Characterization of Mycobacterium tuberculosis var. africanum isolated from a patient with pulmonary tuberculosis in Brazil

Detalhes bibliográficos
Autor(a) principal: Rabahi, Marcelo Fouad
Data de Publicação: 2020
Outros Autores: Conceição, Emilyn Costa, Paiva, Luísa Oliveira de, Souto, Marcos Vinícius Muniz Lemes, Sisco, Maria Carolina, Waard, Jacobus de, Caldas, Paulo Cesar de Souza, Fandinho, Fatima, Ramos, Jesus Pais, Carvalho, Luciana Distasio de, Campos, Carlos Eduardo Dias, Lima, Karla Valéria Batista, Silva, Sandro Patroca da, Sharma, Abhinav, Robledo, Jaime, Paez, Uriel Alonso Hurtado, Duarte, Rafael Silva, Silva, Marlei Gomes da, Gomes, Lia Lima, Vasconcellos, Sidra Ezidio Gonçalves, Uwezeye, Cecile, Jong, Bouke C. de, Junqueira-Kipnis, Ana Paula, Suffys, Philip Noel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
Texto Completo: http://patua.iec.gov.br//handle/iec/4181
Resumo: Human tuberculosis (TB) is caused by members of the Mycobacterium tuberculosis complex (MTBC), including Mycobacterium tuberculosis var. tuberculosis (MTB) and Mycobacterium tuberculosis var. africanum (MAF). While MTB is isolated worldwide, MAF is almost completely restricted to the African continent, and despite the historical proximity between Brazil and Africa during the slave trade, no case of TB being caused by MAF has been reported in Brazil to date. We hereby describe the first case of TB caused by MAF in Brazil comparing its genome against the published ones. A female patient who had never visited Africa presented with clinical symptoms typical of pulmonary TB. Based on 16S rRNA gene sequencing, the cultured isolate was identified as belonging to MTBC and partial sequence of the hsp65 gene was identical to that of MAF. This was confirmed by genotyping based on detection of Single Nucleotide Polymorphism (SNP), Region of Difference (RD) and spoligotyping. The isolate presented the Shared International Typing (SIT) 181. In the whole-genome comparison against MAF genomes available on published EMBL-EBI European Nucleotide Archive (ENA), the Brazilian genome (MAFBRA00707) was identified as belonging to Lineage 6 and clustered with isolates from The Gambia. This is the first report of the isolation of MAF from a patient from Brazil, without evidence of having any contact with an African index case
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spelling Rabahi, Marcelo FouadConceição, Emilyn CostaPaiva, Luísa Oliveira deSouto, Marcos Vinícius Muniz LemesSisco, Maria CarolinaWaard, Jacobus deCaldas, Paulo Cesar de SouzaFandinho, FatimaRamos, Jesus PaisCarvalho, Luciana Distasio deCampos, Carlos Eduardo DiasLima, Karla Valéria BatistaSilva, Sandro Patroca daSharma, AbhinavRobledo, JaimePaez, Uriel Alonso HurtadoDuarte, Rafael SilvaSilva, Marlei Gomes daGomes, Lia LimaVasconcellos, Sidra Ezidio GonçalvesUwezeye, CecileJong, Bouke C. deJunqueira-Kipnis, Ana PaulaSuffys, Philip Noel2020-10-13T16:48:44Z2020-10-13T16:48:44Z2020RABAHI, Marcelo Fouad et al. Characterization of Mycobacterium tuberculosis var. africanum isolated from a patient with pulmonary tuberculosis in Brazil. Infection, Genetics and Evolution, v. 85, n. 104520, p.xx, Nov. 2020.1567-1348http://patua.iec.gov.br//handle/iec/418110.1016/j.meegid.2020.104550Human tuberculosis (TB) is caused by members of the Mycobacterium tuberculosis complex (MTBC), including Mycobacterium tuberculosis var. tuberculosis (MTB) and Mycobacterium tuberculosis var. africanum (MAF). While MTB is isolated worldwide, MAF is almost completely restricted to the African continent, and despite the historical proximity between Brazil and Africa during the slave trade, no case of TB being caused by MAF has been reported in Brazil to