Clonal dissemination of highly virulent Serratia marcescens strains producing KPC-2 in food-producing animals

Detalhes bibliográficos
Autor(a) principal: Valiatti, Tiago Barcelos
Data de Publicação: 2023
Outros Autores: Bessa Neto, Francisco Ozório, Santos, Fernanda Fernandes, Silva, Ramon Giovanni Brandão, Veiga, Ruanita, Corsi, Dandara Cassu, Moura, Tuane Carolina Ferreira, Lobato, Amalia Raiana Fonseca, Pignatari, Antonio Carlos Campos, Souza, Cintya Oliveira, Brasiliense, Danielle Muruci, Cayô, Rodrigo, Gales, Ana Cristina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
Texto Completo: https://patua.iec.gov.br/handle/iec/6845
Resumo: Serratia marcescens is a Gram-negative bacterium presenting intrinsic resistance to polymyxins that has emerged as an important human pathogen. Although previous studies reported the occurrence of multidrug-resistance (MDR) S. marcescens isolates in the nosocomial settings, herein, we described isolates of this extensively drug-resistant (XDR) species recovered from stool samples of food-producing animals in the Brazilian Amazon region. Three carbapenem-resistant S. marcescens strains were recovered from stool samples of poultry and cattle. Genetic similarity analysis showed that these strains belonged to the same clone. Whole-genome sequencing of a representative strain (SMA412) revealed a resistome composed of genes encoding resistance to β-lactams [blaKPC-2, blaSRT-2], aminoglycosides [aac(6′)-Ib3, aac(6′)-Ic, aph(3′)-VIa], quinolones [aac(6′)-Ib-cr], sulfonamides [sul2], and tetracyclines [tet(41)]. In addition, the analysis of the virulome demonstrated the presence of important genes involved in the pathogenicity of this species (lipBCD, pigP, flhC, flhD, phlA, shlA, and shlB). Our data demonstrate that food-animal production can act as reservoirs for MDR and virulent strains of S. marcescens.
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spelling Valiatti, Tiago BarcelosBessa Neto, Francisco OzórioSantos, Fernanda FernandesSilva, Ramon Giovanni BrandãoVeiga, RuanitaCorsi, Dandara CassuMoura, Tuane Carolina FerreiraLobato, Amalia Raiana FonsecaPignatari, Antonio Carlos CamposSouza, Cintya OliveiraBrasiliense, Danielle MuruciCayô, RodrigoGales, Ana Cristina2023-07-17T13:12:03Z2023-07-17T13:12:03Z2023VALIATTI, Tiago Barcelos et al. Clonal dissemination of highly virulent Serratia marcescens strains producing KPC-2 in food-producing animals. One Health, v. 17, n. 100591, Dec. 2023. DOI: https://doi.org/10.1016/j.onehlt.2023.100591. Disponível em: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10302155/pdf/main.pdf.2352-7714https://patua.iec.gov.br/handle/iec/684510.1016/j.onehlt.2023.100591Serratia marcescens is a Gram-negative bacterium presenting intrinsic resistance to polymyxins that has emerged as an important human pathogen. Although previous studies reported the occurrence of multidrug-resistance (MDR) S. marcescens isolates in the nosocomial settings, herein, we described isolates of this extensively drug-resistant (XDR) species recovered from stool samples of food-producing animals in the Brazilian Amazon region. Three carbapenem-resistant S. marcescens strains were recovered from stool samples of poultry and cattle. Genetic similarity analysis showed that these strains belonged to the same clone. Whole-genome sequencing of a representative strain (SMA412) revealed a resistome composed of genes encoding resistance to β-lactams [blaKPC-2, blaSRT-2], aminoglycosides [aac(6′)-Ib3, aac(6′)-Ic, aph(3′)-VIa], quinolones [aac(6′)-Ib-cr], sulfonamides [sul2], and tetracyclines [tet(41)]. In addition, the analysis of the virulome demonstrated the presence of important genes involved in the pathogenicity of this species (lipBCD, pigP, flhC, flhD, phlA, shlA, and shlB). Our data demonstrate that food-animal production can act as reservoirs for MDR and virulent strains of S. marcescens.National Council for Science and Technological Development (CNPq) and the Bill & Melinda Gates Foundation (process numbers 402659/2018-0, 443805/2018-0, and OPP1193112). Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) (Process number: 312066/2019-8).Universidade Federal de São Paulo. Escola Paulista de Medicina. Department of Internal Medicine. Division of Infectious Diseases Laboratório Alerta. São Paulo, SP, Brazil.Universidade Federal de São Paulo. Escola Paulista de Medicina. Department of Internal Medicine. Division of Infectious Diseases Laboratório Alerta. São Paulo, SP, Brazil / Universidade Federal de São Paulo (UNIFESP), Instituto de Ciências Ambientais, Químicas e Farmacêuticas. Departamento de Ciências Biológicas. Setor de Biologia Molecular, Microbiologia e Imunologia. Laboratório de Imunologia e Bacteriologia. Diadema, SP, Brazil.Universidade Federal de São Paulo. Escola Paulista de Medicina. Department of Internal Medicine. Division of Infectious Diseases Laboratório Alerta. São Paulo, SP, Brazil.Universidade Federal de São Paulo. Escola Paulista de Medicina. Department of Internal Medicine. Division of Infectious Diseases Laboratório Alerta. São Paulo, SP, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Universidade Federal de São Paulo. Escola Paulista de Medicina. Department of Internal Medicine. Division of Infectious Diseases Laboratório Alerta. São Paulo, SP, Brazil / Universidade Federal de São Paulo. Escola Paulista de Medicina. Department of Internal Medicine. Division of Infectious Diseases. Laboratório Especial de Microbiologia Clínica. São Paulo, SP, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Universidade Federal de São Paulo. Escola Paulista de Medicina. Department of Internal Medicine. Division of Infectious Diseases Laboratório Alerta. São Paulo, SP, Brazil / Universidade Federal de São Paulo (UNIFESP), Instituto de Ciências Ambientais, Químicas e Farmacêuticas. Departamento de Ciências Biológicas. Setor de Biologia Molecular, Microbiologia e Imunologia. Laboratório de Imunologia e Bacteriologia. Diadema, SP, Brazil.Universidade Federal de São Paulo. Escola Paulista de Medicina. Department of Internal Medicine. Division of Infectious Diseases Laboratório Alerta. São Paulo, SP, Brazil / Universidade Federal de São Paulo. Escola Paulista de Medicina. Department of Internal Medicine. Division of Infectious Diseases. Laboratório Especial de Microbiologia Clínica. São Paulo, SP, Brazil.engElsevierClonal dissemination of highly virulent Serratia marcescens strains producing KPC-2 in food-producing animalsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleSerratia marcescens / isolamento & purificaçãoSerratia marcescens / patogenicidadeGenes MDRResistência a Múltiplos MedicamentosGenômicaDoenças Transmitidas por AlimentosVigilância em Saúde Públicainfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALClonal dissemination of highly virulent Serratia marcescens strains producing KPC-2 in food-producing animals.pdfClonal dissemination of highly virulent Serratia marcescens strains producing KPC-2 in food-producing animals.pdfapplication/pdf1554975https://patua.iec.gov.br/bitstreams/66185cc0-2aa0-47fd-a64b-5e020523112f/download1d418fd14a420894b46e8129f63b68beMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82182https://patua.iec.gov.br/bitstreams/b543e02a-2939-4206-b922-7573184e0e6d/download11832eea31b16df8613079d742d61793MD52TEXTClonal dissemination of highly virulent Serratia marcescens strains producing KPC-2 in food-producing animals.pdf.txtClonal dissemination of highly virulent Serratia marcescens strains producing KPC-2 in food-producing animals.pdf.txtExtracted texttext/plain46830https://patua.iec.gov.br/bitstreams/7b34c828-5d3f-418b-a834-5e539a7206ae/downloadff0dcbac769484fe901a685d19ef09b1MD53THUMBNAILClonal dissemination of highly virulent Serratia marcescens strains producing KPC-2 in food-producing animals.pdf.jpgClonal dissemination of highly virulent Serratia marcescens strains producing KPC-2 in food-producing animals.pdf.jpgGenerated Thumbnailimage/jpeg5760https://patua.iec.gov.br/bitstreams/be8f2f48-120d-4f51-9c36-55ab1522e45a/downloadc1adf0b7d30ef0f3ab1c0ecff0f6a663MD54iec/68452023-07-17 14:00:11.014oai:patua.iec.gov.br:iec/6845https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2023-07-17T14:00:11Repositório Digital do Instituto Evandro Chagas (Patuá) - 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dc.title.pt_BR.fl_str_mv Clonal dissemination of highly virulent Serratia marcescens strains producing KPC-2 in food-producing animals
title Clonal dissemination of highly virulent Serratia marcescens strains producing KPC-2 in food-producing animals
spellingShingle Clonal dissemination of highly virulent Serratia marcescens strains producing KPC-2 in food-producing animals
Valiatti, Tiago Barcelos
Serratia marcescens / isolamento & purificação
Serratia marcescens / patogenicidade
Genes MDR
Resistência a Múltiplos Medicamentos
Genômica
Doenças Transmitidas por Alimentos
Vigilância em Saúde Pública
