Estradiol, but not dehydroepiandrosterone, decreases parasitemia and increases the incidence of cerebral malaria and the mortality in Plasmodium berghei ANKA-infected CBA mice

Detalhes bibliográficos
Autor(a) principal: Libonati, Rosana Maria Feio
Data de Publicação: 2006
Outros Autores: Cunha, Maristela G, Souza, José Maria de, Santos, Marcos V. N, Oliveira, Salma Gomes de, Daniel-Ribeiro, Cláudio Tadeu, Carvalho, Leonardo José de Moura, Nascimento, José L. M. do
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
Texto Completo: https://patua.iec.gov.br/handle/iec/1053
Resumo: Objective: The effect of castration and subsequent replacement of dehydroepiandrosterone (DHEA) or estradiol on parasitemia, mortality and incidence of cerebral malaria (CM) was evaluated in CBA mice infected with Plasmodium berghei ANKA. Methods: Female mice were castrated, and groups of 12–15 animals received daily injections of DHEA, estradiol or saline. Four days after the start of treatment, mice were inoculated with 1 ! 106 P. berghei ANKA-parasitized erythrocytes. DHEA treatment was continued during the 5 days after infection, and estradiol was administered during the follow-up. Parasitemia was evaluated daily in Giemsastained blood smears. Signs of CM were determined by the manifestation of coma, limb paralysis and/or convulsions. Plasma TNF- levels were evaluated by sandwich ELISA. Nitric oxide synthase (NOS) activity in the brain of moribund mice was measured by the method of Bredt and Snyder. Results: In non-castrated infected mice, the incidence of CM was 50%, and plasma TNF- increased and brain NOS activity decreased compared to non-infected controls. Castration had no major effect on the parameters analyzed (parasitemia, mortality, CM incidence, TNF- levels or NOS activity). Estradiol replacement caused a decrease in parasitemia but resulted in higher CM incidence and faster mortality, with an increase in NOS activity. Conclusions: Estradiol modulated the immune response of P. berghei ANKA-infected CBA mice, decreasing parasitemia and increasing NOS activity, and impacted negatively on survival and CM incidence, showing that neuroimmunoendocrine interactions are important in the physiopathogenesis of malaria infections.
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spelling Libonati, Rosana Maria FeioCunha, Maristela GSouza, José Maria deSantos, Marcos V. NOliveira, Salma Gomes deDaniel-Ribeiro, Cláudio TadeuCarvalho, Leonardo José de MouraNascimento, José L. M. do2016-01-26T11:43:20Z2016-01-26T11:43:20Z2006LIBONATI, Rosana Maria Feio et al. Estradiol, but not dehydroepiandrosterone, decreases parasitemia and increases the incidence of cerebral malaria and the mortality in Plasmodium berghei ANKA-infected CBA mice. NeuroImmunoModulation, v. 13, n. 1, p. 28-35, 20061021-7401https://patua.iec.gov.br/handle/iec/105310.1159/000093271Objective: The effect of castration and subsequent replacement of dehydroepiandrosterone (DHEA) or estradiol on parasitemia, mortality and incidence of cerebral malaria (CM) was evaluated in CBA mice infected with Plasmodium berghei ANKA. Methods: Female mice were castrated, and groups of 12–15 animals received daily injections of DHEA, estradiol or saline. Four days after the start of treatment, mice were inoculated with 1 ! 106 P. berghei ANKA-parasitized erythrocytes. DHEA treatment was continued during the 5 days after infection, and estradiol was administered during the follow-up. Parasitemia was evaluated daily in Giemsastained blood smears. Signs of CM were determined by the manifestation of coma, limb paralysis and/or convulsions. Plasma TNF- levels were evaluated by sandwich ELISA. Nitric oxide synthase (NOS) activity in the brain of moribund mice was measured by the method of Bredt and Snyder. Results: In non-castrated infected mice, the incidence of CM was 50%, and plasma TNF- increased and brain NOS activity decreased compared to non-infected controls. Castration had no major effect on the parameters analyzed (parasitemia, mortality, CM incidence, TNF- levels or NOS activity). Estradiol replacement caused a decrease in parasitemia but resulted in higher CM incidence and faster mortality, with an increase in NOS activity. Conclusions: Estradiol modulated the immune response of P. berghei ANKA-infected CBA mice, decreasing parasitemia and increasing NOS activity, and impacted negatively on survival and CM incidence, showing that neuroimmunoendocrine interactions are important in the physiopathogenesis of malaria infections.Universidade Federal do Pará. Núcleo de Medicina Tropical. Belém, PA, Brasil.Universidade Federal do Pará. Departamento de Patologia. Belém, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.Universidade Federal do Pará. Departamento de Fisiologia. Belém, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Pará. Departamento de Fisiologia. 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dc.title.pt_BR.fl_str_mv Estradiol, but not dehydroepiandrosterone, decreases parasitemia and increases the incidence of cerebral malaria and the mortality in Plasmodium berghei ANKA-infected CBA mice
title Estradiol, but not dehydroepiandrosterone, decreases parasitemia and increases the incidence of cerebral malaria and the mortality in Plasmodium berghei ANKA-infected CBA mice
