Limbic encephalitis brain damage induced by Cocal Virus in adult mice is reduced by environmental enrichment: neuropathological and behavioral studies
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/4250 |
Resumo: | We previously demonstrated, using the Piry virus model, that environmental enrichment promotes higher T-cell infiltration, fewer microglial changes, and faster central nervous system (CNS) virus clearance in adult mice. However, little is known about disease progression, behavioral changes, CNS cytokine concentration, and neuropathology in limbic encephalitis in experimental models. Using Cocal virus, we infected C57Bl6 adult mice and studied the neuroanatomical distribution of viral antigens in correlation with the microglial morphological response, measured the CNS cytokine concentration, and assessed behavioral changes. C57Bl6 adult mice were maintained in an impoverished environment (IE) or enriched environment (EE) for four months and then subjected to the open field test. Afterwards, an equal volume of normal or virus-infected brain homogenate was nasally instilled. The brains were processed to detect viral antigens and microglial morphological changes using selective immunolabeling. We demonstrated earlier significant weight loss and higher mortality in IE mice. Additionally, behavioral analysis revealed a significant influence of the environment on locomotor and exploratory activity that was associated with less neuroinvasion and a reduced microglial response. Thus, environmental enrichment was associated with a more effective immune response in a mouse model of limbic encephalitis, allowing faster viral clearance/decreased viral dissemination, reduced disease progression, and less CNS damage. |
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Freitas, Priscilla dos Santos LieuthierLima, Ana Victória de LimaCarvalho, Karina Glazianne BarbosaCabral, Tatyane da SilvaFarias, Alexandre Maia deRodrigues, Ana Paula DrummondDiniz, Daniel GuerreiroDinz, Cristovam Wanderley PicançoDiniz Junior, José Antônio Picanço2021-02-08T17:38:48Z2021-02-08T17:38:48Z2021FREITAS, Priscilla dos Santos Lieuthier et al. Limbic encephalitis brain damage induced by Cocal Virus in adult mice is reduced by environmental enrichment: neuropathological and behavioral studies. Viruses, v. 13, n. 1, p. 1-21, 2021.1999-4915https://patua.iec.gov.br/handle/iec/425010.3390/v13010048We previously demonstrated, using the Piry virus model, that environmental enrichment promotes higher T-cell infiltration, fewer microglial changes, and faster central nervous system (CNS) virus clearance in adult mice. However, little is known about disease progression, behavioral changes, CNS cytokine concentration, and neuropathology in limbic encephalitis in experimental models. Using Cocal virus, we infected C57Bl6 adult mice and studied the neuroanatomical distribution of viral antigens in correlation with the microglial morphological response, measured the CNS cytokine concentration, and assessed behavioral changes. C57Bl6 adult mice were maintained in an impoverished environment (IE) or enriched environment (EE) for four months and then subjected to the open field test. Afterwards, an equal volume of normal or virus-infected brain homogenate was nasally instilled. The brains were processed to detect viral antigens and microglial morphological changes using selective immunolabeling. We demonstrated earlier significant weight loss and higher mortality in IE mice. Additionally, behavioral analysis revealed a significant influence of the environment on locomotor and exploratory activity that was associated with less neuroinvasion and a reduced microglial response. Thus, environmental enrichment was associated with a more effective immune response in a mouse model of limbic encephalitis, allowing faster viral clearance/decreased viral dissemination, reduced disease progression, and less CNS damage.This research was funded by Instituto Evandro Chagas/Ministério da Saúde, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), grant number 1466298.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Microscopia Eletrônica. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Microscopia Eletrônica. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Microscopia Eletrônica. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Microscopia Eletrônica. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Microscopia Eletrônica. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Microscopia Eletrônica. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Microscopia Eletrônica. Ananindeua, PA, Brasil / Universidade Federal do Pará. Instituto de Ciências Biológicas. Hospital Universitário João de Barros Barreto. Laboratório de Investigações em Neurodegeneração e Infecção. Belém, PA, Brazil.Universidade Federal do Pará. Instituto de Ciências Biológicas. Hospital Universitário João de Barros Barreto. Laboratório de Investigações em Neurodegeneração e Infecção. Belém, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Microscopia Eletrônica. 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dc.title.pt_BR.fl_str_mv |
Limbic encephalitis brain damage induced by Cocal Virus in adult mice is reduced by environmental enrichment: neuropathological and behavioral studies |
title |
Limbic encephalitis brain damage induced by Cocal Virus in adult mice is reduced by environmental enrichment: neuropathological and behavioral studies |
