unstemmed Cytogenetic, growth rate and virus susceptibility differences in sublines of H. Ep. 2 cells
title_sort Cytogenetic, growth rate and virus susceptibility differences in sublines of H. Ep. 2 cells
author Mallmann, M. Clara
author_facet Mallmann, M. Clara
Pinheiro, Francisco de Paula
Salzano, F. M
Ayres, M
author_role author
author2 Pinheiro, Francisco de Paula
Salzano, F. M
Ayres, M
author2_role author
author
author
dc.contributor.author.fl_str_mv Mallmann, M. Clara
Pinheiro, Francisco de Paula
Salzano, F. M
Ayres, M
dc.subject.decsPrimary.pt_BR.fl_str_mv Carcinoma
Dermatomicoses / imunologia
Modelos Animais de Doenças
Quelóide
topic Carcinoma
Dermatomicoses / imunologia
Modelos Animais de Doenças
Quelóide
description Two sublines of H. Ep. 2, obtained from New Haven (NH) and São Paulo (SP) respectively, were studied in relation to their morphology, growth rate, virus susceptibility and chromosomes. The São Paulo subline was divided into two, one cultivated in Hanks lactalbumin medium plus 20% of calf serum and the other in modified Eagle's medium, to investigate the possible influence of the medium of cultivation on these variables. This influence proved to be small. But significant differences were observed between the two sublines, H. Ep. 2 NH showing a lowered rate of growth, less susceptibility to virus infections and a higher modal chromosome number. The changes in chromosome number were of such a nature that there was practically no overlapping in the range of variation in the two sublines. Groups B, D and F were the most affected.
publishDate 1970
dc.date.issued.fl_str_mv 1970
dc.date.accessioned.fl_str_mv 2016-01-26T11:39:52Z
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dc.identifier.citation.fl_str_mv MALLMANN, M. Clara et al. Cytogenetic, growth rate and virus susceptibility differences in sublines of H. Ep. 2 cells. Cytologia, v. 35, n. 2, p. 262-270, 1970.
dc.identifier.uri.fl_str_mv https://patua.iec.gov.br/handle/iec/969
dc.identifier.issn.-.fl_str_mv 0011-4545
identifier_str_mv MALLMANN, M. Clara et al. Cytogenetic, growth rate and virus susceptibility differences in sublines of H. Ep. 2 cells. Cytologia, v. 35, n. 2, p. 262-270, 1970.
0011-4545
url https://patua.iec.gov.br/handle/iec/969
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dc.publisher.none.fl_str_mv International Association of Cytology
publisher.none.fl_str_mv International Association of Cytology
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