Experimental cutaneous leishmaniasis IV : the humoral response of Cebus paella (Primates: Cebidae) to infections of Leishmania (Leishmania) amazonensis, L. (Viannia) lainsoni and L. (V.) braziliensis using the direct agglutination test

• Autor(a) principal: Santos, Lourdes Maria Garcez dos
• Data de Publicação: 1997
• Outros Autores: Silveira, Fernando Tobias, El Harith, Abdallah, Lainson, Ralph, Shaw, Jeffrey Jon
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
• Texto Completo: https://patua.iec.gov.br/handle/iec/3748
• topic: Primatas / anatomia & histologia; Leishmania braziliensis / parasitologia; Leishmaniose Cutânea / diagnóstico; Cebus / parasitologia; Testes de Aglutinação - métodos; Formação de Anticorpos

The direct agglutination test (DAT) was used to evaluate the serological response of 150 serum samples taken from 15 captive-bred capuchin monkeys Cebus apella. These animals had been experimentally infected with either L. (Leishmania ) amazonensis, L. (Viannia) lainsoni or L. (V.) braziliensis. Monkeys infected with L. (L.) amazonensis or L. (V.) lainsoni were challenged with the homologous parasite one month after their spontaneous cure. DAT antigens were prepared from L. (L.) dono6ani, L. (L.) amazonensis and L. (V.) braziliensis. Antigens were difficult to standardise and it was impossible to produce an L. (V.) lainsoni antigen as parasites remained aggregated even after trypsinization. The DAT detected significant humoral responses in all the infected monkeys. Titres were higher when homologous antigens were used, especially in secondary responses. This suggests that homologous antigen should be used to detect antibodies in human cutaneous leishmaniasis.