Oxidative damage in human periodontal ligament fibroblast (hPLF) after methylmercury exposure
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/3996 |
Resumo: | Human exposure to mercury (Hg) is primary associated with its organic form, methylmercury (MeHg), through the ingestion of contaminated seafood. However, Hg contamination is also positively correlated with the number of dental restorations, total surface of amalgam, and organic mercury concentration in the saliva. Among the cells existing in the oral cavity, human periodontal ligament fibroblast (hPLF) cells are important cells responsible for the production of matrix and extracellular collagen, besides sustentation, renewal, repair, and tissue regeneration. In this way, the present study is aimed at investigating the potential oxidative effects caused by MeHg on hPLF. Firstly, we analyzed the cytotoxic effects of MeHg (general metabolism status, cell viability, and mercury accumulation) followed by the parameters related to oxidative stress (total antioxidant capacity, GSH levels, and DNA damage). Our results demonstrated that MeHg toxicity increased in accordance with the rise of MeHg concentration in the exposure solutions (1-7 μM) causing 100% of cell death at 7 μM MeHg exposure. The general metabolism status was firstly affected by 2 μM MeHg exposure (43:8±1:7%), while a significant decrease of cell viability has arisen significantly only at 3 μM MeHg exposure (68:7±1:4%). The ratio among these two analyses (named fold change) demonstrated viable hPLF with compromised cellular machinery along with the range of MeHg exposure. Subsequently, two distinct MeHg concentrations (0.3 and 3 μM) were chosen based on LC50 value (4.2 μM). hPLF exposed to these two MeHg concentrations showed an intracellular Hg accumulation as a linear-type saturation curve indicating that metal accumulated diffusively in the cells, typical for metal organic forms such as methyl. The levels of total GSH decreased 50% at exposure to 3 μM MeHg when compared to control. Finally, no alteration in the DNA integrity was observed at 0.3 μM MeHg exposure, but 3 μM MeHg caused significant damage. In conclusion, it was observed that MeHg exposure affected the general metabolism status of hPLF with no necessary decrease on the cell death. Additionally, although the oxidative imbalance in the hPLF was confirmed only at 3 μM MeHg through the increase of total GSH level and DNA damage, the lower concentration of MeHg used (0.3 μM) requires attention since the intracellular mercury accumulation may be toxic at chronic exposures. |
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Nogueira, Lygia SegaVasconcelos, Carolina PMitre, Geovanni PereiraKataoka, Maria Sueli da SilvaLima, Marcelo de OliveiraOliveira, Edivaldo Herculano Corrêa deLima, Rafael R2019-12-11T18:17:43Z2019-12-11T18:17:43Z2019NOGUEIRA, Lygia Sega et al. Oxidative damage in human periodontal ligament fibroblast (hPLF) after methylmercury exposure. Oxidative Medicine and Cellular Longevity, v. 2019, n. ID8470857, p. 1-7, 2019.1942-0994https://patua.iec.gov.br/handle/iec/399610.1155/2019/8470857Human exposure to mercury (Hg) is primary associated with its organic form, methylmercury (MeHg), through the ingestion of contaminated seafood. However, Hg contamination is also positively correlated with the number of dental restorations, total surface of amalgam, and organic mercury concentration in the saliva. Among the cells existing in the oral cavity, human periodontal ligament fibroblast (hPLF) cells are important cells responsible for the production of matrix and extracellular collagen, besides sustentation, renewal, repair, and tissue regeneration. In this way, the present study is aimed at investigating the potential oxidative effects caused by MeHg on hPLF. Firstly, we analyzed the cytotoxic effects of MeHg (general metabolism status, cell viability, and mercury accumulation) followed by the parameters related to oxidative stress (total antioxidant capacity, GSH levels, and DNA damage). Our results demonstrated that MeHg toxicity increased in accordance with the rise of MeHg concentration in the exposure solutions (1-7 μM) causing 100% of cell death at 7 μM MeHg exposure. The general metabolism status was firstly affected by 2 μM MeHg exposure (43:8±1:7%), while a significant decrease of cell viability has arisen significantly only at 3 μM MeHg exposure (68:7±1:4%). The ratio among these two analyses (named fold change) demonstrated viable hPLF with compromised cellular machinery along with the range of MeHg exposure. Subsequently, two distinct MeHg concentrations (0.3 and 3 μM) were chosen based on LC50 value (4.2 μM). hPLF exposed to these two MeHg concentrations showed