spellingShingle |
Limbic encephalitis brain damage induced by Cocal Virus in adult mice is reduced by environmental enrichment: neuropathological and behavioral studies Freitas, Priscilla dos Santos Lieuthier Camundongos / anatomia & histologia Sistema Nervoso Central / patologia Encefalite Límbica Vírus Cocal Ambiente Controlado Relação Dose-Resposta Imunológica |
title_short |
Limbic encephalitis brain damage induced by Cocal Virus in adult mice is reduced by environmental enrichment: neuropathological and behavioral studies |
title_full |
Limbic encephalitis brain damage induced by Cocal Virus in adult mice is reduced by environmental enrichment: neuropathological and behavioral studies |
title_fullStr |
Limbic encephalitis brain damage induced by Cocal Virus in adult mice is reduced by environmental enrichment: neuropathological and behavioral studies |
title_full_unstemmed |
Limbic encephalitis brain damage induced by Cocal Virus in adult mice is reduced by environmental enrichment: neuropathological and behavioral studies |
title_sort |
Limbic encephalitis brain damage induced by Cocal Virus in adult mice is reduced by environmental enrichment: neuropathological and behavioral studies |
author |
Freitas, Priscilla dos Santos Lieuthier |
author_facet |
Freitas, Priscilla dos Santos Lieuthier Lima, Ana Victória de Lima Carvalho, Karina Glazianne Barbosa Cabral, Tatyane da Silva Farias, Alexandre Maia de Rodrigues, Ana Paula Drummond Diniz, Daniel Guerreiro Dinz, Cristovam Wanderley Picanço Diniz Junior, José Antônio Picanço |
author_role |
author |
author2 |
Lima, Ana Victória de Lima Carvalho, Karina Glazianne Barbosa Cabral, Tatyane da Silva Farias, Alexandre Maia de Rodrigues, Ana Paula Drummond Diniz, Daniel Guerreiro Dinz, Cristovam Wanderley Picanço Diniz Junior, José Antônio Picanço |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Freitas, Priscilla dos Santos Lieuthier Lima, Ana Victória de Lima Carvalho, Karina Glazianne Barbosa Cabral, Tatyane da Silva Farias, Alexandre Maia de Rodrigues, Ana Paula Drummond Diniz, Daniel Guerreiro Dinz, Cristovam Wanderley Picanço Diniz Junior, José Antônio Picanço |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Camundongos / anatomia & histologia Sistema Nervoso Central / patologia Encefalite Límbica Vírus Cocal Ambiente Controlado Relação Dose-Resposta Imunológica |
topic |
Camundongos / anatomia & histologia Sistema Nervoso Central / patologia Encefalite Límbica Vírus Cocal Ambiente Controlado Relação Dose-Resposta Imunológica |
description |
We previously demonstrated, using the Piry virus model, that environmental enrichment promotes higher T-cell infiltration, fewer microglial changes, and faster central nervous system (CNS) virus clearance in adult mice. However, little is known about disease progression, behavioral changes, CNS cytokine concentration, and neuropathology in limbic encephalitis in experimental models. Using Cocal virus, we infected C57Bl6 adult mice and studied the neuroanatomical distribution of viral antigens in correlation with the microglial morphological response, measured the CNS cytokine concentration, and assessed behavioral changes. C57Bl6 adult mice were maintained in an impoverished environment (IE) or enriched environment (EE) for four months and then subjected to the open field test. Afterwards, an equal volume of normal or virus-infected brain homogenate was nasally instilled. The brains were processed to detect viral antigens and microglial morphological changes using selective immunolabeling. We demonstrated earlier significant weight loss and higher mortality in IE mice. Additionally, behavioral analysis revealed a significant influence of the environment on locomotor and exploratory activity that was associated with less neuroinvasion and a reduced microglial response. Thus, environmental enrichment was associated with a more effective immune response in a mouse model of limbic encephalitis, allowing faster viral clearance/decreased viral dissemination, reduced disease progression, and less CNS damage. |
publishDate |
2021 |
dc.date.accessioned.fl_str_mv |
2021-02-08T17:38:48Z |
dc.date.available.fl_str_mv |
2021-02-08T17:38:48Z |
dc.date.issued.fl_str_mv |
2021 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
FREITAS, Priscilla dos Santos Lieuthier et al. Limbic encephalitis brain damage induced by Cocal Virus in adult mice is reduced by environmental enrichment: neuropathological and behavioral studies. Viruses, v. 13, n. 1, p. 1-21, 2021. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/4250 |
dc.identifier.issn.-.fl_str_mv |
1999-4915 |
dc.identifier.doi.-.fl_str_mv |
10.3390/v13010048 |
identifier_str_mv |
FREITAS, Priscilla dos Santos Lieuthier et al. Limbic encephalitis brain damage induced by Cocal Virus in adult mice is reduced by environmental enrichment: neuropathological and behavioral studies. Viruses, v. 13, n. 1, p. 1-21, 2021. 1999-4915 10.3390/v13010048 |
url |
https://patua.iec.gov.br/handle/iec/4250 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
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Instituto Evandro Chagas (IEC) |
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IEC |
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Repositório Digital do Instituto Evandro Chagas (Patuá) |
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Repositório Digital do Instituto Evandro Chagas (Patuá) |
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