date. We hereby describe the first case of TB caused by MAF in Brazil comparing its genome against the published ones. A female patient who had never visited Africa presented with clinical symptoms typical of pulmonary TB. Based on 16S rRNA gene sequencing, the cultured isolate was identified as belonging to MTBC and partial sequence of the hsp65 gene was identical to that of MAF. This was confirmed by genotyping based on detection of Single Nucleotide Polymorphism (SNP), Region of Difference (RD) and spoligotyping. The isolate presented the Shared International Typing (SIT) 181. In the whole-genome comparison against MAF genomes available on published EMBL-EBI European Nucleotide Archive (ENA), the Brazilian genome (MAFBRA00707) was identified as belonging to Lineage 6 and clustered with isolates from The Gambia. This is the first report of the isolation of MAF from a patient from Brazil, without evidence of having any contact with an African index caseUniversidade Federal de Goiás. Hospital das Clínicas. Goiânia, GO, Brazil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Programa de Pós-graduação em Pesquisa Clínica e Doenças Infecciosas. Rio de Janeiro, RJ, Brazil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada em Micobacterias. Rio de Janeiro, RJ, Brazil.Universidade Federal de Goiás. Hospital das Clínicas. Goiânia, GO, Brazil.Universidade Federal de Goiás. Hospital das Clínicas. Goiânia, GO, Brazil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada em Micobacterias. Rio de Janeiro, RJ, Brazil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Rio de Janeiro, RJ, Brazil.Instituto de Biomedicina Dr. Jacinto Convit. Instituto de Biomedicina. San Jose, Caracas, Venezuela / Universidad de Las Américas. Facultad de Ciencias de La Salud. One Health Research Group. Quito, Ecuador.Fundação Oswaldo Cruz. Centro de Referência Professor Hélio Fraga. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Centro de Referência Professor Hélio Fraga. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Centro de Referência Professor Hélio Fraga. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Centro de Referência Professor Hélio Fraga. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Centro de Referência Professor Hélio Fraga. Rio de Janeiro, RJ, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.International Institute of InformationTechnology - Bangalore. Department of Data Science. Bangalore, India.Corporación para Investigaciones Biológicas. Medellín, Colombia.Corporación para Investigaciones Biológicas. Medellín, Colombia.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Rio de Janeiro, RJ, Brazil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada em Micobacterias. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada em Micobacterias. Rio de Janeiro, RJ, Brazil.Institute of Tropical Medicine. Mycobacteriology Unit. Antwerp, Belgium.Institute of Tropical Medicine. Mycobacteriology Unit. Antwerp, Belgium.Universidade Federal de Goiás. Instituto de Patologia Tropical e Saúde Pública. Goiânia, GO, Brazil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada em Micobacterias. Rio de Janeiro, RJ, Brazil.engElsevierCharacterization of Mycobacterium tuberculosis var. africanum isolated from a patient with pulmonary tuberculosis in Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleTuberculose Pulmonar / patologiaMycobacterium tuberculosis / patogenicidadeMycobacterium tuberculosis var. africanumGenoma BacterianoRelatos de Casosinfo:eu-repo/semantics/embargoedAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALCharacterization of Mycobacterium tuberculosis var. africanum isolated from a patient with pulmonary tuberculosis in