an intracellular Hg accumulation as a linear-type saturation curve indicating that metal accumulated diffusively in the cells, typical for metal organic forms such as methyl. The levels of total GSH decreased 50% at exposure to 3 μM MeHg when compared to control. Finally, no alteration in the DNA integrity was observed at 0.3 μM MeHg exposure, but 3 μM MeHg caused significant damage. In conclusion, it was observed that MeHg exposure affected the general metabolism status of hPLF with no necessary decrease on the cell death. Additionally, although the oxidative imbalance in the hPLF was confirmed only at 3 μM MeHg through the increase of total GSH level and DNA damage, the lower concentration of MeHg used (0.3 μM) requires attention since the intracellular mercury accumulation may be toxic at chronic exposures.This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior-Brasil (CAPES)-Finance Code 001. Lygia S. Nogueira was supported by Programa Nacional de Pós-Graduação (PNPD/CAPES).Universidade Federal do Pará. Laboratório de Biologia Estrutural e Funcional. Belém, PA, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Citogenética e Cultura de Tecidos. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Citogenética e Cultura de Tecidos. Ananindeua, PA, Brasil.Universidade Federal do Pará. Laboratório de Cultura Celular. Belém, PA, Brazil.Universidade Federal do Pará. Laboratório de Cultura Celular. Belém, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Toxicologia. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Citogenética e Cultura de Tecidos. Ananindeua, PA, Brasil / Universidade Federal do Pará. Instituto de Ciências Exatas e Naturais. Belém, PA, Brazil.Universidade Federal do Pará. Laboratório de Biologia Estrutural e Funcional. Belém, PA, Brazil.engHindawi Publishing CorporationOxidative damage in human periodontal ligament fibroblast (hPLF) after methylmercury exposureinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleMercúrio / toxicidadeIntoxicação por MercúrioRestaurações IntracoronáriasLigamento Periodontal / citologiainfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALOxidative damage in human periodontal ligament fibroblast (hPLF) after methylmercury exposure.pdfOxidative damage in human periodontal ligament fibroblast (hPLF) after methylmercury exposure.pdfapplication/pdf1933539https://patua.iec.gov.br/bitstreams/c8c3f9a6-39e3-4b96-a16e-960104e83558/download03b29c5bdf38a06760c5b915143fb3adMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82182https://patua.iec.gov.br/bitstreams/81a39256-7588-4902-826d-6e97b2def592/download11832eea31b16df8613079d742d61793MD52TEXTOxidative damage in human periodontal ligament fibroblast (hPLF) after methylmercury exposure.pdf.txtOxidative damage in human periodontal ligament fibroblast (hPLF) after methylmercury exposure.pdf.txtExtracted texttext/plain37633https://patua.iec.gov.br/bitstreams/07eec6f6-23b0-4f24-af2e-dde4277b318a/download443b38047395491e6146a78a6490089fMD55THUMBNAILOxidative damage in human periodontal ligament fibroblast (hPLF) after methylmercury exposure.pdf.jpgOxidative damage in human periodontal ligament fibroblast (hPLF) after methylmercury exposure.pdf.jpgGenerated Thumbnailimage/jpeg5688https://patua.iec.gov.br/bitstreams/66a5d77b-01d4-453c-b94a-a4496bcc8701/download3cf309cbe764b6d55a0309450cb7dda4MD56iec/39962022-10-21 00:05:36.819oai:patua.iec.gov.br:iec/3996https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-21T00:05:36Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)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 |
dc.title.pt_BR.fl_str_mv |
Oxidative damage in human periodontal ligament fibroblast (hPLF) after methylmercury exposure |
title |
Oxidative damage in human periodontal ligament fibroblast (hPLF) after methylmercury exposure |
spellingShingle |
Oxidative damage in human periodontal ligament fibroblast (hPLF) after methylmercury exposure Nogueira, Lygia Sega Mercúrio / toxicidade Intoxicação por Mercúrio Restaurações Intracoronárias Ligamento Periodontal / citologia |
title_short |
Oxidative damage in human periodontal ligament fibroblast (hPLF) after methylmercury exposure |
title_full |
Oxidative damage in human periodontal ligament fibroblast (hPLF) after methylmercury exposure |
title_fullStr |
Oxidative damage in human periodontal ligament fibroblast (hPLF) after methylmercury exposure |
title_full_unstemmed |
Oxidative damage in human periodontal ligament fibroblast (hPLF) after methylmercury exposure |
title_sort |
Oxidative damage in human periodontal ligament fibroblast (hPLF) after methylmercury exposure |
author |
Nogueira, Lygia Sega |
author_facet |