title_short Clonal dissemination of highly virulent Serratia marcescens strains producing KPC-2 in food-producing animals
title_full Clonal dissemination of highly virulent Serratia marcescens strains producing KPC-2 in food-producing animals
title_fullStr Clonal dissemination of highly virulent Serratia marcescens strains producing KPC-2 in food-producing animals
title_full_unstemmed Clonal dissemination of highly virulent Serratia marcescens strains producing KPC-2 in food-producing animals
title_sort Clonal dissemination of highly virulent Serratia marcescens strains producing KPC-2 in food-producing animals
author Valiatti, Tiago Barcelos
author_facet Valiatti, Tiago Barcelos
Bessa Neto, Francisco Ozório
Santos, Fernanda Fernandes
Silva, Ramon Giovanni Brandão
Veiga, Ruanita
Corsi, Dandara Cassu
Moura, Tuane Carolina Ferreira
Lobato, Amalia Raiana Fonseca
Pignatari, Antonio Carlos Campos
Souza, Cintya Oliveira
Brasiliense, Danielle Muruci
Cayô, Rodrigo
Gales, Ana Cristina
author_role author
author2 Bessa Neto, Francisco Ozório
Santos, Fernanda Fernandes
Silva, Ramon Giovanni Brandão
Veiga, Ruanita
Corsi, Dandara Cassu
Moura, Tuane Carolina Ferreira
Lobato, Amalia Raiana Fonseca
Pignatari, Antonio Carlos Campos
Souza, Cintya Oliveira
Brasiliense, Danielle Muruci
Cayô, Rodrigo
Gales, Ana Cristina
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Valiatti, Tiago Barcelos
Bessa Neto, Francisco Ozório
Santos, Fernanda Fernandes
Silva, Ramon Giovanni Brandão
Veiga, Ruanita
Corsi, Dandara Cassu
Moura, Tuane Carolina Ferreira
Lobato, Amalia Raiana Fonseca
Pignatari, Antonio Carlos Campos
Souza, Cintya Oliveira
Brasiliense, Danielle Muruci
Cayô, Rodrigo
Gales, Ana Cristina
dc.subject.decsPrimary.pt_BR.fl_str_mv Serratia marcescens / isolamento & purificação
Serratia marcescens / patogenicidade
Genes MDR
Resistência a Múltiplos Medicamentos
Genômica
Doenças Transmitidas por Alimentos
Vigilância em Saúde Pública
topic Serratia marcescens / isolamento & purificação
Serratia marcescens / patogenicidade
Genes MDR
Resistência a Múltiplos Medicamentos
Genômica
Doenças Transmitidas por Alimentos
Vigilância em Saúde Pública
description Serratia marcescens is a Gram-negative bacterium presenting intrinsic resistance to polymyxins that has emerged as an important human pathogen. Although previous studies reported the occurrence of multidrug-resistance (MDR) S. marcescens isolates in the nosocomial settings, herein, we described isolates of this extensively drug-resistant (XDR) species recovered from stool samples of food-producing animals in the Brazilian Amazon region. Three carbapenem-resistant S. marcescens strains were recovered from stool samples of poultry and cattle. Genetic similarity analysis showed that these strains belonged to the same clone. Whole-genome sequencing of a representative strain (SMA412) revealed a resistome composed of genes encoding resistance to β-lactams [blaKPC-2, blaSRT-2], aminoglycosides [aac(6′)-Ib3, aac(6′)-Ic, aph(3′)-VIa], quinolones [aac(6′)-Ib-cr], sulfonamides [sul2], and tetracyclines [tet(41)]. In addition, the analysis of the virulome demonstrated the presence of important genes involved in the pathogenicity of this species (lipBCD, pigP, flhC, flhD, phlA, shlA, and shlB). Our data demonstrate that food-animal production can act as reservoirs for MDR and virulent strains of S. marcescens.
publishDate 2023
dc.date.accessioned.fl_str_mv 2023-07-17T13:12:03Z
dc.date.available.fl_str_mv 2023-07-17T13:12:03Z
dc.date.issued.fl_str_mv 2023
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dc.identifier.citation.fl_str_mv VALIATTI, Tiago Barcelos et al. Clonal dissemination of highly virulent Serratia marcescens strains producing KPC-2 in food-producing animals. One Health, v. 17, n. 100591, Dec. 2023. DOI: https://doi.org/10.1016/j.onehlt.2023.100591. Disponível em: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10302155/pdf/main.pdf.
dc.identifier.uri.fl_str_mv https://patua.iec.gov.br/handle/iec/6845
dc.identifier.issn.-.fl_str_mv 2352-7714
dc.identifier.doi.pt_BR.fl_str_mv 10.1016/j.onehlt.2023.100591
identifier_str_mv VALIATTI, Tiago Barcelos et al. Clonal dissemination of highly virulent Serratia marcescens strains producing KPC-2 in food-producing animals. One Health, v. 17, n. 100591, Dec. 2023. DOI: https://doi.org/10.1016/j.onehlt.2023.100591. Disponível em: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10302155/pdf/main.pdf.
2352-7714
10.1016/j.onehlt.2023.100591
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