Brazil.pdfCharacterization of Mycobacterium tuberculosis var. africanum isolated from a patient with pulmonary tuberculosis in Brazil.pdfapplication/pdf551083https://patua.iec.gov.br/bitstreams/f4453fe7-2b10-4247-902b-a9bad4a17445/downloadc9a9c128e29cac82a5d7fdf3f4e6da73MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82182https://patua.iec.gov.br/bitstreams/c13e68ef-349d-446b-a91d-b773b2c1aa07/download11832eea31b16df8613079d742d61793MD52TEXTCharacterization of Mycobacterium tuberculosis var. africanum isolated from a patient with pulmonary tuberculosis in Brazil.pdf.txtCharacterization of Mycobacterium tuberculosis var. africanum isolated from a patient with pulmonary tuberculosis in Brazil.pdf.txtExtracted texttext/plain2https://patua.iec.gov.br/bitstreams/7f2208f2-70c6-4d8a-804c-1a7e49e5fc4f/downloade1c06d85ae7b8b032bef47e42e4c08f9MD55THUMBNAILCharacterization of Mycobacterium tuberculosis var. africanum isolated from a patient with pulmonary tuberculosis in Brazil.pdf.jpgCharacterization of Mycobacterium tuberculosis var. africanum isolated from a patient with pulmonary tuberculosis in Brazil.pdf.jpgGenerated Thumbnailimage/jpeg3095https://patua.iec.gov.br/bitstreams/b0864243-1cc6-456a-8af7-cf2ec6c015b7/download71859d578212107f7f8c49a4ce09d9eeMD56iec/41812022-10-20 21:08:44.989oai:patua.iec.gov.br:iec/4181https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-20T21:08:44Repositório Digital do Instituto Evandro Chagas (Patuá) - 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dc.title.pt_BR.fl_str_mv Characterization of Mycobacterium tuberculosis var. africanum isolated from a patient with pulmonary tuberculosis in Brazil
title Characterization of Mycobacterium tuberculosis var. africanum isolated from a patient with pulmonary tuberculosis in Brazil
spellingShingle Characterization of Mycobacterium tuberculosis var. africanum isolated from a patient with pulmonary tuberculosis in Brazil
Rabahi, Marcelo Fouad
Tuberculose Pulmonar / patologia
Mycobacterium tuberculosis / patogenicidade
Mycobacterium tuberculosis var. africanum
Genoma Bacteriano
Relatos de Casos
title_short Characterization of Mycobacterium tuberculosis var. africanum isolated from a patient with pulmonary tuberculosis in Brazil
title_full Characterization of Mycobacterium tuberculosis var. africanum isolated from a patient with pulmonary tuberculosis in Brazil
title_fullStr Characterization of Mycobacterium tuberculosis var. africanum isolated from a patient with pulmonary tuberculosis in Brazil
title_full_unstemmed Characterization of Mycobacterium tuberculosis var. africanum isolated from a patient with pulmonary tuberculosis in Brazil
title_sort Characterization of Mycobacterium tuberculosis var. africanum isolated from a patient with pulmonary tuberculosis in Brazil
author Rabahi, Marcelo Fouad
author_facet Rabahi, Marcelo Fouad
Conceição, Emilyn Costa
Paiva, Luísa Oliveira de
Souto, Marcos Vinícius Muniz Lemes
Sisco, Maria Carolina
Waard, Jacobus de
Caldas, Paulo Cesar de Souza
Fandinho, Fatima
Ramos, Jesus Pais
Carvalho, Luciana Distasio de
Campos, Carlos Eduardo Dias
Lima, Karla Valéria Batista
Silva, Sandro Patroca da
Sharma, Abhinav
Robledo, Jaime
Paez, Uriel Alonso Hurtado
Duarte, Rafael Silva
Silva, Marlei Gomes da
Gomes, Lia Lima
Vasconcellos, Sidra Ezidio Gonçalves
Uwezeye, Cecile
Jong, Bouke C. de
Junqueira-Kipnis, Ana Paula
Suffys, Philip Noel
author_role author
author2 Conceição, Emilyn Costa
Paiva, Luísa Oliveira de
Souto, Marcos Vinícius Muniz Lemes
Sisco, Maria Carolina
Waard, Jacobus de
Caldas, Paulo Cesar de Souza
Fandinho, Fatima
Ramos, Jesus Pais
Carvalho, Luciana Distasio de
Campos, Carlos Eduardo Dias
Lima, Karla Valéria Batista
Silva, Sandro Patroca da
Sharma, Abhinav