Nogueira, Lygia Sega Vasconcelos, Carolina P Mitre, Geovanni Pereira Kataoka, Maria Sueli da Silva Lima, Marcelo de Oliveira Oliveira, Edivaldo Herculano Corrêa de Lima, Rafael R |
author_role |
author |
author2 |
Vasconcelos, Carolina P Mitre, Geovanni Pereira Kataoka, Maria Sueli da Silva Lima, Marcelo de Oliveira Oliveira, Edivaldo Herculano Corrêa de Lima, Rafael R |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Nogueira, Lygia Sega Vasconcelos, Carolina P Mitre, Geovanni Pereira Kataoka, Maria Sueli da Silva Lima, Marcelo de Oliveira Oliveira, Edivaldo Herculano Corrêa de Lima, Rafael R |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Mercúrio / toxicidade Intoxicação por Mercúrio Restaurações Intracoronárias Ligamento Periodontal / citologia |
topic |
Mercúrio / toxicidade Intoxicação por Mercúrio Restaurações Intracoronárias Ligamento Periodontal / citologia |
description |
Human exposure to mercury (Hg) is primary associated with its organic form, methylmercury (MeHg), through the ingestion of contaminated seafood. However, Hg contamination is also positively correlated with the number of dental restorations, total surface of amalgam, and organic mercury concentration in the saliva. Among the cells existing in the oral cavity, human periodontal ligament fibroblast (hPLF) cells are important cells responsible for the production of matrix and extracellular collagen, besides sustentation, renewal, repair, and tissue regeneration. In this way, the present study is aimed at investigating the potential oxidative effects caused by MeHg on hPLF. Firstly, we analyzed the cytotoxic effects of MeHg (general metabolism status, cell viability, and mercury accumulation) followed by the parameters related to oxidative stress (total antioxidant capacity, GSH levels, and DNA damage). Our results demonstrated that MeHg toxicity increased in accordance with the rise of MeHg concentration in the exposure solutions (1-7 μM) causing 100% of cell death at 7 μM MeHg exposure. The general metabolism status was firstly affected by 2 μM MeHg exposure (43:8±1:7%), while a significant decrease of cell viability has arisen significantly only at 3 μM MeHg exposure (68:7±1:4%). The ratio among these two analyses (named fold change) demonstrated viable hPLF with compromised cellular machinery along with the range of MeHg exposure. Subsequently, two distinct MeHg concentrations (0.3 and 3 μM) were chosen based on LC50 value (4.2 μM). hPLF exposed to these two MeHg concentrations showed an intracellular Hg accumulation as a linear-type saturation curve indicating that metal accumulated diffusively in the cells, typical for metal organic forms such as methyl. The levels of total GSH decreased 50% at exposure to 3 μM MeHg when compared to control. Finally, no alteration in the DNA integrity was observed at 0.3 μM MeHg exposure, but 3 μM MeHg caused significant damage. In conclusion, it was observed that MeHg exposure affected the general metabolism status of hPLF with no necessary decrease on the cell death. Additionally, although the oxidative imbalance in the hPLF was confirmed only at 3 μM MeHg through the increase of total GSH level and DNA damage, the lower concentration of MeHg used (0.3 μM) requires attention since the intracellular mercury accumulation may be toxic at chronic exposures. |
publishDate |
2019 |
dc.date.accessioned.fl_str_mv |
2019-12-11T18:17:43Z |
dc.date.available.fl_str_mv |
2019-12-11T18:17:43Z |
dc.date.issued.fl_str_mv |
2019 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
NOGUEIRA, Lygia Sega et al. Oxidative damage in human periodontal ligament fibroblast (hPLF) after methylmercury exposure. Oxidative Medicine and Cellular Longevity, v. 2019, n. ID8470857, p. 1-7, 2019. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/3996 |
dc.identifier.issn.-.fl_str_mv |
1942-0994 |
dc.identifier.doi.-.fl_str_mv |
10.1155/2019/8470857 |
identifier_str_mv |
NOGUEIRA, Lygia Sega et al. Oxidative damage in human periodontal ligament fibroblast (hPLF) after methylmercury exposure. Oxidative Medicine and Cellular Longevity, v. 2019, n. ID8470857, p. 1-7, 2019. 1942-0994 10.1155/2019/8470857 |
url |
https://patua.iec.gov.br/handle/iec/3996 |
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eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
Hindawi Publishing Corporation |
publisher.none.fl_str_mv |
Hindawi Publishing Corporation |
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reponame:Repositório Digital do Instituto Evandro Chagas (Patuá) instname:Instituto Evandro Chagas (IEC) instacron:IEC |
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IEC |
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Repositório Digital do Instituto Evandro Chagas (Patuá) |
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