The crab heparin-like compound exhibits a strong inhibitory effect on infections by dengue virus-2

• Autor(a) principal: Almeida, Marlyanne Maria Carvalho Silva de
• Data de Publicação: 2021
• Outros Autores: Almeida Júnior, Renato Ferreira de, Aquino, Ana Alice de, Pinto, Lívia de Lourdes, Machado, Paula Renata Lima, Chavante, Suely Ferreira, Farias, Kleber Juvenal Silva, Andrade, Giulianna Paiva Viana de
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
• Texto Completo: https://patua.iec.gov.br/handle/iec/4294
• topic: Dengue / virologia; Heparina / análise; Células Vero / citologia; Antivirais; Reação em Cadeia da Polimerase em Tempo Real / métodos

Background: According to the World Health Organization (WHO), two-fifths of the world population is at risk of infection by DENV. There are no safe and effective vaccines estab-lished. Sulfated glycosaminoglycans such as heparin, used as anticoagulants, inhibit the initial step of dengue viral replication. Recently, an isolated heparin analogue Goniopsis cruentata (cCTH) has presented a low anticoagulant effect with reduced bleeding risk. Methods: The antiviral activity of cCTH and heparin compounds against DENV-2 in Vero cell culture was determined by quantitative RT-PCR (qRT-PCR) and titration. For this, four trials were carried out: treatment of the cells for 2 h before viral inoculation, concomitant viral inoculation treatment, treatment after viral inoculation and virucidal assay. Subsequently, the culture superna-tants were collected for periods of 24, 48 and 72 h. Results: Our results demonstrated that cCTH and heparin showed antiviral activity against DENV-2. Conclusion: These data suggest that both compounds prevented viral replication in cultured Vero cells.