Robledo, Jaime
Paez, Uriel Alonso Hurtado
Duarte, Rafael Silva
Silva, Marlei Gomes da
Gomes, Lia Lima
Vasconcellos, Sidra Ezidio Gonçalves
Uwezeye, Cecile
Jong, Bouke C. de
Junqueira-Kipnis, Ana Paula
Suffys, Philip Noel
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Rabahi, Marcelo Fouad
Conceição, Emilyn Costa
Paiva, Luísa Oliveira de
Souto, Marcos Vinícius Muniz Lemes
Sisco, Maria Carolina
Waard, Jacobus de
Caldas, Paulo Cesar de Souza
Fandinho, Fatima
Ramos, Jesus Pais
Carvalho, Luciana Distasio de
Campos, Carlos Eduardo Dias
Lima, Karla Valéria Batista
Silva, Sandro Patroca da
Sharma, Abhinav
Robledo, Jaime
Paez, Uriel Alonso Hurtado
Duarte, Rafael Silva
Silva, Marlei Gomes da
Gomes, Lia Lima
Vasconcellos, Sidra Ezidio Gonçalves
Uwezeye, Cecile
Jong, Bouke C. de
Junqueira-Kipnis, Ana Paula
Suffys, Philip Noel
dc.subject.decsPrimary.pt_BR.fl_str_mv Tuberculose Pulmonar / patologia
Mycobacterium tuberculosis / patogenicidade
Mycobacterium tuberculosis var. africanum
Genoma Bacteriano
Relatos de Casos
topic Tuberculose Pulmonar / patologia
Mycobacterium tuberculosis / patogenicidade
Mycobacterium tuberculosis var. africanum
Genoma Bacteriano
Relatos de Casos
description Human tuberculosis (TB) is caused by members of the Mycobacterium tuberculosis complex (MTBC), including Mycobacterium tuberculosis var. tuberculosis (MTB) and Mycobacterium tuberculosis var. africanum (MAF). While MTB is isolated worldwide, MAF is almost completely restricted to the African continent, and despite the historical proximity between Brazil and Africa during the slave trade, no case of TB being caused by MAF has been reported in Brazil to date. We hereby describe the first case of TB caused by MAF in Brazil comparing its genome against the published ones. A female patient who had never visited Africa presented with clinical symptoms typical of pulmonary TB. Based on 16S rRNA gene sequencing, the cultured isolate was identified as belonging to MTBC and partial sequence of the hsp65 gene was identical to that of MAF. This was confirmed by genotyping based on detection of Single Nucleotide Polymorphism (SNP), Region of Difference (RD) and spoligotyping. The isolate presented the Shared International Typing (SIT) 181. In the whole-genome comparison against MAF genomes available on published EMBL-EBI European Nucleotide Archive (ENA), the Brazilian genome (MAFBRA00707) was identified as belonging to Lineage 6 and clustered with isolates from The Gambia. This is the first report of the isolation of MAF from a patient from Brazil, without evidence of having any contact with an African index case
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-10-13T16:48:44Z
dc.date.available.fl_str_mv 2020-10-13T16:48:44Z
dc.date.issued.fl_str_mv 2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.citation.fl_str_mv RABAHI, Marcelo Fouad et al. Characterization of Mycobacterium tuberculosis var. africanum isolated from a patient with pulmonary tuberculosis in Brazil. Infection, Genetics and Evolution, v. 85, n. 104520, p.xx, Nov. 2020.
dc.identifier.uri.fl_str_mv http://patua.iec.gov.br//handle/iec/4181
dc.identifier.issn.-.fl_str_mv 1567-1348
dc.identifier.doi.-.fl_str_mv 10.1016/j.meegid.2020.104550
identifier_str_mv RABAHI, Marcelo Fouad et al. Characterization of Mycobacterium tuberculosis var. africanum isolated from a patient with pulmonary tuberculosis in Brazil. Infection, Genetics and Evolution, v. 85, n. 104520, p.xx, Nov. 2020.
1567-1348
10.1016/j.meegid.2020.104550
url http://patua.iec.gov.br//handle/iec/4181
dc.language.iso.fl_str_mv eng
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dc.publisher.none.fl_str_mv Elsevier
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