Activity of alkaloids from Aspidosperma nitidum against Leishmania (Leishmania) amazonensis

• Autor(a) principal: Veiga, Andreza do Socorro Silva da
• Data de Publicação: 2022
• Outros Autores: Silveira, Fernando Tobias, Silva, Edilene Oliveira da, Diniz Júnior, José Antônio Picanço, Araújo, Sanderson Corrêa, Campos, Marliane Batista, Marinho, Andrey Moacir do Rosário, Brandão, Geraldo Célio, Vale, Valdicley Vieira, Percário, Sandro, Dolabela, Maria Fâni
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
• Texto Completo: https://patua.iec.gov.br/handle/iec/4560
• topic: Alcaloides / farmacologia; Extratos Vegetais / química; Leishmania / efeitos dos fármacos

This study evaluated the morphological changes caused by fractions and subfractions, obtained from barks of Aspidosperna nitidum, against L. (L.) amazonensis promastigotes. The ethanolic extract (EE) obtained through the maceration of trunk barks was subjected to an acid–base partition, resulting the neutral (FN) and the alkaloid (FA) fractions, and fractionation under refux, yielded hexane (FrHEX), dichloromethane (FrDCL), ethyl acetate (FrACoET), and methanol (FrMEOH) fractions. The FA was fractionated and three subfractions (SF5-6, SF8, and SF9) were obtained and analyzed by HPLC–DAD and 1 H NMR. The antipromastigote activity of all samples was evaluated by MTT, after that, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) for the active fractions were performed. Chromatographic analyzes suggest the presence of alkaloids in EE, FN, FA, and FrDCL. The fractionation of FA led to the isolation of the indole alkaloid dihydrocorynantheol (SF8 fractions). The SF5-6, dihydrocorynantheol and SF-9 samples were active against promastigotes, while FrDCL was moderately active. The SEM analysis revealed cell rounding and changes in the fagellum of the parasites. In the TEM analysis, the treated promastigotes showed changes in fagellar pocket and kinetoplast, and presence of lipid inclusions. These results suggest that alkaloids isolated from A. nitidum are promising as leishmanicidal.