spellingShingle Estradiol, but not dehydroepiandrosterone, decreases parasitemia and increases the incidence of cerebral malaria and the mortality in Plasmodium berghei ANKA-infected CBA mice
Libonati, Rosana Maria Feio
Estradiol / administração & dosagem
Estradiol / uso diagnóstico
Parasitemia / parasitologia
Plasmodium berghei / parasitologia
Malária Cerebral / mortalidade
Malária Cerebral / parasitologia
Camundongos Endogâmicos CBA / parasitologia
Castração
title_short Estradiol, but not dehydroepiandrosterone, decreases parasitemia and increases the incidence of cerebral malaria and the mortality in Plasmodium berghei ANKA-infected CBA mice
title_full Estradiol, but not dehydroepiandrosterone, decreases parasitemia and increases the incidence of cerebral malaria and the mortality in Plasmodium berghei ANKA-infected CBA mice
title_fullStr Estradiol, but not dehydroepiandrosterone, decreases parasitemia and increases the incidence of cerebral malaria and the mortality in Plasmodium berghei ANKA-infected CBA mice
title_full_unstemmed Estradiol, but not dehydroepiandrosterone, decreases parasitemia and increases the incidence of cerebral malaria and the mortality in Plasmodium berghei ANKA-infected CBA mice
title_sort Estradiol, but not dehydroepiandrosterone, decreases parasitemia and increases the incidence of cerebral malaria and the mortality in Plasmodium berghei ANKA-infected CBA mice
author Libonati, Rosana Maria Feio
author_facet Libonati, Rosana Maria Feio
Cunha, Maristela G
Souza, José Maria de
Santos, Marcos V. N
Oliveira, Salma Gomes de
Daniel-Ribeiro, Cláudio Tadeu
Carvalho, Leonardo José de Moura
Nascimento, José L. M. do
author_role author
author2 Cunha, Maristela G
Souza, José Maria de
Santos, Marcos V. N
Oliveira, Salma Gomes de
Daniel-Ribeiro, Cláudio Tadeu
Carvalho, Leonardo José de Moura
Nascimento, José L. M. do
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Libonati, Rosana Maria Feio
Cunha, Maristela G
Souza, José Maria de
Santos, Marcos V. N
Oliveira, Salma Gomes de
Daniel-Ribeiro, Cláudio Tadeu
Carvalho, Leonardo José de Moura
Nascimento, José L. M. do
dc.subject.decsPrimary.pt_BR.fl_str_mv Estradiol / administração & dosagem
Estradiol / uso diagnóstico
Parasitemia / parasitologia
Plasmodium berghei / parasitologia
Malária Cerebral / mortalidade
Malária Cerebral / parasitologia
Camundongos Endogâmicos CBA / parasitologia
Castração
topic Estradiol / administração & dosagem
Estradiol / uso diagnóstico
Parasitemia / parasitologia
Plasmodium berghei / parasitologia
Malária Cerebral / mortalidade
Malária Cerebral / parasitologia
Camundongos Endogâmicos CBA / parasitologia
Castração
description Objective: The effect of castration and subsequent replacement of dehydroepiandrosterone (DHEA) or estradiol on parasitemia, mortality and incidence of cerebral malaria (CM) was evaluated in CBA mice infected with Plasmodium berghei ANKA. Methods: Female mice were castrated, and groups of 12–15 animals received daily injections of DHEA, estradiol or saline. Four days after the start of treatment, mice were inoculated with 1 ! 106 P. berghei ANKA-parasitized erythrocytes. DHEA treatment was continued during the 5 days after infection, and estradiol was administered during the follow-up. Parasitemia was evaluated daily in Giemsastained blood smears. Signs of CM were determined by the manifestation of coma, limb paralysis and/or convulsions. Plasma TNF- levels were evaluated by sandwich ELISA. Nitric oxide synthase (NOS) activity in the brain of moribund mice was measured by the method of Bredt and Snyder. Results: In non-castrated infected mice, the incidence of CM was 50%, and plasma TNF- increased and brain NOS activity decreased compared to non-infected controls. Castration had no major effect on the parameters analyzed (parasitemia, mortality, CM incidence, TNF- levels or NOS activity). Estradiol replacement caused a decrease in parasitemia but resulted in higher CM incidence and faster mortality, with an increase in NOS activity. Conclusions: Estradiol modulated the immune response of P. berghei ANKA-infected CBA mice, decreasing parasitemia and increasing NOS activity, and impacted negatively on survival and CM incidence, showing that neuroimmunoendocrine interactions are important in the physiopathogenesis of malaria infections.
publishDate 2006
dc.date.issued.fl_str_mv 2006
dc.date.accessioned.fl_str_mv 2016-01-26T11:43:20Z
dc.date.available.fl_str_mv 2016-01-26T11:43:20Z
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dc.identifier.citation.fl_str_mv LIBONATI, Rosana Maria Feio et al. Estradiol, but not dehydroepiandrosterone, decreases parasitemia and increases the incidence of cerebral malaria and the mortality in Plasmodium berghei ANKA-infected CBA mice. NeuroImmunoModulation, v. 13, n. 1, p. 28-35, 2006
dc.identifier.uri.fl_str_mv https://patua.iec.gov.br/handle/iec/1053
dc.identifier.issn.-.fl_str_mv 1021-7401
dc.identifier.doi.-.fl_str_mv 10.1159/000093271
identifier_str_mv LIBONATI, Rosana Maria Feio et al. Estradiol, but not dehydroepiandrosterone, decreases parasitemia and increases the incidence of cerebral malaria and the mortality in Plasmodium berghei ANKA-infected CBA mice. NeuroImmunoModulation, v. 13, n. 1, p. 28-35, 2006
1021-7401
10.1159/000093271
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