Molecular characterization of Capim and Enseada orthobunyaviruses

• Autor(a) principal: Souza, William Marciel de
• Data de Publicação: 2016
• Outros Autores: Acrani, Gustavo Olszanski, Romeiro, Marilia Farignoli, Reis Júnior, Osvaldo, Tolardo, Aline Lavado, Silva, Sandro Patroca da, Medeiros, Daniele Barbosa de Almeida, Varela, Mariana, Nunes, Márcio Roberto Teixeira, Figueiredo, Luiz Tadeu Moraes
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
• Texto Completo: https://patua.iec.gov.br/handle/iec/2510
• topic: Orthobunyavirus; Mapeamento Cromossômico; Phlebovirus; Variação Genética; Capim orthobunyavirus; Enseada orthobunyavirus

Capim and Enseada viruses are members of the genus Orthobunyavirus isolated from mosquitoes and mammals in Brazil. Despite seroprevalence studies indicating human infections in Latin America, these viruses remain relatively unknown and unstudied. In order to better understand the genetic and evolutionary relationships among orthobunyaviruses, we sequenced the three genomic segments of Capim and Enseada orthobunyaviruses. Based on phylogenetic analysis, we demonstrated that these viruses depicted two new distinct clades, one represented by Enseada and another composed of Capim virus. In general, the genome organization and genetic traits of these viruses are similar to other orthobunyaviruses however, the open reading frame (ORF) of the putative nonstructural NSs protein of Enseada orthobunyavirus precedes the nucleocapsid ORF. Overall, our study provides details on the molecular characteristics of the prototype species of two groups within the Orthobunyavirus genus, revealing novel features into the genetic diversity and evolution of this genus.